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All Journal Vegetalika Jurnal Pengendalian Hayati AGRIKA Cropsaver : Journal of Plant Protection MAPETA Agridevina : Berkala Ilmiah Agribisnis Plumula : Berkala Ilmiah Agroteknologi Kultivasi BIOEDUSCIENCE Jurnal Pertanian Agros Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) JURNAL AGROHITA Jurnal Ilmiah Hijau Cendekia PEDULI: Jurnal Imiah Pengabdian Pada Masyarakat Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal Pembelajaran dan Biologi Nukleus Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Agrotropika Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi International Journal on Food, Agriculture and Natural Resources (IJFANRES) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Tri MUJOKO
Program Studi Agroteknologi, Universitas Pembangunan Nasional ”Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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EFEKTIVITAS Streptomyces sp. SEBAGAI AGEN BIOREMEDIASI CEKAMAN PESTISIDA DI PERTANAMAN BAWANG MERAH (Allium ascalonicum L.) KECAMATAN BAGOR KABUPATEN NGANJUK Lius, Michelle; Mujoko, Tri; Nirwanto, Herry
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.6847

Abstract

ABSTRAK Pestisida merupakan bahan beracun yang berpotensi menimbulkan dampak negatif terhadap lingkungan hidup dan keanekaragaman hayati. Pengaplikasian pestisida secara langsung pada tanaman dapat meninggalkan residu yang berdampak pada kesehatan serta mengendap di dalam tanah dan menyebabkan kesuburan tanah terganggu. Bioremediasi merupakan pengembangan di bidang bioteknologi lingkungan yang memanfaatkan agen biologis untuk mengendalikan pencemaran.  Streptomyces sp. dapat menjadi agen bioremediasi yang membantu menurunkan nilai residu klorpirifos. Penelitian ini bertujuan untuk mengetahui kemampuan Streptomyces sp. sebagai agen bioremediasi serta untuk mengetahui konsentrasi Streptomyces sp. yang efektif. Penelitian dilakukan dengan menggunakan rancangan acak lengkap (RAL) dengan perlakuan kontrol atau tanpa Streptomyces sp., penambahan konsentrasi Streptomyces sp. 10 ml/100 ml aquades dan 20 ml/100 ml aquades. Hasil penelitian menunjukkan bahwa perlakuan konsentrasi Streptomyces sp. pada tanah tercemar residu klorpirfos dengan konsentrasi 20 ml mampu menurunkan nilai residu klorpirifos sebanyak 94% yaitu dari 1.08 ppm menjadi 0.06 ppm dalam waktu 21 hari.    
UJI EFEKTIVITAS EKSTRAK BAWANG PUTIH TERHADAP PERTUMBUHAN JAMUR PATOGEN Colletotrichum sp. SECARA IN VITRO Firmansyah, Moch Ryan; Purnawati, Arika; Mujoko, Tri
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.7270

Abstract

Peningkatan permintaan cabai di Indonesia diiringi dengan tantangan produksi, salah satunya adalah penyakit antraknosa yang disebabkan oleh Colletotrichum sp. Penyakit ini menurunkan hasil dan kualitas buah, dan umumnya dikendalikan dengan fungisida kimia. Penggunaan fungisida kimia menimbulkan risiko terhadap kesehatan dan lingkungan, sehingga diperlukan alternatif pengendalian hayati. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak bawang putih sebagai agen hayati terhadap Colletotrichum sp. secara in vitro dan membandingkannya dengan fungisida propineb. Penelitian menggunakan rancangan acak lengkap faktorial (RALF) dengan dua faktor, yaitu jenis perlakuan (ekstrak bawang putih dan fungisida kimia) dan konsentrasi (5%, 10%, dan 15%). Parameter yang diamati meliputi diameter koloni, persentase hambatan, intensitas serangan pada buah, serta karakteristik makro dan mikroskopis jamur. Hasil menunjukkan bahwa ekstrak bawang putih 15% mampu menghambat pertumbuhan jamur sebesar 38.73% dan intensitas serangan sebesar 21.33%, sedangkan fungisida propineb 5% menunjukkan hambatan tertinggi sebesar 78.43% dan intensitas serangan terendah sebesar 2.67%. Hasil ini menunjukkan bahwa ekstrak bawang putih memiliki potensi antijamur. namun efektivitasnya masih lebih rendah dibandingkan propineb.
POTENSI BIOPESTISIDA FOBIO DALAM MENEKAN PENYAKIT BERCAK DAUN (Helminthosporium oryzae) PADA TANAMAN PADI GOGO ORGANIK DI MOJOKERTO Wiyatiningsih, Sri; Eben, Philipus Wahyu Baskoro; Mujoko, Tri
Agrika Vol. 19 No. 2 (2025): NOVEMBER 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i2.7358

Abstract

Penyakit bercak daun yang disebabkan oleh Helminthosporium oryzae merupakan salah satu penyakit yang sering menyerang tanaman padi. Terdapat strategi pengendalian dalam pertanian berkelanjutan yang dapat diterapkan, salah satunya menggunakan biopestisida ramah lingkungan yang dapat menekan perkembangan penyakit tanaman. Penggunaan biopestisida Fobio diharapkan mampu mengandung berbagai mikroorganisme, baik yang sengaja diisolasi dari perakaran tanaman maupun yang terkandung didalam medium pembawanya. Tujuan penelitan ini adalah mengetahui potensi berbagai konsentrasi biopestisida Fobio dalam menekan perkembangan patogen  H. oryzae penyebab penyakit bercak daun pada tanaman padi gogo organik. Penelitian ini menggunakan rancangan acak kelompok (RAK) dengan 4 perlakuan dan 3 ulangan. Perlakuan dalam penelitan ini adalah KT sebagai kontrol (konsentrasi 0 ml/liter), FA (konsentrasi 5 ml/liter), FB (konsentrasi 10 ml/liter), FC (konsentrasi 15 ml/liter) dengan dosis 8 liter/plot. Hasil penelitian menunjukkan bahwa formula biopestisida Fobio pada konsentrasi 10 ml/liter paling efektif dalam menghambat infeksi patogen penyakit bercak coklat yang disebabkan oleh H. oryzae dibanding perlakuan lainnya. Pada perlakuan ini intensitas serangan penyakit akibat H. oryzae adalah yang paling rendah, yaitu 53.47% pada 10 MST, menghasilkan bobot basah gabah 979.0 gram dan bobot kering gabah 887.33 gram.
Biodegradation Potential of Streptomyces sp. Against propineb-Based 70% Fungicide Under In Vitro Conditions Sari, Nadya Naurah; Mujoko, Tri; Wiyatiningsih, Sri
CROPSAVER - Journal of Plant Protection Vol 8, No 2 (2025)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v8i2.66871

Abstract

The intensive use of propineb-based fungicides in agriculture can negatively affect the environment due to toxic and persistent residues in soil. An environmentally friendly approach to reduce these impacts is bioremediation using microorganisms such as Streptomyces sp. This study aimed to examine the tolerance and biodegradation potential of Streptomyces sp. toward 70% propineb fungicide under in vitro conditions. The research was conducted at the Plant Health 1 Laboratory, Universitas Pembangunan Nasional “Veteran” East Java, from June to August 2025. The experiments were carried out in two stages: (1) a tolerance assay on Glucose Nutrient Agar containing propineb at 0; 0,50; 1,00; 1,50; and 2,00 g/L, and (2) a degradation assay using a dual-culture method with Colletotrichum sp. as a bioindicator. Observation variables included Total Plate Count (TPC) of Streptomyces sp., colony diameter of Colletotrichum sp., and the relative inhibition level (THR). Results showed that Streptomyces sp. was able to grow at all fungicide concentrations. The highest population was found in P0 (0 g/L) with 1,42×1013 CFU/mL with growth decreasing as propineb concentration increased, reaching the lowest level of 3,22×10¹⁰ CFU/mL in P4 (2.00 g/L). In the degradation assay, Colletotrichum sp. colony diameter decreased from 3,38 cm in P0 (0 g/L)  to 2,24 cm in P4 (2,00 g/L). Meanwhile, THR values increased consistently with fungicide concentration, rising from 3,17% in P0 (0 g/L)  to 35,48% in P4 (2,00 g/L).
The Potency of Streptomyces spp. from Shallot Land as Entomopathogen of Onion Caterpillar Pest Spodoptera exigua Pratista, Ariq Dies; Windriyanti, Wiwin; Suryaminarsih, Penta; Mujoko, Tri; Indrawan, Arga Dwi; Harsiyanti, Harsiyanti
International Journal on Food, Agriculture and Natural Resources Vol 6, No 4 (2025): IJ-FANRES
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v6i4.569

Abstract

Spodoptera exigua is a major pest of shallots (Allium ascalonicum L.), capable of causing up to 100% yield loss if uncontrolled. Streptomyces sp. is a soil-dwelling Actinomycetes bacterium that can be used to control S. exigua. This study aimed to determine the potential of Streptomyces spp. isolates from shallot fields as an entomopathogen against S. exigua. The research employed a Factorial Complete Randomized Design (CRD). The first factor consisted of one Streptomyces spp. isolate obtained from the Pare-Kediri region (BMP) and three isolates from Sidera-Palu (BMS1, BMS2, and BMS3). The second factor was the concentration of the Streptomyces sp. suspension consist of 0%; 5%; 10%; and 15% with three replication per treatment (48 units total). Data on larval mortality and damage intensity were analyzed using RStudio via ANOVA and DMRT (α=5%). The BMP (15%) treatment demonstrated the highest efficacy, achieving 76.67% larval mortality while maintaining the lowest damage intensity of 0.20% on shallot plants. These results highlight BMP isolate at 15% concentration as the most promising biocontrol agent and support its further field evaluation as a sustainable alternative to chemical insecticides.
KAJIAN POLA SEBARAN PENYAKIT BULAI DENGAN ANALISIS CITRA DRONE Haikal, M Rifqi; Nirwanto, Herry; Mujoko, Tri
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 2 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i2.6105

Abstract

Jagung merupakan tanaman pangan yang penting di Indonesia dengan menjadi sumber pangan pokok ketiga setelah padi dan gandum. Penurunan produksi jagung dapat diakibatkan oleh Peronosclerospora spp. penyebab penyakit bulai yang merupakan penyakit utama yang meyerang tanaman jagung di Indonesia. Upaya pengendalian penyakit bulai harus diikuti dengan melakukan survei atau pemantauan di lahan pertanaman untuk melihat gejala kerusakan dan pola sebaran penyakit tersebut. Pemantauan di lapangan secara langsung terdapat banyak kekurangan seperti hasil yang bersifat subjektif dan membutuhkan waktu dan tenaga yang lebih banyak. Penelitian ini bertujuan untuk mendapatkan informasi mengenai penggunaan drone untuk mendeteksi gejala dan pola sebaran penyakit bulai sehingga lebih efektif dan efisien. Penelitian ini dilaksanakan di lahan tanaman jagung di Desa Sumbertebu, Kecamatan Bangsal, Kabupaten Mojokerto. Pengamatan dilakukan selama enam minggu dengan dua metode yakni pengamatan secara langsung dan melalui hasil analisis citra drone. Hasil penelitian menunjukkan bahwa pola sebaran penyakit bulai adalah acak. Citra drone dapat mendeteksi gejala serta menunjukkan pola sebaran penyakit bulai dengan memerhatikan ketinggian, intensitas cahaya matahari, dan objek di sekitar tanaman jagung.
Early Detection of Pathogenic Fungi Curvularia sp. on Rice Seeds (Oryza sativa) Based on Modified Infrared Image Analysis Anggraini, Novita; Nirwanto, Herry; Mujoko, Tri
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/17568

Abstract

Background: Curvularia sp. is a seed-borne pathogenic fungus that can reduce rice plant productivity. At the same time, conventional seed health testing methods still rely on visual observation and require a relatively long incubation time. This study aims to evaluate the potential of infrared image analysis based on a modified imaging system for early detection of Curvularia sp. infection in rice seeds. Methods: Seed health testing was performed using the blotter test method. At the same time, image acquisition was performed with a digital microscope equipped with an infrared light source, and images were analyzed using pseudocoloring and RGB-based color segmentation. Results: The results showed differences in infrared signal intensity patterns in Curvularia sp.-inoculated seeds, which could be identified on the fourth day after inoculation, earlier than visual observation, which showed symptoms on the fifth day. Detection accuracy was calculated using a confusion matrix based on visual observation as the reference method, with a sample size of 50 seeds per observation day, yielding an average detection accuracy of 91% over seven days of observation. Conclusions: The modified infrared image analysis method has the potential to serve as an early detection method for Curvularia sp. infection in rice seeds, although its performance depends on the limitations of the imaging system and the validation method used.
The Effect of Packaging and Encapsulation Temperature of Red Chili Seeds with Trichoderma sp. on Viability and Inhibition of Fusarium sp. Ratu, Dewanggie Sasmita; Nirwanto, Hery; Mujoko, Tri
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18020

Abstract

Background: Fusarium sp. is one of the main threats to chili cultivation, thereby reducing its economic value. Therefore, the use of biological microorganisms, such as Trichoderma sp., is an environmentally friendly alternative for controlling Fusarium sp. Application techniques for Trichoderma sp. biological agents include seed coating or encapsulation. However, ensuring the quality and effectiveness of encapsulated products during storage and distribution remains a concern. Thus, this study aims to examine the effects of packaging type and storage temperature on the viability and efficacy of Trichoderma sp. biological agents encapsulated in red chili seeds (Capsicum annuum L.) for inhibiting Fusarium sp. Methods: This study applied a completely randomized design (CRD) factorial with two factors, namely packaging material (aluminum foil and plastic) and storage temperature (5°C, 28°C, and 36°C), resulting in 7 treatments with three replications plus a control, resulting in a total of 21 experimental units. Results: Aluminum foil packaging stored at 28°C showed the highest viability of Trichoderma sp. and the most effective antagonistic activity against Fusarium sp. Conclusion: These results provide a sustainable, environmentally friendly solution for biological-agent-based seed storage.
Uji Konsentrasi Metabolit Sekunder Bakteri Bacillus sp. terhadap Jamur Colletotrichum sp. Pada Benih Cabai Rawit Metode Blotter Test Nurul Afida; Arika Purnawati; Tri Mujoko
Vegetalika Vol 14, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.108950

Abstract

Low-quality seeds can negatively affect the success of agricultural practices by reducing yield and increasing susceptibility to pathogen infection. Pathogens may infect seeds starting from the field, during transportation, and throughout storage. The utilization of Bacillus sp. as a biological control agent has been extensively studied due to its ability to produce secondary metabolites with antifungal properties. This study aimed to determine the effective concentration level of secondary metabolites of Bacillus sp. Bth-22 in suppressing Colletotrichum sp. infection on chili pepper (Capsicum frutescens L.) seeds using the blotter test method. The experiment was arranged in a completely randomized design (CRD) with a single factor consisting of concentration levels (10%, 15%, 20%, 25%, and 30%), sterile distilled water, and the chemical fungicide propineb as controls. The results showed that treatment with various concentrations of secondary metabolites of Bacillus sp. Bth-22 significantly affected the infection level, with concentrations of 30% (G) at 36%; 25% (F) and KP (B) at 38.6%; 20% (E) at 40%; 15% (D) at 42.6%; and 10% (C) at 44%, compared with the negative control. In addition, treatments also suppressed infection levels, with 30% (G) at 43.3%; 25% (F) and KP (B) at 39.3%; 20% (E) at 37%; 15% (D) at 33.6%; and 10% (C) at 31%. Furthermore, application of Bacillus sp. Bth-22 secondary metabolites induced abnormalities in fungal hyphae, such as curling, twisting, and bending, indicating antifungal activity of the bioactive compounds produced by Bacillus sp. Bth-22.
The Effect of Shelf Life and the Effectiveness of Biopesticide Fobio in Controlling Stem Rot Disease in Vanilla Plants (Vanilla planifolia Andrews) Mutiara Alyzza Bilqis; Sri Wiyatiningsih; Tri Mujoko
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol. 8 No. 1 (2026): Jurnal Agronomi Tanaman Tropika (JUATIKA) Vol. 8 No. 1 January 2026
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v8i1.5017

Abstract

Stem rot disease caused by Fusarium oxysporum poses a significant challenge to vanilla (Vanilla planifoliaAndrews) cultivation. The use of microbial-based biopesticides, such as FOBIO, provides an environmentallyfriendly alternative to reduce reliance on chemical fungicides. This study aimed to evaluate the effects of FOBIObiopesticide storage age and concentration on disease intensity and vanilla plant growth (Vanilla planifoliaAndrews). The research was conducted from March to June 2025 in Dusun Tukum using a factorial completelyrandomized design with two factors: FOBIO storage age (6 months and 1 year) and concentrations (5, 7.5, and 10mL/L of water), with three replications totaling 105 plants. Data were analyzed using Analysis of Covariance(ANCOVA), followed by the Least Significant Difference (LSD) test at a 5% significance level. The resultsdemonstrated that the FOBIO application significantly reduced disease intensity compared to the control (p <0.05). The most effective treatment was FOBIO stored for 1 year at a concentration of 7.5 mL/L of water (FP23B),resulting in a final disease intensity of 6.365 compared to 38.255 in the control. FOBIO also increased leaf numberand stimulated plant growth during the vegetative phase, including accelerating flower development. The FOBIObiopesticide is an environmentally friendly alternative for controlling stem rot in vanilla and serves as a biologicalcontrol agent with potential to support sustainable agriculture.
Co-Authors Ali, Haidar Alvia Cindy Apriliani Arga Dwi Indrawan Ariani Trisna Murti Arika Purnawati Bakti Wisnu Widjajani Dewi ANGGRAENI Dita Megasari Eben, Philipus Wahyu Baskoro Edyson Indawan Ekayanti, Savira Aprilia Endang Triwahyu Prasetyawati Ersyaf, Ihza Muhammad Fira Mawardani Firmansyah, Moch Ryan Frimadani, Latifah Haikal, M Rifqi Harsiyanti, Harsiyanti Haryani, Wiwik Sri Hasni Wardahni Herry Nirwanto Hery Nirwanto, Hery Hidayati Karamina HIDAYATI, KIKI Ika Nur Fitriana Indah Pemuda Indrawan, Arga Dwi Jati Laksono, Roy Laras Setyowati Lius, Michelle Lukman Hakim M Rifqi Haikal Makhziah Moch Nur Yudha Moch. Arifin Mutiara Alyzza Bilqis Nabila Naylofar Noni Rahmadhini Novita Anggraini Nur Syiami, Aning Nurul Afida Penta Suryaminarsih Penta Suryaminarsih Penta SURYAMINARSIH Penta SURYAMINARSIH Pratista, Ariq Dies Purnomo Edi Sasongko Ramadhani Mahendra Kusuma Ratri, Tesha Lutfika Ratu, Dewanggie Sasmita Risdiyanti, Rateh Lailatul Rulinggar, Nia Salma Alfiani Nadiva Sari, Nadya Naurah Sri Wiyatiningsih Sukartiningrum Sumargono, ' Torino Benarivo Vikayanti Vikayanti Widajati, Wiludjeng Wiludjeng Widajati Wiludjeng Widayati Windriyanti, Wiwin Yenny WURYANDARI Yuwono, F. Tsara Nafisah Zakaria Wildan Fachrezzy