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AKTIVITAS ANTIBAKTERI DAN KLT BIOAUTOGRAFI EKSTRAK ETANOL DAUN SISIK NAGA (Drymoglossum piloselloides) TERHADAP Streptococcus mutans Ulfa, Evi Umayah; Sari, Desi Sandra; Wijaya, Dhani
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 10, No 1 (2013)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Sisik naga (Drymoglossum piloselloides) has been used empirically for dysentry and candidiasis. This diseases were caused by bacteria and fungi. The objective of this research was to evaluate the antibacterial effect and to find out the antimicrobial compound of sisik naga. Leaf of sisik naga was extracted with ethanol 90% by using maseration. This extract were tested for antibacterial activity against Streptococcus mutans using agar difussion. TLC Bioautography methods was used to identify the active compound. Result of antibacterial test showedf ethanolic extract of sisik naga had antibacterial effect against S. mutans. Bioautography showed one spot on Rf =0 give clear zone.

PENGEMBANGAN FORMULA KRIM MINYAK SEREH (Cymbopogon citratus) SEBAGAI ANTI NYAMUK TOPIKAL Ameliana, Lidya; Ulfa, Evi Umayah
STOMATOGNATIC- Jurnal Kedokteran Gigi Vol 9, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Volatile oils extracted by steam distillation from Cymbopogon citratus were formulated in creams. Theessential oils were then employed as active ingredients in the preparation of insect repellents used in our field trials.These creams containing 0%; 0,5%; 1%; 5%; 10% and 15% (w/w) of volatile oil from Cymbopogon citratus. Thesecreams were evaluated in physical qualities like appearance, pH, spreadibility, and viscosity. And also wereevaluated for their repellency effect against adult female mosquitoes of Aedes aegypti. Results from the field trialsindicate that cream containing 10% of the essential oil from Cymbopogon citratus provided 1071 Â± 26.514 seconds ofprotection time against mosquito bites. And the cream containing 15% of the essential oil from Cymbopogoncitratus provided 1097.333 Â± 19.139 seconds. It means cream containing 15% of the essential oil is the best formula ofrepellent cream, but does not significantly with cream containing 10 % of the essential oil

IDENTIFIKASI 16S RRNA DAN UJI ZIMOGRAFI BAKTERI ASAL PANTAI PAPUMA PENGHASIL ENZIM FIBRINOLITIK SEBAGAI ANTI ATHEROTHROMBOSIS A.S, Pananjung; I, Novanda asri; Nuryady, M Mirza; Ulfa, Evi Umayah
Program Kreativitas Mahasiswa - Penelitian PKM-P 2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Atherotrombosis is a disease caused by a blood clot (thrombus) in a blood vessel, and it can be the leading cause of death in the world. Atherotrombosis disease can be overcome with thrombolytic agents, one of which is a fibrinolytic enzyme but it has a expensive price, so we needto looking for a new fibrinolytic agent in this case the bacteria, from Papuma coastal. This research is a follow-up from past research, to identify the bacteria from papuma by using 16S rRNA, and to know the Enzyme activated by using Zymografi.The result of this research is, the bacteria identification by 16S rRNA is similar with Microbacterium testaceum with similarity percentage is about 97%. This bacteria is positive fibrinolityc bacteria it can showed by the result of Zymografi test, there is a one white band on zymografi gel, and because the protein marker on zymografi gel is unseen, so we just used theÂ qualitative observation. Keywords:Atherotrombosis, fibrinolitic, 16s rRNA, BacteriaÂ Identification, Zimografi.Â

KARAKTERISASI ISOLAT BAKTERI FIBRINOLITIK WU 021055* ASAL PERAIRAN PANTAI PAPUMA, JEMBER Sri Pananjung, Ajeng Maharani; Ulfa, Evi Umayah; Senjarini, Kartika; Arimurti, Sattya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 1 (2015): June 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

A blood clot (thrombus) in a blood stream is formed due to a circulatory system imbalance in the hemostasis which results in plug of blood vessels. The suppliy of nutrients and oxygen to the tissues is inhibited (ischemia) by the accumulation of thrombus and embolus in the blood vessel. This prosses is the main cause for further atherotrombotic diseases such as myocardial infraction and cerebral infraction. This disease could be overcome by thrombolytic therapy by using fibrinolyticÂ protease enzyme. Fibrinolytic activity of protease enzymes have been studied from various species of bacteria. Bacterial isolate of WU 021055* obtained from Papuma coastal waters has demonstrated fibrinolytic activity. This research was aimed to identify the bacterial isolate through morphological characterizationÂ (colony and cell morphology), physiological characterization (indole test, carbohydrates fermentation test (glucose, lactose, sucrose and fructose), catalase test, starch hydrolysis test, and the pH effect test), and molecular identificationÂ using 16S rRNA. Based on those characterizations, the bacterial isolate of WU 021055* shows a high similarity to Bacillus aerius.Keywords: Atherotrombosis, fibrinolytic, identification, characterization, bacteriaÂ ABSTRAKBekuan darah (trombus) dalam peredaran darah terbentuk akibat ketidakseimbangan sistem sirkulasi dalam hemostasis yang menyebabkan penyumbatan pembuluh darah. Akumulasi trombus dan embolus pada pembuluh darah mengakibatkan suplai nutrisi dan oksigen ke jaringan terhambat (iskemia) dan bahkan kematian jaringan (infark). Pembentukan ini merupakan etiologi dari penyakit aterotrombosis seperti infark miokard dan infark serebral. Penyakit akibat trombosis ini dapat diatasi dengan terapi trombolitik dengan enzim protease fibrinolitik. Aktivitas enzim protease fibrinolitik telah diteliti dari berbagai spesies bakteri. Isolat bakteri WU 021055* asal perairan pantai papuma tampak memiliki aktivitas fibrinolitik. Pada penelitian ini dilakukan identifikasiÂ isolat bakteri melalui karakterisasiÂ morfologi (morfologi koloni dan sel), karakterisasi fisiologis (uji indol, uji fermentasi karbohidrat (glukosa, laktosa, sukrosa dan fruktosa), uji katalase, uji hidrolisis pati, dan uji pengaruh pH), dan identifikasi secara molekuler menggunakan 16S rRNA. Berdasarkan karakterisasi morfologi, fisiologi, dan marker 16S rRNA, isolat bakteri WU 021055* menunjukkan kemiripan yang tinggi dengan Bacillus aerius.Kata kunci: Aterotrombosis, fibrinolitik, identifikasi, karakterisasi, bakteri

Aktivitas Sitotoksik Ekstrak n-Heksana, Diklorometana, dan Metanol Daun Beluntas (Pluchea indica Less.) terhadap Sel Kanker Leher Rahim (HeLa) Puspitasari, Endah; Agustina, Bayu; ., Nuri; Ulfa, Evi Umayah
Jurnal Farmasi Sains dan Terapan Vol 2, No 1 (2015)
Publisher : Jurnal Farmasi Sains dan Terapan

Eksplorasi agen kemopreventif kanker masih terus dikembangkan, terutama yang berasal dari bahan alam. Hal ini didasari oleh keinginan untuk menekan angka kematian akibat kanker dan mengurangi efek samping yang ditimbulkan oleh terapi kanker yang saat ini digunakan. Penelitian ini ditujukan untuk mengetahui aktivitas sitotoksik daun beluntas (Pluchea indica Less.) terhadap sel kanker leher rahim yang merupakan kanker ginekologi peringkat pertama yang menyerang wanita. Aktivitas sitotoksik dilakukan terhadap ekstrak n-heksana, diklorometana, dan metanol daun beluntas terhadap sel HeLa dengan metode perhitungan langsung menggunakan bantuan perwarna trypan blue. Nilai IC50 dari ketiga ekstrak daun beluntas berturut turut adalah 18,06 Î¼g/ml, 74,56 Î¼g/ml, dan 31,21 Î¼g/ml. Ketiganya memberikan perbedaan yang signifikan. Ketiga ekstrak yang diuji memiliki aktivitas sitotoksik dan dapat dikembangkan sebagai kandidat agen kemopreventif kanker. Namun masih perlu diteliti lebih lanjut bagaimana mekanisme aksi yang menjadi perantara aktivitas sitotoksik tersebut.

KARAKTERISASI ISOLAT BAKTERI FIBRINOLITIK WU 021055* ASAL PERAIRAN PANTAI PAPUMA, JEMBER Sri Pananjung, Ajeng Maharani; Ulfa, Evi Umayah; Senjarini, Kartika; Arimurti, Sattya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 1 (2015): June 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

A blood clot (thrombus) in a blood stream is formed due to a circulatory system imbalance in the hemostasis which results in plug of blood vessels. The suppliy of nutrients and oxygen to the tissues is inhibited (ischemia) by the accumulation of thrombus and embolus in the blood vessel. This prosses is the main cause for further atherotrombotic diseases such as myocardial infraction and cerebral infraction. This disease could be overcome by thrombolytic therapy by using fibrinolyticÃ‚Â protease enzyme. Fibrinolytic activity of protease enzymes have been studied from various species of bacteria. Bacterial isolate of WU 021055* obtained from Papuma coastal waters has demonstrated fibrinolytic activity. This research was aimed to identify the bacterial isolate through morphological characterizationÃ‚Â (colony and cell morphology), physiological characterization (indole test, carbohydrates fermentation test (glucose, lactose, sucrose and fructose), catalase test, starch hydrolysis test, and the pH effect test), and molecular identificationÃ‚Â using 16S rRNA. Based on those characterizations, the bacterial isolate of WU 021055* shows a high similarity to Bacillus aerius.Keywords: Atherotrombosis, fibrinolytic, identification, characterization, bacteriaÃ‚Â ABSTRAKBekuan darah (trombus) dalam peredaran darah terbentuk akibat ketidakseimbangan sistem sirkulasi dalam hemostasis yang menyebabkan penyumbatan pembuluh darah. Akumulasi trombus dan embolus pada pembuluh darah mengakibatkan suplai nutrisi dan oksigen ke jaringan terhambat (iskemia) dan bahkan kematian jaringan (infark). Pembentukan ini merupakan etiologi dari penyakit aterotrombosis seperti infark miokard dan infark serebral. Penyakit akibat trombosis ini dapat diatasi dengan terapi trombolitik dengan enzim protease fibrinolitik. Aktivitas enzim protease fibrinolitik telah diteliti dari berbagai spesies bakteri. Isolat bakteri WU 021055* asal perairan pantai papuma tampak memiliki aktivitas fibrinolitik. Pada penelitian ini dilakukan identifikasiÃ‚Â isolat bakteri melalui karakterisasiÃ‚Â morfologi (morfologi koloni dan sel), karakterisasi fisiologis (uji indol, uji fermentasi karbohidrat (glukosa, laktosa, sukrosa dan fruktosa), uji katalase, uji hidrolisis pati, dan uji pengaruh pH), dan identifikasi secara molekuler menggunakan 16S rRNA. Berdasarkan karakterisasi morfologi, fisiologi, dan marker 16S rRNA, isolat bakteri WU 021055* menunjukkan kemiripan yang tinggi dengan Bacillus aerius.Kata kunci: Aterotrombosis, fibrinolitik, identifikasi, karakterisasi, bakteri

KARAKTERISASI ISOLAT BAKTERI FIBRINOLITIK WU 021055* ASAL PERAIRAN PANTAI PAPUMA, JEMBER Ajeng Maharani Sri Pananjung; Evi Umayah Ulfa; Kartika Senjarini; Sattya Arimurti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 1 (2015): June 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

A blood clot (thrombus) in a blood stream is formed due to a circulatory system imbalance in the hemostasis which results in plug of blood vessels. The suppliy of nutrients and oxygen to the tissues is inhibited (ischemia) by the accumulation of thrombus and embolus in the blood vessel. This prosses is the main cause for further atherotrombotic diseases such as myocardial infraction and cerebral infraction. This disease could be overcome by thrombolytic therapy by using fibrinolyticÂ protease enzyme. Fibrinolytic activity of protease enzymes have been studied from various species of bacteria. Bacterial isolate of WU 021055* obtained from Papuma coastal waters has demonstrated fibrinolytic activity. This research was aimed to identify the bacterial isolate through morphological characterizationÂ (colony and cell morphology), physiological characterization (indole test, carbohydrates fermentation test (glucose, lactose, sucrose and fructose), catalase test, starch hydrolysis test, and the pH effect test), and molecular identificationÂ using 16S rRNA. Based on those characterizations, the bacterial isolate of WU 021055* shows a high similarity to Bacillus aerius.Keywords: Atherotrombosis, fibrinolytic, identification, characterization, bacteriaÂ ABSTRAKBekuan darah (trombus) dalam peredaran darah terbentuk akibat ketidakseimbangan sistem sirkulasi dalam hemostasis yang menyebabkan penyumbatan pembuluh darah. Akumulasi trombus dan embolus pada pembuluh darah mengakibatkan suplai nutrisi dan oksigen ke jaringan terhambat (iskemia) dan bahkan kematian jaringan (infark). Pembentukan ini merupakan etiologi dari penyakit aterotrombosis seperti infark miokard dan infark serebral. Penyakit akibat trombosis ini dapat diatasi dengan terapi trombolitik dengan enzim protease fibrinolitik. Aktivitas enzim protease fibrinolitik telah diteliti dari berbagai spesies bakteri. Isolat bakteri WU 021055* asal perairan pantai papuma tampak memiliki aktivitas fibrinolitik. Pada penelitian ini dilakukan identifikasiÂ isolat bakteri melalui karakterisasiÂ morfologi (morfologi koloni dan sel), karakterisasi fisiologis (uji indol, uji fermentasi karbohidrat (glukosa, laktosa, sukrosa dan fruktosa), uji katalase, uji hidrolisis pati, dan uji pengaruh pH), dan identifikasi secara molekuler menggunakan 16S rRNA. Berdasarkan karakterisasi morfologi, fisiologi, dan marker 16S rRNA, isolat bakteri WU 021055* menunjukkan kemiripan yang tinggi dengan Bacillus aerius.Kata kunci: Aterotrombosis, fibrinolitik, identifikasi, karakterisasi, bakteri

Uji Aktivitas Antibakteri dan Antibiofilm Minyak Atsiri Rimpang Bengle (Zingiber purpureum Roscoe) terhadap Bakteri Staphylococcus epidermidis (Antibacterial and Antibiofilm Activity Test of Bengle Rhizome Essential Oil (Zingiber purpureum Roscoe) agains Hidayatul Ulyah; Evi Umayah Ulfa; Endah Puspitasari
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Staphylococcus epidermidis has become one of the most important pathogenic bacteria that lead to nosocomial infection. This is caused by its ability to form a biofilm on the surface. Essential oils are one of the natural product that could be used as antiinfection agents. Bengle rhizome essential oil has been known to have activity against non-pathogenic bacteria S. epidermidis. This study was proposed to know the antibacterial and antibiofilm activity of bengle rhizome essential oil against clinical isolate of S. epidermidis at concentrations of 45, 60, 75, and 90%. Antibacterial activity was determined by the diffusion method with hole plate technique, while antibiofilm activity was determined by the microtiter assay method with crystal violet staining and quantified by measuring the optical density (OD) at λ 595 nm. This study showed that bengle rhizome essential oil has antibacterial and antibiofilm activity. The largest antibacterial and antibiofilm activity was shown from 90% concentration of bengle rhizome essential oil. Keywords: nosocomial infections, antibacterial, antibiofilm, bengle essential oil, Staphylococcus epidermidis.

Pengaruh Ekstrak Metanol Daun Kayu Kuning (Arcangelisia flava (L.) Merr.) terhadap Kadar Kolesterol Total dan Trigliserida Tikus Hiperlipidemia (The Influence of Methanol Extract of Yellow Root (Arcangelisia flava (L.) Merr.) Leaves on Total Cholesterol Putri Eka Maryani; Evi Umayah Ulfa; Ema Rachmawati
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Hyperlipidemia is heterogeneous disorder that characterized by elevated levels of total cholesterol, low density lipoprotein (LDL), very low density lipoprotein (VLDL), triglyceride, and decreased levels of high density lipoprotein (HDL). The aim of this study was to determine the ability of methanol extract of leaves of Arcangelisia flava to decrease total cholesterol and triglyceride in some variations of dosage. Five groups experimental animal (each consisting of 4 rats) which have been induced by high fat and fructose diet for 45 days, followed by oral treatment of extract at a dose of 250 mg/kg BW, 500 mg/kg BW, 750 mg/kg BW, simvastatin 0.36 mg/200 g BW (control (+)), and CMC Na 1% (control (-)) for 7 days. The blood was taken on day 8, then was measured the total cholesterol and triglyceride. The test results showed that extract could decrease total cholesterol and triglyceride in line with increasing doses. According to ANOVA, there are significant differences between treatment groups (p

Pengaruh Ekstrak Metanol Daun Kayu Kuning (Arcangelisia flava (L.) Merr) terhadap Histopatologi Aorta Tikus Wistar Hiperlipidemia (The Influence of Methanol Extract of Yellow Root (Arcangelisia flava (L.) Merr) Leaves on Aortic Histopathology in Hyperli Yuniar Wahyu Rahmawati; Evi Umayah Ulfa; Ema Rachmawati
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Atherosclerosis is the leading cause of heart attack and stroke. Hyperlipidemia is one of the triggers of atherosclerosis through oxidative stress and formation of foam cells. Atherogenic index (the ratio of LDL/HDL) is a predictive parameter of coronary heart disease. A.flava is potential as antiaterosklerosis due to the content of berberine and flavonoids. This study was undertaken to determine the ability of methanolic extract of A.flava leaves (EMDAf) in lowering atherogenic index and the number of foam cells. Five groups experimental animals (each consisting of 4 rats) was induced with high fat and fructose diet for 45 days, followed by administration of EMDAf dose of 250 mg/kg BW, 500 mg/kg BW, 750 mg/kg BW, simvastatin 1.8 mg/kg BW (control (+)), and CMC Na 1% (control (-)) for 7 days treatments. Then the rat arcus aorta was taken for histopathological examination with Masson Trichrome staining. ANOVA test results showed that EMDAf could decrease the atherogenic index, but not significantly (p> 0.05). While the Kruskall Wallis test results showed that the increase dose of EMDAf might improve histopathology of the aorta through a reduction in the number of foam cells significantly compared to the negative control (p <0.05). Berberine contents in EMDAf is 0.046% measured by TLC-densitometry. It could be concluded that methanolic extract of A.flava leaves had the ability to decrease in the atherogenic index value and the number of foam cells. Keywords: Arcangelisia flava leaves, foam cells, atherogenic index, aorta, histopathology

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