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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Ilmu Lingkungan Prosiding Seminar Biologi Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Journal of Natural Sciences and Mathematics Research Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Scripta Biologica Indigenous Biologi Journal of Science and Science Education Bioed : Jurnal Pendidikan Biologi Engagement: Jurnal Pengabdian Kepada Masyarakat International Journal of Economics, Business and Innovation Research Jurnal Pengabdian Kepada Masyarakat
Vincentia Irene Meitiniarti
Universitas Kristen Satya Wacana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences

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Decolorization of Orange II by Mixed Culture of Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 VINCENTIA IRENE MEITINIARTI; KRIS HERAWAN TIMOTIUS; ENDANG SUTARININGSIH SOETARTO; EKO SUGIHARTO
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.238 KB) | DOI: 10.5454/mi.6.3.4

Abstract

Previous work showed that Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 were able to decolorize the orange II qualitatively. In that experiment, E. faecalis could decolorize orange II more rapidly than C. indologenes. The objective of this study was to examine the decolorization of orange II by mixed culture and the growth of both bacterial species on orange II containing medium. The experiment was done in 500 mL sterilized Erlenmeyer flasks containing 285 mL growth media with 80 mg L-1 orange II. Five different treatments were performed in this project, i.e. medium was inoculated with (i) 15 mL of sterile aquadest, (ii) 15 mL of C. indologenes, (iii) 15 mL of E. faecalis, (iv) 7.5 mL of C. indologenes and 7.5 mL of E. faecalis, and (v) 7.5 mL of E. faecalis until decolorization occured , followed by inoculation with 7.5 mL of C. indologenes. Bacterial growth (total cells number), orange II, glucose, and suphanilic acid, as intermediate product of orange II decolorization, concentrations were measured every 2 h.The maximum decolorization of orange II was observed in the medium inoculated with a mixed culture of E. faecalis and C. indologenes. Decolorization of orange II occurred of growth and gave final concentration of sulphanilic acid of 7.06 mg L-1. During culture both species grow in equilibrium in terms of population.
Dekolorisasi pewarna tosca menggunakan koagulan ferro sulfat dan lumpur aktif dari pabrik teksil di salatiga pada kondisi aerob Agustien Sri Noerwahju; Vincentia Irene Meitiniarti; Sri Kasmiyati
Jurnal Ilmu Lingkungan Vol 17, No 3 (2019): November 2019
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.584 KB) | DOI: 10.14710/jil.17.3.500-506

Abstract

Industri tekstil mengeluarkan air limbah dengan kualitas COD, padatan tersuspensi, warna yang relatif buruk dan mengandung logam berat yang bergantung pada zat warna yang digunakan. Saat ini Perda Prop Jawa Tengah no 5 tahun 2012, belum mengatur parameter warnasebagai parameter uji namun limbahberwarna harus tetap diolah karena menyangkut masalah estetika. Salah satu pewarna yang sulit didegradasikan melalui pengolahan biologi maupun kimia/fisika adalah pewarna Turquise Blue atau Tosca. Penelitian ini bertujuan untuk mempelajari pengurangan absorbansi warna, kandungan COD dan TSS pada limbah tekstil sintetik yang mengandung pewarna tosca dengan penambahan ferro-sulfat dan lumpur aktif pada waktu tinggal tertentu dengan proses aerob. Penelitian ini menggunakan limbah cair sintetik yang mengandung pewarna tosca dengan konsentrasi warna yang sama dengan warna air limbah aslinya (absorbansi warna diukur pada l 536 nm). Pengolahan dilakukan dengan mengkombinasikan proses Ferro-sulfat dan lumpur aktif pada kondisi aerob dengan sistem Sequencing bacth reactor (curah berurutan). Pengambilan sampel dilakukan pada jam ke 48, dan jam ke 72. Parameter yang diamati adalah absorbansi warna, COD dan TSS. Efisiensi pengurangan parameter tersebut dibandingkan pada semua perlakuan. Dari hasil penelitian didapat efisiensi penurunan terbaik untuk COD, TSS, dan absorbansi warna, berturut-turut adalah sebesar 79, 60, dan 52% pada pengolahan jam ke 72.
Bioremediation of Pb and Cd contaminated soil using microorganism Andriyani Dea Wulandari; Vincentia Irene Meitiniarti
Journal of Science & Science Education Vol 5 No 1 (2021): JoSSE Vol. 5 No. 1 (May 2021)
Publisher : Faculty of Science and Mathematics, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/josse.v5i1p1-11

Abstract

The increase in industrial waste, especially those containing Pb and Cd, which is discharged into the environment results in more polluted environment conditions. Polluted enviornment is very dangerous for the survival of living things. This technique use living things to reduce environmental pollution, making it safe for living things. Bioremediation can be carried out by in-situ and ex-situ methods with several bioremediation mechanisms, including biosorption, bioaccumulation, bioleaching, and bioprecipitation. The use ex-situ techniques in bioremediation is easier to do, especially if it is carried out to remediate soils under controlled conditions in the laboratory.
ISOLASI BAKTERI ASAM LAKTAT DARI KIMCHI DAN KEMAMPUANNYA MENGHASILKAN ZAT ANTI BAKTERI Benedicta Yolanda Khristnaviera; Vincentia Irene Meitiniarti
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.069 KB) | DOI: 10.20884/1.sb.2017.4.3.447

Abstract

South Korea is famous for its fermented food called Kimchi, a traditional Korean food fermented from pickled vegetables with a mixture of spicy seasoning. Kimchi is now one of functional food products because of there are lactic acid bacteria that are probiotic and can produce bacteriocin compounds. These bacteriocin compounds may inhibit or have anti-bacterial activity. The purpose of this study was to obtain isolates of lactic acid bacteria from store-bought kimchi and homemade kimchi, to examine the antibacterial agent produced by lactic acid bacteria isolated from kimchi against Escherichia coli and Staphylococcus aureus bacteria. We conducted a Disk Diffusion Method to tests the bacteriocin activity, and data were descriptively analyzed. The results showed that eight isolates of lactic acid bacteria from store-bought kimchi and homemade kimchi homemade were able to inhibit the growth of tested bacteria, S. aureus and E. coli. Isolate D1 isolated from store-bought kimchi has largest inhibitory capability against S. aureus and E. coli; it has 16.00 mm and 17.33 mm inhibitory zone, respectively. Isolate B2 isolated from homemade kimchi has the most significant inhibitory ability against S. aureus and E. coli; it has 16.67 mm and 17.67 mm inhibitory zone, respectively. The lowest ability to form clear zone was found on isolate D2 isolated from homemade kimchi. The inhibitory zone of produce by strain D2 against S. aureus and E. coli were 7.67 mm and 8.67 mm, respectively.
ISOLASI DAN KARAKTERISASI JAMUR LIGNINOLITIK SERTA PERBANDINGAN KEMAMPUANNYA DALAM BIODELIGNIFIKASI Putri Elvira Valencia; Vincentia Irene Meitiniarti
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.275 KB) | DOI: 10.20884/1.sb.2017.4.3.449

Abstract

Fungi can survive in various environments with different media including wood. Lignin in timber is hard to be degraded and hydrolyzed efficiently because of its polymer form, composite and complex structure. Ligninolytic fungi produce an extracellular enzyme to withstand with toxic or mutagenic chemicals exposure and known to degrade different types of pollutant compounds. Lignin decomposers were also known to play a significant role in the pulping process of paper mills, used in waste treatment such as textile and hydrocarbon wastes. This study was conducted to obtain fungal isolates that have delignification capability and to compare the ability of fungal isolates in degrading lignin. Isolates were from rotten wood and soil using selective lignin medium with tannic acid as sole C source. This study characterized the isolates by their morphology and identified them using Morphology and Taxonomy of Fungi book by Bessey (1950). The ligninolytic capability comparison was conducted by measuring the transparent zone formed on selective lignin media. This research found 14 isolates of fungi and all of them had the ligninolytic capability. Aspergillus niger isolate has the highest ligninolytic capability by producing 6.45 cm clear zone diameter on the 7th day of incubation. Aureobasidium sp. has the smallest clear zone diameter of 1.9 cm within the same period.
Antagonistic Effect of Two Indigenous Phosphate Solubilizing Bacteria, Burkholderia contaminans PSB3 and Acinetobacter baumannii PSB11 Isolated From Different Crop Soils RULLY ADI NUGROHO; VINCENTIA IRENE MEITINIARTI; CHRISSEPTINA DAMAYANTI
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.522 KB) | DOI: 10.5454/mi.14.2.1

Abstract

Phosphorus is the most important key element in the nutrition of plants. Although P is abundant in soils, it is a major limiting factor for plant growth as it is in an unavailable form for roots uptake. Phosphate solubilizing bacteria (PSB) has ability to convert insoluble form of P to an available form. This study was aimed at screening and characterizing phosphate-solubilizing bacteria from manure and different rhizosphere and to ascertain a potential benefit to use mixed cultures to improve P solubilization. A total of 12 PSB colonies were isolated on Pikovskaya’s agar medium containing tricalcium phosphate. Out of 12 bacterial isolates, 2 isolates showed high phosphate solubilization index (2.17 and 1.83, respectively) were selected for further study. Based on the 16S rRNA gene sequence analysis, PSB3 was closely related to Burkholderia contaminans (99%), and PSB11 was closely related to Acinetobacter baumannii (99%). The mean P dissolved in liquid cultures of PSB3 and PSB11 in a 14-day incubation were 96.7 and 39.3 mg l-1, respectively. Mixed inoculation of B. contaminans PSB3 and A. baumannii PSB11 could not increase the solubilization activity significantly, suggesting there is antagonistic behavior of one isolate towards another. As the interaction of these two isolates may be antagonistic, co-inoculation of these bacteria for P solubilization is not recommended. However, further study is needed to confirm these results.
Kemampuan Microbacterium sp. strain SpR3 dan Isolat RT-9 pada Bahan Pembawa untuk Mereduksi Cr(VI) di Tanah Aprilia Citra Lestari; Vincentia Irene Meitiniarti
Jurnal Ilmu Lingkungan Vol 21, No 4 (2023): October 2023
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.21.4.735-739

Abstract

Kromium heksavalen memiliki tingkat toksisitas yang lebih tinggi daripada Cr(III). Penanganan tanah yang tercamer krom dapat dilakukan melalui bioremediasi dengan memanfaatkan proses metabolism mikroorganisme. Dalam pemanfaatan mikroorganisme untuk bioremediasi lingkungan tercemar Cr(VI), diperlukan bahan pembawa sebagai habitat sementara. Vermikompos dapat digunakan sebagai bahan pembawa karena mengandung nutrisi yang tinggi. Beberapa penelitian bioremediasi dengan kombinasi beberapa isolate terbukti meningkatkan kemampuan dalam mereduksi polutan. Microbacterium sp. Stain SpR3 diketahui mampu mereduksi krom, namun isolat RT-9 merupakan rhizobakteri yang berlum diketahui kemampuannya dalam mereduksi krom. Kemampuan dan viablitias kultur isolat RT-9 dan kultur campuran dengan SpR3 pada vermikompos belum diteliti. Tujuan dari penelitian ini yaitu untuk menganalisis kemampuan Microbacterium sp. Strain SpR3 dan RT-9 dalam kultur tunggal atai campuran dalam vermikompos sebagai bahan pembawa untuk mereduksi Cr(VI) dalam tanah. Dalam penelitian ini, uji viabilitas dianalisis dengan metode Total Plate Count dan reduksi Cr(VI) secara spektrofotometri. Isolat SpR3 dan RT-9 mampu tumbuh dalam kultur tunggal dan campuran. Hasil penelitian ini menyimpulkan bahwa kultur murni RT-9 dan kultur campur Microbacterium sp. SpR3 mampu menggunakan vermikompos sebagai bahan pembawa dan laju reduksi Cr(VI) pada hari ke-7 dan 14 berturut-turut adalah  0,177 ppm.hr-1 and 0,240 ppm.hr-1
Efisiensi Pengolahan Limbah Pewarna Sumifix Blue Menggunakan Lumpur Aktif dengan Penambahan Enterococcus faecalis pada Kondisi Anaerob-Aerob Yulia Mesak; Vincentia Irene Meitiniarti
Jurnal Ilmu Lingkungan Vol 21, No 4 (2023): October 2023
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.21.4.907-913

Abstract

Sumifix blue (SB) merupakan salah satu pewarna golongan azo yang terdapat dalam limbah tekstil. Pembuangan limbah yang mengandung pewarna tanpa pengolahan terlebih dahulu dapat memunculkan masalah bagi lingkungan dan membahayakan makhluk hidup di sekitarnya. Pengolahan dengan proses lumpur aktif dalam kondisi aerob umum digunakan dalam mengatasi limbah pewarna. Proses lumpur aktif dapat ditingkatkan dengan penggunaan mikroba pengurai pewarna. Dalam penelitian ini ingin diketahui efisiensi lumpur aktif dalam pengolahan limbah pewarna dengan penambahan E. faecalis yang telah terbukti mampu mendekolorisasi pewarna jenis azo. Selain mengkombinasikan lumpur aktif dengan E. faecalis, proses juga diuji dalam kondisi yang berbeda yaitu aerob, anaerob dan campuran (anaerob-aerob). Penelitian ini didesain menggunakan limbah cair sintetik yang mengandung pewarna SB dengan konsentrasi warna yang disesuaikan dengan limbah aslinya (absorbansi warna diukur pada l 536 nm). Pengolahan dilakukan dengan proses lumpur aktif dan kombinasi lumpur aktif dengan E faecalis pada kondisi aerob, anaerob dan campuran (anaerob-aerob) dengan sistem batch. Pengambilan sampel dilakukan setiap selang waktu 24 jam selama 96 jam. Parameter yang diamati adalah konsentrasi warna, COD dan TS. Efisiensi hasil pengurangan parameter tersebut  dibandingkan pada semua perlakuan. Dari hasil penelitian ini dapat disimpulkan bahwa efisiensi pengurangan konsentrasi SB terbaik diperoleh pada proses pengolahan menggunakan lumpur aktif pada kondisi anaerob dengan presentase sebesar 86%, efisiensi pengurangan COD dan TS terbaik diperoleh dari proses pengolahan menggunakan lumpur aktif pada kondisi campuran (anaerob-aerob) dengan presentase berturut-turut sebesar 80% dan 28%. Pada penelitian ini, penambahan E. faecalis tidak terbukti meningkatkan efisiensi pengolahan SB.
Using a Participatory Learning and Action Approach to Improve Young Papuans and Moluccans’ Knowledge of Herbal Drinks to Increase Immunity During the COVID-19 Pandemic Rully Adi Nugroho; Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Vincentia Irene Meitiniarti; Agna Sulis Krave
Engagement: Jurnal Pengabdian Kepada Masyarakat Vol 5 No 2 (2021): November 2021
Publisher : Asosiasi Dosen Pengembang Masyarajat (ADPEMAS) Forum Komunikasi Dosen Peneliti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/engagement.v5i2.788

Abstract

The introduction of coronavirus disease (COVID-19) into the human population represents a tremendous medical and economical crisis. The immune system plays a central role in protecting against this novel virus. This activity aimed to empower young generation using participatory learning and action approach to improve their knowledge about medicinal plants that grow in Indonesia and whose anti-inflammatory, antioxidant, immunomodulatory, and antiviral properties. Organoleptic test results showed that Kunyit Asam (consisted of turmeric and ginger) and Tealang (consisted of butterfly pea and lemongrass) were highly favored by panelists. Our results provide valuable information on the medicinal plants used as an immunity booster within the studied community.
MENGEMBANGKAN KAMPUS HIJAU: KONDISI PADUK PENGELOLAAN LIMBAH DI UNIVERSITAS KRISTEN SATYA WACANA Nugroho*, Rully A.; Meitiniarti, V Irene.; Krave, Agna S.; ,, Djohan; Sukmana, Andreas Binar Aji.; Cahyaningrum, D C.; Hastuti, S P.; Kristiani, Elizabeth Betty Elok.; Keliat, Natalia R.; Kasmiyati, S; ,, Sucahyo; Rahardja, P M.; Putri, A C.
INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI Vol 7 No 2 (2024): INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v7i2.584

Abstract

Saat ini, gerakan kampus hijau dan berkelanjutan mulai menjadi tren baru di berbagai universitas dalam dan luar negeri. Penelitian ini bertujuan untuk mendapatkan gambaran kondisi paduk berdasarkan kriteria limbah (WS) dari UI GreenMetric di Universitas Kristen Satya Wacana (UKSW). Penelitian ini menggunakan kuesioner UI GreenMetric dan wawancara terhadap kepala-kepala direktorat dan laboratorium yang bertanggungjawab terhadap pengelolaan limbah di UKSW, termasuk pihak ketiga yang ditugasi untuk mengelola limbah. Hasil studi menunjukkan bahwa UKSW memiliki program kampus hijau untuk mewujudkan kampus yang ramah lingkungan, UKSW telah mengimplementasikan 3R yang terintegrasi dengan pengelolaan lingkungan kampus dalam rangka mewujudkan lingkungan kampus yang lebih hijau dan berkelanjutan. UKSW direkomendasikan agar ke depan dapat mengimplementasikan strategi-strategi ekonomi sirkuler dalam pengelolaan limbah sehingga dapat mencapai tujuan pengelolaan dan fungsi lingkungan kampus berkelanjutan.
Co-Authors ,, Djohan Agna Sulis Krave Agustien Sri Noerwahju Ana W Purnomo Andreas Binar Aji Sukmana Andriyani Dea Wulandari Aprilia Citra Lestari Benedicta Yolanda Khristnaviera Cahyaningrum, Desti Christian CHRISSEPTINA DAMAYANTI Damara Dinda Nirmalasari Zebua Desti Christian Cahyaningrum Dwianto, Stefanus Agung EKO SUGIHARTO Elizabeth Betty Elok Kristiani ENDANG S. SOETARTO ENDANG SUTARININGSIH SOETARTO ENDANG SUTARININGSIH SOETARTO Gabriela Nindrasari Ie, Johanes Juan Yutama Putra Intan Permatasari Jubhar C. Mangimbulude K H Timotius Kara, Patrisia Ilene Keliat, Natalia Rosa KRIS HERAWAN TIMOTIUS Kris Herawan Timotius Liska Simamora Lusiawati Dewi Maria Marina Herawati Melycorianda H. Ndapamuri Napitupulu, Morina M. Natalia Rosa Keliat Novellasari, Firdiana Priyayi, Desi Fajar Putri Elvira Valencia Putri, A C. Rahardja, P M. Rully Adi Nugroho Saiful Akhyar Lubis Seleiman, Mahmoud F. Sri Kasmiyati Sucahyo Sunardi, E. Vandiyani Susanti Pudji Hastuti Theodorus Olwyn Innation Timotius, K. H. Yulia Mesak Yusak Maryunianta Yusta, G. Maria