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All Journal Biotropika Journal of Pharmacopolium Acta Biochimica Indonesiana Nusantara Science and Technology Proceedings Current Research on Biosciences and Biotechnology Jurnal Sains dan Teknologi Farmasi Indonesia

Nur Asni Setiani

Sekolah Tinggi Farmasi Indonesia

Author-ID : 3254149

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Engineering Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Articles

STANDARDISASI MIKROBIOLOGI KEFIR DARI SUSU KAMBING DAN SUSU SAPI Putri, Yola Desnera; Setiani, Nur Asni; Ayuningtyas, Yudyana; Ledianasari, Ledianasari
Journal of Pharmacopolium Vol 3, No 2 (2020)
Publisher : P3M STIKes Bakti Tunas Husada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36465/jop.v3i2.623

Pengaruh Desinfektan dan Lama Perendaman pada Sterilisasi Eksplan Daun Sukun (Artocarpus altilis (Parkinson ex. F.A Zorn) Fosberg) Nur Asni Setiani; Fitri Nurwinda; Dewi Astriany
Biotropika: Journal of Tropical Biology Vol 6, No 3 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2018.006.03.01

Daun sukun (Artocarpus altilis) merupakan herbal yang banyak dimanfaatkan untuk pengobatan. Kultur jaringan menjadi alternatif dalam meningkatkan produktivitas daun sukun dan metabolit sekunder yang dihasilkannya. Tahap pertama yang menentukan keberhasilan perbanyakan tanaman melalui kultur jaringan adalah teknik sterilisasi. Penelitian ini bertujuan mendapatkan metode sterilisasi terbaik dengan melihat pengaruh penggunaan natrium hipoklorit 5,25% dan alkohol 70% serta waktu perendaman terhadap penurunan kontaminasi. Pada penelitian ini terdapat 10 kelompok yang terdiri dari kelompok kontrol positif, kontrol negatif, perendaman dengan alkohol 70% selama 5 menit dan 10 menit, perendaman dengan natrium hipoklorit 5,25% selama 5 menit dan 10 menit, serta kombinasi natrium hipoklorit 5,25% dan alkohol 70% selama 5 menit dan 10 menit. Parameter pengamatan meliputi waktu pertama kontaminasi, persentase kontaminasi jamur dan bakteri, dan penampilan eksplan yang diamati selama 40 hari. Hasil penelitian pada penggunaan masing-masing desinfektan, baik alkohol 70% maupun natrium hipoklorit 5,25% dengan waktu perendaman 10 menit menunjukkan tidak adanya kontaminasi jamur dan bakteri, tetapi pada perlakuan natrium hipoklorit 5,25% terjadi perubahan warna eksplan menjadi kecoklatan. Kombinasi dari kedua desinfektan menunjukkan adanya kontaminasi jamur dan bakteri serta adanya perubahan warna eksplan. Sterilisasi eksplan daun sukun yang terbaik dapat dilakukan dengan cara perendaman menggunakan alkohol 70% selama 10 menit.

Studies on biosurfactant produced using Exiguobacterium profundum Nur Asni Setiani; Welly Octaviyani; Syarif Hamdani; Irma Mardiah
Acta Biochimica Indonesiana Vol. 2 No. 2 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i2.37

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties. Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum. Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing. Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper. Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.

The effect of temperature, incubation and storage time on lactic acid content, pH and viscosity of goat milk kefir Yola Desnera Putri; Nur Asni Setiani; Sohadi Warya
Current Research on Bioscences and Biotechnology Vol. 2 No. 1 (2020)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2020.2.1/HPMQ5042

Kefir is a fermented beverage that has probiotic properties and often used as a cosmetic or ointment raw material. Its lactic acid content is classified as AHA (alpha hydroxy acid) which is known good for skin health. The objectives were to study the influence of temperature, fermentation and storage time on the amount of lactic acid, pH and viscosity of kefir. Fermentation of kefir was performed at two different temperatures (room temperature and 37oC) for 24 and 48 h. Storage condition of kefir products was performed at cold and room temperature for 4 to 28 d. The content of lactic acid was based on the total organic acid determined using acid-base titration. The results showed that the average content of lactic acid in 48 h-fermented kefir at room temperature and 37oC were 0.9 to 2.2% with pH and viscosity characteristics were 4.1 to 4.3 and 1400 to1600 cPs, respectively. Meanwhile, during 24 d of storage, the average content of lactic acid was 1.97 to 3.54%, where pH and viscosity characteristics were 3.5 to 4.5 and 3400 to 6400 cPs, respectively. The optimum storage time of goat milk kefir is obtained on days 4 to 12 and they can be stored for up to 24 days without deterioration of kefir products.

Utilization of Waste Frying Oil as A Source of Carbon in The Production of Biosurfactant using Exiguobacterium profundum Irma Mardiah; Kartika Puspitaningrum; Syarif Hamdani; Nur Asni Setiani
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2104

Biosurfactant is a secondary metabolite produced by microorganisms that can be used as an alternative to environmentally friendly surfactants. Exiguobacterium profundum is one of the biosurfactants producers that potentially to be used in the pharmaceutical field. The use of waste frying oil as a carbon source can be used as a solution in overcoming the high cost of producing biosurfactants. The purpose of this study was to obtain optimum conditions in the production of biosurfactants by utilizing waste frying oil as a carbon source. In this study, variations in the optimized production conditions included the concentration of waste frying oil, labeled 2%, 3%, 4%, and 5%, and the medium pH at 6, 7, and 8. The study was using Mineral Salt Medium as production medium, the amount of inoculum concentration was 10% v/v, agitation speed 160 rpm, and incubation at room temperature. The optimum conditions for biosurfactant production were determined based on the best emulsification index. The biosurfactant extraction was carried out using a combination of chloroform and methanol (2:1) solvents. The best concentrations of waste frying oil for Exiguobacterium profundum was 5%, and the best medium pH was 7. Biosurfactants produced from Exiguobacterium profundum amounted to 8,2 g/L with an emulsification index 63,2%.

PERBANDINGAN PRODUKTIVITAS ISOLASI IMMUNOGLOBULIN YOLK (IgY) DARI TELUR AYAM NEGERI, TELUR AYAM KAMPUNG, DAN TELUR BEBEK DENGAN METODE PEG-PRECIPITATION Nur Asni Setiani
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 5, No 1 (2016)
Publisher : Sekolah Tinggi Farmasi Indonesia

AbstrakTelur ayam dan unggas lainnya merupakan sumber protein hewani yang umum digunakan sebagai bahan pangan. Selain itu, telur memiliki potensi penting sebagai sumber antibodi sehingga dapat digunakan sebagai pabrik biologis alternatif untuk produksi antibodi konvensional. Imunoglobulin yolk (IgY) merupakan imunoglobulin unggas dengan fungsi biologi seperti IgG pada mamalia. Eksperimen ini bertujuan untuk membandingkan produktivitas isolasi IgY dari telur ayam negeri, telur ayam kampung, dan telur bebek dengan menggunakan metode presipitasi PEG sehingga dapat memaksimalkan produksi antibodi. Pengukuran spektrofotometri pada panjang gelombang (λ) 280 nm dilakukan untuk menentukan kuantitas IgY. Ekstraksi dari telur ayam negeri, telur ayam kampung, dan telur bebek masing-masing menghasilkan IgY sebesar 7,896mg/mL, 10,069mg/mL, dan 26,308mg/mL. Hasil SDS-PAGE yang dikuantifikasi dengan menggunakan ImageJ menunjukkan telur bebek memiliki produktivitas isolasi Imunoglobulin Y (IgY) yang paling tinggi (0,242 mg/g) dibandingkan telur ayam kampung (0,103 mg/g) dan telur ayam negeri (0,037 mg/g). Produktivitas imunoglobulin dipengaruhi kandungan nutrisi pada kuning telur. Kata kunci : Immunoglobulin Y, telur ayam negeri, telur ayam kampung, telur bebek, PEG AbstractChicken and other poultry eggs are a source of animal protein commonly used as food. In addition, eggs have an important potential as a source of antibodies that can be used as an alternative biological factory for the production of conventional antibodies. Yolk immunoglobulin (IgY) is a poultry immunoglobulin which has a biological function such as mammalian IgG. This experiment aims to compare the productivity of IgY isolation from chicken eggs, free-range eggs, and duck eggs using the PEG precipitation method so that it can be maximized for antibody production. The quantity of IgY is determined by the measurement of the spectrophotometer (λ = 280 nm). The results showed the extraction of chicken eggs, free-range eggs, and duck eggs each yielded IgY of 7,896mg / mL, 10,069mg / mL, and 26,308mg / mL. Results of SDS-PAGE quantified using ImageJ showed duck eggs have the highest productivity of Immunoglobulin Y (IgY) isolation (0.242 mg / g) compared to free-range eggs (0.103 mg / g) and chicken eggs (0.037 mg / g). Immunoglobulin productivity is influenced by nutrient content in egg yolks. Keywords : Immunoglobulin Y, chicken eggs, free-range eggs, duck eggs, PEG

PENGARUH PEMBERIAN KEFIR DENGAN PENAMBAHAN EKSTRAK BUAH STROBERI (Fragaria X Ananassa Duchesne) DAN DAGING BUAH ASAM JAWA(Tamarindus indica L) TERHADAP PENURUNAN KADAR KOLESTEROLTIKUS WISTAR JANTAN Maria - Ulfah; Nur Asni Setiani; Tria - Yuganingsih
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 7, No 2 (2018)
Publisher : Sekolah Tinggi Farmasi Indonesia

AbstrakHiperkolesterolemia adalah suatu keadaan ketika kadar kolesterol dalam darah meningkat dan terjadi akumulasi trigliserida, kolesterol, dan asam lemak di dalam sel hati. Salah satu cara pencegahan yaitu dengan mengonsumsi kefir, minuman fermentasi yang bermanfaat memperbaiki saluran pencernaan dan mampu menurunkan tingginya kadar lipid dalam darah. Kefir dapat dikombinasikan dengan buah atau sayuran untuk memperkaya rasa ataupun meningkatkan manfaatnya. Buah stroberi dan buah asam jawa merupakan tanaman obat di Indonesia yang memiliki sifat antihiperkolestrolemia. Penelitian ini bertujuan mengetahui pengaruh pemberian kefir, kefir ekstrak buah stroberi dan kefir ekstrak asam jawa terhadap penurunan kadar kolesterol terhadap tikus putih Wistar jantan yang diinduksi lemak tinggi. Pemberian kefir, kefir stroberi dan kefir asam jawa dilakukan selama 21 hari. Kadar kolesterol diperiksa dengan metode biosensor electrode-based menggunakan alat ukur easy touch®. Data dianalisis dengan metode ANOVA satu arah dan uji lanjutaan LSD. Hasil penelitian menunjukkan terjadinya penurunan kadar kolesterol darah dengan pemberian kefir, kefir stoberi, dan kefir asam jawa secara berurutan sebesar 14%, 25%, dan 21%. Penurunan terbaik kadar kolesterol darah tikus putih Wistar jantan yaitu dengan pemberian kefir stroberi.Kata Kunci: Kolesterol, Kefir, Stroberi, Asam Jawa, TikusAbstractHypercholesterolemia is a condition when cholesterol levels in the blood increase and there is accumulation of triglycerides, cholesterol, and fatty acids in liver cells. One method of prevention is by consuming kefir, a fermented beverage that is useful in improving the digestive tract and is able to reduce high levels of lipids in the blood. Kefir can be combined with fruit or vegetables to enrich the taste or increase its benefits. Strawberry and tamarind are medicinal plants in Indonesia which have antihypercholesterolemia properties. This study aims to determine the effect of kefir, strawberry kefir and tamarind kefir on the reduction of cholesterol levels in male Wistar white rats which are high fat induced. An experimental animals were given kefir, strawberry kefir and tamarind kefir for 21 days. Cholesterol levels were examined by electrode-based biosensor method using an easy touch® measuring instrument. Data were analyzed by one-way ANOVA and LSD. The results showed a decrease in cholesterol levels in the blood by giving kefir, strawberry kefir, and tamarind kefir sequentially by 14%, 25%, and 21%. The best decrease in blood cholesterol levels of male Wistar white rats is strawberry kefir.Kata Kunci: Cholesterol, Kefir, Strawberry, Tamarind, Rats

PEMANFAATAN AIR KELAPA DAN LIMBAH KECAP SEBAGAI SUBSTRAT DALAM PRODUKSI PIGMEN KAROTENOID Seno Aulia Ardiansyah; Nur Asni Setiani; Anggi - Restiasari; Landiyani - Putri; Eka - Noviana
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 8, No 1 (2019)
Publisher : Sekolah Tinggi Farmasi Indonesia

Abstrak Antioksidan dapat ditemukan di alam atau dibuat secara sintetik. Salah satu antioksidan alami adalah karotenoid. Neurospohora sitophila biasa disebut sebagai jamur yang berasal dari oncom banyak mengandung karotenoid. Limbah atau ampas kecap merupakan limbah yang masih banyak mengandung protein yang tinggi. Air kelapa dapat digunakan sebagai substrat dalam fermentasi karotenoid. Penelitian ini bertujuan memanfaatkan air kelapa dan limbah kecap sebagai substart serta menentukan pengaruh penambahan kofaktor logam dalam produksi pigmen karotenoid. Neurospora sitophila diisolasi dari oncom merah, diinokulasi pada media PDA (Potato Dextrose Agar) dan diinkubasi selama 5 hari pada suhu ruangan. Suspensi spora dihasilkan dari proses inkubasi Neurospora sitophila. Limbah kecap dan air kelapa sebagai substrat ditambahkan dengan konsentrasi 10%, 15%, 20% dan 25% v/v. Selain itu ditambahkan ion logam Mg2+sebagai trace element untuk meningkatkan aktivitas enzim dan meningkatkan produksi karotenoid dalam media PDA. Dilakukan analisis pigmen karotenoid dengan menggunakan Spektrofotometer UV-Vis pada panjang gelombang 450 nm dengan standar beta karoten.. Hasil penelitian menunjukkan penambahan ion logam Mg dengan substrat limbah kecap menghasilkan karotenoid sebesar 6,485 gram sedangkan penambahan ion logam Mg dengan substrat air kelapa dapat menghasilkan karotenoid sebesar 10,022 gram. Kata kunci : Neurospora sitophila, air kelapa, limbah kecap, substrat, ion logam Mg2+ , Spektrofotometer Uv-Vis Abstract Antioxidants can be found in nature or made synthetically. One of nature antioxidants is carotenoids. Neurospora sitophila also known as oncom molds that contains carotenoids. Dregs of soy sauce is soy-based waste and still has a high protein content. Coconut water can be used as a substrate in carotenoid fermentation. Neurospora sitophila isolated from red oncom to increase amount of production in PDA (Potato Dextrose Agar) media and incubated for 5 days at room temperature. Spore suspension created from results incubation of Neurospora sitophila. Make dregs of soy sauce substrate and coconut water as substrates with concentration on 10%, 15%, 20% and 25% v/v. Conducted substrate dregs of soy sauce and coconut water testing with metal ions addition Mg2+ as trace element to improve enzyme activity and increase amount of carotenoid production on PDA media. The result of carotenoid is checked for absorbance using UV-Vis Spectrophotometer at 450 nm and compared with standard beta-carotene. The results showed that metal ions Mg2+ addition on substrate dregs of soy sauce can increase carotenoids production 6,485 grams and metal ions Mg2+ 6 addition on substrate coconut water can increase carotenoids production 10,022 grams. Keywords : Neurospora sitophila, coconut water, dregs of soy-sauce, substrates, metal ions, UV-Vis Spectrophotometer

Co-Authors Anggi - Restiasari Ayuningtyas, Yudyana Dewi Astriany Eka - Noviana Fitri Nurwinda Hamdani, Syarif Irma Mardiah Kartika Puspitaningrum Landiyani - Putri Ledianasari, Ledianasari Maria Ulfah Seno Aulia Ardiansyah Sohadi Warya Tria - Yuganingsih Welly Octaviyani Yola Desnera Putri

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