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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management MEDIA KONSERVASI Jurnal Ilmu Lingkungan Jurnal Penelitian Hutan dan Konservasi Alam Current Biochemistry The Journal of Pure and Applied Chemistry Research Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Journal of Tropical Biodiversity and Biotechnology TREUBIA ZOO INDONESIA Jurnal Penelitian Perikanan Indonesia Scripta Biologica Journal of Tropical Ethnobiology Zoo Indonesia Habitus Aquatica : Journal of Aquatic Resources and Fisheries Management Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik)
Mirza Dikari Kusrini
Department Of Forest Resources Conservation And Ecotourism, Faculty Of Forestry, Bogor Agricultural University, Dramaga Campus, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : Current Biochemistry

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Phylogenetic Analysis of Cytochrome Oxidase I from Buduk Toads Duttaphrynus melanostictus and Phrynoidis asper from Bogor Muhammad Dailami; I Made Artika; Mirza Dikari Kusrini; Dodi Safari
Current Biochemistry Vol. 3 No. 2 (2016)
Publisher : IPB University

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Abstract

Indonesia have high diversity of Amphibians. Amphibians have an important role in ecosystem and produce many bioactive peptides. However, the genetic information of amphibians from Indonesia is very limited, especially Duttaphrynus melanostictus and Phrynoidis asper. The aims of this study are to determine the nucleotide sequence of cytochrome oxidase I (COI) from D. melanostictus and P. asper, to analyze their genetic diversity and their phylogenetic relationship. A total 668 base pairs of COI gene fragment were successfully amplified and their nucleotide sequence determined. P. asper (5 haplotypes) samples group have high haplotype diversity compared to D. melanostictus (1 haplotype). The results of Basic Local Alignment Search Tools (BLAST) to the NCBI and BOLD database, showed 99 % - 100 % identity to sequence of D. melanostictus. For the sequence of P. asper showed 99.23 % identity to sequence P. asper in BOLD database. There was no sequence of COI gene of P. asper in NCBI database. Genetic relationship among species in family Bufonidae, indicated that D. melanostictus has closer relation to P. asper than to another species, inspite of their pharapyletic characteristic. For intern species relationship of D. melanostictus, the data showed that D. melanostictus from Bogor have closer relationship to D. melanostictus from India than D. melanostictus from China.
Co-Authors Adininggar Ul-Hasanah Agus Priyono Kartono Aini, Yusratul Andayani, Noviar Andhika Prima Yudha Ani Mardiasturi Ani Mardiastuti Anika Putri Aprilianti, Risma Aria Nusantara Arief Tajalli Ariefiandy, Achmad Arzyana Sunkar Bakhtiar Aji Boby Darmawan Burhanuddin Masy'ud Burhanuddin Masy'ud Burhanuddin Masy'ud Burhanuddin Masy’ud Darmawan, Boby DEDE AULIA RAHMAN Dian Oktaviani Dodi Safari Duto Nugroho Dwi Fania Endang Hernawan Erna Suzanna Eterna Firliansyah Evy Arida Fata Habiburrahman Faz Fata Habiburrahman Faz Fauzia, Alienda M Frayoga, Yunus Arif Fredinan Yulianda Guntoro, Joko Hadi S Alikodra Hadi S. Alikodra Hanny Herzegovina Harnum Nurazizah I MADE ARTIKA Ida Ayu Ari Janiawati Ira Khoerunisa Jakaria, Mohamad Kaniwa Berliani Kaniwa Berliani Kaniwa Berliani Kennedi, Umar Fhadli Kristina Nainggolan Larasati, Firda LILIK BUDIPRASETYO Loar, Loise Liberta Luna Raftika Khairunnisa Luthfia Nuraini Rahman Maddock, Simon T Mahmud, Arief Mohamad Jakaria Mohammad Ali Ridha Mohammad Mukhlis Kamal Muhamad Kurniawan, Muhamad Muhammad Dailami, Muhammad MUHAMMAD IRFANSYAH LUBIS Muhammad Irfansyah Lubis Nancy Karraker Noor Farikhah Haneda Novi Tri Ayuningrum Nusantara, Muhammad Galih Gurenda Prabowo, Oktavianus Jekki Pranata, I Putu Agus Widi Purwantono Purwantono Rahman, Luthfia Nuraini Rahmat Abdiansyah Ramadhan, Tubagus Muhamad Daffa Ramdani Manurung Ramdani Manurung Resti Meilani Rodrigo Ibrrandi Vazquez Sambas Basuni Sari, Fitri Eka Sawaki, Morgan Wayne Luther Setiawan, Rezky Agust Sigit Purwanto Suci Ninda Utari Tiara, Alma Vidya Elza Loriza Wempy Endarwin Yanto, A Viktor Yeni Aryati Mulyani Yudi Setiawan Zainudin