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Validation of UV-Visible Spectrophotometry for Measuring Rhodamine B Content in Crackers Silva Oktriana; Suci Rizki Nurul Aeni; Ira Prima Sari
Journal of Applied Food and Nutrition Vol 2, No 1 (2021)
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.656 KB) | DOI: 10.17509/jafn.v2i1.41829

Abstract

Rhodamine B is a synthetic dye that is often misused as a food coloring in crackers. Rhodamine B consumption through food can irritate the digestive tract, impair liver function, and cancer. The purpose of the study was to determine the content of rhodamine B in crackers. This study used a quantitative method, and purposive sampling was employed as a sampling technique. Method validation was carried out against a UV-Visible spectrophotometer in testing Rhodamine B in crackers. Validation of the method produces a linear regression equation of y = 0.2901x + 0.0082, with a correlation coefficient value (r2) = 0.9985, and a Vxo value of 2.65%. The instrument validation shows a Limit of Detection (LOD) of 0.1582 ppm and a Limit of Quantification (LOQ) of 0.5274 ppm. The results from the accuracy-test showed an average %diff of 0.88%; 3.79%, and -6.88%, while the average percentages of recovery were 101%, 104, and 93% respectively. Precision testing results showed that the value of % RSD 2/3 CV Horwitz. Qualitative measurements showed that all samples tested for Rhodamine B in this study showed negative results. This study suggested that UV-Visible spectrophotometry is a valid method that can be used to measure levels of Rhodamine B in food.
PEMANFAATAN AMPAS DAUN TEH PADA PROSES BIOSORPSI LOGAM BERAT Cr(VI) PADA AIR SUNGAI CITARUM Suci Rizki Nurul Aeni; Farhan Baehaki; Syifa Zayna Muwahiddah
Jurnal Penelitian Saintek Vol 27, No 2 (2022)
Publisher : Directorate of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v2i27.52841

Abstract

Limbah Cr(VI) dapat mengganggu kesehatan karena bersifat toksik, maka perlu dilakukan pengolahan air sungai terlebih dahulu. Salah satu Teknik pengolahan alternatif yaitu menggunakan metode biosorpsi dengan biosorben ampas daun teh. Namun hasil penyerapan itu tergantung oleh beberapa faktor salah satunya yaitu waktu kontak. Tujuan penelitian ini untuk mengetahui waktu kontak optimum terhadap proses biosorpsi Cr(VI) dengan menggunakan biosorben ampas daun teh. Penelitian ini menggunakan metode deskriptif kuantitatif. Sampel yang digunakan air Sungai Citarum yang diambil dari empat titik yaitu di Desa Pangauban, Desa Cilampeni, Desa Nanjung dan Desa Lagadar. Pengukuran kadar dilakukan menggunakan Spektrofotometer UV-Vis pada panjang gelombang 540 nm. Berdasarkan penelitian ini diperoleh waktu kontak optimum yaitu 45 menit dengan presentase penurunan kadar dari masing-masing sampel yaitu 73,25% (Desa Pangauban), 75,94% (Desa Cilampeni), 71,88%, (Desa Nanjung) dan 74,31% (Desa Lagadar). Oleh karena itu, waktu kontak merupakan faktor yang perlu diperhatikan dalam proses biosorpsi untuk pengolahan limbah logam berat Cr(VI). Pemanfaatan ampas daun teh pada proses biosorpsi logam berat Cr(VI) pada air sungai CitarumCr(VI) waste can interfere with health because it is toxic, so it is necessary to treat river water first. One alternative treatment is using the biosorption method with tea leaf dregs biosorbent. However, the absorption results depend on several factors, one of which is contact time. The purpose of this study was to determine the optimum contact time for the biosorption process of Cr(VI) using tea leaf dregs biosorbent. This study used a quantitative descriptive method. The samples used were Citarum River water taken from four points, namely in Pangauban Village, Cilampeni Village, Nanjung Village and Lagadar Village. The concentration measurement was carried out using a UV-Vis Spectrophotometer at a wavelength of 540 nm. Based on this study, the optimum contact time was 45 minutes with the percentage reduction in levels of each sample, namely 73.25% (Pangauban Village), 75.94% (Cilapeni Village), 71.88%, (Nanjung Village) and 74, 31% (Lagadar Village). Therefore, contact time is a factor that needs to be considered in the biosorption process for the treatment of Cr(VI) heavy metal waste
THE EFFECT OF ZEOLITE ADMINISTRATION IN THE CONSORTIUM OF HEAVY METAL REDUCING BACTERIA Pb ON LIQUID WASTE OF TEXTILE INDUSTRY Ni'matul Murtafi'ah; Suci Rizki Nurul Aeni; Erika Setyowati
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 1 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i1.36353

Abstract

Textile industry wastewater treatment must be managed properly so as not to cause environmental pollution that has an impact on human health. Industrial wastewater treatment by utilizing microbial activity with the addition of zeolite with varying concentrations measured using UV-Vis Spectrophotometry. This study aims to find out the effect of variations in zeolite concentration on bacterial consortiums in reducing lead heavy metals (Pb) and find out the characteristics of bacteria reducing heavy metals Pb after added zeolite. The results of the study using UV-Vis Spectrophotometry showed that the concentration of zeolite 3% was able to reduce heavy metals Pb on the 14th day with consecutive levels of 0.473 with an initial lead content of 2,460 ppm. The most significant decrease occurred in the addition of a zeolite concentration of 3%. This is shown in its effectiveness value of 86.772%. From this study, it can be concluded that the administration of a concentration of 3% in a consortium of bacteria can lower the levels of heavy metals Pb. Based on the characteristics of bacteria in biochemical tests, namely the confectionery test (Maltose, Glucose, Sucrose) obtained positive results, negative Lactose test, red /red TSIA test, negative marker test, and positive. Basil-shaped gram-negative bacteria refer to the genus Desulfobacter.
Identifikasi Bakteri Pereduksi Logam Pb dalam Bioremediasi Sampel Air Sungai Citarum Menggunakan Analisis Gen 16s rRNA: Identification of Pb Metal Reducting Bacteria in Bioremediation from Citarum River Water Samples Using 16s rRNA Gene Analysis Ni'matul Murtafi'ah; Suci Rizki Nurul Aeni
Borneo Journal of Medical Laboratory Technology Vol. 5 No. 2 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v5i2.5102

Abstract

The bioremediation technique is an effort to remove the heavy metal Pb in samples of Citarum river water. High concentrations of heavy metals harm the environment, so Pb metal-reducing bacteria are needed as a pollution solution. Bioremediation uses bacteria. Bacteria are identified by molecular methods, namely sequencing analysis techniques, because they are more accurate. To determine the nucleotide sequence of Pb metal-reducing bacteria isolates and which bacterial species can reduce Pb metal. The methodology used is primary data collection, namely conducting research directly, namely the stages of bacterial purification through gram staining techniques, pure colonies, then proceeding to the DNA isolation stage. The isolated DNA is then electrophoresed. The electrophoresis results are then purified and proceed to the sequencing stage. The feasibility test results were to obtain pure DNA from the reducing bacteria. The next step was PCR amplification of the 16S rRNA gene of Pb-reducing bacteria. The research results started at the DNA isolation stage and obtained a purity of 1.80. The PCR results were in the form of amplification of the 16S rRNA gene of Pb-reducing bacteria. Electrophoresis obtained the results of DNA bands measuring ± 1400 bp—phylogenetic tree analysis with 99.60% relatedness to Bacillus cereus, which can reduce Pb. Bacillus cereus species can carry out detoxification mechanisms against the toxic effects of Pb metals, such as—biosorption, bioaccumulation, reduction, solubilization, precipitation (formation of extracellular complexes), and methylation.
“Padi Lingko” to Reduce Hexavalent Chromium Levels (Cr(VI)) in Citarum River Water Sample Aeni, Suci Rizki Nurul; Nurdin, Nurdin; Ansori, Ikhsan Faisal
EduChemia: Jurnal Kimia dan Pendidikan Vol 8, No 2 (2023)
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/educhemia.v8i2.21545

Abstract

Clean water is increasingly difficult to obtain, even though water is a chemical compound that is very important for living creatures' lives. Industrial and domestic community waste are a big problem in polluting the Citarum River, causing the water to become unfit. One of the toxic heavy metals found in Citarum River water is chromium (Cr(VI)). The technique that can be used to remove the heavy metal Cr(VI) in Citarum river water samples is adsorption using biological material (biosorption). Biosorption is the process of absorbing metal ions by materials originating from nature. In this research, jengkol peel was used. A modification of this research from previous research is the design of the "Padi Lingko" tool. This research aims to determine the results of the function test of the Padi Lingko tool and measure chromium(VI) levels in treated Citarum river water samples. The results of the analysis show that the power supply measurement results are in accordance with the expected values. The speed test results show appropriate values, namely at a low speed of 213 rpm, a medium speed of 1109 rpm, and a high speed of 1225 rpm. The timer test results show the appropriate time, with an average error factor of -0.02%. The results of the storage bucket test showed that when operated, the bucket did not produce vibrations or noise and could hold water. Apart from that, there are no leaks in the collection bucket. The test results show that the drive motor works well and can rotate the shaft without stuttering. The Padi Lingko tool could be used and succeeded in reducing Cr(VI) until a final level of 0.0529 mg/L was obtained.
PERUBAHAN KANDUNGAN ASIP MELALUI PROSES PENYIMPANAN DAN PENCAIRAN Diani Aliansy; Mira Miraturrofi’ah; Suci Rizki Nurul Aeni
Jurnal Kesehatan - STIKes Prima Nusantara Vol 12 No 2 (2021): JURNAL KESEHATAN
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v12i2.400

Abstract

Latar Belakang  : Air Susu Ibu (ASI) adalah nutrisi ideal bagi bayi karena mengandung banyak zat perlindungan terhadap berbagai penyakit. Rekomendasi World Health Organization (WHO), sebaiknya bayi diberikan ASI selama paling sedikit 6 bulan. Namun bagi ibu bekerja, ASI Eksklusif terkadang sulit untuk dipenuhi. Di Indonesia jumlah wanita bekerja Tahun 2016 sebesar 50,77% meningkat di tahun 2017 menjadi 50,89%, dan > 50% diantaranya adalah ibu menyusui dan memiliki anak di bawah 2 tahun. [1] Masa cuti melahirkan secara tidak langsung ikut berkonrtibusi dalam tidak terpenuhinya ASI Eksklusif. [2] Banyak ibu bekerja yang mensiasati dengan melakukan penyimpanan ASI. Oleh karenanya, pengetahuan dan sikap ibu dalam manajemen ASIP (air susu perah) sangat penting. Hal tersebut diharapkan tidak merubah kandungan gizi saat ASIP diberikan kepada bayi.Tujuan : Mengetahui kandungan makronutrien, jumlah mikroorganisme dan tingkat keasaman ASIP pada berbagai suhu dan lama penyimpanan serta cara pencairannya.Metode : Jenis penelitian yang digunakan adalah studi eksperimen. ASI di perah kemudian dihomogenkan lalu ditampung pada botol kaca yang telah disterilkan. Analisis protein menggunakan Bradford, karbohidrat dengan metode fenol sulfat, lemak menggunakan metode Liebermann-Burchard. pH menggunakan indikator universal dan jumlah mikroorganisme menggunakan plate count.Hasil : karbohidrat ASIP optimal selama 8 hari di suhu 150C dan dicairkan terlebih dahulu di refrigerator.  Protein ASIP masih dapat disimpan selama 6 bulan pada suhu -20 0C. lemak ASIP optimal selama 5 hari pada suhu 4 0C. Semakin lama penyimpanan kadar pH semakin menurun. Jemaah mikroorganisme dapat di tekan di suhu -20 0C.Simpulan Manajemen penyimpanan ASIP optimal di suhu < 15 C. Metode pencairan terbaik adalah melalui refrigerator.
PEMANFAATAN BIOSORBEN BIJI BUAH RUDRAKSHA (Elaeocarpus Ganitrus) DALAM MENURUNKAN KADAR LOGAM BERAT CR(Vi) PADA SAMPEL AIR SUNGAI CITARUM Istiqomah, Mahmudatul; Aeni, Suci Rizki Nurul; Wahid, Aziz Ansori
Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Vol. 7 No. 1 (2025): Publikasi Penelitian Kimia dan Terapannya 2025
Publisher : Program Studi Kimia, Fakultas MIPA, Universitas PGRI Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/jc.v7i1.5010

Abstract

Abstract Hexavalent chromium Cr(VI) is a harmful heavy metal that frequently discovered in rivers that contaminated by industrial textile waste. Biosorption is a technique that utilizes natural materials to absorb metal ions from water. This research aims to asess efficacy of biosorbent derived from rudraksha ruit seeds in diminishing Cr(VI) concentration in water samples from the Citarum River through biosorption mechanism. The biosorption was conducted by applying the Citarum River water samples with the biosorbent at varying masses of 0.1 g, 0.5 g, 1.0 g, 1.5 g, and 2.0 g. Based on the research findings, the biosorption process reduced the concentrations of Cr(VI) in the water samples from the Citarum River. The highest reduction in Cr(VI) levels occurred at a biosorbent mass of 2.0 g, with percentage reductions of 92.02% and 96.24% for water samples from Rancamanyar Village and Andir Subdistrict, respectively. Therefore, it can be inferred that the biosorbent from rudraksha fruit seeds is effective in lowering Cr(VI) levels in Citarum River water samples. Keywords: Heavy metal, Cr(VI), Biosorption, Rudraksha fruit seeds, Biosorbent Abstrak Kromium heksavalen Cr(VI) adalah logam berat berbahaya yang sering ditemukan di sungai yang terkontaminasi oleh limbah tekstil industri. Biosorpsi adalah teknik yang memanfaatkan bahan alami untuk menyerap ion logam dari air. Penelitian ini bertujuan untuk menilai efektivitas biosorben yang berasal dari biji buah rudraksha dalam mengurangi konsentrasi Cr(VI) dalam sampel air dari Sungai Citarum melalui mekanisme biosorpsi. Biosorpsi dilakukan dengan menerapkan sampel air Sungai Citarum dengan biosorben pada massa yang bervariasi yaitu 0,1 g, 0,5 g, 1,0 g, 1,5 g, dan 2,0 g. Berdasarkan temuan penelitian, proses biosorpsi mengurangi konsentrasi Cr(VI) dalam sampel air dari Sungai Citarum. Penurunan tertinggi kadar Cr(VI) terjadi pada massa biosorben 2,0 g, dengan penurunan persentase sebesar 92,02% dan 96,24% untuk sampel air dari Desa Rancamanyar dan Kecamatan Andir, masing-masing. Sehingga, dapat disimpulkan bahwa biosorben dari biji buah rudraksha efektif dalam mengurangi kadar Cr(VI) pada sampel air Sungai Citarum. Keywords: Logam berat, Cr(VI), Biosorpsi, Biji buah rudraksha, Biosorben
Pemanfaatan Maggot Black Soldier Fly dalam Pengolahan Sampah Organik di Wilayah Kampung Manglayang, Desa Cihanjuang RT001/RW003 Yuningsih, Rima; Murtafi’ah, Ni’matul; Aeni, Suci Rizki Nurul
Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Vol 9, No 4 (2025): Jurnal Pelayanan dan Pengabdian Masyarakat (PAMAS)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM Universitas Respati Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/pamas.v9i4.5980

Abstract

Waste is still a big problem in various parts of Indonesia, including in the West Java area. According to the West Java Provincial Environment Agency (2024), waste from all districts and cities in the West Java area reaches 25,000 tons per month. Organic waste management in Manglayang Village only relies on cleaners who will transport garbage every week, during a week the garbage is allowed to accumulate in front of the yard so that it causes an unpleasant odor and a lot of flies. The purpose of this study is to reduce organic waste by the bioconversion method which uses larvae to transform organic waste. This research uses the Participaory Action Research technique, namely by approaching socialization and discussion with the community. The results of the study showed the effectiveness of maggot in degrading organic waste for 21 days as much as 18 kilograms with 56 grams of ungranulated waste. The use of BSF maggot in managing organic waste in Manglayang Village 003/001 can be chosen as an alternative to reduce waste which is a problem.