Tamara Yuanita
Conservative Dentistry Department, Faculty Of Dentistry, Universitas Airlangga,

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Journal : Conservative Dentistry Journal

DAYA ANTI BAKTERI EKSTRAK KULIT NANAS (Ananas comosus) TERHADAP PERTUMBUHAN BAKTERI Enterococcus faecalis ANTIBACTERIAL POTENCY OF PINEAPPLE PEEL EXTRACT (Ananas comosus) ON Enterococcus faecalis GROWTH Rega Maurischa Arantika Putri; Tamara Yuanita; Mohammad Roelianto
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.736 KB) | DOI: 10.20473/cdj.v6i2.2016.61-65

Abstract

Background: Root canal infections is caused by the colonization of microorganisms. Microorganisms that commonly found in root canal failure is Enterococcus faecalis. These bacteria can be found in 80-90% of root canal infections. Currently, many plants are used as antibacterial drugs. Pineapple is one plant that can be used as antibacterial drugs. Pineapple peel has a variety of antibacterial chemical compounds, such as, bromelain, saponins, tannins and flavonoids that can inhibit the growth of Enterococcus faecalis bacteria. Aim: To obtain concentration of antibacterial from pineapple peel extract on Enterococcus faecalis growth. Methods: This study was an experimental laboratory with Post Test Only control group design using Enterococcus faecalis ATCC 29212 bacteria that were diluted based on Mc. Farland standard 1.5 x 108 CFU / ml with pineapple peel extract treatment concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0, 78% and then planted in nutrient agar media surface evenly. Results: At concentration of 3.125% pineapple peel extract showed that the growth of colonies was less than 90% of positive control and concentration of 6.25% pineapple peel extract had no visible Enterococcus faecalis bacteria growth as much as 99.9%. Conclusion: The pineapple peel extract (Ananas comosus) have antibacterial power on the growth of Enterococcus faecalis bacterial colonies with Minimal Inhibitory Concentration (MIC) of 3.125% and the Minimum Bactericidal Concentration (MBC) of 6.25%.
Perbedaan Kebocoran Tepi antara GIC Konvensional dan Resin Modified GIC pada Restorasi Kelas V (Difference on Microleakage of Conventional GIC and Resin Modified GIC in Class V Restoration) Aditya Rama Devara; Cecilia G.J Lunardhi; Tamara Yuanita
Conservative Dentistry Journal Vol. 6 No. 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.276 KB) | DOI: 10.20473/cdj.v6i2.2016.77-81

Abstract

Background. Microleakage is one of the challenging concerns in direct filling restorations. Restoration material should have good adaptation between the restoration and the cavity walls to seal the cavity in a good way. Glass ionomer cement (GIC) is one of restoration material which indicated for class V cavity. GIC has a good adherence to the cavity wall but fragile to liquid contamination during the setting time. Resin modified glass ionomer cement (RMGIC) is a hybrid glass ionomer cement with the addition of hidroxyethylmethacrylate (HEMA) in the liquid to increase the properties and endurance to liquid contamination. Aim. The aim of this study was to investigate the difference on microleakage of conventional GIC and resin modified GIC in class V restoration. Material and methods. Class V cavities (depth: 2 mm) were prepared on the cervical surface of  32 human first permanent  premolars. Teeth were classified into three groups. Group 1: conventional GIC + varnish. Group 2:RMGIC + varnish. All cavities were restored, then stored in artificial saliva at 37ºC for 24 hours. The teeth were immersed in a 1% methylene blue dye solution for 24 hours, and then rinsed in running water, dried, and sectioned longitudinally. The section were assessed for microleakage of dye penetration by two independent evaluators using a digital microscope. Data were collected and statistically analyzed. Results. RMGIC showed no significant difference with conventional GIC. However, there is a slight difference, RMGIC has a slight lower microleakage than conventional GIC. Conclusion. RMGIC showed only slight lower microleakage than conventional GIC, but not significant.
EKSPRESI Interleukin-1 (IL-1) PADA PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis Yuliana Dwiwahyu Suryandari; Ketut Suardita; M. Mudjiono; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.196 KB) | DOI: 10.20473/cdj.v7i2.2017.59-65

Abstract

Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-1. IL-1 is the proinflammation cytokine that is the key of host response bacteria invation and tissue damage. Also IL-1 could cause some indirectly tissue damage through the activation of MMPS. MMPs to stop the collagen formation. Purpose. The aim of this study is to know about the expression of IL- 1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of IL-1 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of IL-1 is increasing during the periapical tissue damage that induced by E.faecalis.
EKSPRESI Tumor Necrosis Factor alpha (TNF-α) DAN Transforming growth factors beta (TGF-β) PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI Enterococcus faecalis PADA TIKUS WISTAR Richard Fritzgerald; Cecilia lunardhi; Ruslan Effendy; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.733 KB) | DOI: 10.20473/cdj.v7i2.2017.66-73

Abstract

Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation then lead to bone destruction. LTA stimulates immunology reaction that produce Tumor Necrosis Factor alpha (TNF-α) and Transforming growth factors beta (TGF-ß). TNF-α is a main mediator and also have an important role in inflamation response otherwise TGF-ß is working as a multifunction  regulator of cell growth and differentiation during reforming and remodelling.  Purpose. The aim of this study is to know about the expression of TNF-α and TGF-ß during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 30 male rats were randomly divided into 3 main groups, Group A (control negative) : normal tooth. Group B (control positive) : every tooth was induced only by sterile BHI-b. Group C (treated group) : every tooth  was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU). The animals were sacrificed 21 days later and prepared for histological examination of tissue damage, then we did the immunohistochemistry  followed by calculation on the light microscope. Result. The analysis revealed that the expression of TNF-α at treated group are higher than negative control and positive control but the expression of  TGF-ß at treated group are higher than the negative control group but lower than positive control. Conclusion. From this study we know that the expression of TNF-α and TGF-ß are changing during the periapical tissue damage that induced by E.faecalis.
EKSPRESI TNF-α DAN CALCINEURIN PADA ASIMTOMATIS APIKAL PERIODONTITIS AKIBAT INDUKSI Enterococcus faecalis Randy Carlos Sietho; Mandojo Rukmo; Edhie Arif Prasetyo; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.779 KB) | DOI: 10.20473/cdj.v7i2.2017.74-85

Abstract

Background. Gram positive bacteria strain are the major cause of endodontic failure as asymptomatic apical periodontitis. One of the dominant group of bacteria is Enterococcus faecalis that still persistent in root canal system post endodontic therapy procedures. This bacteria has lipoteichoic acid on its membrane that can cause induction of cytokines expression such as Tumor Necrosing Factor-α (TNF-α) and Calcineurin Purpose. This experiment to demonstrated asymptomatic apical periodontitis that induced with Enteroccus faecalis produce raising amount of TNF-α and Calcineurin expression cells in pericapical tissue of wistar rat. Method. The upper right molar teeth of the rat was drilled until perforation then exposed by BHIB 10µl (control positive group), E.faecalis 106 CFU in BHIB 10µl (experimental group) and without drilling (control negative group) then observed until 21th days and counting the amount of TNF-α and Calcineurin expression cells. Conclusion.The results show that asymtomatic apical periodontitis that was induced E.faecalis produce increasing amount of TNF-α and Calcineurin expression cells in periapical tissue wistar rat.
EKSPRESI TGF-B DAN MMP-1 PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS TIKUS WISTAR Tamara Yuanita; Hadriany Hotmaria; Ruslan Effendy; Ketut Suardita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.913 KB) | DOI: 10.20473/cdj.v7i2.2017.86-94

Abstract

Background. The main etiology of endodontic treatment failure  is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of TGF-ß to enhance the proliferation collagen and MMP-1 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of of TGF-ß and MMP-1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 27 male rats were randomly divided into 3 main groups. Group A (negative control) : every tooth was’nt induced by anything. Group B ( positive control): every tooth was induced only by sterile BHIb and closed by GIC Fuji II as the final restoration. Group C (: every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), and closed by GIC Fuji II as the final restoration. The animals were sacrificed after 21 days and prepared for histological examination of tissue damage, then we did the immunohistochemistry  followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-1 increased significantly in group C when E.faecalis was induced. When expression of TGF-ß decreaced significantly in group C rather than group B.  Conclusion. From this study we know that the expression of TGF-ß and MMP-1 are make opposite pathway due to chronic apical periodontitis that induced by E.faecalis.
EKSPRESI MATRIKS METALLOPROTEINASE-8 DAN INTERLEUKIN-8 PADA KERUSAKAN JARINGAN PERIAPIKAL AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS Tamara Yuanita; Tantri Wismayaning Radito; Dian Agustin wahjuningrum; R. Roulianto
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.637 KB) | DOI: 10.20473/cdj.v7i2.2017.95-101

Abstract

Background. The main etiology of endodontic treatment failure  is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-8 to enhance the inflamation reaction and MMP-8 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of MMP-8 and IL-8 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry  followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-8 and IL-8 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of MMP-8 and IL-8 are increasing during the periapical tissue damage that induced by E.faecalis.
EKSPRESI Nuclear Factor of Activated T cells c-1 (NFATc-1) DAN OSTEOKALSIN PADA KERUSAKAN TULANG PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis Arindah Hadi; M. Roelianto; Ari Subiyanto; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.617 KB) | DOI: 10.20473/cdj.v7i2.2017.138-144

Abstract

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation then lead to bone destruction. Bone destruction occurs due to the inflammation process that is mediated by immune system. The important cell in the process of bone destruction is osteoclast. Bone destruction is marked by the form of osteoclast that is called osteoclastogenesis. NFATc-1 and osteocalcin play important things in osteoclastogenesis. Purpose. The aim of this study is to know about the expression of NFATc-1 and osteocalcin during the periapical bone destruction due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of periapical bone, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of NFATc-1 and osteoclast increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of NFATc-1 and osteocalcin are increasing during the periapical bone destruction that induced by E.faecalis.
Perbedaan Daya AntibakteriEkstrak Kulit Kokoa (Theobroma cacao)dan NaOCl 2,5% terhadapPorphyromonas gingivalis Tamara Yuanita; Rifatul Jannah; Edhie Arif Pasetyo; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.693 KB) | DOI: 10.20473/cdj.v8i1.2018.49-56

Abstract

Background: Since pulp infection plays an important role in the development of periradicular lesions, endodontic treatment should be directed to eliminate bacterial and theirproducts. However, currently 20% of cases of apical periodontitis are not resolved after root canal treatment and therefore required for new root canal disinfection. The most commonly used irrigation material today is NaOCl 2.5%. However, NaOCl has negative effects, including being toxic when the material is injected into the periradicular tissue causing extensive pain, bleeding and swelling. Until now, many drugs come from plants that are still produced from plant extracts. One of the plants that can be utilized is cocoa (Theobroma cacao). Cocoa contains active compounds, such as saponins, tannins, alkaloids, flanonoids, aromatic terpenoids, theobromins and other metabolites. Cocoa husk has been studied to have an antibacterial effect on Porphyromonas gingivalis which is the main bacterial cause of apical periodontal. However, the difference in antibacterial activity between cocoa husk extract and NaOCl 2.5%  againstPorphyromonas gingivalis has not been studied. Porpuse:The aim of this study is to compare antibacterial activity of cocoa husk extract and NaOCl 2.5% againstPorphyromonas gingivalis.Method: This research was a laboratory experimental study. Porphyromonas gingivalis were swabbed to nutrient agar medium. Consequently, cocoa husk extract 25% and NaOCl 2.5% were placed in wells of 5mm diameter and nutrient agar medium. The diameter of the zone of inhibition around the test materials was measured after 24 hours.Result:Cocoa husk extract has lower mean inhibitory zone diameter (14.22) than NaOCl 2.5% (16.06). Conclusion:Cocoa husk extract has lower antibacterial activity against Porphyromonas gingivalis compared to NaOCl 2.5%.
Antibakteri Ekstrak Kulit Buah Kakao (Theobroma cacao) 6,25% dan NaOCl 2,5% Terhadap Bakteri Streptococcus sanguinis Sepdhyo Wahyu Nugroho; Mandojo Rukmo; Edhie Arief Prasetyo; Tamara Yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (648.657 KB) | DOI: 10.20473/cdj.v9i1.2019.19-21

Abstract

Background: Streptococcus sanguinis is a gram-positive bacterium that infects and penetrates into dentinal tubules from a depth of 150 μm up to 792 μm. Chemicals, namely 2.5% NaOCl, are used to mitigate the growth of these bacteria via irrigation of the root canals, but 2.5% NaOCl still has disadvantages including irritating periradicular tissue, having an unpleasant odor, and being toxic. Due to these shortcomings, natural materials are expected to be used as alternatives. Cocoa peel extract has active tannin compounds, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial attributes; a concentration of 6.25% is counted as the minimum bactericidal concentration (MBC) of Streptococcus sanguinis. Aim: To compare the difference of antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) and 2.5% NaOCl against Streptococcus sanguinis. Method: This research is an in vitro experimental laboratory with post-test only control group design. The diffusion method was used on Streptococcus sanguinis planted in tubes containing BHIB, then cultured on petri dishes containing nutrient agar and divided into 3 parts, namely 6.25% cocoa peel extract, 2.5% NaOCl, and negative controls, then each Petri dishes were given a paper disc that had been dripped by 0.01 ml of each ingredient, then incubated in an incubator for 2x24 hours at 37°C; the diameter of the inhibitory zone formed was then observed using a caliper. Results: The average diameter of the inhibition zone formed on 6.25% cocoa peel extract was 19.2000 mm and 2.5% NaOCl was 17.2813 mm against Streptococcus sanguinis. Conclusion: The antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) is higher than 2.5% NaOCl against Streptococcus sanguinis.
Co-Authors Adioro Soetojo Aditya Rama Devara Afifah, Anisa Nur Agus Subiwahjudi Agus Subiwahjudi Agus Subiyanto Alissa Amanda Amalia Iqony, Rosa Anandita, Tiara Ari Subiyanto Ari Subiyanto, Ari Arindah Hadi Bin Sharizal, Shafy Shariz Cecilia G.J Lunardhi Cecilia lunardhi Chairil Hafiz, Teuku Charles, William Christabel, Phebe Fedora Deavita Dinari Devi Eka Juniarti Dian Agustin Wahjuningrum Dina Ristyawati Edhie Arief Prasetyo Edhie Arif Pasetyo Edhie Arif Prasetyo Eric Priyo Prasetyo Fajar Agus Muttaqin Fajariana Fitriani Firmansyah, Awang Bagus Galih Sampoerno Hadriany Hotmaria Ika Tangdan Jesica Ceren Karlina Samadi Ketut Suardita, Ketut Kuntaman Kuntaman Latief Mooduto, Latief Liong, Michelle M. Mudjiono M. Roelianto Mandojo Rukmo Marbun, Tarsardo Marpaung, Grace Julieta Mega Selvia Mifta Izha A R Mohammad Roelianto Mohammed Alaqsha Brysoul Ceson Nabilla, Tasya Nadien Sekar Pramesti Nanik Zubaidah Nila Murni Wulandari Nirawati Pribadi Paidal, Nurfahira Paramitha, Shafa Marwa Moza Prasetyaningtias, Silvia Arlita Hayu Prasita, Shafa Qintan Sekar Adjani R. Roulianto Randy Carlos Sietho Rega Maurischa Arantika Putri Reinold Christian Lina Revina Ester Iriani Marpaung Richard Fritzgerald Rifatul Jannah Ruslan Effendy Ruslan Effendy Salma, , Adinda Fazzahra Sepdhyo Wahyu Nugroho Setyabudi Setyabudi Setyabudi Sholeh Ardjanggi Sukandar, Wilson Sukaton, Sukaton Sylvia Paulina Panggono Tania, Cindy Grace Tantri Wismayaning Radito Tarigan, Shindyloken Juni Artha Tarsardo Marbun Teuku Chairil Hafiz Ulfadi , Bakhiitah Thufailah Putri Ulfadi, Bakhiitah Thufallah Putri Uli Sasi Andari Wiratno, Amelia Rahma Yuliana Dwiwahyu Suryandari Yulianti Kartini Sunur