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All Journal HAYATI Journal of Biosciences Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Sumberdaya Hayati Quagga: Jurnal Pendidikan dan Biologi
Jepri Agung Priyanto
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology

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An Evaluasi Pelaksanaan Workshop Daring Penerapan Ilmu Biologi di SMA Plus Al Wahid Tasikmalaya Nisa Rachmania Mubarik; Mira Dewi; Jepri Agung Priyanto
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 8 No. 1 (2022): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.8.1.93-100

Abstract

The workshop on the application of Biology online (on a network) using the internet during the Covid-19 pandemic has been held at SMA Plus Al Wahid, Tasikmalaya Regency. This activity is in the context of a community service program entitled IPB lecturers to serve. The objectives of this activity are: 1) To describe the implementation of an online explanation workshop program for high school level Biology learning, especially those related to viruses and body immunity; 2) Explaining the level of understanding of the workshop participants before and after the presentation of the material; and 3) Explaining the results of the evaluation of participants' satisfaction with the delivery of workshop materials. The workshop was held on September 4, 2020 online using the Zoom application platform. The number of participants who took part in the workshop was 43 people, consisting of students in grades X, XI, and XII in the natural sciences, teachers, and the general public (non-teacher high school employees) from SMA Plus Al Wahid. Only 16 student participants who filled out the pre-test and post-test were used to evaluate this activity. Evaluation of the results of the pre-test and post-test using a one-group pre-test-post-test design using the N gain method or normalized gain analysis. The results of the workshop showed an increase in participants' understanding of the material on the application of Biology by 30.6% and participants showed a satisfaction level of 76.25%.
Paederia foetida Linn Leaves-Derived Extract Showed Antioxidant and Cytotoxic Properties Against Breast Carcinoma Cell Jepri Agung Priyanto; Muhammad Eka Prastya; Gian Primahana; Ahmad Randy; Diyah Tri Utami
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.271-280

Abstract

Paederia foetida Linn. is a rich source of active compounds, yet its antioxidant and cytotoxic properties remain poorly studied. This current research aimed to investigate the chemical profiles of a crude extract derived from P. foetida leaves, its fractionated substances, and their antioxidant and cytotoxic properties on MCF-7 cells, a breast carcinoma cell. Six fractionated substances have been separated in thin layer chromatography (TLC) plate. Five fractions showed antioxidant properties, as indicated by the formation of the yellowish band on the TLC plate after spraying with the DPPH reagent. Furthermore, crude extract of P. foetida leaves and its fractions are also characterized by antioxidant properties against 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. F1 fraction exhibited the most potent antioxidant activity with IC50 values of 10.94±2.67 and 50.04±0.48 µg/ml, respectively, toward DPPH and ABTS radicals. Crude extract and all six fractions also varied in their cytotoxic properties against MCF-7 cells, with IC50 values ranging from 410.24±0.30 to 831.57±6.91 µg/ml. F3 fractions showed the strongest cytotoxicity (IC50 value: 410.24±0.30 µg/ml). Methanolic extract and active plant leaf fractions contained flavonoid and phenolic compounds, as measured by aluminum chloride and Folin-Ciocalteu reagent, respectively. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis showed that phenol, flavonoid, alkaloid, limonoid, and steroid were the major compound found in the crude extract and fractions. In conclusion, the present study proved that crude extract of P. foetida leaves and its fractionated substances were potential as antioxidant and cytotoxic agents.
Seleksi dan Karakterisasi Bakteri Halotoleran Penghasil Enzim Amilase Dari Produk Fermentasi Ikan-Inasua: Selection and Characterization of Halotolerant Bacteria that produce Amylase Enzyme from Fish-Inasua Fermented Food Karina Eku Dwinanda Gunawan; Jepri Agung Priyanto; Nisa Rachmania Mubarik
Jurnal Sumberdaya Hayati Vol. 9 No. 3 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.3.96-103

Abstract

Fish-inasua fermented food is a potential source to obtain halotolerant bacteria-producing amylase. Previously, four bacterial isolates such as IG12, IG6, IG66, and IG31, isolated from this food, have been reported to be able to produce protease and lipase enzyme, but their potential as amylase enzyme producers have not been studied. This study aims to characterize and test the potential of halotolerant bacteria isolated from this food in producing amylase enzymes. Based on Gram staining, four bacterial isolates were Gram-positive. The qualitative test of amylase on NA medium with the addition of 1% starch showed four bacteria were able to produce amylase in NaCl (0 to 5%)-containing medium. IG66 isolate was the most potential isolate because it had the highest enzyme activity in a medium with 5% NaCl concentration, which was 0.095 U/ml. The amylase activity was produced maximum at the 8th hour, which is the early stationary phase. Its maximum activity in the 5% NaCl-containing medium was at the 10th hour. In addition, amylase produced by IG66 isolate reached optimum activity at a temperature of 50°C and pH 7. This study concluded that IG66 isolate was the most potential halotolerant isolate that could be developed as an amylase-producing agent.
Antibacterial Activity of Clove-derived Endophytic Bacteria of Niallia nealsonii DCL1 and Bacillus paranthracis DCL3 Nadya Nabila Qurani; Jepri Agung Priyanto; Rika Indri Astuti
Quagga: Jurnal Pendidikan dan Biologi Vol 18 No 1 (2026): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v18i1.470

Abstract

Pathogenic bacterial infection is still a major health problem that causes death. Endophytic bacteria in medicinal plants have the potential as antibacterial agents. Thus, endophytic bacteria in the medicinal plant, clove (Syzygium aromaticum), allow it to produce the same bioactive compounds as those contained in its host. This study aimed to analyze the antibacterial activity of five endophytic bacterial isolates from clove leaves. Antibacterial activity was evaluated using disc diffusion assay. Based on the results, isolate Niallia nealsonii DCL1 have strong antibacterial activity against Escherichia coli ATCC 8739, while Bacillus paranthracis DCL3 isolate has intermediate antibacterial activity against Pseudomonas aeruginosa ATCC 15442. In addition, extracellular metabolites of these two isolates of DCL1 and DCL3 which harvested at stationary phase showed antibacterial activity against and E. coli ATCC 8739 and P. aeruginosa ATCC 15442. These data indicate that the potential antibacterial compounds from DCL1 and DCL3 are considered extracellular secondary metabolites. Further analysis on the mode of antibacterial action should be carried out for further medical application.
Co-Authors Ahmad Randy Diyah Tri Utami Gian Primahana Karina Eku Dwinanda Gunawan Muhammad Eka Prastya Nadya Nabila Qurani NISA RACHMANIA MUBARIK Rika Indri Astuti