Nanik Zubaidah
Department Of Conservative Dentistry Faculty Of Dental Medicine, Universitas Airlangga

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Successful Single Visit Endodontic Using Bioceramic Sealer and Esthetic Restoration with Porcelain Fused to Zirconia: A Case Report Meidi Kurnia Ariani; Nanik Zubaidah
Health Notions Vol 6, No 6 (2022): June
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60607

Abstract

Background: Single visit endodontic therapy may be defined as the conservative non surgical treatment of an endodontically involved tooth, consisting of complete chemomechanical instrumentation and obturation of the root canal system accomplished in one visit. Case: A 35-year-old woman comes with chief complaint on her upper front left tooth. Clinical examination revealed that the tooth #22 had deep caries dan reached the pulp, non vital teeth. Periapical radiolograph showed radiolucent area in the apical area of tooth #22. The diagnosis is necrosis of pulp with asymptomatic apical periodontitis. Case Management: Tooth #22 was treated with single visit root canal treatment using bioceramic sealer followed by fiber post cementation and porcelain fused to zirconia crown. Patients had no longer complain of pain after treatment. Conclusion: Single visit endodontic can successfully eliminate patient complaints and restore tooth function. Keywords: single visit endodontic; bioceramic sealer; porcelain fused to zirconia
Degradation of Fusobacterium nucleatum biofilm and quantity of reactive oxygen species due to a combination of photodynamic therapy and 2.5% sodium hypochlorite Nanik Zubaidah; Sukaton; Sri Kunarti; Meidi Kurnia Ariani; Dawailatur Rahman Setiady; Dur Muhammad Lashari
Dental Journal Vol. 56 No. 2 (2023): June 2023
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i2.p132-138

Abstract

Background: The persistence of microorganisms in the root canal system is one of the leading causes of root canal treatment failure. Biofilms of putative pathogens hidden inside dentin tubules and other root canal ramifications may limit current disinfection protocols. Photodynamic therapy (PDT) with a wavelength of 628 nm can be used as an antimicrobial strategy that uses low-power laser energy to activate a non-toxic photosensitizer to produce singlet oxygen with the ability to kill microorganisms in root canals. Fusobacterium nucleatum was used because this bacterium is one of the bacteria involved in root canal infection. Purpose: The aim of this study was to compare the bactericidal efficacy of sodium hypochlorite (NaOCl) 2.5%, PDT, and a combination of PDT and NaOCl 2.5% against Fusobacterium nucleatum. Methods: Mature biofilm Fusobacterium nucleatum was divided into four groups according to the protocol of decontamination: K1 (negative control – biofilm), K2 (NaOCl 2.5%), K3 (PDT), and K4 (NaOCl 2.5% + PDT). Biofilm degradation was observed using optical density (OD) at 570 nm using a microplate reader. A reactive oxygen species quantity check was carried out using a nitroblue tetrazolium test, and OD observation was done with a microplate reader at 540 nm. Results: Group 4 (NaOCl 2.5% + PDT) showed more biofilm bacteria elimination than the other groups. Conclusion: A combination of PDT and NaOCl 2.5% can be considered an effective protocol for the elimination of Fusobacterium nucleatum. There is a potentiation relationship between NaOCl 2.5% and PDT FotoSan. Biofilm degradation occurs because of the effect of antibacterial NaOCl 2.5% and the irradiation effect of the Toluidine blue O photosensitizer.
The effect addition of epigallocatechin-3-gallate (EGCG) in nano hydroxyapatite on surface porosity as a candidate pulp capping materia Ismiyatin, Kun; Zubaidah, Nanik; Azzaria, Indira Moza
Conservative Dentistry Journal Vol. 13 No. 1 (2023): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i1.2023.24-28

Abstract

Background: Deep caries, cavity preparation and use of burs or other dental instruments often result in pulpal perforation. In the case of an exposed pulp, regenerative pulp tissue treatment aims to regenerate normal tissue and maintain pulp vitality by inducing pulp regeneration using the right material, so that a good percentage and size of material porosity is needed to help cell regeneration by supporting cell proliferation and attachment, stimulating remineralization and differentiation of odontoblast-like cells. Nano-HA has the ability to produce dentine bridges continuously, has porous properties that allow cell growth, improves mechanical properties, but is not anti-inflammatory so that EGCG is added which has the advantage of being an antioxidant, anti-inflammatory which can optimize pulp tissue regeneration and also acts as an antimicrobial by reduce the growth of bacteria in the oral cavity and can trigger the proliferation and differentiation of human dental pulp cells. Purpose: This study aims to prove the difference in surface porosity of nano hydroxyapatite added with EGCG compared to nano hydroxyapatite and aquadest. Methods: This study used a laboratory experimental study with a posttest-only control group design. The research sample consisted of 32 samples which were divided into 2 groups, the treatment group (nano HA - EGCG) and the control group (nano HA - aquadest). Each research group was subjected to freeze drying and SEM tests. Results: There was a significant difference in the percentage of surface porosity between the nano hydroxyapatite added with EGCG compared to nano hydroxyapatite and aquadest.on the results of the Independent T-test (p<0.05). Conclusion: The addition of EGCG to nano hydroxyapatite has a higher porosity percentage compared to nano hydroxyapatite with aquadest.
The potency of propolis extract as anti-inflammatory agent for stimulation of reparative dentin formation Anindya, Cinitra; Widjiastuti, Ira; Zubaidah, Nanik
Conservative Dentistry Journal Vol. 13 No. 1 (2023): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i1.2023.42-47

Abstract

Background: As an effort to treat a deep caries lesion with a diagnosis of reversible pulpitis, one of the treatment options is pulp capping. For decades, calcium hydroxide has been considered the most acceptable pulp capping material and has been routinely used in dental practice, but it also has some weakness. To overcome that, alternative materials are needed that can provide a better effect. Recently, a lot of research has been done using natural ingredients, including Propolis. Propolis has been known to have strong antimicrobial and anti inflammatory properties. Therefore, it is necessary to conduct a deeper study regarding the potency of propolis extract as an anti inflammatory agent for the stimulation of reparative dentin formation so that it can be considered to be used as a pulp capping material. Objective: The aim of this literature review is to highlight the potency of propolis extract as anti inflammatory agent in the natural stimulation of reparative dentin formation. Methods: The search strategy consist of an electronic research using databases such as PubMed, Google Scholar, Science Direct, dan Springer Link for studies published from 2003 to 2021 regarding the potency of propolis extract as anti inflammatory agent for stimulation of reparative dentin formation, including review articles and research articles. Results: Propolis and its constituents have been proven to be used as a potent natural anti inflammatory, it also considered to have the ability to promote reparative dentin formation. However, there are some literatures stated that reparative dentin does not form with the application of propolis, and the causes may vary. Conclusion: Propolis extract has a potency as an anti inflammatory agent in dental pulp inflammation by inhibiting TNFα expression, and increasing TGF-β1 expression, as well as its potency in stimulating the formation of reparative dentin. Based on this review, further studies are needed.
Effects of hydroxyapatite bovine tooth graft (HAp-BTG) and polyethylene glycol (PEG) combinations in post extraction sockets on the amount of osteoid Zubaidah, Nanik; Wulandini, Raden Ajeng; Sa'adah, Nur Hikmatus; Arrianti, Tyas Ramadhini; Pribadi, Nirawati
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.56-59

Abstract

Background: One of the actions in the field of tooth conservation such as hemisection can result in changes, especially in hard tissue due to the extraction of tooth roots and part of the tooth crown. After tooth extraction, socket wound healing will occur which ends with the process of osteogenesis. This process can reduce the dimensions of the socket due to alveolar bone resorption. Socket preservation can prevent dimension reduction and bone resorption to achieve maximum treatment results. The administration of Hydroxyapatite bovine tooth graft (HAp-BTG) into the post-extraction socket is expected to increase the formation of osteoid matrix which is important in the formation of new bone. Purpose: Proving the effect of adding a combination of HAp-BTG and PEG into the post-extraction socket on increasing the number of osteoid. Methods: 32 wistar rats were divided into control and treatment groups. Then the lower left incisor was extracted, the post-extraction socket was filled with PEG for the control group and a combination of HAp-BTG and PEG for the treatment group. On the 14th and 28th day the wistar rats were terminated and the mandibles were taken to make tissue preparations. HE staining was performed on the samples and observing the extent of the osteoid using a microscope with 400x magnification. Results: There was a significant difference between the control group and the treatment group on the 14th and 28th days. Conclusion: Administration of a combination of HAp-BTG and PEG into the post-extraction socket increased the number of osteoid on the 14th and 28th days.
BMP-7 Expression After Induction of Hydroxyapatite Bovine Tooth Graft Gel as A Socket Preservation of The Alveolar Bone (Laboratory Experimental of The Alveolar Bone) Zubaidah, Nanik; Namira, Safa Marety; Aryandhita, Amanda Paramitha Putri; Firyasasty, Beffano Roziq Herdymunas; Cahyani, Febriastuti
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.60-63

Abstract

Background: Hemisection is an endodontic surgical procedure that can leave alveolar bone defects. Changes in the dimensions of the alveolar bone can be minimized by preserving the alveolar bone socket using a bone graft. Induction of Hydroxyapatite Bovine Tooth Graft (HAp-BTG) in the alveolar bone socket is expected to increase the expression of BMP-7 which is a growth factor in osteogenesis. Purpose: To describe the increase in BMP-7 expression after induction of hydroxyapatite bovine tooth graft gel as a socket preservation of the alveolar bone of Wistar rats on the 14th and 28th days. Methods: 28 Wistar rats were extracted on the lower left incisor. Post-extraction sockets were induced by Polyethylene Glycol (PEG) as the control group and HAp-BTG + PEG as the treatment group. On the 14th and 28th day the Wistar rats were euthanized and the mandibles were taken. By using immunohistochemical staining on anatomic histopathological examination, BMP-7 expression was observed using a microscope with 1000í— magnification. The research results were analyzed using One Way ANOVA. Results: There was a significant increase in BMP-7 expression between the control group and the treatment group. Conclusion: BMP-7 expression after induction of hydroxyapatite bovine tooth graft gel as a socket preservation of the alveolar bone of Wistar rats increased on the 14th and 28th days.
Apexification with mineral trioxide aggregate on post trauma right maxillary central incivus teeth Masa, Maria Margaretha S. Nogo; Pratiwi, Dian Dwi; Cahyani, Febriastuti; Zubaidah, Nanik; Namira, Safa Marety; Wulandini, Raden Ajeng; Tanzil, Muhammad Iqbal; Oktaria, Wanda; Saraswati, Widya
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.81-84

Abstract

Background: Trauma often occurs in the maxillary anterior teeth in both children and adults. This usually causes the pulp of a tooth whose root is not yet fully formed to experience necrosis, and the apex closure stops causing the apex to be wide and open. Apexification is a method to induce a calcified barrier in necrotic roots with open apex. Treatment with Mineral trioxide aggregate (MTA) can shorten treatment time and has a higher long-term success rate than using Ca(OH)2. Purpose: The aim of this report is to describe apexification cases using MTA on a non-vital right maxillary central incisor. Case: A 21-year-old male patient came to the Dental Conservation Clinic at Airlangga University Dental Hospital with complaints of broken and discolored front teeth. The tooth was broken after falling ± 10 years ago (2013) while playing football, it was swollen and painful, but in the last ± 1 year (2022) it has never hurt again. The patient's medical history revealed no systemic abnormalities. Objective examination of tooth 11 class IV ellis fracture, negative percussion and bite test, surrounding normal gingiva, no mobility. The radiographic appearance of tooth 11 shows a wide open apex and a radiolucency at the root tip. Vitality of non-vital teeth, Diagnosis of pulpal necrosis with asymptomatic apical periodontitis. Case Management: In this case, the treatment plan is endodontic treatment in the form of apexification with MTA material. The obturation technique was performed using warm thermoplastic (backfill) and accompanied by a fixed restoration using a fiber post and all porcelain crown with a good prognosis for patient care. Conclusion: Apexification with MTA with post and IPS Emax crown restoration is a good treatment option for immature right maxillary central incisor with open apex. The patient is satisfied because the teeth can return both aesthetically and functionally according to the stomatognathic system.
In office external bleaching of teeth with discoloration: a case report Anindya, Cinitra; Ismiyatin, Kun; Zubaidah, Nanik; Supandji, Rafaelle Raditya; Nurkhalidah, Binar Najwa
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.6-10

Abstract

Background: Tooth discoloration is a prevalent issue that significantly impacts an individual's self-confidence. Fortunately, dentists offer highly effective and non-invasive teeth whitening techniques that use higher concentrations of hydrogen peroxide (HP) of around 35% to 40%. These techniques can help resolve the problem of teeth discoloration better than at-home methods designed to deliver faster results. This is particularly helpful when immediate whitening is required. Purpose: This case study aims to report cases of in-office external bleaching of discolored teeth. Case: A 29-year-old woman complained about the yellowish shade of her teeth, which made her feel less confident due to the yellowish color. Case Management: The management for discoloration of teeth was done with dental bleaching using 40% hydrogen peroxide. As a result of the treatment, the desired tooth color increased from 3M1 to 1M1 Vita 3D Master and from Opalescence no.6 to 4. Conclusion: In-office dental bleaching is a highly effective treatment option for correcting tooth discoloration that dental professionals widely use. The procedure can significantly improve the appearance of discolored teeth and increase self-confidence of the patient.
Apex resection treatment on tooth 21 and 22 with suspected radicular cyst Cahyani, Febriastuti; Saraswati, Widya; Mulyadi, Reyz Pasenda; Wahab, Sofi Arnesti; Halima, Siti Noorraida; Dzulfikri, Ahzahra Indah; Zubaidah, Nanik; Kunarti, Sri; Soetojo, Adioro
Conservative Dentistry Journal Vol. 14 No. 2 (2024): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i2.2024.87-89

Abstract

Background: Apex resection is the surgical removal of the apex or root end of a tooth. Endodontic failure is the most usual reason of treatment being performed. Retreatment may be the first option for teeth which have been treated conventionally and exhibit a persistent apical lesion. In particular, failures during conventional therapy could hinder results by leading infections to spread to inaccessible apical regions, necessitating surgery. Purpose: This case report aims to describe the management of radicular cyst on previously treated tooth. Case: A 51-year-old male patient came to Soewandhie General Hospital with a complaint of swelling on his hard palate from one year before. The left maxillary incisor has undergone root canal treatment about 1 year ago, but had not been permanently filled. Case Management: The treatments given were retreatment for 21 and root canal treatment for 22, then followed by cyst enucleation and apex resection on both teeth. Conclusion: Apex resection shows a satisfying result for treating previously treated tooth with persistent apical lesion.
Antibiofilm activity of probiotic bacteria Lactobacillus plantarum FNCC 0020 against Streptococcus mutans serotype c Pribadi, Nirawati; Sampoerno, Galih; Juniarti, Devi Eka; Goenharto, Setyabudi; Zubaidah, Nanik; Ramadhini Arrianti, Tyas; Ramadhani, Aqila Shabrina Dwi; Fadhillah, Revita Rizki
Conservative Dentistry Journal Vol. 15 No. 1 (2025): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i1.2025.33-37

Abstract

Background: Streptococcus mutans (S. mutans) is a Gram-positive bacterium that plays a role in dental caries. Plaque and biofilm formation can be chemically cleaned using mouthwash, such as chlorhexidine gluconates (CHX) 0.2%, which is the gold standard. Another alternative can be developed to inhibit S. mutans biofilm formation by using Lactobacillus plantarum probiotic, which has antibiofilm potency by producing antimicrobial substances. Purpose: This study tested the biofilm inhibition to determine the Inhibitory Concentration that can inhibit 50% of biofilm formation. Methods: This research was conducted as an in vitro experimental laboratory study. Biofilm inhibition testing was performed by using the microdilution method on biofilms formed in 96 well microplates with 0,1% crystal violet staining. Lactobacillus plantarum FNCC 0020 was diluted to several concentrations: 12.5%, 25%, and 50% against Streptococcus mutans biofilm induced by 3% sucrose. The test results in optical density were read using a spectrophotometry with a wavelength of 650 nm. were determined using the GraphPad Prism sigmoidal dose-response method. Results: There is antibiofilm activity of Lactobacillus plantarum FNCC 0020 against Streptococcus mutans, and the inhibitory effect against Streptococcus mutans biofilm showed of 42.43%. Conclusion: Lactobacillus plantarum FNCC 0020 at 50% has biofilm inhibitory activity compared to other concentrations but is less effective than 0.2% CHX.
Co-Authors Adioro Soetojo Agnes Melinda Wong Agus Subiwahjudi Ahmad Afif Dzulfikar Akhmad Arif Musadad, Akhmad Andi Kurniawan Arrianti, Tyas Ramadhini Aryandhita, Amanda Paramitha Putri Azzaria, Indira Moza Cindy Ramadhan Putri Cinitra Anindya Daradjat, Kinanti Naianuha Dawailatur Rahman Setiady Devi Eka Juniarti Dian Dwi Pratiwi Dinda Dewi Artini Dur Muhammad Lashari Dzulfikri, Ahzahra Indah Fadhillah, Revita Rizki Febriastuti Cahyani Firyasasty, Beffano Roziq Herdymunas Firza Basalamah Galih Sampoerno Galih Sampoerno Goenharto, Setyabudi Gusniarti, Haninda Dhipta Halima, Siti Noorraida Hasna Shabrina Hendri Eko Wahyudi Ira Widjiastuti Ivan Nur Fadela Jasmina Putri Azizia Jesica Ceren Karina Erda Saninggar Karina Erda Saninggar Ketut Suardita, Ketut Kun Ismiyatin Maretha Siwinata Masa, Maria Margaretha S. Nogo Meidi Kurnia Ariani Meidi Kurnia Ariani Mifta Izha A R Moch. Mudjiono Muhammad Dimas Aditya Ari Mulyadi, Reyz Pasenda Nadira Nurin Febrianti Namira, Safa Marety Nindhira Puspita Sari Nirawati Pribadi Nurkhalidah, Binar Najwa Oktaria, Wanda Pratiwi, Dian Dwi Radixtio Auzan Fepiosandi Ramadhani, Aqila Shabrina Dwi Ramadhini Arrianti, Tyas Ramdhan Hanif Nahada Ratih Mutiara Sani Sa'adah, Nur Hikmatus Sandra Kartika Sari Singgih Harseno Sri Kunarti Sri Kunarti, Sri Sukaton, Sukaton Supandji, Rafaelle Raditya Sylvia Tamara Nitya A Tamara Yuanita Tanzil, Muhammad Iqbal Wahab, Sofi Arnesti Wanda Salsabilla Widya Saraswati Wulandini, Raden Ajeng yanto, Sudi Yasmin Safira Virgiani Yosefin Adventa