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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal Indonesian Journal of Dental Medicine Jurnal Pengabdian Masyarakat Dentmas
Udijanto Tedjosasongko
Department Of Pediatric Dentistry, Faculty Of Dental Medicine, Universitas Airlangga
Dentistry

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Penambahan xylitol dalam glukosa, sukrosa terhadap pertumbuhan Streptococcus mutans (in vitro) (The Additional xylitol in glucose and sucrose on growth of Mutans Streptococci (in vitro)) Susilowati Susilowati; Udijanto Tedjosasongko; FX Suhariadji
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 4 (2014): December 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.077 KB) | DOI: 10.20473/j.djmkg.v47.i4.p181-185

Abstract

Background: Xylitol is a sugar alcohol group consisting of five-carbon chain and the sugar substitutes are recommended to prevent caries. Dietary sugars known as a good substrate for the growth of Streptococcus mutans (S. mutans). Two types of sugar, xylitol and dietary sugars have different effects on the growth of S.mutans. Purpose: The objective of this study were to determine the minimal inhibitory concentration (MIC) of xylitol on the growth of S.mutans and to determine the addition of xylitol in glucose and sucrose in the growth of S. mutans in vitro. Methods: The samples were divided into 3 groups: xylitol group, xylitol and sucrose combination group, and xylitol and glucose combination group . In all groups were tested against S.mutans growth in various concentrations. Results: The minimum inhibitory concentration against S.mutans xylitol was equal to 0.625%. The addition of xylitol in sucrose the inhibition of S.mutans growth occurred at concentrations of 0.625 % and 2.5%. The addition of xylitol in glucose inhibited the growth of S.mutans at all concentrations. Conclusion: This study showed that the combination of xylitol with dietary sugars could inhibit the growth of S.mutans.Latar belakang: Xylitol adalah golongan gula alkohol yang terdiri dari lima rantai karbon dan merupakan sugar substitutes yang dianjurkan untuk mencegah terjadinya karies. Dietary sugars diketahui sebagai substrat yang baik untuk pertumbuhan bakteri rongga mulut salah satunya Streptococcus mutans (S. mutans). Dua jenis gula yaitu xylitol dan dietary sugars memiliki pengaruh yang berbeda pada pertumbuhan S. mutans. Tujuan: Tujuan dari penelitian ini adalah meneliti konsentrasi hambat minimal (Minimal Inhibitory Concentration/ MIC) xylitol terhadap pertumbuhan S mutans dan meneliti pengaruh penambahan xylitol dalam glukosa dan dalam sukrosa terhadap pertumbuhan S. mutans secara in vitro. Metode: Sampel dibagi dalam 3 kelompok: kelompok xylitol, kelompok kombinasi xylitol dan sukrosa, dan kelompok kombinasi xylitol dan glukosa. Pada ketiga kelompok tersebut dilakukan pengujian terhadap pertumbuhan S.mutans dalam berbagai konsentrasi. Hasil: Konsentrasi hambat minimum xylitol terhadap S. mutans adalah sebesar 0,625%. Pada penambahan xylitol dalam sukrosa terjadi penghambatan S. mutans pada konsentrasi 0,625% dan 2,5%. Pada penambahan xylitol dalam glukosa terjadi penghambatan S. mutans pada semua konsentrasi. Simpulan: Penelitian ini menunjukkan bahwa kombinasi xylitol dengan glukosa dan dengan sukrosa dapat menghambat pertumbuhan S.mutans.
Indirect pulp capping in primary molar using glass ionomer cements Murtia Metalita; Udijanto Tedjosasongko; Prawati Nuraini
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 4 (2014): December 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.523 KB) | DOI: 10.20473/j.djmkg.v47.i4.p190-193

Abstract

Background: Indirect pulp capping in primary teeth, however, is more rarely conducted than permanent teeth, since it thought to have low impact and most suggestion is for taking caries lesion aggressively on primary teeth. Purpose: The study was aimed to evaluate the subjective complaint, clinical symptom, and radiographic appearance of indirect pulp capping treatment using glass ionomers cements in primary molar. Methods: Sixteen children in range of age 6 to 8 years old, who visited Clinic of Pediatric Dentistry Universitas Airlangga Dental Hospital, Surabaya Indonesia, were the subject of study. They had one occlusal dental caries on one side of maxillary or mandibular primary molar with the diagnose of pulpitis reversible. The experimental group, had indirect pulp capping treatment with glass ionomer cements (GC Fuji VII®), while the control group, had indirect pulp capping treatment with calcium hydroxide (Metapaste). Each group was filled with GC Fuji IX® as permanent restoration. After one week, one month, and three months later, the observations were made on subjective complaint, clinical symptom, and radiographic appearance. Results: The results showed no subjective complaint such as pain or problem on mastication; no negative clinical symptoms such as pain on palpation, gingivitis or periodontitis, and abnormal tooth mobility; no negative radiographic appearance such as pathological apical radioluscency, internal or external resorbtion, and change of ligament periodontal widthafter the treatment. Conclusion: The study suggested that indirect pulp capping treatment using glass ionomer cement materials on primary teeth might be considered to be the treatment choice.Latar belakang: Indirect pulp capping pada gigi sulung lebih jarang dilakukan dibandingkan gigi permanen, karena dianggap memiliki dampak yang rendah dan sebagian besar menyarankan untuk mengambil lesi karies secara agresif pada gigi sulung. Tujuan: Penelitian ini bertujuan untuk mengevaluasi keluhan subjektif, gejala klinis, dan gambaran radiografi perawatan indirect pulp capping menggunakan glass ionomer pada gigi molar sulung. Metode: Enam belas anak berusia 6 sampai 8 tahun, yang mengunjungi Klinik Kedokteran Gigi Rumah Sakit Gigi dan Mulut Universitas Airlangga, Surabaya Indonesia, adalah subjek penelitian ini. Mereka punya satu karies gigi oklusal molar sulung pada satu sisi maksila atau mandibula dengan diagnosa pulpitis reversibel. Pada kelompok eksperimen dilakukan perawatan indirect pulp capping dengan glass ionomer (GC Fuji VII®), sedangkan kelompok kontrol, dilakukan perawatan indirect pulp capping dengan kalsium hidroksida (Metapaste). Setiap kelompok ditumpat dengan glassionomer untuk gigi posterior (GC Fuji IX®) sebagai restorasi permanen. Observasi dilakukan setelah satu minggu, satu bulan, dan tiga bulan kemudian, pengamatan dilakukan pada keluhan subjektif, gejala klinis, dan gambaran radiografi. Hasil: Hasil penelitian menunjukkan tidak ada keluhan subjektif seperti rasa sakit atau masalah pada pengunyahan; tidak ada gejala klinis negatif seperti rasa sakit pada palpasi, gingivitis atau periodontitis, dan mobilitas gigi abnormal; tidak ada gambaran radiografi negatif seperti radioluscency patologis apikal, resorbsi internal atau eksternal, dan pelebaran periodontal ligamen setelah perawatan. Simpulan: Penelitian ini menunjukkan bahwa perawatan indirect capping menggunakan glass ionomer pada gigi sulung dapat dipertimbangkan sebagai pilihan perawatan.
Usia saat inisial akuisisi Streptococcus mutans dan jumlah erupsi gigi sulung pada anak (Initial acquisition age of Mutans Streptococci and number of erupted primary teeth in children) Citra Adinda; Udijanto Tedjosasongko; Teguh Budi Wibowo
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 4 (2014): December 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.399 KB) | DOI: 10.20473/j.djmkg.v47.i4.p202-205

Abstract

Background: Mutan Streptococci (MS) are considered as major bacteria in human dental caries. Previous experiments reported that MS needs permanent surface to make stable colonization in human oral mouth. Transmission of MS occured directly or indirectly by salivary contact. The younger the child acquired MS the higher dental caries risk of the child. Purpose: The purposes of this study was to determine the age and number of primary teeth erupted during MS initial acquisition in children. Methods: The subjects were 30 infants aged 5 months old of Simomulyo region, Surabaya, East Java, Indonesia. Monthly plaque samples were taken using sterile cotton bud and oral examination were done to check number of primary teeth for 6 months period. The MS isolate were isolated using BHI broth and TYC respectively. The MS identification was done by gram staining and colony morphology. Number of erupted primary teeth was determined by counting the erupted teeth in each month. Results: As the result 83% children acquired MS and 17% children remain free from MS. The mean age of initial acquisition was 7,76±0,96 months and the average number of erupted teeth was two teeth. Conclusion: The study revealed that the mean age of MS initial acquisition in children was 7,76±0,96 month and the colonization of MS was found in children with average 2 primary teeth erupted.Latar belakang: Streptococcus Mutans (SM) merupakan bakteri utama penyebab karies gigi. Percobaan sebelumnya melaporkan bahwa SM membutuhkan permukaan permanen untuk membuat kolonisasi stabil di rongga mulut. Penularan SM terjadi secara langsung atau tidak langsung melalui kontak saliva. Semakin muda anak mengakuisisi SM semakin tinggi resiko terjadinya karies pada anak tersebut. Tujuan: Penelitian ini bertujuan meneliti umur dan jumlah rata-rata gigi erupsi pada masa inisial akuisisi SM pada anak Metode: Subyek penelitian ini adalah 30 bayi berusia 5 bulan di wilayah Simomulyo, Surabaya, Jawa Timur, Indonesia. Sampel plak diambil setiap bulan dengan cotton bud steril. Dilakukan pula pemeriksaan rongga mulut untuk memeriksa jumlah gigi sulung yang erupsi selama 6 periode 6 bulan. SM diisolasi menggunakan BHI broth dan TYC. Identifikasi SM dilakukan dengan pewarnaan gram dan pemeriksaan morfologi koloni. Jumlah gigi erupsi ditentukan dari penghitungan jumlah gigi sulung yang erupsi setiap bulannya. Hasil: Delapan puluh tiga persen anak-anak positif didapatkan SM dan 17% anak-anak tetap bebas dari SM. Studi ini menunjukkan bahwa SM rata-rata usia awal akuisisi anak adalah 7,76 ± 0,96 bulan dan jumlah rata-rata gigi erupsi adalah 2 gigi. Simpulan: Penelitian ini menunjukkan bahwa usia rata-rata inisial akuisisi SM pada anak-anak adalah 7,76±0,96 bulan dan kolonisasi SM ditemukan pada anak-anak dengan rata-rata 2 gigi sulung telah erupsi.
Daya hambat xylitol dan nistation terhadap pertumbuhan Candida albicans (in vitro) (Inhibition effect of xylitol and nistatin combination on Candida albicans growth (in vitro)) Sarah Kartimah Djajusman; Udijanto Tedjosasongko; Irmawati Irmawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 3 (2014): September 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.74 KB) | DOI: 10.20473/j.djmkg.v47.i3.p164-167

Abstract

Background: The growth of Candida albicans can be controlled by using antifungal such as nystatin. These days we found that using antifungal is not enough to control Candida albicans, we also have to control the intake of sugar by using xylitol. Purpose: Purpose of the study was to determine the optimal inhibitory concentration of xylitol-nystatin in the Candida albicans growth. Methods: This was an in-vitro study using an antimicrobial test of serial dilution with xylitol-nystatin and sucrose–nystatin consentration of 1%, 3%, 5%, 7%, 9%, and 10%.Growth inhibition of C. albicans was determined by the inhibition zone of xylitol + nystatin on C. albicans culture media (in vitro) Results: The result of study was the inhibitory consentration of xylitol-nystatin to inhibit Candida albicans growth was 3%-10%. Conclusion: The study showed that combination of xylitol and nystation could inhibit the growth of Candida albicans.Latar belakang: Pertumbuhan Candida albicans dapat dikontrol dengan menggunakan antijamur seperti nistatin. Penggunakan antijamur saja tidak cukup untuk mengontrol Candida albicans, namun perlu pula mengontrol asupan gula dengan menggunakan xylitol. Tujuan: Tujuan dari penelitian ini adalah untuk menentukan konsentrasi hambat optimal xylitol-nistatin dalam pertumbuhan Candida albicans. Metode: Penelitian ini merupakan penelitian in vitro menggunakan uji antimikroba pengenceran serial dengan xylitol-nistatin dan nystatin-sukrosa konsentrasi 1%, 3 %, 5 %, 7%, 9%, dan 10%. Daya hambat pertumbuhan C. albicans diukur dari zona hambat xylitol + nistatin pada media kultur C. albicans (in vitro) Hasil: Konsentrasi penghambatan xylitol-nistatin untuk menghambat pertumbuhan Candida albicans adalah 3-10%. Simpulan: Hasil penelitian menunjukkan bahwa kombinasi xylitol dan nystation bisa menghambat pertumbuhan Candida albicans.
Penurunan jumlah Streptococcus mutans pada saliva anak dengan ortodonti cekat setelah konsumsi yoghurt (Reduction of salivary Mutans Streptococci in children with fixed orthodontic appliance after yoghurt consumption) Dewi Anggreani Bibi; Udijanto Tedjosasongko; Irmawati Irmawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 2 (2014): June 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (501.169 KB) | DOI: 10.20473/j.djmkg.v47.i2.p82-86

Abstract

Background: Orthodontic treatment using fixed appliances in children increases with the case of malocclusion in Indonesia. The patients with fixed orthodontics have higher risks of caries. Purpose: The study was aimed to examine the influences short term daily consumption fruit-flavored yoghurt on salivary mutans Streptococci in pediatric patients during orthodontic treatment with fixed appliances. Methods: This was an experimental laboratory study with a double-blind randomized crossover design. The subjects were 26 children in range of age 11 to 15 years old who were under orthodontic treatment using fixed appliances. Subjects were divided into 2 (two) groups which consist of 13 children each. Group A were asked to consumed a 150 ml of yoghurt Bifidobacterium bifidum and Lactobacillus acidophilus once a day for 2 weeks, while group B were asked to consumed milk once a day for the same period of time. After “washout” period for 2 weeks, the subjects of group A and B crossed over the drink, group A was asked to consumed milk and group B was asked to consumed yoghurt for another 2 weeks. Before and after consuming yoghurt or milk, the subject’s saliva samples were taken and the colonies of mutans Streptococci were counted on TYC media. Results: Statistical analysis showed that on subjects who consumed yoghurt the colony number of mutans Streptococci reduced significantly (p < 0.05). Conclusion: Short-term daily consumption of the probiotic yoghurt containing Bifidobacterium bifidum bacteria and Lactobacillus acidophilus could reduce the number of salivary mutans streptococci in pediatric patient during orthodontic treatment with fixed appliances.Latar belakang: Kebutuhan perawatan ortodonti menggunakan alat cekat pada anak-anak meningkat seiring bertambahnya jumlah kasus maloklusi di Indonesia. Namun faktanya penggunaan piranti ortodonti cekat berisiko terjadinya karies disekitar bracket. Tujuan: Penelitian ini bertujuan meneliti pengaruh konsumsi yoghurt buah dalam jangka pendek terhadap jumlah Streptococcus mutans pada saliva anak pemakai ortodonti cekat. Metode: Jenis penelitian ini eksperimental laboratoris dengan desain penelitian double blind randomized crossover design. subjek penelitian adalah 26 anak berusia 11-15 tahun yang sedang menjalani perawatan ortodonti dengan piranti cekat. subjek dibagi dalam 2 kelompok dengan masing-masing 13 anak. Kelompok A diminta untuk mengkonsumsi 150 ml yoghurt Bifidobacterium bifidum dan Lactobachillus acidophillus sekali sehari selama 2 minggu, sedang kelompok B diminta untuk mengkonsumsi susu sekali sehari selama 2 minggu. Setelah periode “washout” selama 2 minggu, kedua kelompok bertukar minuman, kelompok A mengkonsumsi susu sedang kelompok B mengkonsumsi yoghurt selama 2 minggu. Setiap sebelum dan sesudah mengkonsumsi yoghurt dan susu, sampel saliva subjek diambil dan dilakukan penghitungan jumlah koloni Streptococcus mutans pada media TYC. Hasil: Analisa statistik menunjukkan bahwa setelah mengkonsumsi yoghurt jumlah koloni Streptococcus mutans dalam saliva subjek berkurang secara signifikan (p < 0,05). Simpulan: Konsumsi probiotik yoghurt yang mengandung Bifidobacterium bifidum dan Lactobachillus acidophillus dapat mengurangi jumlah koloni Streptococcus mutans dalam saliva anak selama perawatan ortodontik cekat.
Karakterisasi stem cell pulpa gigi sulung dengan modifikasi enzim tripsin (The characterization of stem cells from human exfoliated deciduous teeth using trypsin enzym) Tri Wijayanti Puspitasari; Tania Saskianti; Udijanto Tedjosasongko
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 2 (2014): June 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.465 KB) | DOI: 10.20473/j.djmkg.v47.i2.p115-119

Abstract

Background: Now a days, treatment in dentistry, using tissue regeneration that based on the stem cells from human exfoliated deciduous teeth (SHED), grows rapidly. For several reason, the isolated and cultured SHED is difficult to be applied in Indonesia, therefore the modification is needed. This difficulties were caused by the pulp anatomy, the heterogeneous populations in the pulp chamber and the limitations of tools and materials at the laboratory. Purpose: This research was aimed to examine that the modifications of isolation and culture technique of SHEDs for characterization by using the marker of CD105. Methods: The research was experimental laboratory with the cross sectional design. The samples were the human exfoliated deciduous teeth from the children patients of Pediatric Dentistry Department of Universitas Airlangga Dental Hospital which matched the criteria. Dental pulps were isolated and cultured by using the modifications of Trypsin enzymes. Results: The healthy SHEDs could be produced from the modifications of isolation and culture and positively shown the expression of marker CD105 which were indicated by the fluorencent microscope. Conclusion: SHED which isolated and cultured by using the modified techniques, positively characterized by using marker CD105.Latar Belakang: Pengobatan kedokteran gigi berkembang dengan pesat terutama di bidang regenerasi jaringan berbasis Stem Cells from Human Exfoliated Deciduous Teeth (SHED). Di Indonesia, isolasi dan kultur SHED sulit sehingga perlu dilakukan modifikasi. Kendala ini muncul karena jaringan pulpa yang kecil, heterogen dan keterbatasan alat dan bahan di laboratorium. Tujuan: Penelitian ini bertujuan untuk meneliti modifikasi pada cara isolasi dan kultur SHED untuk karakterisasi menggunakan maker CD105. Metode: Jenis penelitian ini adalah eksperimental laboratoris dengan rancangan cross sectional. Sampel penelitian adalah gigi sulung dari pasien anak di Klinik Kedokteran Gigi Anak, Rumah Sakit Gigi dan Mulut Universitas Airlangga yang telah memenuhi kriteria. Pulpa gigi diisolasi dan dikultur dengan modifikasi enzim Trypsin. Hasil: SHED yang sehat hasil dari modifikasi teknik isolasi dan kultur positif menunjukkan ekspresi marker CD105 dengan berfluoresensi berwarna hijau dilihat melalui mikroskop fluoresen. Simpulan: SHED yang dikultur dan diisolasi dengan teknik modifikasi positif dikarakterisasi dengan marker CD105.
Treatment of non-vital primary molar using lesion sterilization and tissue repair (LSTR 3Mix-MP) Tania Saskianti; Udijanto Tedjosasongko; Irmawati Irmawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 2 (2013): June 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.402 KB) | DOI: 10.20473/j.djmkg.v46.i2.p80-84

Abstract

Background: Root canal preparation and anatomic variations of deciduous teeth often cause the child patient uncooperative and sometimes the treatment failure. the non-threatening treatment and non-invasive approaches is needed to obtain a good cooperation from child patient. Purpose: The study was aimed to clinically evaluate the use of 3Mix-MP- a combination of antibacterial drugs, i.e. metronidazole, minocycline and ciprofloxacin (3Mix), and macrogol and propylene glycol (MP) - as pulp medicament on a necroses primary molar. Methods: Subject were the children patients of Pediatric Dental Clinic Universitas Airlangga Dental Hospital. Eight primary molars with pulp necroses due to dental caries were selected as samples. The treatment was done based on the concept of lesion sterilization and tissue repair (LSTR) therapy. A slice of 3 Mix-MP pastes was placed in the cavity and then sealed with glassionomer cement. Subjects were asked for recall visit in 1, 3 and 6 months post treatment, for clinical and radiographic evaluation. The antibacterial effect of 3 Mix-MP was compared with tempophore on mixed bacteria of pulp cavity which was isolated prior to therapy. The antibacterial effect was determined by measuring the inhibition zone after 24 hours anaerobe incubation. Results: Seven out of 8 subjects on recall visit showed no acute or chronic clinical symptoms, such as fistulae, abscess, purulent exudates, swelling or feel any pain during mastication. Microbiological test result showed LSTR 3Mix-MP had antibacterial effect higher than tempophore (p<0.001). Conclusion: The study revealed that 3Mix-MP treatment showed clinical and radiographic positive response on necrose primary molar.Latar belakang: Preparasi saluran akar dan variasi anatomi gigi sulung seringkali menyebabkan pasien anak tidak kooperatif dan kadang menyebabkan kegagalan perawatan. Perawatan yang tidak menakutkan dan non-invasif diperlukan untuk mendapatkan kerjasama yang baik dari pasien anak. Tujuan: Penelitian ini bertujuan untuk evaluasi klinis penggunaan 3Mix - MP- kombinasi obat antibakteri, yaitu metronidazole, minocycline dan ciprofloxacin (3Mix), dan makrogol dan propilen glikol (MP) – sebagai pengobatan pulpa pada gigi molar sulung yang nekrose. Metode: Subjek adalah pasien anak dari Klinik Kedokteran Gigi Anak Rumah Sakit Gigi & Mulut Universitas Airlangga. Delapan gigi molar sulung dengan nekrosis pulpa akibat karies gigi dipilih sebagai sampel. Pengobatan dilakukan berdasarkan konsep lesion sterilization and tissue repair (LSTR). Selapis pasta 3 Mix - MP ditempatkan di kavitas gigi dan kemudian ditumpat dengan semen glass-ionomer. Subjek diminta untuk kontrol untuk evaluasi klinis dan radiografi 1, 3 dan 6 bulan pasca perawatan. Efek antibakteri 3 Mix - MP terhadap bakteri campuran rongga pulpa yang diisolasi sebelum terapi dibandingkan dengan tempophore . Efek antibakteri ditentukan dengan mengukur zona hambat setelah 24 jam inkubasi anaerob. Hasil: Tujuh dari 8 subjek pada saat kontrol tidak menunjukkan gejala klinis akut atau kronis, seperti fistula, abses, eksudat purulen, bengkak atau merasa sakit selama pengunyahan. Hasil uji mikrobiologi menunjukkan LSTR 3Mix - MP memiliki efek antibakteri yang lebih tinggi daripada tempophore (p<0,001). Simpulan: Hasil penelitian menunjukkan bahwa penggunaan 3Mix - MP memberikan respon positif pada perawatan molar sulung yang nekrotik secara klinis dan radiografis.
The development of early childhood caries impact on quality of life-Indonesia instrument as assessment instrument of dental caries impact on quality of life of children aged 3-5 years based on Indonesian community characteristics Taufan Bramantoro; Yayi Suryo Prabandari; Djauhar Ismail; Udijanto Tedjosasongko
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 4 (2015): December 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.447 KB) | DOI: 10.20473/j.djmkg.v48.i4.p197-203

Abstract

Background: Improvement of quality of life is one of objectives in establishment of healthcare system according to World Health Organization policy. Studies of dental health-related factors that influence quality of life of children aged 3-5 years have not been developed yet, particularly in Indonesia. Previous study which adopted international instruments has some limitations of inappropriate characteristics of question items with local community characteristics. Quality of life assessment could describe sociodemographic status and community cultural background, as well as varies of special characteristics in a community group, related to oral and dental health features with sensitivity to the difference of age group. Purpose: This study was aimed to develop early childhood caries impact on quality of life-Indonesia (ECCIQOL-INA) as assessment instrument of dental caries impact on quality of life of children aged 3-5 years based on Indonesian community characteristics. Method: Initially, this study was conducted qualitatively using Focus Group Discussion method, validity and reliability test, subsequently the study was continued using quantitative method as a cross-sectional analytical study to analyze the utilization of instrument in 309 children aged 3-5 years enrolled in kindergarten and early childhood education programs (ECEP) with their mothers at working area of Community Health Centre Wates in Mojokerto. Result: There were four question items as assessment instrument of dental health problems that impact on quality of life on children aged 3-5 years, i.e, “Has your child ever been irritable/restless?” (“Apakah anak Ibu pernah merasa rewel/gelisah?”), “Has your child ever refused to eat and drink or felt discomfort while eating and drinking?” (“Apakah anak Ibu pernah tidak mau atau tidak enak makan dan minum?”), “Has your child ever been absent from school?” (“Apakah anak Anda pernah tidak masuk sekolah?”) “Has your child ever been difficult or unable to sleep?” (“Apakah anak Ibu pernah tidak bisa tidur atau sulit tidur?”). Conclusion: ECCIQOL-INA instrument had been successfully developed and could be utilized as assessment instrument of dental caries impact on quality of life on children aged 3-5 years based on Indonesian community characteristics.
Streptococcus mutans detection on mother-child pairs using matrix-assisted laser desorption ionization – time of flight mass spectrometry and polymerase chain reaction Udijanto Tedjosasongko; Dwi Mulia Ramadhaniati; Seno Pradopo
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 1 (2021): March 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i1.p52-56

Abstract

Background: Streptococcus mutans (S. mutans) bacteria mainly cause dental caries in children. These bacteria are not considered oral indigenous bacteria since they are transmitted from people around children during their deciduous teeth eruption. The detection of these bacteria can be used for dental caries prevention in children. Purpose: To determine the strain and serotype of S. mutans by using matrix assisted laser desorption ionization – time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) on dental plaque samples taken from mother-child pairs. Methods: Sixteen dental plaque samples of mother-child pairs were cultured on brain heart infusion broth (BHIB) and mitis salivarius bacitracin (MSB) media until S. mutans colony isolates were obtained. Next, the isolates of S. mutans colony were introduced into the target plates of MALDI-TOF MS, and then ionized to become peptide mass fingerprint (PMF). Afterwards, the colony isolates were detected by database software. The detected S. mutans DNA then was extracted by using conventional 727 bp PCR (serotype C). Results: Six strains of S. mutans were detected by MALDI-TOF MS method. Five samples were classified into UA159, two samples were 3SN1, two samples were NFSM1, two samples were 11A1, two samples were U138, two samples were 4SM1, and one sample was classified into another bacterium. Five out of 16 samples were detected by PCR as serotype C (UA159). Conclusion: Six strains of S. mutans were detected, namely UA159, 3SN1, NFSM1, 11A1, U138, and 4SM1, one of them (UA159) was detected as serotype C.
The Role Of Nitrite Producing Bacteria And Its Mechanism To Prevent The Dental Caries Maghfira Izzani Maulania; Dimas Prasetianto Wicaksono; Jason Patrick Siregar; Feby Ayu Lestari; Lukas Aldhi Hannawan Siswoyo; Udijanto Tedjosasongko
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.79-83

Abstract

Background : Dental caries is the most common disease and often occurs in the oral cavity that can be found in adults nor children and until now many studies about dental caries have been established to reduce the caries level. In our daily life, nitrate (NO3-) can be found in the green vegetables which can be reduced to NO2- by some oral bacteria. NO2- can inhibit the growth and acid production of pathogenic bacteria such as Streptococcus mutans to prevent the dental caries. There have been many studies that explain the effect of NO2- as an antibacterial agent, but there are limited studies that explain about the role of NO2- on maintaining the oral health and its relation to the NO3-reduction mechanism involved the environment in the oral cavity. Purpose : To analyze the role of NO2- producing bacteria and its mechanism to prevent the dental caries. Results : The amount of NO2- in the oral cavity produced by reduction of NO3- depends on diet and the number of NO3- reducing bacteria, especially obligate and facultative anaerobes. NO2- can inhibit the growth and metabolism of cariogenic bacteria. Conclusion : NO3- reducing bacteria in the oral cavity, a diet consisting of NO3- plays an important role in the prevention of dental caries through the NO3-- NO2- mechanism to reduce the number and metabolism of cariogenic bacteria.
Co-Authors Anggraini, Ratri Aprillia, Zita Ardiana, Hana Ai Ardianti Maartrina Dewi Az Zahra, Alivy Aulia Batsyeba, Rachel Citra Adinda Dewi Anggreani Bibi Dharmawan, Devi Dimas Prasetianto Wicaksono Djauhar Ismail Dwi Mulia Ramadhaniati Feby Ayu Lestari FX Suhariadji Hana Ai Ardiana Hidemi Kurihara I Komang Evan Wijaksana Irma Josefina Savitri Irmawati Irmawati Jason Patrick Siregar Kazuhisa Ouhara Khairani, Firli Cahaya Laosuwan, Kittipong Leo, Leviena Merlynike Leviena Merlynike Leo Limbri, Stephanie Clara Lukas Aldhi Hannawan Siswoyo Maghfira Izzani Maulania Masyithah Masyithah, Masyithah Mega Moeharyono Puteri Muhammad Nur Falah Murtia Metalita Nelwan, Sindy Cornelia Noriyoshi Mizuno Nunthawan Nowwarote Octaviana, Surya Anastasya Okkinardo Arief Olivia Bambang Siswanto Prawati Nuraini Putra Indra, Rayhan Akbar Rahmawati, Siti Irma Ramadhaniati, Dwi Mulia Reviany, Meylan Rizki Amalia Sarah Kartimah Djajusman Seno Pradopo Setiawati, Herautami Caezar Yulia Siti Rahmawati Soegeng Wahluyo, Soegeng Sudarsono, Wahyudi Susilowati Susilowati Tania Saskianti Taufan Bramantoro Teguh Budi Wibowo Tri Wijayanti Puspitasari Wahyudi, Irene Anastasia Welly Anggarani, Welly Yayi Suryo Prabandari