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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan jurnal Ilmu Pertanian (Agricultural Science) Jurnal Penelitian Tanaman Industri (Littri) Agrokompleks
SEDYO HARTONO
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Jurnal Perlindungan Tanaman Indonesia

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Karakterisasi Virus Penyebab Penyakit Belang pada Tanaman Lada (Piper nigrum L.) Trisnani Alif; Sedyo Hartono; Sri Sulandari
Jurnal Perlindungan Tanaman Indonesia Vol 22, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.30354

Abstract

Mottle disease is an important disease in pepper plants caused by Piper yellow mottle virus (PYMoV). This study aims to determine the characterization of PYMoV biologically and molecularly. The pepper plant samples were obtained from pepper farmland in Kleben, Putat (Yogyakarta), and Air Buluh (Bangka). Virus particles are measured by electron microscopy. Virus transmission studies include mechanical transmission, vector, cuttings, grafting, and seeds. The molecular detection was done by using Polymerase chain reaction (PCR) method with PYMoV-F and PYMoV-R specific primers. The result, virus particles were found to be ± 30×130 nm in shape. Virus transmission studies indicate that PYMoV can be transmitted by Ferrisia virgata vectors, cuttings, grafts and seeds but cannot be transmitted through mechanical inoculation. Molecular test results showed that samples of Kleben, Putat and Air Buluh pepper plants were positively detected to contain PYMoV and amplified at 400 bp. The result of nucleotide base sequence analysis showed the isolates of Putat and Air Buluh had the highest homology with PYMoV of India 2 about 95% while Kleben isolate had 96% homology with PYMoV of India 1. IntisariPenyakit belang merupakan salah satu penyakit penting pada tanaman lada yang disebabkan oleh Piper yellow mottle virus (PYMoV). Penelitian ini bertujuan untuk mengetahui karakterisasi PYMoV secara biologi dan molekuler. Sampel tanaman lada diperoleh dari lahan petani lada di Desa Kleben, Putat (Yogyakarta), dan Air Buluh (Bangka). Partikel virus diukur dengan mikroskop elektron. Kajian penularan virus meliputi penularan mekanik, vektor, stek, penyambungan, dan biji. Deteksi secara molekuler dengan metode Polymerase chain reaction (PCR) dengan pasangan primer spesifik PYMoV-F dan PYMoV-R. Partikel virus yang ditemukan berukuran ± 30×130 nm berbentuk batang. Kajian penularan virus menunjukkan bahwa PYMoV dapat ditularkan melalui vektor Ferrisia virgata, stek, penyambungan dan biji namun tidak dapat ditularkan melalui inokulasi mekanik. Hasil uji molekuler menunjukkan bahwa sampel tanaman lada Kleben, Putat dan Air Buluh positif terdeteksi PYMoV dan teramplifikasi pada 400 bp. Hasil analisis sekuen basa nukleotida menunjukkan isolat Putat dan Air Buluh memiliki homologi tertinggi dengan PYMoV India 2 sekitar 95% sedangkan isolat Kleben memiliki homologi 96% dengan PYMoV India 1.
Responses of Tomatoes Grafting Using Variation of Rootstock against Virus Infection and Tomato Yields Tri Retno Widyastuti; Sri Sulandari; Sedyo Hartono; Triwidodo Arwiyanto
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.31056

Abstract

Grafting methods on tomato have been done to reduce the infection rate of various pathogens. Begomovirus and Crinivirus are important viruses in tomato plants. The research aimed to determine the resistance response of tomato plants to viral infection, and tomato production. Field research was conducted in Harjobinangun, Pakem, Sleman, Yogyakarta in the endemic area of the viral diseases transmitted by Bemisia tabaci. This experiment used a Completely Randomized Design non-factorial with “Servo” as scion and “Amelia”, “H-7996”, “Mawar” as rootstock. The disease development, presence of viral diseases, and tomato yields were observed. PCR detection using Krusty & Hommr primer successfully amplified Begomovirus DNA bands with an approximate size of 580 bp in tomato plant with interveinal chlorosis, curling, thick, rigid, and stunt symptoms. Chlorotic spots and yellowing symptoms successfully amplified using ToCV-CF/ToCV-CR specific primer for the amplification of Tomato chlorosis virus with DNA band approximately size of 360 bp, whereas using TICV-CF/TICV-CR specific primer could not amplify the virus cDNA. The leaves roll upward with purple interveinal symptoms that were not infected by both viruses. Both viral infections affected the quality of the fruit which indicated by a higher number of abnormal fruits. “Servo” grafted onto “Amelia” and non-grafted Servo were tolerant to viral infection, “Servo” grafted onto “H-7996” or to “Mawar variety were susceptible to viral infection, self-grafted Servo were very susceptible to viral infection. 
Molecular Identification of Begomovirus Infecting Angled Luffa Alvina Clara Giovanni; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 2 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.31073

Abstract

Begomovirus was reported as one of the most aggressive and destructive viruses on several commercial crops, including cucurbits in Indonesia. Plants that infected with Begomovirus show the mosaic symptom on the leaves, change in leaf shape, stunts, change in color and shape of fruit. It was recently observed in cultivated angled luffa [Luffa acutangula (L.) Roxb] around Yogyakarta and Central Java. The aim of this research was to identify the virus by using Polymerase chain reaction (PCR). The result of Begomovirus amplification from the total DNA samples amplification using primer Krusty-Homer showed that DNA of Begomovirus from angled luffa was amplified at ~580bp. The DNA sequencing of angled luffa’s leaf isolate GD1 had 97.8% homology with SCLV-China isolate MC1. However, amplification of DNA seed samples using the same primer showed negative result. It was concluded that Begomovirus was not a seed borne virus. This is the first molecular report on the occurence of Begomovirus in angled luffa in Yogyakarta.
Detection and Analysis of Protein Profile on Rice Infected by Stunting Virus with Different Severity on Ciherang and Situ Bagendit Varieties Selvi Helina; Sri Sulandari; Sedyo Hartono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 1 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.36549

Abstract

Rice stunt virus is one of the limiting factors in the decline of rice production in Indonesia. The virus consists of rice grassy stunt virus (RGSV) and rice ragged stunt virus (RRSV) that is transmitted by brown planthopper (WBC) in a persistent propagative manner. This study aimed to determine the presence of rice stunt virus in Bantul, Yogyakarta through fast detection using RT-PCR. It also aimed to learn the pattern of total protein profile of healthy and infected rice plants by the virus on different severity level in the field. The results showed that rice varieties of Ciherang and Situ Bagendit in Bantul were infected with RGSV and RRSV. They were classified as mild, moderate, severe, and failure in severity level. Homology analysis using BioEdit showed that the nucleotide sequence of RGSV in Bantul isolate had the highest percentage of nucleic acids similarity with Klaten isolate (98.1%). Meanwhile, RRSV of Bantul isolate had the highest percentage of nucleic acids similarity to Philipines isolate (99.5%). Analysis of protein profiles using SDS-PAGE showed a pattern of protein profiles formed on rice infected with the virus at different severity levels which was not found in healthy rice. These proteins presumably were nonstructural p5 and nucleocapsid protein (NCP) of RGSV with a molecular weight of ~22 and 34-35 kDa; and viral spike protein and protein capsid (S8) of RRSV with MW ~39 and ~43 kDa.
Multiplex RT-PCR Assay for Crinivirus Detection Using RNA Prepared from Three Extraction Methods on Tomato Plant Esti Prasetya Ningrum; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.36558

Abstract

Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV) are members of the Crinivirus genus that induces yellowing symptoms in tomato plants. Detection of both viruses is generally carried out singly, thus it is necessary to develop a fast, accurate and efficient detection method to detect multiple viruses simultaneously in an effort to determine the suitable disease management strategies. This study was aimed to detect both viruses using the multiplex RT-PCR method and evaluate three methods of total RNA preparation used from tomato plants as RT-PCR templates. The methods evaluated were simple direct tube (SDT), simple dsRNA, and commercial kit (RNeasy Plant Mini Kit) as a comparison. The total source of RNA came from Crinivirus symptomatic tomato leaves from Kopeng, and Ketep (Central Java); Pakem (Yogyakarta); Malang (East Java); and Bogor (West Java). Single RT-PCR and multiplex RT-PCR using specific primers CPd I/CPd II and ToCV CF/ToCV CR with DNA band targets of 760 bp and 360 bp. The SDT and dsRNA methods have been successful in obtaining total RNA and viral RNA from tomato leaf samples. Total RNA RT-PCR with simple SDT and dsRNA methods followed by multiplex RT-PCR produces specific DNA band intensities that are comparable to Kit. RNA preparation with SDT and simple dsRNA methods is a simple, fast, easy and affordable method in providing templates for RT-PCR. Multiplex RT-PCR technique using two pairs of specific primers CPd I/CPd II and ToCV CF/ToCV CR is suitable for simultaneous testing of TICV and ToCV.
Molecular Characterization of Betasatellite Associated with Begomovirus on Ageratum conyzoides in Magelang, Central Java Argawi Kandito; Sedyo Hartono; Sri Sulandari; Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.46579

Abstract

Ageratum conyzoides is one of the common weeds in Indonesia. The presence of weed can decrease plant yield by resource competition and its role as alternative hosts for pests and diseases, especially begomoviruses transmitted by whitefly. The aim of this research is to detect and characterize the begomovirus-beta satellite in A. conyzoides. A. conyzoides showed severe yellowing symptoms were collected from Magelang, Central Java, Indonesia. Total DNA was extracted and analyzed using PCR method for begomovirus and beta satellite. Begomovirus detection was performed with universal primer Krusty-Homer, resulted a 500 bp DNA fragment. Betasatelit detection performed with specific primer β01/β02, resulted a 1300 bp DNA fragment, indicated presence of a beta satellite associated with the begomovirus. The sequence of begomovirus showed 95% similarity with Tomato Leaf Curl Java Virus (ToLCJaV). The sequence of beta satellites showed 85% similarity with Tomato Leaf Curl Betasatellite (ToLCB). Characterization of beta satellite showed three main characters of beta satellite, TAATATTAC stem-loop structure, Adenine-rich region, and 118 amino acids of putative ORF BC1. 
Molecular Detections and Resistance Response of Six Rice Varieties to Tungroviruses from South Sulawesi Saipul Abbas; Sri Sulandari; Sedyo Hartono; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 24, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.47355

Abstract

The suspected rice virus is found in the field, namely the tungrovirus which is transmitted by green leafhoppers (Nephotettix virescens). The study aimed to detect the tungrovirus molecularly and examine the resistance response of six rice varieties from the transmission of tungrovirus samples from South Sulawesi on a greenhouse scale. Based on the results of molecular detection with RTSV PCR of the double infected sample with DNA bands 1115 bp and RTBV of around 430 bp, Sidrap, and Maros samples were infected by 430 bp size RTBV, while Wajo sample was not detected by both viruses. The results of RTBV sequence analysis showed that the grouping of Sidrap was still one group with Maros and Pinrang samples and different from the group of samples from Malaysia, Thailand, and Philippines. While the grouping of RTSV shows that Pinrang samples are still one group with samples from Bali, Subang, and different from those of the Philippines, India, and Malaysia. The results of transmission in the greenhouse on six rice varieties (TN1, Ciherang, Mekongga, Tukad Unda, Inpari 36, Inpari 37) showed different plant resistance responses such as susceptible, moderately resistant, and resistant reactions based on the amount of disease intensity caused. Varieties that are classified as susceptible are TN1 and Ciherang varieties, moderately resistant, namely Mekongga and Tukad Unda varieties, and resistant varieties namely Inpari 36 and Inpari 37 varieties.
Co-Authors Alvina Clara Giovanni Aminatun Munawarti Anak Agung Gde Raka Swastika ANDI KHAERUNI Ani Widiastuti Argawi Kandito Argawi Kandito Argawi Kandito Arman Wijonarko Asmar Hasan Astuti, Suryani Titi Azizah Ridha Ulilalbab Botek, Muhammad Budi Setiadi Daryono BUDI SETIADI DARYONO Cahyo Hertanto Christanti Sumardiyono Christina Retna Handayani Deden Sukmadjaja Dewi Rahmitasari Didit Setiyawan Dini Wahyu Kartika Sari Efendi, Darda Emerensiana - Uge Emerensiana Uge Erna Anastasia Esti Prasetya Ningrum Fariha Wilisiani Fitri Kusumaningrum Gede Suastika GUSNAWATY HS, GUSNAWATY hasdiana, hasdiana Helina, Selvi Heri Widarta Hisein, Siti Anima I Dewa Nyoman Nyana I Nyoman Widiarta Ika Roostika Ika Roostika Ismiyatuningsih Ismiyatuningsih Jun Kobayashi La Ode Santiaji Bande Ma'unah Ambarwati Mery Windarningsih Muhammad Muhsin MUHAMMAD TAUFIK Mustika Ajeng Kartini Putri Pertiwi Nanda Kusumandari Nasrun Nasrun NOOR AIDAWATI Nuri Yusmarlita Nurjanah Nurjanah Praptana, R. Heru Prapto Yudono Prapto Yudono PURNAMA HIDAYAT R. Heru Praptana Rahayu Mallarangeng, Rahayu Rahma Ayu Priani Resti Fajarfika Retno Mastuti Rizko Hadi, Rizko Saipul Abbas Sekar Utami Putri Selvi Helina Serafinah Indriani Siwi Indarti Soesamto Somowiyarjo Somowiyarjo, Susamto Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari Sri Sulandari SRI SULANDARI Sri Sulandari Sri Sulandari Sri Sulandari Subandi Sukamto Sukamto Sumardiyono, Y. B. Suprihanto, Suprihanto susamto - somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Syair, Syair Tomohide Natsuaki Tri Harjaka Tri Joko Tri Joko Tri Maruto Aji Tri Maruto Aji Tri Retno Widyastuti Triharso Triharso Trisnani Alif Triwidodo Arwiyanto Tuty Arisuryanti Ulfa, Nur Isnaini Umi Kulsum Widiarta, I Nyoman Wiwik Endarsih Wuye Ria Andayani Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. Andi Trisyono Y. B. Sumardiyono Y. B. Sumardiyono Y.M.S. Maryudani Yashanti B. Paradisa Yashanti Berlinda Paradisa YB Sumardiyono