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Isolation and Identification of Biosurfactant Producing Bacteria From Workshop Wastewater Asri Ainul; M. Hasbi; Eko Purwanto
Ilmu Perairan (Aquatic Science) Vol 9, No 1 (2021)
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.9.1.p.31-37

Abstract

Automotive workshop activities produce oil ills that may pollute waters around the workshop area. The oil-polluted water may inhabit biosurfactant producing bacteria that are able to degrade the oil. A study aimed to isolate and identify the bacteria has been conducted from July to September 2020. The bacteria samples were sampled from workshop wastewater at Kubang Raya street KM 2,5 Pekanbaru, Riau Province and sampling were conducted three times. The bacteria were isolated using TSB (Tryptone Soy Broth) and TSA (Tryptone Soy Agar) media and were identified by using biochemical methods. Results showed that there were seven types of bacteria, namely Providencia, Proteus, Acinetobacter, Bacillus, Aeromonas, Proteus and Serratia. The Emulsification index of  Providencia was 38.8%, Proteus 50%, Acinetobacter 48.8%, Bacillus 52,1%, Aeromonas 47,6%, Proteus 54,7% and Serratia 48,8%. Data obtained from this study showed that all of the identified bacteria are able to produce biosurfactants.
Isolation and Identification of Biosurfactant Producing Bacteria from Slaughterhouse Wastewater Kristina Sinaga; M. Hasbi; Eko Purwanto
Ilmu Perairan (Aquatic Science) Vol 9, No 1 (2021)
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.9.1.p.49-55

Abstract

The slaughterhouse wastewater is rich of oil and fat and it may inhabit by biosurfactant producing bacteria. Isolation of the bacteria is needed in order to find out a biosurfactant producing bacteria that can be used to maintain the oil and fat polluted area through bioremediation process. To isolate and identify the biosurfactant-producing bacteria, a study has been conducted on July to September 2020. The samples were obtained from slaughterhouse wastewater at Cipta Karya street Pekanbaru, Riau Province and samplings were conducted three times. The isolation was conducted by TSB (Tryptic Soy Broth)  and incubated for 24 hours. Then the bacterial samples were planted by TSA (Tryptic Soy Agar)  and isolated for 24 hours. Then, it was identified using biochemical and morphological tests were carried out to find out the types of bacteria. The Emulsification Index was then calculated by using TSB (Tryptic Soy Broth) and kerosene. Results shown that the emulsification index were Flavobacterium 53%, Agrobacterium 53%, Serratia 60%, Salmonella 52. 5%, Salmonella 52.4%, Proteus 65.7%, Clostridium 62% and Aeromonas 59.5%. As Proteus is shown the highest Emulsification Index, it was check using a PCR (Polymerase Chain Reaction) and it is proved that the species is Proteus vulgaris
Identification of Microplastics in Common Carp (Cyprinus carpio) at Koto Panjang Reservoir Kampar Regency Riau Province Dwi Astria Ulfa; Eko Purwanto; Budijono Budijono
Jurnal Perikanan dan Kelautan Vol 27, No 2 (2022): June 2022
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.2.145-150

Abstract

In the Koto Panjang Reservoir the microplastic may originated from the degraded plastic waste as well as degraded plastic net from the fish culturing cages. The microplastic may be accidentally ingulfed as fish swallowing their food. To understand the present of microplastic present in the stomach of wild common carps living around the fish net cage, a study was conducted on in December 2020- February 2021. The fish were captured using fish net (3-5 inc mesh size), 3 times, once/ 2 weeks. The fish stomach was removed and the presence of the microplastic was identified. The stomach content of the fish was mixed with 10% KOH (minimum 3 x of stomach content volume) and was incubated for2 weeks to dilute the organic materials. Then the presence of microplastic was observed using a binocular microscope. Types and abundance of the microplastic was noted. Results shown that the types of microplastic present were   films, fibers and fragments. The highest type of microplastic found in the stomach of the fish was films  (2.3 particles/ fish), followed by fibers (0.6 particles/fish) and fragments  (1.1 particles/ fish).
Profil Kualitas Air Sumur Akibat Intrusi Air Laut di Kawasan Pantai Air Manis Kota Padang Nabila Qathrunnada; Dwi Puryanti; Eko Purwanto
Jurnal Fisika Unand Vol 12 No 1 (2023)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (722.412 KB) | DOI: 10.25077/jfu.12.1.144-150.2023

Abstract

Penelitian ini bertujuan untuk mengetahui kualitas dan tingkat pencemaran air sumur akibat intrusi air laut di kawasan Pantai Air Manis melalui pengujian beberapa parameter pencemaran. Pengujian nilai konduktivitas listrik dan Total Dissolved Solid (TDS) dilakukan untuk menentukan tingkat keasinan air sumur. Pengujian Total Suspend Solid (TSS), pH dan temperatur dilakukan untuk mengetahui kualitas air sumur. Nilai konduktivitas listrik yang didapatkan dari 15 sumur berkisar antara 47,33 µS/cm - 719,67 µS/cm dimana nilai ini termasuk kategori air tawar. Dari hasil pengujian TDS, terdapat 2 sampel yang melebihi standar air tawar menurut Panitia Ad Hoc Intrusi Air Asin (PAHIAA) yaitu sampel 1 (1265,5 mg/L) dan sampel 2 (1028 mg/L) dimana lokasi sampel ini sangat dekat dengan pantai di arah barat.  Kecilnya indikasi adanya intrusi air laut dikarenakan lokasi yang dekat dengan perbukitan yang dapat menjadi daerah resapan sehingga air tanah mengalir ke arah laut dengan tekanan tinggi. Hasil pengujian nilai TSS menunjukkan terdapat 1 sampel yang melebihi standar mutu air bersih. Sedangkan berdasarkan hasil pengujian pH dan temperatur, 15 sampel air sumur masih memenuhi standar mutu air bersih. Nilai Indeks Pencemaran (IP) rata-rata air sumur pada lokasi penelitian adalah 0,6050 sehingga dapat dikategorikan dalam kondisi tidak tercemar.
Identifikasi Pencemaran Air Laut di Pesisir Pantai Sungai Pisang Kota Padang Ditinjau dari Parameter Fisika dan Kimia Muhammad Rinaldi; Dwi Puryanti; Eko Purwanto
Jurnal Fisika Unand Vol 13 No 5 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jfu.13.5.690-696.2024

Abstract

This research aims to identify seawater pollution by determining the concentration of heavy metals (Pb, Cd, and Cu), electrical conductivity, total dissolved solids (TDS), total suspended solids (TSS), temperature, and pH in seawater samples from the coast of Sungai. Pisang, Padang City. Heavy metal concentration values were measured using an Atomic Absorption Spectroscopy (AAS), electrical conductivity was measured using a conductivity meter, TDS and TSS were determined using the gravimetric method, temperature was measured using a thermometer, and pH was measured using a pH meter. From the research results, the highest pH value was obtained at 7.8, the highest temperature value was 33 ℃, the highest electrical conductivity value was 163.6 µS/cm and the highest TSS value was 22.6 mg/L. The average values of pH, temperature, electrical conductivity and TSS obtained did not exceed the quality standard limits based on Minister of the Environment Regulation Number 51 of 2004. The highest TDS value obtained was 3821 mg/L and did not exceed the quality standard limits based on Republic of Indonesia Government Regulation Number 82 of 2001. The concentration values of the heavy metals Pb, Cd, and Cu on the coast of the Sungai Pisang did not exceed the quality standard limits based on Minister of Environment Regulation Number 51 of 2004. From the research results it can be concluded that the sea water on the coast of the Sungai Pisang not polluted.
Sosialisasi Serbuk Penjernih Air Gambut di Kelurahan Industri Tenayan Kecamatan Tenayan Raya Kota Pekanbaru Budijono Budijono; Isma Mulyani; Yuliati Yuliati; Eko Purwanto; Andri Hendrizal; Yudho Harjoyudanto; Rina D'rita Sibagariang
CANANG: Jurnal Pengabdian Masyarakat Vol 4, No 1 (2024)
Publisher : PELANTAR PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/canang.v4i1.45

Abstract

Riau is known for its peaty waters, which often pose a challenge in providing clean water to the community. This activity aims to introduce a simple yet effective technology in purifying peat water using specially formulated magic powder. The methods used in this activity include counseling, demonstration of the use of peat water purification magic powder, and interactive discussions with participants. The participants of this activity were the local community who were very enthusiastic about participating in the whole series of events. The results of this activity showed an increase in community knowledge and understanding of the importance of clean water quality and an easy way to purify peat water using magic powder. Evaluation of the participants' understanding was conducted through questionnaires before and after the activity. The evaluation results showed that most participants were able to understand and apply the peat water purification method correctly. This activity is expected to be sustainable and have a positive impact on the health and quality of life of the community in Tenayan Industrial Village.
Identification of Microplastics in Common Carp (Cyprinus carpio) at Koto Panjang Reservoir Kampar Regency Riau Province Dwi Astria Ulfa; Eko Purwanto; Budijono Budijono
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.2.145-150

Abstract

In the Koto Panjang Reservoir, the microplastic may originate from the degraded plastic waste as well as degraded plastic net from the fish culturing cages. The microplastic may be accidentally ingulfed as fish swallowing their food. To understand the presence of microplastic present in the stomach of wild common carps living around the fish net cage, a study was conducted in December 2020-February 2021. The fish were captured using fish net (3-5 inc mesh size), 3 times, once/ 2 weeks. The fish stomach was removed and the presence of the microplastic was identified. The stomach content of the fish was mixed with 10% KOH (minimum 3 x of stomach content volume) and was incubated for2 weeks to dilute the organic materials. Then the presence of microplastic was observed using a binocular microscope. Types and abundance of the microplastic was noted. Results shown that the types of microplastic present were films, fibers and fragments. The highest type of microplastic found in the stomach of the fish was films (2.3 particles/ fish), followed by fibers (0.6 particles/fish) and fragments (1.1 particles/ fish).
Kandungan Mikroplastik pada Air Berdasarkan Kedalaman di Waduk PLTA Koto Panjang, Kabupaten Kampar, Provinsi Riau Ade Friadi J; Eko Purwanto; Budijono Budijono
Jurnal Fisika Unand Vol 12 No 3 (2023)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jfu.12.3.437-443.2023

Abstract

Tujuan penelitian ini adalah untuk mengetahui tipe dan kelimpahan mikroplastik pada perairan waduk PLTA Koto Panjang. Penelitian ini dilaksanakan pada November 2021-Januari 2022 di perairan Waduk PLTA Koto Panjang. Pengambilan sampel dilakukan secara purposive sampling pada 5 stasiun penelitian. Analisis sampel dan identifikasi mikroplastik di Laboratorium Pengolahan Limbah Fakultas Perikanan dan Kelautan Universitas Riau. Hasil penelitian menunjukkan bahwa tipe mikroplastik yang teridentifikasi adalah fiber, fragmen dan film. Tipe yang paling dominan ditemukan adalah  tipe  film.  Kelimpahan  mikroplastik paling  banyak ditemukan  di stasiun  5 dengan  kelimpahan  tertinggi  pada  kedalaman  3 meter  dibawah  permukaan  air sebesar 1.267 partikel/ m3.
Hubungan Total Padatan Tersuspensi (TSS) dengan Klorofil-a di Perairan Pesisir Kecamatan Dumai Barat, Kota Dumai, Riau Nadia Putri Julita; Asmika Harnalin Simarmata; Eko Purwanto
Jurnal Ilmu Perairan (Aquatic Science) Vol. 11 No. 3 (2023): November
Publisher : LPPM Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Aktivitas DAS di Sungai Dumai dan alih fungsi lahan pada Muara Sungai Masjid akan mempengaruhi kualitas perairan pesisir Dumai salah satunya adalah peningkatan padatan tersuspensi. TSS adalah padatan dalam air, terdiri dari komponen biotik seperti fitoplankton, zooplankton, bakteri, fungi, ataupun komponen abiotik seperti detritus, pasir, lumpur, tanah liat dan partikel anorganik dengan ukuran berkisar 0,004-1,0 mm. Penelitian ini bertujuan untuk mengetahui hubungan TSS dengan klorofil-a di perairan Pesisir Dumai, yang dilaksanakan pada bulan Januari-Februari 2023 dengan metode survei. Sampling dilakukan sebanyak 3 kali dengan interval waktu 2 minggu. Metode analisis TSS dilakukan secara gravimetri dan klorofil-a secara ekstraksi aseton. Parameter kualitas perairan seperti suhu, kecepatan arus, kecerahan, pH, salinitas, DO, CO2, nitrat, fosfat. Hasil penelitian, rata-rata TSS berkisar 30,7-91 mg/L. Rata-rata klorofil-a berkisar 20,85-85,67 µg/L. Parameter kualitas air seperti suhu 28,2-28,5 oC, kecepatan arus 0,44-1,09 m/s, kecerahan 0,22-0,43 m, pH 5,7-7, salinitas 13-26 ppt, DO 5,17-7,78 mg/L, CO2: 5,28-12,54 mg/L, nitrat 0,05-0,09 mg/L, fosfat 0,13-0,28 mg/L. Hubungan TSS dengan klorofil-a, y=-0,6955x+93,048 dengan koefisien R2 yaitu 0,45 yang berarti pengaruh TSS terhadap klorofil-a sedang, dimana 45% dipengaruhi TSS sedangkan 55% dipengaruhi faktor lain.
Identifikasi Jenis dan Kelimpahan Mikroplastik pada Sedimen di Danau Buatan Bandar Kayangan Lembah Sari Kecamatan Rumbai Pesisir Kota Pekanbaru Regina Feby Cesilia; Eko Purwanto; Eni Sumiarsih
Jurnal Ilmu Perairan (Aquatic Science) Vol. 13 No. 1 (2025): Maret
Publisher : LPPM Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.13.1.p.142-149

Abstract

Mikroplastik adalah sampah plastik yang berukuran 0,3-5 mm dan dapat terakumulasi pada sedimen. Penelitian ini bertujuan untuk mengidentifikasi jenis mikroplastik dan kelimpahan mikroplastik yang terdapat pada sedimen di Danau Buatan Bandar Kayangan. Penelitian ini dilaksanakan pada bulan Oktober-November 2023. Penelitian ini menggunakan metode survei. Pengamatan sampel sedimen dilakukan di Laboratorium Kimia Laut, Fakultas Perikanan dan Kelautan Universitas Riau. Sampel sedimen diambil menggunakan pipa paralon 4 inchi dengan kedalaman 0-10 cm sebanyak 1 kg. Pengamatan mikroplastik dilakukan dengan beberapa tahap yaitu pengeringan, pemisahan densitas, penyaringan dan penghitungan dengan menggunakan mikroskop. Hasil penelitian menunjukkan bahwa jenis mikroplastik yang ditemukan ada tiga jenis yaitu fragmen (37%), fiber (33%) dan film (30%). Sedangkan kelimpahan mikroplastik tertinggi antar stasiun terdapat pada Stasiun 2 dengan kelimpahan 440,00 partikel/kg dan terendah pada Stasiun 1 dengan kelimpahan 357,78 partikel/kg. Perbedaan jumlah mikroplastik antar stasiun dilakukan analisis Uji T didapatkan menunjukkan bahwa setiap stasiun yang dibandingkan dengan Stasiun 3 tidak memiliki perbedaan, sedangkan antara Stasiun 1 dan Stasiun 2 memiliki perbedaan jumlah mikroplastik antar stasiun.