Article Per Year (5 Year)
p-Index From 2021 - 2026
1.116
P-Index
This Author published in this journals
All Journal Indonesian Journal of Cancer Chemoprevention Biomedika Pharmacon BIOMA : Jurnal Biologi Makassar Journal of Health (JoH) Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Analis Medika Biosains (JAMBS) Medika Kartika : Jurnal Kedokteran dan Kesehatan Borneo Journal of Biology Education (BJBE)
Diani Mentari
Faculty Of Biology, Universitas Jenderal Soedirman, Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
EVALUASI KUALITAS THROMBOCYTE CONCENTRATE SELAMA MASA SIMPAN LIMA HARI BERDASARKAN VARIASI USIA PENDONOR Bria, Theodosia Claranty Nahak; Mentari, Diani; Sepvianti, Wiwit; Pebriani, Relita; Tirtana, Arif; Nurpratami, Diah
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 7 No 2 (2024): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Thrombocyte Concentrate (TC) merupakan komponen darah yang digunakan untuk meningkatkan jumlah trombosit pada pasien yang mengalami trombositopenia. Komponen darah TC secara in vitro dapat disimpan selama 5 hari pada platelet agitator. Kontrol kualitas merupakan faktor yang penting untuk menjaga produk TC tetap dalam kondisi baik. Selama penyimpanan TC dapat mengalami berbagai perubahan kualitas. Usia merupakan faktor yang dapat memengaruhi kualitas darah itu sendiri. Semakin bertambahnya usia seseorang maka metabolisme tubuh akan ikut menurun. Hal ini dapat berpengaruh terhadap produksi trombosit. Saat ini, penelitian evaluasi kualitas TC pendonor dengan perbedaan usia belum pernah diteliti. Sampel TC diambil dari pendonor laki-laki berusia ≤ 30 dan > 30 tahun yang telah lolos seleksi donor, selanjutnya TC disimpan selama 5 hari. Sampel dianalisis pada hari ke 1, 3, 5 dengan beberapa parameter yaitu jumlah trombosit yang diukur menggunakan hemotology analyzer, serta swirling dan tingkat kekeruhan yang diukur secara visual. Pengamatan TC secara visual pada hari ke-5 menunjukkan swirling skor 2 dan nampak terjadi kekeruhan pada sampel TC pendonor ≤ 30 tahun. Selain itu jumlah TC yaitu 9 x 109 sel/unit. Pada pendonor usia > 30 tahun jauh lebih baik, dengan swirling skor 3 dan tidak adanya kekeruhan dan jumlah TC yaitu 26 x 109 sel/unit. Sampel darah TC dari pedonor usia > 30 tahun memiliki kualitas yang jauh lebih baik dibandingkan dengan pendonor ≤ 30 tahun. Maka dapat disimpulkan bahwa usia seseorang tidak dapat menentukan kualitas komponen darah TC. Kata kunci: kontrol kualitas, swirling, thrombocyte concentrate, trombosit, usiaDOI : 10.35990/mk.v7n2.p112-123
Human Platelet Lysate (HPL) as an Alternative Media Propagation of T47D Cells Line Mentari, Diani; Pebrina, Relita; Nurpratami, Diah
Indonesian Journal of Cancer Chemoprevention Vol 11, No 1 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss1pp36-45

Abstract

Fetal bovine serum (FBS) is a gold standard as a supplement to cell and tissue culture media. This is due to a large number of Growth Factor (GF) contained in FBS. However, the use of FBS is at risk of transferring endotoxins, prions, bacteria and viruses from animals to humans, so it is risky to be used on cell therapy. Human Platelet Lysate (HPL) is a medium that can be developed as an alternative cell growth medium. The advantage of HPL is that it does not contain aggregate platelets so it does not cause the cells to clot. This condition causes HPL to be used as a substitute medium replacing FBS for cell propagation. The use of HPL for cell propagation has been widely reported. However, the use of HPL in cancer cells has not been found. Thus, this study aims to see the effectiveness of HPL as a T47D cell culture medium. The study began with donor selection with criteria for the male sex, the blood type O, the age ≤35 years. Furthermore, the Platelet Concentrate (PC) was processed into HPL then measured pH, total protein and albumin levels. The cell viability was measured using the MTT assay to determine the ability of cell proliferation when propagation using HPL. The doubling time test was carried out as in the cell proliferation test. However, the incubation was carried out for 24 h, 48 h and 72 h and the HPL concentration used was 5%. The result shows that HPL 10% and 20% ability to increase proliferation better than the FBS 10%. HPL with a 5% concentration ability to shortens the doubling time than FBS 10% (doubling time is less than 19.94 h). It this study, cell proliferation is influenced by the pH of HPL and total protein but not by the amount albumin.Keywords: Human Platelet Lysate, Proliferation, T47D cell line, total protein, albumin.
Impact of Donor Age on Human Platelet Lysate Quality and its Consequential Effects on HeLa Cell Growth in the Presence of Anti-Cancer Compounds Mentari, Diani; Wijayanti, Gratiana Ekaningsih; Suhesti, Tuti Sri; Muhammad, Katon; Ritchie, Ni Ken; Nurpratami, Diah
Indonesian Journal of Cancer Chemoprevention Vol 14, No 3 (2023)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev14iss3pp189-198

Abstract

An integral aspect of anticancer experimentation involves delineating the optimal dosage of the test compound to ascertain its efficacy in targeting malignant cells. Numerous variables may influence a compound's cytotoxicity, among which is the choice of cell culture medium. Within in vitro settings, supplementary mediums are employed to foster cellular proliferation. Platelet lysate (PL) serves as a growth supplement, presenting an alternative to fetal bovine serum (FBS), primarily due to its incorporation of growth factors such as platelet-derived growth factor (PDGF), a component absents in FBS. The integrity of PL may be subject to various factors, including the age of the donor. This study sought to evaluate the impact of donor age on PL quality. Furthermore, it aimed to discern whether PL derived from platelet concentrate (PC) blood components of different age cohorts influences the IC50 value in anticancer compound assessment. Expired PCs were utilized, subsequently classified into age categories: ≤30 years, >30 years, and a combination of ages. PL analysis encompassed parameters such as pH, blood profile, protein, glucose, and cholesterol levels. The investigation scrutinized the influence of PL quality, as a cellular growth supplement, on the anticancer compound cisplatin's activity against HeLa cells. Findings indicate that donor age influenced the IC50 value of cisplatin on HeLa cells. Notably, elevated cholesterol levels and decreased pH in PL from donor ages >30 years were associated with reduced cisplatin toxicity.Keywords: Cisplatin, Donor Age, HeLa, IC50, Platelet Lysate.
Co-Authors Amanu, Aurelya Putri Bria, Theodosia Claranty Nahak Diah Nurpratami Diah Nurpratami Hieronymus Rayi Prasetya Hieronymus Rayi Prasetya, Hieronymus Rayi Jaka Widada JAKA WIDADA Luthfiyyah, Sri Meyta Wulandari Mirtani Naima Muhammad, Katon Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Ni Ken Ritchie, Ni Ken Nurpratami, Diah Pebriani, Relita Pebrina, Relita Rassajati, Syaqina Relita Pebrina Relita Pebrina Relita Pebrina Relita Pebrina Riska Wulansari Sepvianti, Wiwit St. Raihanun Suhadia, Maria Hyasinta Vanesa Putri Syaqina Rassajati Tika, Debek Bini Dita Rahayu Mayang Tirtana, Arif Tri Rini Nuringtyas Tri Wibawa Tri Wibawa Tuti Sri Suhesti Wijayanti, Gratiana Ekaningsih Wulansari, Riska