Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Ilmiah Mahasiswa Veteriner Jurnal Medika Veterinaria
Nazaruddin Nazaruddin
Laboratorium Patologi Fakultas Kedokteran Hewan Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
PENGARUH PAPARAN TIMBAL (Pb) TERHADAP HISTOPATOLOGIS USUS IKAN NILA (Oreochromis nilloticus) The Effect of Lead (Pb) Exposure to the Histopathology of Nile Tilapia (Oreochromis nilloticus) Intestine Rosmaidar Rosmaidar; Nazaruddin Nazaruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 1 (2017): NOVEMBER - JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.178 KB) | DOI: 10.21157/jim vet..v2i1.5659

Abstract

Penelitian ini bertujuan mengetahui pengaruh paparan timbal (Pb) terhadap histopatologis usus ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 12 ekor dengan kriteria: sehat, bobot badan     15- 18 gram, umur ± 2 bulan, dan jenis kelamin jantan. Penelitian ini merupakan penelitian eksperimental laboratorik dengan desain rancangan acak lengkap yang terdiri dari 4 kelompok perlakuan masing-masing dengan 3 ulangan. Semua kelompok diberikan pakan berupa pelet. Kelompok P0 sebagai kontrol, ikan hanya diberi pakan pelet, P1 diberikan paparan timbal 6,26 mg/L, P2 diberikan paparan timbal 12,53 mg/L, dan P3 diberikan paparan timbal 25,06 mg/L. Perlakuan dilakukan selama 30 hari, dan pengambilan organ usus dilakukan pada hari 31. Sampel usus kemudian diambil dan difiksasi dalam larutan fiksasi dilanjutkan dengan pembuatan sediaan histopatologis dan pewarnaan haematoksilin dan eosin (HE). Pengamatan histopatologis dilakukan dengan mikroskop cahaya biokuler, kemudian untuk pengambilan gambar dengan menggunakan fotomikrograf. Data yang diperoleh dianalisis secara deskriptif.  Hasil pemeriksaan histopatologis usus ditemukan edema, degenerasi lemak, nekrosis, erosi vili usus dan lisis vili usus. Dari hasil ini menunjukkan bahwa semakin tinggi konsentrasi timbal yang diberikan semakin parah kerusakan organ usus yang terjadi. Berdasarkan hasil penelitian dapat disimpulkan bahwa kosentrasi timbal 6,26 mg/l, 12,53 mg/l dan 25,06 mg/l dapat menyebabkan kerusakan jaringan secara histopatologis.The aims of this research was to find out to determine the effect of lead (Pb) exposure to the histopathology of Nile tilapia (Oreochromis nilloticus) intestinal. This study used 12 tilapia fish with criteria: healthy, body weight 15-18 gram, age ± 2 month, and male sex. This study, is a laboratory experiment using complete randomized design with 4 treatments groups, each group were repeated 3 times. Each groups were fed with pellet. P0 as control, fish fed only pellets, P1 is given lead exposure 6,26 mg/L and pellet feed, P2 is given lead exposure 12,53 mg/L and pellet feed and P1 is given lead exposure 25,06 mg/L. Treatment carried out for 30 days, and fish were  euthanized on 31 days. Intestinal samples were collected and fixed in fixation solution followed by histopathology preparation using haematoxylin and eosin (HE) staining. Histopathologic observations were performed with a biocular light microscope, then for shooting using photomicrograph. The data obtained were analyzed descriptively. Edema, fat degeneration, necrosis, erotion of intestine villi and lisis of intestine villi were found as the result of intestine histopathologic test. This result showed that higher lead concentration causes more severe intestine damage that occur. From this result can be concluded that 6,26 mg/l, 12,53mg/l and 25,06 mg/l causes tissue damage histopathologically.
ANGKA PREVALENSI CEMARAN BAKTERI Escherichia coli PADA TANGAN PEDAGANG DAGING AYAM BROILER DI DUA PASAR TRADISIONAL KOTA BANDA ACEH (The Prevalence Rate of Escherichia coli Contamination on Hand of Broiler Meat Traders at Two Traditional Markets in Banda Aceh) pani noveliani lubis; T Reza Ferasyi; Rastina Rastina; muhammad isa; Nazaruddin Nazaruddin; etriwati etriwati
JURNAL ILMIAH MAHASISWA VETERINER Vol 4, No 3 (2020): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v4i3.15178

Abstract

ABSTRAKSumber pencemaran Escherichia coli pada produk pangan salah satunya yang penting yaitu diakibatkan oleh kontaminasi tangan. Penelitian ini bertujuan untuk mengetahui cemaran dari tangan pedagang dan angka prevalensi cemaran bakteri Escherichia coli pada tangan pedagang daging ayam broiler di dua pasar tradisional Kota Banda Aceh dengan metode Replicate Organism Direct Agar Contact (RODAC).Sampel yang digunakanadalah 20 tangan pedagang daging ayam broiler. Tangan langsung ditempelkan pada media Eosin Methylen Blue Agar (EMBA) dan selanjutnya diinkubasi dengan suhu 37˚C selama 24 jam. Jumlah koloni bakteri yang tumbuh dihitung untuk mengetahui angkap revalensi. Dari 20 sampel tangan pedagang yang diperiksa, adanya cemaran Escherichia coli pada 9 sampel tangan pedagang. Dapat disimpulkan bahwa tangan pedagang daging ayam broiler tercemar Escherichia coli dengan angka prevalensi keseluruhan 45%.Kata Kunci : Escherichia coli, cemaran, tangan pedagang ABSTRACTOne important source of Escherichia coli pollution in food products is caused by hand contamination. The purpose of this study to determine the contamination from the hands of traders and the prevalence rate of Escherichia coli contamination in the hands of broiler meat traders in two traditional markets in Banda Aceh City with the Replicate Organism Direct Agar Contact (RODAC) method. The sample used was 20 hands of broiler meat traders. The hands were placed on Eosin Methylen Blue Agar (EMBA) media and then incubated at temperature 37˚C for 24 hours. The number of bacterial colonies that grow is calculated to determine the prevalence rate. From the 20 samples of traders hands examined, there were Escherichia coli contaminations in 9 samples of traders hands. It can be concluded that the hands of broiler chicken meat traders are contaminated Escherichia coli with an overall prevalence rate of 45%.Keywords: Escherichia coli, contamination, traders hand
PENGARUH PAPARAN TIMBAL (Pb) TERHADAP HISTOPATOLOGIS INSANG IKAN NILA (Oreochromis nilloticus). The Effect of Lead (Pb) Exposure to the Histopathology of Nile Tilapia (Oreochromis nilloticus) Gill Rosmaidar Rosmaidar; Nazaruddin Nazaruddin; T Armansyah TR; Ummu Balqis; Yudha Fahrimal
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.999 KB) | DOI: 10.21157/jim vet..v1i4.5091

Abstract

Penelitian ini bertujuan mengetahui pengaruh paparan timbal (Pb) terhadap histopatologis insang  ikan nila (Oreochromis nilloticus). Penelitian ini menggunakan ikan nila sebanyak 12 ekor dengan kriteria: sehat, bobot badan 15-18 gram, umur ± 2 bulan, jenis kelamin jantan. Penelitian ini merupakan penelitian eksperimental laboratorik dengan desain rancangan acak lengkap yang terdiri atas 4 kelompok perlakuan masing-masing dengan 3 ulangan. Semua kelompok diberikan pakan pelet. Kelompok P0 sebagai kontrol, ikan hanya diberi pakan pelet, P1 diberikan paparan timbal  6,26 mg/l, P2 diberikan paparan timbal 12,53 mg/l, dan P3 diberikan paparan timbal 25,06 mg/l. Perlakuan dilakukan selama 30 hari, dan pengambilan organ insang dilakukan pada hari 31. Sampel insang kemudian diambil dan difiksasi dalam larutan Davidson 10% dilanjutkan dengan pembuatan sediaan histopatologis dan pewarnaan hematoksilin dan eosin (HE). Pengamatan histopatologis dilakukan dengan mikroskop cahaya biokuler, kemudian untuk pengambilan gambar dengan menggunakan fotomikrograf. Data yang diperoleh dianalisis secara deskriptif.  Hasil pemeriksaan histopatologis insang ditemukan edema, kongesti, nekrosis, hiperplasia lamela sekunder, dan fusi lamela. Dari hasil ini menunjukkan bahwa semakin tinggi kosentrasi timbal yang diberikan semakin parah kerusakan organ insang yang terjadi. Berdasarkan hasil penelitian dapat disimpulkan bahwa kosentrasi timbal dapat meningkatkan kerusakan jaringan secara histopatologis.The aims of this research was to determine The effect of lead (Pb) exposure to the histopathology of Nile tilapia (Oreochromis nilloticus) gill. This study used 40 tilapia fish with criteria: healthy; body weight 15-18 gram; age ± 2 month; male sex. This study, is a laboratory experiment (in vivo) using complete randomized design with 4 treatments groups, each group were repeated 3 times. Each groups were fed with pellet. P0 as control, fish fed only pellets, P1 is given lead exposure 6,26 mg/L and pellet feed, P2 is given lead exposure 12,53 mg/L, P3 is given lead exposure 25,06 mg/L. Treatment carried out for 30 days, and fish were  euthanized on  the 31st days. Gill samples were then collected and fixed in Davidson 10% solution followed by histopathology preparation using haematoxylin and eosin (HE) staining. Histopathologic observations were performed using a biocular light microscope, then for using photomicrograph. The data obtained were analyzed descriptively. The results of gill histopathologic examination found edema, congestion, necrosis, primary and secondary lamellae hyperplasia, and lamellae fusion. The result showed that the higher lead concentration the more severe damage on fish gills.  Based on result of this research can be conclided that lead concertration can increased tissue demage histopathologically.
PENGARUH PAPARAN TIMBAL (Pb) TERHADAP HISTOPATOLOGIS HATI IKAN NILA (Oreochromis nilloticus) ( The effect of lead (Pb) exposure to the histopathology of Nile tilapia (Oreochromis nilloticus) liver) Rosmaidar Rosmaidar; Nazaruddin Nazaruddin; winaruddin winaruddin; Ummu Balqis; T. Armansyah TR
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.944 KB) | DOI: 10.21157/jim vet..v1i4.4992

Abstract

ABSTRAK Penelitian ini bertujuan mengetahui pengaruh paparan timbal  terhadap histopatologis hati ikan nila (Oreochromis nilloticus).Penelitian ini menggunakan ikan nila sebanyak 12 ekor dengan kriteria: sehat; bobot badan 10 – 20 gram; umur ± 2 bulan; jenis kelamin jantan.Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) pola searah dengan 4 kelompok perlakuan dan 3 ulangan. Semua kelompok diberikan pakan pelet yang diberikan setiap hari. Kelompok P0 sebagai kontrol, ikan hanya diberi pakan pelet, P1 diberikan paparan timbal 6,26 mg/l, P2 diberikan paparan timbal 12,53 mg/l dan P3 diberikan paparan timbal 25,06 mg/l.Perlakuan dilakukan selama 30 hari dan ikan dietanasi pada hari ke-31. Hasil menunjukan rata-rata (± SD) jumlah sel-sel hepatosit hati pada ikan  yang mengalami degenerasi P0 (0,93 ± 0,61); P1 (18,73 ± 4,95); P2 (22,33 ± 4,57);dan P3 (44,80 ± 5,20); dan nekrosis P0 (0,73 ± 0,50); P1 (8,53 ± 2,50); P2 (8,93 ± 0,83); dan P3 (16,73 ± 2,00). Dari hasil penelitian dapat disimpulkan bahwa paparan timbal dengan dosis 25,06 mg/l menyebabkan kerusakan degenerasi dan nekrosis yang berat secara histopatologis pada sel hepatosit hati ikan nila (Oreochromis nilloticus).ABSTRACT The aims of this research was to find out to determine the level of lead (Pb) concentration that affect the rate of growth tilapia fish (Oreochromis nilloticus). This study used twelve tilapia fish with criteria: healthy; body weight 10-20 gram; age ± 2 month; male sex. The research was designedby a completely randomized design (CRD) with 4 unidirectional patterngroup treatments and three reins. All groups are given pellet feed given daily. GroupP0 as control, fish fed only pellets, P1 is given lead exposure 6,26 mg/l, P2 is given lead exposure 12,53 mg/l and P3 is given lead exposure 25,06 mg/l. Treatment carried out for 30 days andfish were euthanized on day 31. The statistical analysis showed that the average (± SD) number of hepatocyte liver cells in fish with degeneration wereP0 (0.93 ± 0.61), P1 (18.73 ± 4.95), P2 (22.33 ± 4.57), and P3 (44.80 ± 5.20), and necrosis were P0 (0.73 ± 0.50), P1 (8.53 ± 2.50), P2 (8.93 ± 0.83), and P3 (16.73 ± 2.00). From the results of the study can be concluded lead exposure with dose 25,06 mg/l cause damage degeneration and necrosis which is heavily histopatological in hepatocytes cell of Nile tilapia (Oreochromis nilloticus) liver.
Histopathologic Features of Trachea and Lungs in Chickens with Chronic Respiratory Disease Aiza Annisa; Darmawi Darmawi; Etriwati Etriwati; Ummu Balqis; M. Nur Salim; Nazaruddin Nazaruddin; Dwinna Aliza; Siti Aisyah; Awaluddin Awaluddin; Denny Irmawati Hasan; Muslim Akmal; T. Zahrial Helmi; Nuzul Asmilia
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.37429

Abstract

The purpose of this case study is to determine the changes that exist in chickens infected with Chronic Respiratory disease (CRD). A broiler male chicken aged ± 40 days was found dead in a chicken basket at Ulee Kareng chicken slaughterhouse in a state of feather loss and feces attached to cloaca, on January 6th, 2023. The chicken was brought to pathology laboratory of Faculty of Veterinary Medicine, Universitas Syiah Kuala for necropsy and organ collection. Organs associated with CRD cases were trachea and lungs and histopathological preparations were carried out, stained with Haematoxylin Eosin, and observed under a microscope. The results of histopathological examination showed hemorrhage and hyperplasia of the tracheal epithelium. Hemorrhage was caused by edema which causes increased vascular permeability and epithelial cell hyperplasia occured due to the body's response to infection or inflammation. In the lungs there was bleeding due to damage to the blood vessel wall which caused increased vascular permeability, so that fluid and blood plasma come out of the blood vessels. Fibrinogen then underwent polymerization which resulted in fibrinous thrombi. Congestion happened as the result of fluid accumulation in a location that occured in a passive process so the fluid failed to escape. Because of the accumulation of fluid (congestion), the blood supply to the lung tissue is interrupted, resulting in several points of necrosis in the lungs.
Co-Authors Aiza Annisa Awaluddin Awaluddin Darmawi Darmawi Denny Irmawati Hasan Dwinna Aliza Etriwati E M. Nur Salim muhammad isa Muslim Akmal Nuzul Asmilia pani noveliani lubis Rastina Rastina Rosmaidar Rosmaidar Siti Aisyah T Armansyah TR T Reza Ferasyi T. Armansyah TR T. Zahrial Helmi Ummu Balqis winaruddin winaruddin Yudha Fahrimal