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All Journal Jurnal Pengendalian Hayati AGRIKA Cropsaver : Journal of Plant Protection MAPETA Agridevina : Berkala Ilmiah Agribisnis Plumula : Berkala Ilmiah Agroteknologi Kultivasi BIOEDUSCIENCE Jurnal Pertanian Agros Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah Hijau Cendekia PEDULI: Jurnal Imiah Pengabdian Pada Masyarakat Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Agrotropika Nusantara Science and Technology Proceedings Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Tri MUJOKO
Program Studi Agroteknologi, Universitas Pembangunan Nasional ”Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Potensi Paenibacillus polymyxa pada Media Molase terhadap Penyakit Layu Akibat Fusarium sp. pada Tanaman Cabai Rawit (Capsicum frutescens L.) Nur Syiami, Aning; Suryaminarsih, Penta; Mujoko, Tri
Plumula : Berkala Ilmiah Agroteknologi Vol. 13 No. 1: Januari 2025
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v13i1.221

Abstract

Tanaman cabai rawit (Capsicum frutescens L.) adalah salah satu tanaman yang bernilai ekonomis tinggi. Produktivitas tanaman cabai rawit seringkali terhambat oleh penyakit layu Fusarium sehingga memerlukan upaya pengendalian. Paenibacillus polymyxa merupakan bakteri antagonis yang dapat mengendalikan patogen penyebab Layu Fusarium (Fusarium sp.). Penelitian bertujuan untuk mengetahui potensi P. polymyxa pada media pembawa molase terhadap penyakit layu (Fusarium sp.) tanaman cabai rawit dan mengetahui konsentrasi media pembawa molase yang efektif bagi P. polymyxa dalam mengendalikan penyakit layu (Fusarium sp.). Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan menggunakan 4 taraf perlakuan dan menggunakan 4 ulangan. Data dianalisis menggunakan analisis sidik ragam dan uji lanjut Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil uji percobaan dilakukan uji lanjutan dengan BNJ 5%. Hasil penelitian menunjukkan bahwa penggunaan P. polymyxa + molase 30% efektif dalam menghambat dan menekan infeksi Fusarium sp. pada tanaman cabai rawit.
Potential of The Biological Agent Bacillus sp. in Inhibiting Moler Disease and its Effects on The Growth and Production of Shallot (Allium ascalonicum L.) Yuwono, F. Tsara Nafisah; Wuryandari, Yenny; Mujoko, Tri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6428

Abstract

Shallot (Allium ascalonicum L.) is one of the important commodities in Indonesia. The main obstacle that is often faced is moler disease caused by Fusarium sp. Bacillus sp. bacteria have the potential to control Fusarium sp. The purpose of study was to determine the ability of Bacillus sp. in controlling moler disease and its effect on the growth and production of onion plants. The study used a factorial completely randomized design with three replications and nine treatments, so there were 27 experimental units. The observation variables included incubation period, disease intensity, plant height, number of leaves, and tuber wet weight. The research data were analyzed using the ANOVA analysis of variance procedure to determine whether there was an effect of each treatment. If it is known that there is a significantly different effect from the treatment, it will be continued with the DMRT test at the 5 % level. The results showed that the combination of Bacillus sp. isolate bcz 16 and dosage 35 ml/polybag was the longest delaying the incubation period of 15 day and could suppress moler disease by 50 %. The treatment of Bacillus sp. isolate bcz 16 and dosage 35 ml also gave the highest results in plant length, number of leaves, and wet weight of shallot bulbs
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Antagonist Test of Streptomyces spp. from Shallot Fields in Bojonegoro Against Fusarium sp. Which Causes Moler Disease In vitro Hidayati, Kiki; Mujoko, Tri; Wuryandari, Yenny
BIOEDUSCIENCE Vol 9 No 1 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/15473

Abstract

Background: Background: Shallots are a horticultural product widely consumed in Indonesia. In 2021, shallot production will decline. This is due to pest and disease attacks on plants. Fusarium sp. is a pathogen that causes the primary disease in shallots, namely moler disease, which can cause losses of up to 50%, so control activities must be carried out. This research aims to determine the potential of Streptomyces sp. bacteria from exploring shallot planting areas in Bojonegoro against Fusarium sp. fungi in vitro. Methods: This study used 15 isolates of Streptomyces sp. from exploration results tested with Fusarium sp. using the dual culture method, and negative control (only Fusarium sp.) repeated 3 times for each treatment. Observations were carried out every day for 7 days by observing the growth of Fusarium sp. hyphae and their inhibitory power. Result: The research showed that isolate S8 had the highest inhibitory power, 49.5%. Meanwhile, the one with the lowest results was isolated S1, 34.5%. From the chitinase test results, the chitinocytic index of isolate S8 was 5.2, which is in the high category. Meanwhile, the isolate S8 cellulolytic index was 4.8 and was classified as high. Conclusions: All Streptomyces sp.p isolates inhibited the growth of Fusarium sp., which was characterized by an inhibition zone compared to the control treatment.
UJI POTENSI Streptomyces spp ISOLAT MALANG UNTUK MENEKAN PERTUMBUHAN Fusarium sp PADA TANAMAN CABAI RAWIT (Capsicum frutescens L) SECARA IN VITRO Hakim, Lukman; Mujoko, Tri; Widajati, Wiludjeng
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9121

Abstract

Streptomyces spp is a group of actinomycetes bacteria that can be used as biological control agents producing antibiotics Streptomyces spp. proven by its ability to inhibit the pathogen Fusarium sp causing fusarium wilt disease in plants. This study aims to determine the potential of Streptomyces spp isolated from chili rhizosphere soil in Malang city, East Java in controlling Fusarium sp. This research was conducted in vitro at the Plant Health Laboratory 1, Faculty of Agriculture, National Development University "Veteran" East Java in November 2023 using a complete randomized design (CRD) with 10 treatments, namely Streptomyces spp isolates with 3 replications. The results of the study obtained 10 isolates of Streptomyces spp and conducted antagonistic tests with Fusarium sp. Streptomyces isolates 6 and 9 have a higher antagonistic ability compared to other Streptomyces isolates with a percentage of 48.3% and 41.9%.
EFIKASI Streptomyces sp. TERHADAP PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI MERAH (Capsicum annuum L.) DENGAN WAKTU APLIKASI YANG BERBEDA Ekayanti, Savira Aprilia; Suryaminarsih, Penta; Mujoko, Tri
Agros Journal of Agriculture Science Vol 25, No 4 (2023): edisi Oktober
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i4.3547

Abstract

Fusarium wilt disease in red pepper plants is caused by the pathogenic fungus Fusarium sp. This disease is classified as an important disease of red chili plants because it can cause damage up to 50%. One of the efforts to overcome fusarium wilt disease is to use biological agents Streptomyces sp. which comes from the field of shallots and tomatoes in Pare, Kediri. The purpose of this study was to determine the effect of the application of Streptomyces sp. Against Fusarium wilt disease in red pepper plants with a wide range of application times. This study used a Factorial Complete Randomized Design with SP and BMP isolate type treatment, as well as application time treatment before planting, at the same time planting and after planting. Each treatment was repeated 5 times and given 5 plant units on each repetition. Observation parameters were carried out on the incubation period, disease severity, plant height, number of leaves, flowering age and number of flowers. Application Streptomyces sp. SP isolate with application time before planting showed the best results compared to other treatments. Keywords: Application Time, Fusarium sp., Streptomyces sp.  INTISARI                                                 Penyakit layu fusarium pada tanaman cabai merah disebabkan oleh jamur patogen Fusarium sp. Penyakit ini tergolong ke dalam penyakit penting tanaman cabai merah karena dapat menyebabkan kerusakan hingga 50%. Salah satu Upaya dalam mengatasi penyakit layu fusarium adalah menggunakan agensia hayati Streptomyces sp. yang berasal dari lahan bawang merah dan tomat di Pare, Kediri. Tujuan dari penelitian ini adalah untuk mengetahui mengetahui pengaruh apikasi Streptomyces sp. terhadap penyakit layu fusarium pada tanaman cabai merah dengan berbagai macam waktu aplikasi. Penelitian ini menggunakan Rancangan Acak Lengkap Faktorial dengan perlakuan jenis isolate SP dan BMP, serta perlakuan waktu aplikasi sebelum tanam, bersamaan tanam dan setelah tanam. Masing-masing perlakuan diulang sebanyak 5 kali dan diberikan 5 unit tanaman pada masing-masing ulangan. Parameter pengamatan dilakukan terhadap masa inkubasi, keparahan penyakit, tinggi tanaman, jumlah daun, umur berbunga dan jumlah bunga. Aplikasi Streptomyces sp. isolat SP dengan waktu aplikasi sebelum tanam menunjukkan hasil terbaik dibandingkan dengan perlakuan yang lain. Kata kunci: Waktu Aplikasi, Fusarium sp., Streptomyces sp. 
UJI TINGKAT KERAPATAN SPORA DAN WAKTU APLIKASI Beauveria bassiana TERHADAP MORTALITAS ULAT GRAYAK (Spodoptera frugiperda J.E. Smith) PADA TANAMAN JAGUNG Frimadani, Latifah; Widajati, Wiludjeng; Mujoko, Tri
Agrika Vol. 18 No. 2 (2024)
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v18i2.6396

Abstract

ABSTRAKInsektisida kimia sintetis masih banyak digunakan kalangan petani untuk mengendalikan hama ulat grayak (Spodoptera frugiperda J.E Smith) yang menyerang tanaman jagung. Penggunaan insektisida kimia secara berlebihan menimbulkan pengaruh negatif bagi lingkungan dan kesehatan. Alternatif pengendalian S. frugiperda yang ramah lingkungan perlu dilakukan dengan memanfaatkan jamur entomopatogen, salah satunya berasal dari spesies Beauveria bassiana. Penelitian ini dilakukan untuk mengetahui tingkat kerapatan spora B. bassiana dan waktu aplikasi yang efektif terhadap mortalitas S. frugiperda pada tanaman jagung. Penelitian disusun secara rancangan acak lengkap (RAL) faktorial. Faktor pertama berupa kerapatan spora (106, 107, 108, dan 109 spora/ml), akuades steril dan insektisida kimia sintetis deltametrin sebagai kontrol. Faktor kedua adalah waktu aplikasi jamur (pagi dan sore hari). Hasil penelitian menunjukkan aplikasi B. bassiana pada waktu sore hari dengan kerapatan 108 dan 109 spora/ml mengakibatkan mortalitas larva S. frugiperda sebesar 60% dan 73.3%. Waktu aplikasi B. bassiana pada sore hari mengakibatkan persentase mortalitas lebih tinggi dibanding pada pagi hari. ABSTRACTSynthetic chemical insecticides are still widely used by farmers to control armyworm pests (Spodoptera frugiperda J.E Smith) which attack corn plants. Excessive use of chemical insecticides has negative effects on the environment and health. Environmentally friendly alternative control of S. frugiperda needs to be done by utilizing entomopathogenic fungi, one of which comes from the Beauveria bassiana species. This research was conducted to determine the density level of B. bassiana spores and the effective application time for S. frugiperda mortality on corn plants. The research was structured in a factorial Completely Randomized Design (CRD). The first factor was spore density (106, 107, 108, and 109 spores/ml), sterile distilled water and the synthetic chemical insecticide deltamethrin as a control. The second factor was the time of fungal application (morning and evening). The results show that application of B. bassiana in the afternoon with a density of 108 and 109 spores/ml resulted in S. frugiperda larvae mortality of 60% and 73.3%. The application time of B. bassiana in the afternoon resulted in a higher mortality percentage than in the morning.
Study of Streptomyces spp. to control purple blotch disease caused by Alternaria porri in shallot plant Risdiyanti, Rateh Lailatul; Rahmadhini, Noni; Suryaminarsih, Penta; Mujoko, Tri
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.43647

Abstract

Alternaria porri is a fungal pathogen that causes purple blotch on shallots, this fungus can cause crop yield loss of 3 – 57%. A. porri was obtained from the Sumber Brantas onion farm and then isolated by planting infected tissue. Streptomyces spp. is a filamentous bacterium that is abundant in the soil and can be used as a biological agent, decomposer and plant growth promote. Streptomyces spp. was obtained one isolate from shallot land location in Pare, Kediri, East Java (BMP: Bawang Merah Pare) and three isolates from Sidera, Palu, Central Sulawesi (BMS: Bawang Merah Sidera) (BMS1, BMS2, BMS3). The purpose of this study was to determine the efficacy of Streptomyces spp. to control A porri consist of in vitro and In vivo antagonist test. Antagonist test was carried out by dual culture method and the Streptomyces spp. which can inhibit the development of A porri would be used antagonist test   in vivo. The study was designed using a Factorial Complete Randomized Design with two factors, candidate isolates of biological control agents and concentrations of Streptomyces spp. be diluted into 5%, 10%, and 15%, it will applied on shallot plant Bauji variety. The results of the In vitro test shown the highest inhibition zone from BMP 17.75% while BMS1 13.75%, BMS2 8,75%, and 8.50%. in vivo test shown lowest disease severity value in BMP 15% concentration was 1.13% while diseases severity of BMS1 was 1.27%, and BMS2 was 1.80%. Therefore, Streptomyces spp. has potency as an alternative pesticide for sustainable agriculture.
Insect Diversity Study on Shallot Plants: Comparison of Ecological Engineering and Conventional Cultivation Systems Seftiarini, Amelia; Mujoko, Tri; Windriyanti, Wiwin
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 4 (2025): August 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i4.1476-1488

Abstract

This study examines the cultivation of shallots using an ecological engineering approach to enhance insect diversity and natural pest control. The applied methods include compost application, refugia planting, and the use of biological agents to reduce synthetic pesticide dependence. The study compares two management systems: ecological engineering farmland (EF) and conventional farmland (CF), with insect data collected using various trapping methods. The results show that EF had a higher insect population (23,428 individuals) compared to CF (14,880 individuals). A total of 181 morphospecies from 10 orders, 85 families, and 170 genera were identified, with Coleoptera being the dominant order and predatory insects prevailing in both farmlands. The diversity index was higher in EF (3.079) than in CF (2.725). The evenness index was also higher in EF (0.608–0.624) than in CF (0.561–0.603), indicating a more stable ecosystem. The dominance index was low in both farmlands (0.003–0.188), showing no single species significantly dominated. The community similarity index was relatively high at 0.666 (vegetative), 0.651 (generative), and 0.712 (one growing season). Although the t-test showed no significant differences, EF tends supports more sustainable shallot farming by enhancing biodiversity and ecosystem stability. Further research is needed to evaluate long-term impacts and the implementation of polyculture systems to strengthen agricultural ecosystems.
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Co-Authors Ali, Haidar Alvia Cindy Apriliani Arga Dwi Indrawan Ariani Trisna Murti Ariani Trisna Murti Arika Purnawati Arika Purnawati Bakti Wisnu Widjajani Dewi ANGGRAENI Dita Megasari Eben, Philipus Wahyu Baskoro Edyson Indawan Ekayanti, Savira Aprilia Endang Triwahyu Prasetyawati Ersyaf, Ihza Muhammad Fira Mawardani Firmansyah, Moch Ryan Frimadani, Latifah Haryani, Wiwik Sri Hasni Wardahni Herry Nirwanto Hidayati Karamina HIDAYATI, KIKI Ika Nur Fitriana Indah Pemuda Jati Laksono, Roy Laras Setyowati Lius, Michelle Lukman Hakim M Rifqi Haikal Makhziah Moch Nur Yudha Moch. Arifin Noni Rahmadhini Nur Syiami, Aning Penta SURYAMINARSIH Penta SURYAMINARSIH Penta Suryaminarsih Penta Suryaminarsih Purnomo Edi Sasongko Ramadhani Mahendra Kusuma Ratri, Tesha Lutfika Risdiyanti, Rateh Lailatul Rulinggar, Nia Seftiarini, Amelia Sri Wiyatiningsih Sukartiningrum Sumargono, ' Torino Benarivo Widajati, Wiludjeng Wiludjeng Widajati Wiludjeng Widayati Windriyanti, Wiwin Yenny WURYANDARI Yuwono, F. Tsara Nafisah Zakaria Wildan Fachrezzy