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Peningkatan Kelarutan dan Disolusi Ibuprofen melalui Pembentukan Mikropartikel Metode Emulsification-Ionic-Gelation menggunakan Polivinil Alkohol (PVA) sebagai Polimer Tripolifosfat (TPP) sebagai Agen Crosslink Ferdiansyah, Rival; Putri, Yola Desnera; Hamdani, Syarif; Julianto, Angga
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 3 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1663.164 KB) | DOI: 10.15416/ijpst.v4i3.13864

Abstract

Ibuprofen dikenal sebagai obat antiinflamasi nonsteroid yang digunakan untuk analgetik dan antipiretik dan termasuk obat dengan kelarutan rendah, tetapi memiliki permeabilitas yang tinggi. Penelitian ini bertujuan untuk menghasilkan ibuprofen dengan kelarutan dan disolusi yang lebih tinggi dengan membentuk mikropartikel menggunakan metode emulsification-ionic gelation. Partikel ibuprofen dikarakterisasi menggunakan Scanning Elektron Microscopy dan Fourier Transform Infrared Spectroscopy (FTIR). Kemudian dilakukan uji kelarutan dan uji disolusi ibuprofen. Pengujian karakteristik menggunakan SE, mikropartikel pH 2, pH4, pH 6 mengalami penurunan ukuran partikel berturut-turut sebesar 97,48%, 84%, dan 72% dari ukuran ibuprofen. Kelarutan ibuprofen setelah proses emulsification-ionic-gelation dalam air pada pH 6 meningkat delapan kali, sedangkan disolusi ibuprofen tertinggi ditunjukkan oleh partikel ibuprofen pada formula 3 yang dapat mendisolusi ibuprofen lebih dari 44% dalam 30 menit pada media dapar HCl 0,1N dibandingkan dengan formula yang lain. Kesimpulan yang diperoleh adalah partikel ibuprofen setelah proses emulsification-ionicgelation dapat memperkecil ukuran partikel dan meningkatkan kelarutan tetapi tidak dengan disolusinya.Kata kunci: Ibuprofen, emulsification-ionic-gelation, polivinil alkohol, kelarutan, disolusi
Peningkatan Kelarutan dan Disolusi Ibuprofen melalui Pembentukan Mikropartikel Metode Emulsification-Ionic-Gelation menggunakan Polivinil Alkohol (PVA) sebagai Polimer Tripolifosfat (TPP) sebagai Agen Crosslink Rival Ferdiansyah; Yola Desnera Putri; Syarif Hamdani; Angga Julianto
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 3 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1663.164 KB) | DOI: 10.15416/ijpst.v4i3.13864

Abstract

Ibuprofen dikenal sebagai obat antiinflamasi nonsteroid yang digunakan untuk analgetik dan antipiretik dan termasuk obat dengan kelarutan rendah, tetapi memiliki permeabilitas yang tinggi. Penelitian ini bertujuan untuk menghasilkan ibuprofen dengan kelarutan dan disolusi yang lebih tinggi dengan membentuk mikropartikel menggunakan metode emulsification-ionic gelation. Partikel ibuprofen dikarakterisasi menggunakan Scanning Elektron Microscopy dan Fourier Transform Infrared Spectroscopy (FTIR). Kemudian dilakukan uji kelarutan dan uji disolusi ibuprofen. Pengujian karakteristik menggunakan SE, mikropartikel pH 2, pH4, pH 6 mengalami penurunan ukuran partikel berturut-turut sebesar 97,48%, 84%, dan 72% dari ukuran ibuprofen. Kelarutan ibuprofen setelah proses emulsification-ionic-gelation dalam air pada pH 6 meningkat delapan kali, sedangkan disolusi ibuprofen tertinggi ditunjukkan oleh partikel ibuprofen pada formula 3 yang dapat mendisolusi ibuprofen lebih dari 44% dalam 30 menit pada media dapar HCl 0,1N dibandingkan dengan formula yang lain. Kesimpulan yang diperoleh adalah partikel ibuprofen setelah proses emulsification-ionicgelation dapat memperkecil ukuran partikel dan meningkatkan kelarutan tetapi tidak dengan disolusinya.Kata kunci: Ibuprofen, emulsification-ionic-gelation, polivinil alkohol, kelarutan, disolusi
Potensi Bacillus cereus dalam produksi biosurfaktan Nur Asni Setiani; Nia Agustina; Irma Mardiah; Syarif Hamdani; Dewi Astriany
Jurnal Biologi Udayana Vol 24 No 2 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2020.v24.i02.p09

Abstract

Biosurfactants from microorganisms have stable physical and chemical properties, environment friendly, biodegradable, renewable, cheaper and low toxicity that can be used in the pharmaceutical industry as antibacterial. The aim of this study was to examine the emulsification and characterization of biosurfactant from Bacillus cereus. Hemolysis and spreading oil tests was performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. The supernatant was measured for biosurfactant activity through an emulsification index and characterized using thin layer chromatography and antibacterial qualitative testing. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44,83%, 48,28%, 51,72%, 46,43%, and 51,50%. The formation of red stains on TLC plates with ninhydrin appearance showed lipopeptide groups. Antibacterial testing using Escherichia coli and Staphylococcus aureus showed the formation of clear zones around the disk paper. Bacillus cereus can produce biosurfactants of the lipopeptide group with the largest emulsification index of 51.72% and has antibacterial activity against both gram positive and gram negative.
Studies on biosurfactant produced using Exiguobacterium profundum Nur Asni Setiani; Welly Octaviyani; Syarif Hamdani; Irma Mardiah
Acta Biochimica Indonesiana Vol. 2 No. 2 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i2.37

Abstract

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties. Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum. Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing. Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper. Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.
In Vitro Inhibition of α-Amylase and α-Glucosidase by Different Parts of Amla Plant (Phyllanthus emblica L) Novi I. Fauzi; Maria Ulfah; Syarif Hamdani; Saeful Hidayat
Pharmacology and Clinical Pharmacy Research Vol 3, No 3
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.883 KB) | DOI: 10.15416/pcpr.v3i3.19980

Abstract

Amla plant (Phyllanthus emblica L) has been empirically used to treat diabetes. The aim of this study was to determine the most potential part of amla plant that can be used as α-glucosidase and α-amylase inhibitors. The fruit was subjected to cold extraction method, while the stem underwent soxhlet extraction process. Ethanol was used as solvent for both extracts. In-vitro inhibiton against α-glucosidase and α-amylase enzyme was measured by calculating IC50 of water fractions of stem and fruits. Fruit fraction exhibited the highest percentage of inhibitory activity on α-amylase (IC50=5.68% w/v), while the strongest inhibition against α-glucosidase was shown by leaves fraction (IC50=0.87% w/v). In conclusion, the stem of P. emblica L was potential inhibitor of α-amylase , while the leaves was potential α-glucosidase inhibitor.Keywords: Phyllanthus emblica, α-glucosidase inhibitor, α-amylase inhibitor, fruit, leaves
Utilization of Waste Frying Oil as A Source of Carbon in The Production of Biosurfactant using Exiguobacterium profundum Irma Mardiah; Kartika Puspitaningrum; Syarif Hamdani; Nur Asni Setiani
Nusantara Science and Technology Proceedings 2nd Bioinformatics and Biodiversity Conference
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2104

Abstract

Biosurfactant is a secondary metabolite produced by microorganisms that can be used as an alternative to environmentally friendly surfactants. Exiguobacterium profundum is one of the biosurfactants producers that potentially to be used in the pharmaceutical field. The use of waste frying oil as a carbon source can be used as a solution in overcoming the high cost of producing biosurfactants. The purpose of this study was to obtain optimum conditions in the production of biosurfactants by utilizing waste frying oil as a carbon source. In this study, variations in the optimized production conditions included the concentration of waste frying oil, labeled 2%, 3%, 4%, and 5%, and the medium pH at 6, 7, and 8. The study was using Mineral Salt Medium as production medium, the amount of inoculum concentration was 10% v/v, agitation speed 160 rpm, and incubation at room temperature. The optimum conditions for biosurfactant production were determined based on the best emulsification index. The biosurfactant extraction was carried out using a combination of chloroform and methanol (2:1) solvents. The best concentrations of waste frying oil for Exiguobacterium profundum was 5%, and the best medium pH was 7. Biosurfactants produced from Exiguobacterium profundum amounted to 8,2 g/L with an emulsification index 63,2%.
TEKNIK STERILISASI EKSPLAN TUNAS KENTANG GRANOLA KEMBANG (Solanum Tuberosum L.) UNTUK KULTUR IN VITRO Syarif Hamdani; Delima Nugraha; Tiara Berliana; Umi Baroroh
Jurnal Kartika Kimia Vol 3 No 2 (2020): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v3i2.63

Abstract

Potato (Solanum tuberosum L.) is a plant that widely used, both fresh and processed. However, the productivity of this plant is still low because the quality of seeds is still limited and is not good enough. The cultivar of granola kembang is potential to be developed because of the short harvest life and high yield. In vitro propagation through tissue culture can be done to produce high-quality seeds. The success of tissue culture is strongly influenced by explant sterilization techniques. Bacterial, fungal, and browning contamination can interfere with the process of culture propagation. In this study, six sterilization techniques were applied to potato shoot explants to get the right sterilization techniques for in vitro culture of potato. Various combinations of sterilant and their order of use are also used. Growth of stem length and number of leaves was also investigated for four weeks after planting (WAP). The results showed that sterilization using Tween-20, benomyl 50%, and agrimycin outside LAF and NaOCl 15% (v/v), NaOCl 10% (v/v), and alcohol 70% inside LAF could produce the highest percentage of sterile explant, 70%, compare to other techniques. Nevertheless, the sterilization technique used in this study did not affect the acceleration of stem growth and the number of leaves. Keywords: granola kembang, in vitro, potato, sterilization, tissue culture
Optimasi produksi biosurfaktan dari bakteri Bacillus cereus menggunakan minyak jelantah Irma Mardiah; Ika Fatimah; Nur Asni Setiani; Syarif Hamdani; Dewi Astriany
Jurnal Biologi Udayana Vol 26 No 2 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i02.p09

Abstract

Biosurfaktan merupakan zat penurun tegangan permukaan yang dihasilkan oleh mikroorganisme yang memiliki aktivitas antibakteri. Bacillus cereus merupakan salah satu bakteri penghasil biosurfaktan. Penelitian ini bertujuan untuk mendapatkan kondisi optimum produksi biosurfaktan dari Bacillus cereus dengan memanfaatkan minyak jelantah sebagai sumber karbon. Optimasi produksi biosurfaktan dilakukan pada variasi konsentrasi minyak jelantah 2%, 3%, 4%, 5% dan variasi pH media 6, 7, 8 serta dilakukan uji aktivitas menggunakan metode indeks emulsifikasi 24 jam (IE24). Penelitian ini menggunakan media produksi Mineral Salt Medium (MSM), konsentrasi inokulum sebanyak 10% (108 CFU/ml), inkubasi pada suhu ruang dengan kecepatan agitasi 160 rpm. Ekstraksi biosurfaktan menggunakan pelarut kloroform : metanol (2:1). Konsentrasi minyak jelantah terbaik adalah 3% dan nilai pH terbaik pada pH 6. Hasil produksi biosurfaktan dari bakteri Bacillus cereus adalah 8,9 g/L dengan nilai IE24 57,17%. Hasil dari penelitian ini diharapkan mendapatkan kondisi optimum untuk produksi biosurfaktan yang mengandung antimikroba yang dihasilkan oleh Bacillus cereus.
Integrasi Edukasi dan Skrining Talasemia: Model Pengabdian Masyarakat untuk Mahasiswa Kesehatan di Bandung Hamdani, Syarif; Simanjuntak, Nela; Rachmaniar, Revika
Jurnal Abdimas Kartika Wijayakusuma Vol 6 No 3 (2025): Jurnal Abdimas Kartika Wijayakusuma (In Progress)
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v6i3.635

Abstract

Talasemia merupakan penyakit genetik yang menyebabkan gangguan produksi hemoglobin dengan Kota Bandung tercatat sebagai salah satu wilayah dengan jumlah penyandang tertinggi di Indonesia. Pencegahan penyebaran talasemia dapat dilakukan melalui skrining pada individu pranikah, terutama mahasiswa. Program pengabdian masyarakat ini bertujuan untuk melakukan edukasi dan skrining talasemia pada mahasiswa kesehatan di Bandung sebagai upaya pencegahan talasemia pada generasi mendatang. Kegiatan ini diawali dengan seminar edukasi tentang talasemia, dilanjutkan dengan skrining melalui pemeriksaan darah dan pendampingan bagi peserta yang terindikasi sebagai pembawa sifat. Hasil menunjukkan peningkatan kesediaan peserta untuk mengikuti skrining setelah edukasi dari 90,81% menjadi 96,86%. Dari 432 peserta yang menjalani skrining, 7 orang terindikasi positif sebagai pembawa sifat talasemia. Pendampingan dilakukan dengan melibatkan tim Thalassemia Research Center, POPTI, dan dokter konsultan. Program pengabdian masyarakat ini menunjukkan bahwa edukasi berperan penting dalam meningkatkan kesadaran dan partisipasi dalam skrining talasemia. Kegiatan terintegrasi yang melibatkan edukasi, skrining, dan pendampingan pada generasi pranikah dapat menjadi langkah efektif dalam upaya memutus rantai penyebaran talasemia. Kolaborasi berbagai pihak dalam pelaksanaan program ini menunjukkan pentingnya pendekatan multidisipliner dalam upaya pencegahan talasemia di masyarakat.