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Cultivation and expansion of mesenchymal stem cells from human gingival tissue and periodontal ligament in different culture media Banun Kusumawardani; Dwi Merry Christmarini Robin; Endah Puspitasari; Irma Josefina Savitri; Dea Ajeng Pravita Suendi
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 1 (2021): March 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i1.p39-45

Background: Gingival tissue and periodontal ligament act as sources of mesenchymal stem cells (MSCs) that play a vital role in periodontal regeneration, but they both have limitations for cell availability. MSCs cultivated and expanded in various media formulations could be used as a basis for the development of cell therapy protocols. Purpose: This study aimed to determine the optimum culture media formulation for cultivation and expansion of human gingival-derived mesenchymal stem cells (hGMSCs) and human periodontal ligament stem cells (hPDLSCs). Methods: The hGMSCs and hPDLSCs were obtained from gingival tissue and periodontal ligament specimens from an adult patient. The two different culture media formulations used were: 1) α-minimum essential media (α-MEM) supplemented with 10% FBS, 100 U/mL penicillin, 100mg/mL streptomycin and 2.5 µg/mL amphotericin B; and 2) Dulbecco’s minimum essential media-Low Glucose (DMEM-LG) supplemented with 10% FBS, 2 mMol/L L-glutamine, 100 U/mL penicillin, 100mg/mL streptomycin and 2.5 µg/mL amphotericin B. The minced-gingival tissue and periodontal ligament samples were seeded in 3 cm tissue culture dishes with one of two experimental culture media, and incubated at 37oC in a humidified atmosphere of 5% CO2. Results: Cell morphology was observed on days two and five of the third passage. The gingival tissue and periodontal ligament primary cells exhibited fibroblast-like morphology, long processes and were spindle-shaped. The hPDLSCs grown in α-MEM exhibited a significant increase in cell viability and proliferation rate compared to the hPDLSCs grown in DMEM-LG. However, hGMSCs displayed similar cell viability and proliferation rate on both types of experimental media. Both the hGMSCs and hPDLSCs expressed MSC markers, including CD105, CD146, and CD90, but did not express CD45. Conclusion: Culture media formulations of α-MEM and DMEM-LG can be used for the cultivation and expansion of both hGMSCs and hPDLSCs.

Aesthetic consideration in patient management of severe periodontitis aggravated by oral dexamethasone Irma Josefina Savitri; I Komang Evan Wijaksana; Okkinardo Arief; Muhammad Nur Falah; Udijanto Tedjosasongko; Olivia Bambang Siswanto; Kazuhisa Ouhara; Hidemi Kurihara; Noriyoshi Mizuno
Dental Journal Vol. 56 No. 2 (2023): June 2023
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i2.p73-79

Background: The treatment of severe periodontitis must consider immune responses and local conditions, including the aesthetical aspects. The tooth loss in the anterior area can lead to a psychological issue in some patients, and the daily use of dexamethasone contributes greatly to the severity of aesthetical aspects. Periodontal treatments must be cautious of these aesthetical effects. Purpose: To report the aesthetical consideration in patient management of severe periodontitis aggravated by oral dexamethasone. Case: The 44-year-old female patient reported having tooth mobility in the upper right and left central incisor and lower right and left posterior. Due to the condition, the central anterior needed to be extracted. The patient had seafood allergies and consumed oral dexamethasone periodically to prevent allergic reactions for two years. Case management: The initial periodontal therapy was designed prior to the tooth extraction, socket preservation, and immediate denture on teeth 11 and 21. A metal frame combined with an acrylic denture was designed to support the tooth splint and replace the teeth on the mandible. The patient was treated with 20 mg of sub-antimicrobial-dose doxycycline twice a day for three months, and vitamin E was prescribed once a day. Since dexamethasone may contribute to immune response and osteoclastogenesis, dexamethasone was replaced by cetirizine. Conclusion: The treatment of severe chronic periodontitis must consider immune responses, local conditions, and aesthetical aspects. In this case, the use of dexamethasone might worsen the periodontal breakdown. However, the periodontal treatment, use of host modulation therapy, and replacement of dexamethasone with cetirizine are expected to improve these conditions.

Deteksi Dini dan Pencegahan Kelainan Periodontal Pada Ibu Hamil dan Kader Kesehatan Sebagai Upaya Pencegahan Stunting I Komang Evan Wijaksana; Eka Fitria Agustina; Irma Josefina Savitri; Novita Kusuma Wardhani; Michelle Suhartono; Agung Krismariono
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 2 (2023)
Publisher : Universitas Majalengka

Kota Pasuruan merupakan salah satu kota yang masih ditemukan kasus stunting. Adanya Pandemi COVID-19 turut memberikan dampat negatif pada status stunting di Kota Pasuruan yang pada tahun 2021 tercatat sebanyak 23 persen dari tahun sebelumnya sekitar 19 persen. Salah satu sasaran primer pencegahan stunting adalah ibu hamil. Pada ibu hamil, kesehatan rongga mulut berperan besar terhadap kesehatan secara umum, termasuk untuk pencegahan stunting pada calon buah hatinya. Pada pengabdian masyarakat ini, dilakukan pengabdian kepada masyarakat dalam upaya mencegah stunting melalui pencegahan kelahiran Bayi dengan Berat Badan Lahir Rendah (BBLR) dengan cara meningkatkan pencegahan kelainan didalam mulut pada ibu hamil melalui pelatihan deteksi dini dan pencegahan kelainan periodontal pada ibu hamil dan kader kesehatan sebagai upaya pencegahan stunting di Puskesmas Kandangsapi Kota Pasuruan. Tahapan pengabdian dilakukan meliputi tahap persiapan kegiatan, mengorganisasi kegiatan, tahap edukasi dan pelatihan target sasaran, tahap pembentukan kepemimpinan, koodinasi intersectoral dan tahap akhir evaluasi kegiatan. Peningkatan pengetahuan target sasaran menjadi indikator kuantitatif keberhasilan program yang dievaluasi melalui tes tulis sebelum dan setelah pengabdian dilaksanakan. Kegiatan pengabdian masyarakat dapat meningkatkan kemampuan kognitif maupun psikomotor ibu hamil dan kader kesehatan untuk melakukan deteksi dini dan pencegahan kelainan periodontal sebagai upaya pencegahan stunting.

PERIODONTAL TISSUE HEALTH TRAINING FOR PREGNANT WOMEN AND CADRES AT PASURUAN PRIMARY HEALTH CENTERS TO AVERT STUNTING I Komang Evan Wijaksana; Irma Josefina Savitri; Eka Fitria Augustina; Agung Krismariono
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 7 No. 3 (2023): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v7i3.2023.386-394

In Indonesia, stunting continues to be a problem for public health, even in Pasuruan City. In Pasuruan City, 1,516 kids still run the risk of developing stunting. The Pasuruan City Government works to keep the rate of stunting under 14%. To reduce the likelihood that children with stunting will be born, pregnant mothers are the main focus of prevention efforts. Inflammatory diseases in the mouth can set off pregnancy complications that have the potential to result in preterm birth, low birth weight babies (LBW), and eventually become the cause of stunting in children. This community service is carried out in the form of training and counseling on periodontal tissue and oral health for pregnant women and health cadres at the Pasuruan City Health Center to increase knowledge of stunting prevention in Pasuruan City. Community service was carried out at four health centers in Pasuruan City, namely the Kandangsapi, Bugul Kidul, Kebonsari, and Trajeng Health Centers. The target partners in community service are health cadres and pregnant women in the work area of â€‹â€‹each health center. The form of service carried out is counseling and training. Education initiatives were conducted on the subject of periodontal tissue health, its relationship to pregnancy, the prevalence of LBW, and the possibility of stunting effects on the periodontal tissue health of pregnant women. The cognitive and psychomotor health of cadres and pregnant women can be improved by community service in the form of counseling and training.

Kesiapan Dokter Gigi Anggota PDGI Kota Pasuruan dalam pemberian Host Modulation Therapy sebagai Terapi Penunjang Penyakit Periodontal Irma Josefina Savitri; Noer Ulfah; I Komang Evan Wijaksana; Eka Fitria Augustina; Gilang Rasuna Sabdho Wening; Erza Nudya Elwardah; Imanuel Jeremias Laturiuw
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 4 (2023)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v4i4.6336

Periodontitis, keradangan kronis jaringan penyangga gigi atau periodontal, adalah hasil interaksi dari respon imun tubuh manusia dan bakteri penyebab. Prevalensi periodontitis masih tinggi, mengganggu sistemik dan kualitas hidup, sehingga menjadikan periodontitis sebagai salah satu permasalahan gigi dan mulut yang utama. Selain terapi lokal, Host Modulating Therapy (HMT) diperlukan untuk mengurangi kerusakan jaringan dengan memodulasi respon tubuh manusia. Departemen Periodonsia FKG Unair melaksanakan pengabdian masyarakat berupa seminar HMT dengan sasaran dokter gigi di Kota Pasuruan. Tujuan penulisan artikel adalah melaporkan kesiapan dokter gigi anggota PDGI Kota Pasuruan dalam pemberian HMT sebagai terapi penunjang penyakit periodontal. Metode yang digunakan diawali dengan pemberian materi dan dilanjutkan dengan Focus Group Discussion (FGD). Pengukuran tingkat keberhasilan capaian menggunakan uji korelasi “Drug Prescribing Belief Model” dengan metode “post-test” only. Nilai evaluasi akhir didapatkan rerata peserta adalah 82 yang mengindikasikan bahwa penyampaian materi HMT telah optimal dilakukan oleh penyaji. Walaupun tingkat pengetahuan tinggi, uji korelasi Drug Prescribing Belief Model menunjukkan bahwa nilai “self efficacy” peserta rendah. Kesimpulan yang diberikan adalah dokter gigi di Kota Pasuruan memiliki pengetahuan yang baik dalam pemberian HMT namun kurang memiliki keterampilan dalam standar operasional pemberian HMT. Dari kesimpulan ini disarankan untuk dilakukan pelatihan berkelanjutan dalam hal teknis pemberian HMT

Induced pluripotent stem cells in periodontal reconstructive therapy: A narrative review of pre-clinical studies Reza Dony Hendrawan; Chiquita Prahasanti; Irma Josefina Savitri; Padmini Hari
Dental Journal Vol. 56 No. 4 (2023): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i4.p280-286

Background: Regenerative periodontal surgical therapy faces significant challenges due to the limited ability of the body to regenerate damaged periodontal tissue. One of the primary goals in regenerative periodontal therapy is regaining periodontal tissue attachment after destruction by periodontal disease. Currently, stem cells, harnessing three pivotal components—cells, biomaterials, and growth factors—are widely used in periodontal regeneration. Stem cells can be obtained from various sources, either by isolating cells from bone marrow, teeth, and muscles or through the somatic cell programming method (reprogramming) known as induced pluripotent stem cells (iPSCs). Purpose: This review aims to describe the potential use of iPSCs in the treatment of periodontal defects. Review: Search strategies were developed using the PubMed, LILACS, Scielo, and Wiley online databases during the period of 2012–2022. Ten articles met the inclusion criteria. iPSCs were obtained by inducing somatic cells from both dental and non-dental sources with factors Oct3/4, Sox2, Klf4, and c-Myc. Periodontal tissue regeneration procedures can be augmented with iPSCs. Unlike tooth-based stem cells, iPSCs offer several advantages, such as unlimited cell sources and the capability to differentiate into any cell type, including periodontal tissue. The potential of iPSCs extends to correcting periodontal bone defects and forming new periodontal tissues, such as alveolar bone, cementum, and periodontal ligament. However, iPSCs do have limitations, including the need for clinical trials, cell programming production facilities, and optimization of differentiated-cell functionality. Conclusion: The combined use of iPSCs in cell-based tissue engineering holds vast potential for future periodontal treatment strategies.

Deteksi Dini dan Pencegahan Kelainan Periodontal Pada Ibu Hamil dan Kader Kesehatan Sebagai Upaya Pencegahan Stunting I Komang Evan Wijaksana; Eka Fitria Agustina; Irma Josefina Savitri; Novita Kusuma Wardhani; Michelle Suhartono; Agung Krismariono
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 2 (2023)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v4i2.3294

Kota Pasuruan merupakan salah satu kota yang masih ditemukan kasus stunting. Adanya Pandemi COVID-19 turut memberikan dampat negatif pada status stunting di Kota Pasuruan yang pada tahun 2021 tercatat sebanyak 23 persen dari tahun sebelumnya sekitar 19 persen. Salah satu sasaran primer pencegahan stunting adalah ibu hamil. Pada ibu hamil, kesehatan rongga mulut berperan besar terhadap kesehatan secara umum, termasuk untuk pencegahan stunting pada calon buah hatinya. Pada pengabdian masyarakat ini, dilakukan pengabdian kepada masyarakat dalam upaya mencegah stunting melalui pencegahan kelahiran Bayi dengan Berat Badan Lahir Rendah (BBLR) dengan cara meningkatkan pencegahan kelainan didalam mulut pada ibu hamil melalui pelatihan deteksi dini dan pencegahan kelainan periodontal pada ibu hamil dan kader kesehatan sebagai upaya pencegahan stunting di Puskesmas Kandangsapi Kota Pasuruan. Tahapan pengabdian dilakukan meliputi tahap persiapan kegiatan, mengorganisasi kegiatan, tahap edukasi dan pelatihan target sasaran, tahap pembentukan kepemimpinan, koodinasi intersectoral dan tahap akhir evaluasi kegiatan. Peningkatan pengetahuan target sasaran menjadi indikator kuantitatif keberhasilan program yang dievaluasi melalui tes tulis sebelum dan setelah pengabdian dilaksanakan. Kegiatan pengabdian masyarakat dapat meningkatkan kemampuan kognitif maupun psikomotor ibu hamil dan kader kesehatan untuk melakukan deteksi dini dan pencegahan kelainan periodontal sebagai upaya pencegahan stunting.

Kesiapan Dokter Gigi Anggota PDGI Kota Pasuruan dalam pemberian Host Modulation Therapy sebagai Terapi Penunjang Penyakit Periodontal Irma Josefina Savitri; Noer Ulfah; I Komang Evan Wijaksana; Eka Fitria Augustina; Gilang Rasuna Sabdho Wening; Erza Nudya Elwardah; Imanuel Jeremias Laturiuw
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 4 (2023)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v4i4.6336

Periodontitis, keradangan kronis jaringan penyangga gigi atau periodontal, adalah hasil interaksi dari respon imun tubuh manusia dan bakteri penyebab. Prevalensi periodontitis masih tinggi, mengganggu sistemik dan kualitas hidup, sehingga menjadikan periodontitis sebagai salah satu permasalahan gigi dan mulut yang utama. Selain terapi lokal, Host Modulating Therapy (HMT) diperlukan untuk mengurangi kerusakan jaringan dengan memodulasi respon tubuh manusia. Departemen Periodonsia FKG Unair melaksanakan pengabdian masyarakat berupa seminar HMT dengan sasaran dokter gigi di Kota Pasuruan. Tujuan penulisan artikel adalah melaporkan kesiapan dokter gigi anggota PDGI Kota Pasuruan dalam pemberian HMT sebagai terapi penunjang penyakit periodontal. Metode yang digunakan diawali dengan pemberian materi dan dilanjutkan dengan Focus Group Discussion (FGD). Pengukuran tingkat keberhasilan capaian menggunakan uji korelasi “Drug Prescribing Belief Model” dengan metode “post-test” only. Nilai evaluasi akhir didapatkan rerata peserta adalah 82 yang mengindikasikan bahwa penyampaian materi HMT telah optimal dilakukan oleh penyaji. Walaupun tingkat pengetahuan tinggi, uji korelasi Drug Prescribing Belief Model menunjukkan bahwa nilai “self efficacy” peserta rendah. Kesimpulan yang diberikan adalah dokter gigi di Kota Pasuruan memiliki pengetahuan yang baik dalam pemberian HMT namun kurang memiliki keterampilan dalam standar operasional pemberian HMT. Dari kesimpulan ini disarankan untuk dilakukan pelatihan berkelanjutan dalam hal teknis pemberian HMT

Pemeriksaan dan tindakan pencegahan sederhana untuk anak dengan Autistic Spectrum Disorder pada center pendidikan anak berkebutuhan khusus di Surabaya Savitri, Irma Josefina; Udijanto Tedjosasongko; Tania Saskianti; Gilang Rasuna Sabdho Wening; Dimas Prasetianto Wicaksono; Sindy Cornelia Nelwan; Dewina Marsha Larasati; Salwa Alya Putri; Rafie Wahyu Purnomo; Savitri, Agil Torresia Nirwanasari
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 2 (2025)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v6i2.11786

Abstract Background: ASD is at greater risk of caries than typical individuals, due to motor impairment, bad habits, sensory overload, and diet. Pits and fissures, small depressions, and ditches on the teeth's surface often become area where food remains are trapped, leading to tooth cavities. Pit and fissure sealant prevent cavities by making the pits and fissures shallow and preventing food debris from being trapped. Objectives: The aim of community service is dental examinations and pit and fissure sealant procedures for ASD children. Methods: Coordinating partners, and creating visual instructions to understand activities and reduce anxiety levels during treatment were conducted, as well as surveying experiences with dentists. Results: Videos was very helpful in preparing to go to the dentist because as many as 64% of the survey targets had no experience. The reasons were concerns about bringing children (40%), costs (40%), and difficulty finding service providers (20%). As many as 36% of surveyors had been to the dentist in the last 6 – 12 months (75%) with the main complaint being toothache and gum pain (64%) or broken teeth (36%). Most participants in community service activities were female (82%). Conclussion: Good collaboration between parents and dentists help successful treatment in ASD Keywords: Autistic Spectrum Disorder, pit dan fissure sealant, caries Abstrak Latar Belakang: ASD beresiko lebih besar menderita karies dibandingkan individu tipikal, karena hambatan motorik, kebiasaan buruk, kewalahan sensori, diet makanan. Pit dan fissure, cekungan dan parit-parit kecil di permukaan gigi, sering menjadi tempat terperangkapnya sisa makanan dan berlanjut menjadi lubang gigi. Pit dan fissure sealant adalah pencegahan gigi berlubang dengan cara pengisian pit dan fissure sehingga menjadi dangkal, dan mencegah terperangkapnya sisa-sisa makanan. Tujuan: Memberikan pemeriksaan gigi dan tindakan pit dan fissure sealant pada anak-anak penyandang ASD. Metode: Kegiatan diawali dengan koordinasi mitra pelaksana dan mitra sasaran, pembuatan instruksi secara visual untuk pemahaman kegiatan dan menurunkan tingkat kecemasan pada saat dilakukan perawatan, serta survey pengalaman ke dokter gigi. Hasil: Video sangat membantu persiapan ke dokter gigi, karena sebanyak 64% sasaran survey belum berpengalaman pergi ke dokter gigi. Kendala yang dihadapi adalah kekhawatiran membawa anak (40%), biaya (40%) dan kesulitan mencari penyedia layanan (20%). Sebanyak 36% surveyor pernah ke dokter gigi dalam kurun waktu 6 – 12 bulan terakhir (75%) dengan keluhan utama adalah sakit gigi-gusi (64%) atau gigi yang patah (36%). Peserta kegiatan pengmas sebagian besar adalah laki-laki (82%). Kesimpulan: Kerjasama yang baik antara orang tua dan dokter gigi akan membantu keberhasilan perawatan pada penyandang ASD Kata Kunci: Autistic Spectrum Disorder, pit dan fissure sealant, karies

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