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Peningkatan Kelarutan dan Disolusi Ibuprofen melalui Pembentukan Mikropartikel Metode Emulsification-Ionic-Gelation menggunakan Polivinil Alkohol (PVA) sebagai Polimer Tripolifosfat (TPP) sebagai Agen Crosslink Ferdiansyah, Rival; Putri, Yola Desnera; Hamdani, Syarif; Julianto, Angga
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 3 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Ibuprofen dikenal sebagai obat antiinflamasi nonsteroid yang digunakan untuk analgetik dan antipiretik dan termasuk obat dengan kelarutan rendah, tetapi memiliki permeabilitas yang tinggi. Penelitian ini bertujuan untuk menghasilkan ibuprofen dengan kelarutan dan disolusi yang lebih tinggi dengan membentuk mikropartikel menggunakan metode emulsification-ionic gelation. Partikel ibuprofen dikarakterisasi menggunakan Scanning Elektron Microscopy dan Fourier Transform Infrared Spectroscopy (FTIR). Kemudian dilakukan uji kelarutan dan uji disolusi ibuprofen. Pengujian karakteristik menggunakan SE, mikropartikel pH 2, pH4, pH 6 mengalami penurunan ukuran partikel berturut-turut sebesar 97,48%, 84%, dan 72% dari ukuran ibuprofen. Kelarutan ibuprofen setelah proses emulsification-ionic-gelation dalam air pada pH 6 meningkat delapan kali, sedangkan disolusi ibuprofen tertinggi ditunjukkan oleh partikel ibuprofen pada formula 3 yang dapat mendisolusi ibuprofen lebih dari 44% dalam 30 menit pada media dapar HCl 0,1N dibandingkan dengan formula yang lain. Kesimpulan yang diperoleh adalah partikel ibuprofen setelah proses emulsification-ionicgelation dapat memperkecil ukuran partikel dan meningkatkan kelarutan tetapi tidak dengan disolusinya.Kata kunci: Ibuprofen, emulsification-ionic-gelation, polivinil alkohol, kelarutan, disolusi

Peningkatan Kelarutan dan Disolusi Ibuprofen melalui Pembentukan Mikropartikel Metode Emulsification-Ionic-Gelation menggunakan Polivinil Alkohol (PVA) sebagai Polimer Tripolifosfat (TPP) sebagai Agen Crosslink Rival Ferdiansyah; Yola Desnera Putri; Syarif Hamdani; Angga Julianto
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 3 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Ibuprofen dikenal sebagai obat antiinflamasi nonsteroid yang digunakan untuk analgetik dan antipiretik dan termasuk obat dengan kelarutan rendah, tetapi memiliki permeabilitas yang tinggi. Penelitian ini bertujuan untuk menghasilkan ibuprofen dengan kelarutan dan disolusi yang lebih tinggi dengan membentuk mikropartikel menggunakan metode emulsification-ionic gelation. Partikel ibuprofen dikarakterisasi menggunakan Scanning Elektron Microscopy dan Fourier Transform Infrared Spectroscopy (FTIR). Kemudian dilakukan uji kelarutan dan uji disolusi ibuprofen. Pengujian karakteristik menggunakan SE, mikropartikel pH 2, pH4, pH 6 mengalami penurunan ukuran partikel berturut-turut sebesar 97,48%, 84%, dan 72% dari ukuran ibuprofen. Kelarutan ibuprofen setelah proses emulsification-ionic-gelation dalam air pada pH 6 meningkat delapan kali, sedangkan disolusi ibuprofen tertinggi ditunjukkan oleh partikel ibuprofen pada formula 3 yang dapat mendisolusi ibuprofen lebih dari 44% dalam 30 menit pada media dapar HCl 0,1N dibandingkan dengan formula yang lain. Kesimpulan yang diperoleh adalah partikel ibuprofen setelah proses emulsification-ionicgelation dapat memperkecil ukuran partikel dan meningkatkan kelarutan tetapi tidak dengan disolusinya.Kata kunci: Ibuprofen, emulsification-ionic-gelation, polivinil alkohol, kelarutan, disolusi

Studies on biosurfactant produced using Exiguobacterium profundum Nur Asni Setiani; Welly Octaviyani; Syarif Hamdani; Irma Mardiah
Acta Biochimica Indonesiana Vol. 2 No. 2 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i2.37

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties. Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum. Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing. Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper. Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.

In Vitro Inhibition of α-Amylase and α-Glucosidase by Different Parts of Amla Plant (Phyllanthus emblica L) Novi I. Fauzi; Maria Ulfah; Syarif Hamdani; Saeful Hidayat
Pharmacology and Clinical Pharmacy Research Vol 3, No 3
Publisher : Universitas Padjadjaran, Indonesia

Amla plant (Phyllanthus emblica L) has been empirically used to treat diabetes. The aim of this study was to determine the most potential part of amla plant that can be used as α-glucosidase and α-amylase inhibitors. The fruit was subjected to cold extraction method, while the stem underwent soxhlet extraction process. Ethanol was used as solvent for both extracts. In-vitro inhibiton against α-glucosidase and α-amylase enzyme was measured by calculating IC50 of water fractions of stem and fruits. Fruit fraction exhibited the highest percentage of inhibitory activity on α-amylase (IC50=5.68% w/v), while the strongest inhibition against α-glucosidase was shown by leaves fraction (IC50=0.87% w/v). In conclusion, the stem of P. emblica L was potential inhibitor of α-amylase , while the leaves was potential α-glucosidase inhibitor.Keywords: Phyllanthus emblica, α-glucosidase inhibitor, α-amylase inhibitor, fruit, leaves

Utilization of Waste Frying Oil as A Source of Carbon in The Production of Biosurfactant using Exiguobacterium profundum Irma Mardiah; Kartika Puspitaningrum; Syarif Hamdani; Nur Asni Setiani
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2104

Biosurfactant is a secondary metabolite produced by microorganisms that can be used as an alternative to environmentally friendly surfactants. Exiguobacterium profundum is one of the biosurfactants producers that potentially to be used in the pharmaceutical field. The use of waste frying oil as a carbon source can be used as a solution in overcoming the high cost of producing biosurfactants. The purpose of this study was to obtain optimum conditions in the production of biosurfactants by utilizing waste frying oil as a carbon source. In this study, variations in the optimized production conditions included the concentration of waste frying oil, labeled 2%, 3%, 4%, and 5%, and the medium pH at 6, 7, and 8. The study was using Mineral Salt Medium as production medium, the amount of inoculum concentration was 10% v/v, agitation speed 160 rpm, and incubation at room temperature. The optimum conditions for biosurfactant production were determined based on the best emulsification index. The biosurfactant extraction was carried out using a combination of chloroform and methanol (2:1) solvents. The best concentrations of waste frying oil for Exiguobacterium profundum was 5%, and the best medium pH was 7. Biosurfactants produced from Exiguobacterium profundum amounted to 8,2 g/L with an emulsification index 63,2%.

TEKNIK STERILISASI EKSPLAN TUNAS KENTANG GRANOLA KEMBANG (Solanum Tuberosum L.) UNTUK KULTUR IN VITRO Syarif Hamdani; Delima Nugraha; Tiara Berliana; Umi Baroroh
Jurnal Kartika Kimia Vol 3 No 2 (2020): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v3i2.63

Potato (Solanum tuberosum L.) is a plant that widely used, both fresh and processed. However, the productivity of this plant is still low because the quality of seeds is still limited and is not good enough. The cultivar of granola kembang is potential to be developed because of the short harvest life and high yield. In vitro propagation through tissue culture can be done to produce high-quality seeds. The success of tissue culture is strongly influenced by explant sterilization techniques. Bacterial, fungal, and browning contamination can interfere with the process of culture propagation. In this study, six sterilization techniques were applied to potato shoot explants to get the right sterilization techniques for in vitro culture of potato. Various combinations of sterilant and their order of use are also used. Growth of stem length and number of leaves was also investigated for four weeks after planting (WAP). The results showed that sterilization using Tween-20, benomyl 50%, and agrimycin outside LAF and NaOCl 15% (v/v), NaOCl 10% (v/v), and alcohol 70% inside LAF could produce the highest percentage of sterile explant, 70%, compare to other techniques. Nevertheless, the sterilization technique used in this study did not affect the acceleration of stem growth and the number of leaves. Keywords: granola kembang, in vitro, potato, sterilization, tissue culture

PERBANDINGAN KADAR KATEKIN DARI BEBERAPA JENIS KUALITAS TEH HITAM (Camellia sinensis L.[O] Kuntze) DI PUSAT PENELITIAN TEH DAN KINA (PPTK) GAMBUNG Siti Uswatun Hasanah; Syarif - Hamdani; Adang - Firmansyah
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 1, No 1 (2012)
Publisher : Sekolah Tinggi Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58327/jstfi.v1i1.12

AbstrakKatekin merupakan salah satu senyawa metabolit dalam teh yang diketahui memiliki aktivitas farmakologis. Tujuan dari penelitian ini adalah menganalisis kadar katekin dari tiga kualitas teh hitam di Pusat Penelitian Teh dan Kina (PPTK) Gambung, Ciwidey. Ekstraksi katekin dimulai dengan maserasi menggunakan aseton, diikuti dengan fraksinasi menggunakan air-etil asetat. Analisis katekin dilakukan pada fraksi etil asetat menggunakan KLT dan spektrovotometri UV. Hasil menunjukkan bahwa semua the dalam penelitian ini memberi bercak pada KLT (Rf 0,9) dan memberikan λ maks serta bentuk spectrum yang sama. Hal ini menunjukkan bahwa semua teh hitam mengandung katekin dengan masing-masing besarnya kadar katekin teh kualitas 1 (BOP), the kualitas 8 (BP 2) dan teh kualitas 15 (BBL) secara berurutan adalah 18.290 ppm, 18.055 ppm dan 12.290 ppm. Disimpulkan bahwa semakin baik kualitas teh hitam maka semakin besar katekin yang terkandung. Kata kunci: Teh hitam, Katekin, KLT, Spektrofotometri UV. AbstractCatechin is one of metabolite compound in tea, which is known has pharmacological activity. This research aimed to analyze level of catechin in various kind of black tea that produced at Tea and Quinine Research Centre, Gambung, Ciwidey. Extraction of catechin begun by maseration by aceton, followed by fractination used water-ethyl acetate. Analisis of catechin has been done from ethyl acetate fraction used TLC and Spectrophotometer UV. The result showed that all types of tea gave spot on TLC (Rf 0,9) and gave same λ max as well as spectral form. It showed that all types of tea in this research had catechin with each level of catechin on top quality black tea (BOP), quality black tea 8 (BP 2) and quality black tea 15 (BBL) respectively were 18,290 ppm, 18,055 ppm and 12,290 ppm. It was conclude that better quality of black tea would has high level of catechin. Key words: Black tea, Catechin, TLC, Spectrophotometric UV.

SKRINING PEREAKSI SPOT TEST UNTUK DETEKSI KANDUNGAN FORMALIN PADA BAHAN PANGAN Windari - Syafitri; Adang - Firmansyah; Syarif - Hamdani
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 1, No 2 (2012)
Publisher : Sekolah Tinggi Farmasi Indonesia

AbstrakPenggunaan bahan tambahan makanan berbahaya seperti formalin akhir-akhir ini menjadi perhatian yang serius dikarenakan efeknya yang membahayakan kesehatan tubuh manusia dan berpotensi menyebabkan kanker. Penelitian bertujuan untuk melakukan skrining pereaksi yang dapat digunakan untuk mendeteksi formalin. Metode Spot Test digunakan untuk tujuan kepraktisan, sekaligus untuk mengetahui sensitivitas dan selektivitas pereaksi pada bahan pangan. Hasil pengujian beberapa pereaksi menunjukkan bahwa pereaksi Schiff merupakan pereaksi yang relatif paling memuaskan dengan selektivitas dan sensitivitas pereaksi hingga 10 ppm. Pengembangan pereaksi dengan menambahkan CuSO4 serta CuSO4 dan FeCl3 dapat meningkatkan sensitivitas sampai 0,01 ppm. Walaupun didapatkan sensitivitas yang memuaskan, akan tetapi selektivitas pereaksi ternyata masih kurang baik jika diuji terhadap formalin di dalam bahan pangan. Kata Kunci : Formalin, Spot test, Sensitivitas, Selektivitas. AbstractThe use of harmful food additives such as formalin lately become serious concern due to the effects that clinically reported to be toxic to humans body and potentially cause cancer. Research aimed to conduct screening of reagens that can be used to detect formalin. The Spot test methode is used for practical purposes and also to determine the sensitivity and selectivity some reagents in food. The test results showed that Schiff reagen was the most satisfactory reagent with its sensitivity at 10 ppm. Development of reagents by adding CuSO4 and CuSO4-FeCl3 can increase the sensitivity till 0,01 ppm. Although the sensitivity gave satisfaction results, but selectivity of the reagent was still not good if tested against formalin in food stuffs. Keywords : Formalin, Spot test, Sensitivity, Selectivity.

PENETAPAN KADAR LIKOPEN DARI BEBERAPA BUAH BERDAGING MERAH DENGAN METODE SPEKTROFOTOMETRI Deby - Tristiyanti; Syarif - Hamdani; Dian - Rohita
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 2, No 1 (2013)
Publisher : Sekolah Tinggi Farmasi Indonesia

AbstrakLikopen adalah senyawa karotenoid yang mempunyai khasiat sebagai antioksidan dan memberikan warna merah pada beberapa buah dan sayuran, diantaranya tomat, semangka dan jambu biji merah. Likopen diduga terdapat pada buah lain yang berwarna merah seperti arben. Penelitian ini dilakukan untuk menentukan kadar likopen dalam buah semangka, jambu biji merah dan arben dengan metode spektrofotometri visible. Analisis kualitatif dilakukan dengan membandingkan spektrum sampel dengan standar. Kadar likopen diukur pada panjang gelombang 472 nm dengan metode pengukuran adisi standar. Dari hasil penelitian ini diperoleh kadar likopen dalam buah semangka adalah 33 mg/100 g, jambu biji adalah 7,5 mg/100 g dan buah arben adalah 9 mg/100 g. Kata kunci: Likopen, Semangka, Jambu biji, Arben, Metode Spektrofotometri, Metode Adisi Standar. AbstractLycopene is a carotenoid that has antioxidant properties and imparts the red pigment in some fruits and vegetables, including in tomatoes, watermelon and red guava. Lycopene also allegedly contained in other red fruits such as arben. Study was performed with visible spectrophotometric method to determine lycopene in watermelon, red guava and arben. Spectrum of sample was compared with standar for qualitative analyzed. Measurement of lycopene level had been done with standard addition method at maximum wavelengths of 472 nm. 33 mg/100g lycopene contain in watermelon, 7,5 mg/100g lycopene contain in red guava and 9 mg/100g contain in arben was result from this study. Keywords: Lycopene, Watermelon, Guava, Arben, Spectrophotometric Method, Standard Addition Method.

BIODEGRADASI LIMBAH METANOL DENGAN MENGGUNAKAN RAGI Trichosporon sp. UICC-S41111 Syarif - Hamdani; Basuki - Hadi; Ferro - Subhan
JURNAL SAINS DAN TEKNOLOGI FARMASI INDONESIA Vol 2, No 1 (2013)
Publisher : Sekolah Tinggi Farmasi Indonesia

AbstrakLimbah metanol merupakan salah satu masalah pencemaran lingkungan hidup yang memerlukan penangan serius. Pada penelitian ini telah dilakukan biodegradasi limbah metanol dengan menggunakan ragi Trichosporon sp. UICC-S41111. Biodegradasi dilakukan dengan mencampurkan sejumlah limbah dengan suspensi biakan Trichosporon sp. UICC-S41111 yang disertai proses aerasi pada pH 6,0 dengan variasi penambahan pepton. Hasil penelitan menunjukkan bahwa Trichosporon sp. UICC-S41111 memiliki kemampuan untuk mendegradasi limbah metanol dengan penurunan nilai COD sebesar 86,8-88,7 % pada proses biodegradasi selama 4 hari. Dari hasil penelitian juga dapat diketahui bahwa penambahan pepton dapat meningkatkan kemampuan Trichosporon sp. UICC-S41111 dalam mendegradasi limbah metanol. Nilai COD dengan penambahan pepton (18.491,5 mg O2/L) menunjukkan penurunan yang cukup signifikan dibandingkan dengan biodegradasi tanpa penambahan pepton (21.543,5 mg O2/L). Kata Kunci: Biodegradasi, Limbah Metanol, Ragi Trichosporon sp. UICC-S41111 AbstractMethanol sewage is one of the environmental contamination problems that need serious handling. In this research, biodegradation of methanol sewage with Trichosporon sp. UICC-S41111 has been done. Biodegradation was done by mixing amount of sewage with Trichosporon sp. UICC-S41111 culture suspension with aeration process at pH 6 with peptone adding variation. The result of this research showed that Trichosporon sp. UICC-S41111 had ability to degrade methanol sewage with decreased COD value 86.8-88.7 % in the biodegradation process for 4 days. The result showed that peptone addition could increase the ability of Trichosporon sp. UICC-S41111 to degrade methanol sewage. COD values with the addition of peptone (18491.5 mg O2 / L) showed a significant decrease compared to biodegradation without the addition of peptone (21543.5 mg O2 / L). Keywords: Biodegradation, Methanol Sewage, Trichosporon sp. UICC-S41111

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