Article Per Year (5 Year)
p-Index From 2021 - 2026
1.945
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Sumberdaya Hayati Quagga: Jurnal Pendidikan dan Biologi Biodiversitas Journal of Biological Diversity
Jepri Agung Priyanto
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Immunology & microbiology

Published : 10 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 10 Documents
Search

 1 
An Evaluasi Pelaksanaan Workshop Daring Penerapan Ilmu Biologi di SMA Plus Al Wahid Tasikmalaya Nisa Rachmania Mubarik; Mira Dewi; Jepri Agung Priyanto
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 8 No. 1 (2022): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.8.1.93-100

Abstract

The workshop on the application of Biology online (on a network) using the internet during the Covid-19 pandemic has been held at SMA Plus Al Wahid, Tasikmalaya Regency. This activity is in the context of a community service program entitled IPB lecturers to serve. The objectives of this activity are: 1) To describe the implementation of an online explanation workshop program for high school level Biology learning, especially those related to viruses and body immunity; 2) Explaining the level of understanding of the workshop participants before and after the presentation of the material; and 3) Explaining the results of the evaluation of participants' satisfaction with the delivery of workshop materials. The workshop was held on September 4, 2020 online using the Zoom application platform. The number of participants who took part in the workshop was 43 people, consisting of students in grades X, XI, and XII in the natural sciences, teachers, and the general public (non-teacher high school employees) from SMA Plus Al Wahid. Only 16 student participants who filled out the pre-test and post-test were used to evaluate this activity. Evaluation of the results of the pre-test and post-test using a one-group pre-test-post-test design using the N gain method or normalized gain analysis. The results of the workshop showed an increase in participants' understanding of the material on the application of Biology by 30.6% and participants showed a satisfaction level of 76.25%.
Paederia foetida Linn Leaves-Derived Extract Showed Antioxidant and Cytotoxic Properties Against Breast Carcinoma Cell Jepri Agung Priyanto; Muhammad Eka Prastya; Gian Primahana; Ahmad Randy; Diyah Tri Utami
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.271-280

Abstract

Paederia foetida Linn. is a rich source of active compounds, yet its antioxidant and cytotoxic properties remain poorly studied. This current research aimed to investigate the chemical profiles of a crude extract derived from P. foetida leaves, its fractionated substances, and their antioxidant and cytotoxic properties on MCF-7 cells, a breast carcinoma cell. Six fractionated substances have been separated in thin layer chromatography (TLC) plate. Five fractions showed antioxidant properties, as indicated by the formation of the yellowish band on the TLC plate after spraying with the DPPH reagent. Furthermore, crude extract of P. foetida leaves and its fractions are also characterized by antioxidant properties against 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. F1 fraction exhibited the most potent antioxidant activity with IC50 values of 10.94±2.67 and 50.04±0.48 µg/ml, respectively, toward DPPH and ABTS radicals. Crude extract and all six fractions also varied in their cytotoxic properties against MCF-7 cells, with IC50 values ranging from 410.24±0.30 to 831.57±6.91 µg/ml. F3 fractions showed the strongest cytotoxicity (IC50 value: 410.24±0.30 µg/ml). Methanolic extract and active plant leaf fractions contained flavonoid and phenolic compounds, as measured by aluminum chloride and Folin-Ciocalteu reagent, respectively. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis showed that phenol, flavonoid, alkaloid, limonoid, and steroid were the major compound found in the crude extract and fractions. In conclusion, the present study proved that crude extract of P. foetida leaves and its fractionated substances were potential as antioxidant and cytotoxic agents.
Seleksi dan Karakterisasi Bakteri Halotoleran Penghasil Enzim Amilase Dari Produk Fermentasi Ikan-Inasua: Selection and Characterization of Halotolerant Bacteria that produce Amylase Enzyme from Fish-Inasua Fermented Food Karina Eku Dwinanda Gunawan; Jepri Agung Priyanto; Nisa Rachmania Mubarik
Jurnal Sumberdaya Hayati Vol. 9 No. 3 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.3.96-103

Abstract

Fish-inasua fermented food is a potential source to obtain halotolerant bacteria-producing amylase. Previously, four bacterial isolates such as IG12, IG6, IG66, and IG31, isolated from this food, have been reported to be able to produce protease and lipase enzyme, but their potential as amylase enzyme producers have not been studied. This study aims to characterize and test the potential of halotolerant bacteria isolated from this food in producing amylase enzymes. Based on Gram staining, four bacterial isolates were Gram-positive. The qualitative test of amylase on NA medium with the addition of 1% starch showed four bacteria were able to produce amylase in NaCl (0 to 5%)-containing medium. IG66 isolate was the most potential isolate because it had the highest enzyme activity in a medium with 5% NaCl concentration, which was 0.095 U/ml. The amylase activity was produced maximum at the 8th hour, which is the early stationary phase. Its maximum activity in the 5% NaCl-containing medium was at the 10th hour. In addition, amylase produced by IG66 isolate reached optimum activity at a temperature of 50°C and pH 7. This study concluded that IG66 isolate was the most potential halotolerant isolate that could be developed as an amylase-producing agent.
Antibacterial Activity of Endophytic Bacterial Isolates from Kaempferia galanga Leaves Rafifa Ridha; Dira Hefni; Zetryana Puteri Tachrim; Gian Primahana; Jepri Agung Priyanto; Linosefa Linosefa; Adrial Adrial; Endrinaldi Endrinaldi; Muhammad Eka Prastya
Jurnal Kimia Sains dan Aplikasi Vol 27, No 11 (2024): Volume 27 Issue 11 Year 2024
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.27.11.538-548

Abstract

Indonesia has a high biodiversity, which can be relied upon as a potential source of medicinal materials. One of the medicinal plants in Indonesia is Kaempferia galanga, which demonstrates various pharmacological properties, including antibacterial, anti-inflammatory, antioxidant, anticancer, and antiangiogenic effects. However, directly extracting active compounds from plants requires a considerable amount of biomass. To address this challenge, utilizing endophytic bacteria associated with these plants presents a promising alternative. Consequently, the antibacterial activity of endophytic bacterial isolates from K. galanga leaves needs to be investigated. This study is an experimental laboratory investigation conducted in vitro. Two isolates demonstrated antibacterial activity: isolate code DR4 inhibited the growth of Escherichia coli (3 ± 0.5 mm), while isolate DR10 inhibited the growth of four bacteria: Bacillus subtilis (5 ± 0 mm), Staphylococcus aureus (1.5 ± 0.5 mm), Escherichia coli (1 ± 0 mm), and Pseudomonas aeruginosa (1 ± 0 mm). Disc diffusion tests using ethyl acetate extracts of isolate DR10 showed the highest antibacterial activity at a concentration of 10,000 ppm. The Minimum Inhibitory Concentration (MIC) was determined to be 156.2 ppm against B. subtilis, while the Minimum Bactericidal Concentration (MBC) was >625 ppm. Molecular identification showed that isolate DR 10 had 100% similarity to Bacillus sp. TS8. The active compound suspected to have antibacterial properties is pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, with the highest abundance.
Antibacterial Activity of Clove-derived Endophytic Bacteria of Niallia nealsonii DCL1 and Bacillus paranthracis DCL3 Nadya Nabila Qurani; Jepri Agung Priyanto; Rika Indri Astuti
Quagga: Jurnal Pendidikan dan Biologi Vol 18 No 1 (2026): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v18i1.470

Abstract

Pathogenic bacterial infection is still a major health problem that causes death. Endophytic bacteria in medicinal plants have the potential as antibacterial agents. Thus, endophytic bacteria in the medicinal plant, clove (Syzygium aromaticum), allow it to produce the same bioactive compounds as those contained in its host. This study aimed to analyze the antibacterial activity of five endophytic bacterial isolates from clove leaves. Antibacterial activity was evaluated using disc diffusion assay. Based on the results, isolate Niallia nealsonii DCL1 have strong antibacterial activity against Escherichia coli ATCC 8739, while Bacillus paranthracis DCL3 isolate has intermediate antibacterial activity against Pseudomonas aeruginosa ATCC 15442. In addition, extracellular metabolites of these two isolates of DCL1 and DCL3 which harvested at stationary phase showed antibacterial activity against and E. coli ATCC 8739 and P. aeruginosa ATCC 15442. These data indicate that the potential antibacterial compounds from DCL1 and DCL3 are considered extracellular secondary metabolites. Further analysis on the mode of antibacterial action should be carried out for further medical application.
Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria MAULIN INGGRAINI; SITI NURFAJRIAH; JEPRI AGUNG PRIYANTO; Noor Andryan Ilsan
Biodiversitas Journal of Biological Diversity Vol. 22 No. 2 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220206

Abstract

Abstract. Inggraini M, Nurfajriah S, Priyanto JA, Ilsan NA. 2021. Antimicrobial susceptibility and molecular species identification of clinical carbapenem-resistant bacteria. Biodiversitas 22: 555-562. Antibiotic is the first option treatment for infectious diseases both in human and animal. However, the excessive usage and misuse of antibiotics have driven antibacterial resistances worldwide and the increasing case of antibiotic resistance leads to limited options for treatment. This study aimed to observe antimicrobial susceptibility and molecular identification of carbapenem-resistant human clinical bacteria. A total of nine isolates in this study were collected in 2020 from a teaching hospital in Indonesia. All isolates were originated from various human clinical specimens, including urine, blood, pus, and sputum. Identification using 16s rRNA-based showed that these isolates were closely related to Klebsiella pneumoniae (1/9), A. baumannii (5/9), Escherichia coli (2/9), and Lysinibacillus fusiformis (1/9). According to minimum inhibitory concentration using Vitek Automated Machine, four isolates of multi-drug resistant (MDR) bacteria were found. In contrast, five of them were categorized as extensively-drug resistant (XDR). Interestingly, all of the XDR isolates belonged to A. baumannii. These isolates were resistant to at least seven different antimicrobial classes. A comparison of partial 16s rRNA showed two E. coli had similar variance. While in A. baumannii isolates, we found one of five isolates had a different variance sequence, which suggests different clonality among this species. This study gives an insight into the prevalence of carbapenem-resistant bacteria with XDR criteria in Indonesia.
Screening of chitinase-producing rhizosphere actinomycetes and their genetic diversity Aris Tri Wahyudi; NAUFAL GHAZI FITHRIANSYAH; MUHAMMAD FAIZ AMRI; JEPRI AGUNG PRIYANTO; ABDJAD ASIH NAWANGSIH
Biodiversitas Journal of Biological Diversity Vol. 22 No. 10 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221008

Abstract

Abstract. Wahyudi AT, Fitriansyah NG, Amri MF, Priyanto JA, Nawangsih AA. 2021. Screening of chitinase-producing rhizosphere actinomycetes and their genetic diversity. Biodiversitas 22: 4186-4192. Soil microbial community, especially rhizosphere actinomycetes, plays an important role in protecting plants from phytopathogenic fungi by producing various agroactive compounds, including mycolytic enzymes particularly chitinase. The objective of this study was to investigate chitinase activity and analyze the diversity of family 19 chitinase genes of actinomycetes isolated from maize and soybean rhizosphere. Of fivety actinimycetes, Seventeen isolates exhibited chitinolytic activities and formed a hydrolytic zone around the colony with chitinolytic index ranging from 0.49±0.01 to 2.15±0.69, qualitatively tested in 0.3% chitin agar medium. Six selected isolates (ARJ 36, ARJ 81, ARJ 15, ARK 17, ARK 143, ARK 103) showed chitinase activities ranging from 0.157±0.04 to 0.440±0.09 U/mL, based on chitinolytic index. The production of chitinase was confirmed by the presence of family 19 chitinase encoding genes from Streptomyces sp. Some conserved regions and essential amino acid residues were also detected. This study suggests that chitinolytic actinomycetes isolated from maize and soybean rhizosphere can be studied further as biological control candidates for controlling phytopathogenic fungi.
Short Communication: The potency of lytic bacteriophage isolated from various environments to control the growth of Citrobacter braakii causing urinary tract infection: Bacteriolytic activity of bacteriophage against Citrobacter braakii SHEILAHRUSI SHEILAHRUSI; JEPRI AGUNG PRIYANTO; HENI RISMIYATI; IMAN RUSMANA; SRI BUDIARTI
Biodiversitas Journal of Biological Diversity Vol. 22 No. 12 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d221239

Abstract

Abstract. Sheilahrusi, Priyanto JA, Rismiyati H, Rusmana I, Budiarti S. 2021. Short Communication: The potency of lytic bacteriophage isolated from various environments to control the growth of Citrobacter braakii causing urinary tract infection. Biodiversitas 22: 5550-5554. Citrobacter braakii is one of the pathogenic bacteria causing urinary tract infection (UTI) in humans. Bacteriophages that are specifically infecting C. braakii could be the alternatives to combat antibiotics resistance cases of this bacterium. This study aimed to isolate lytic phages from various environmental samples (tofu factory wastewater, sewage water, rice field water, fishpond water, cattle farm wastewater, and goat farm wastewater), and to analyze their effectivity to reduce UTI-causing C. braakii population in vitro. Results exhibited that phages targeting this bacterium were found in goat and cattle farm wastewater and not present in other samples. Two phages, namely FC1 and FC2, had different plaque morphology characteristics. The number of phages in cattle farm wastewater was 2.8 × 105 PFU/mL and 1.32 × 104 PFU/mL in goat farm wastewater. It was observed that the phages found in these environments also indicate the presence of their host. After 8 h incubation, FC1 and FC2 phage reduced UTI-causing C. braaakii population in vitro from 0.23×108 CFU/mL to 0.03×108 CFU/mL and 0.02×108 CFU/mL, respectively. The phages isolated from these two samples can be further developed for the treatment of UTI caused by C. braakii and goat and cattle farm wastewater treatment to prevent contaminating other areas.
Glucanase activity produced by rhizospheric Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and the analysis of their encoding genes DEMMA ZILBA BUDIMAN; WIDYA ESTI PURWANINGTYAS; JEPRI AGUNG PRIYANTO; IVAN PERMANA PUTRA; ABDJAD ASIH NAWANGSIH; ARIS TRI WAHYUDI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241065

Abstract

Abstract. Budiman DZ, Purwaningtyas WE, Priyanto JA, Putra IP, Nawangsih AA, Wahyudi AT. 2023. Glucanase activity produced by rhizospheric Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and the analysis of their encoding genes. Biodiversitas 24: 5831-5837. The enzyme ?-1,3-glucanase is capable of breaking down the glucan components in the cell walls of phytopathogenic fungi. Streptomyces spp. isolated from the maize rhizosphere, are believed to produce enzymes with glucanolytic activity, making them promising candidates for use as a biological control agent against these fungi. This work aims to quantitatively analyze the enzymatic activity of glucanase in Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and identify their encoding genes (bglS) and the three-dimensional modeling of their protein structures. The dinitrosalicylic acid (DNS) method quantitively assays the glucanase enzyme. The TA-Cloning of the bglS gene was performed using pGEM-T Easy Plasmid Vector, and the three-dimensional protein structure was constructed using the I-TASSER program. According to the findings, both Streptomyces isolates exhibited glucanolytic activity. The glucanase enzyme activities of S. tritolerans ARJ 32 and S. collinus ARJ 38 peaked at eight days of incubation, with values of 31.116 U/mg and 41.599 U/mg, respectively. The partial bglS gene was present in both Streptomyces and identified as endo-?-1,3-glucanase from the glycoside hydrolase family 16 (GH 16). The deduced partial amino acid sequences were aligned and showed some highly conserved residue in the catalytic domain of GH 16. The three-dimensional structural model built from the partial bglS amino acid sequences displayed high-quality parameters and overlapped with the protein model of partial endo-?-1,3-glucanase from Nocardiopsis sp. F96 (2HYK). These preliminary results suggest that the two Streptomyces isolates had the potential to be used as glucanase enzyme producers.
Genome mining, metabolite fractionation, and evaluation of antibiofilm activity of endophytic Priestia megaterium AJ5 against methicillin-resistant Staphylococcus aureus JEPRI AGUNG PRIYANTO; GENIA SOTYA SINARAWADI; MUHAMMAD EKA PRASTYA; ORHINTA MUTIARA JATI; NISA RACHMANIA; ZETRYANA PUTERI TACHRIM
Biodiversitas Journal of Biological Diversity Vol. 26 No. 6 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260634

Abstract

Abstract. Priyanto JA, Sinarawadi GS, Prastya ME, Jati OM, Rachmania N, Tachrim ZP. 2025. Genome mining, metabolite fractionation, and evaluation of antibiofilm activity of endophytic Priestia megaterium AJ5 against Methicillin-resistant Staphylococcus aureus. Biodiversitas 26: 2916-2925. Endophytic bacteria, particularly Priestia megaterium, are a source of secondary metabolites that are valuable for medicinal purposes. Therefore, this study aimed to mine the Secondary Metabolite Gene Clusters (SMGCs) within the complete genome of AJ5 strain, fractionate its secondary metabolites, and evaluate its antibiofilm activity against Methicillin-Resistant Staphylococcus aureus (MRSA). The bacterium had a complete genome length of 5,750,510 bp and was closely related to P. megaterium NCTC10342, with an Average Nucleotide Identity (ANI) of 98.37%. AntiSMASH analysis showed that the bacterium possessed 5 types of SMGCs, including Non-Ribosomal Peptide Synthetase (NRPS)-Independent (NI) siderophores, 3 types of terpenes, and type III Polyketide Synthase (PKS). In addition, 2 selected fractions obtained from thin-layer chromatography and column chromatography procedures exhibited strong anti-MRSA activity. F9 and F12 fractions had Minimum Inhibitory Concentrations (MIC) of 117.5 ?g/mL and 180 ?g/mL, respectively. Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) analysis indicated that these 2 fractions contained nigakilactone H, tetratriacontanamine, 2-(p-Anisyl)-5-methyl-1-hexene, and other unidentified compounds. The compounds were also capable of suppressing biofilm formation of MRSA by up to 81.54%±1.80 for the F9 and 77.01%±1.96 for F12 fraction after treatment with 1×MIC. All selected fractions also exhibited eradication activity against established MRSA biofilms up to 79.23±1.96%. In conclusion, this study showed the SMGCs profile and their metabolite products found within the genome of AJ5 strain, as well as its antibiofilm potential against MRSA. The thoroughness of our research process and the validity of our findings suggest that genome-based screening and in vitro assays could significantly accelerate the development of novel anti-MRSA agents.
Co-Authors Abdjad Asih Nawangsih Adrial Adrial Ahmad Randy Aris Tri Wahyudi DEMMA ZILBA BUDIMAN Dira Hefni Diyah Tri Utami Endrinaldi GENIA SOTYA SINARAWADI Gian Primahana Gian Primahana HENI RISMIYATI Iman Rusmana Ivan Permana Putra Karina Eku Dwinanda Gunawan Linosefa Linosefa MAULIN INGGRAINI MUHAMMAD EKA PRASTYA Muhammad Eka Prastya Muhammad Eka Prastya MUHAMMAD FAIZ AMRI Nadya Nabila Qurani NAUFAL GHAZI FITHRIANSYAH NISA RACHMANIA MUBARIK Noor Andryan Ilsan ORHINTA MUTIARA JATI Rafifa Ridha Rika Indri Astuti SHEILAHRUSI SHEILAHRUSI SITI NURFAJRIAH SRI BUDIARTI WIDYA ESTI PURWANINGTYAS ZETRYANA PUTERI TACHRIM Zetryana Puteri Tachrim