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All Journal HAYATI Journal of Biosciences Science and Technology Indonesia
Harry Murti
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science Materials Science & Nanotechnology Physics

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Quantitative Analysis of hsa-miR-21-5p Expression in Human Umbilical Cord Mesenchymal Stem Cell-derived Exosomes Under Hypoxia Muhammad, Daisy Ramadhani; Barlian, Anggraini; Murti, Harry; Prawira, Matheus Alvin; Faza, Naufalia
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1426-1436

Abstract

Exosomes have emerged as a cell-free alternative to mesenchymal stem cells (MSCs). Hypoxic preconditioning might result in the production of exosomes with ideal properties. As such, the effect of hypoxia on hsa-mir-21-5p expression, which is known to contribute to the immunomodulatory activity of exosomes, should be investigated. This research aimed to enrich and characterize exosomes from human umbilical cord MSC (hUC-MSC) secretome samples secreted under hypoxia and normoxia, as well as to analyze the expression of hsa-miR-21-5p in each sample. Secretomes were collected from cultured cells under both hypoxic and normoxic conditions, and exosomes were subsequently enriched from the secretomes through ultrafiltration. In this research, it was found that particles in N-Exo and H-Exo exhibited round morphology, expressed exosomal CD81 and CD63 markers with an average size of 153.7 ± 31.3 nm and 137.7 ± 25.0 nm, respectively. N-Exo and H-Exo can also be internalized by mesenchymal stem cells (MSCs). qRT-PCR results then indicated that hsa-miR-21-5p expression was significantly lower in H-Exo (p < 0.05). In conclusion, hypoxic preconditioning, as performed in this research, was also found to affect hsa-miR-21-5p and downregulate its expression in hypoxic samples relative to controls.
Potential of Secretome Hydrogel for Wound Healing in LPS- and Scratch-Induced BJ Cells as an Inflammation Model Widowati, Wahyu; Rahmat, Deni; Faried, Ahmad; Nainggolan, Ita Margaretha; Priyandoko, Didik; Wargasetia, Teresa Liliana; Sugiaman, Vinna Kurniawati; Triharsiwi, Dwi Nur; Qlintang, Sandy; Murti, Harry; Azis, Rizal; Jeffrey, Jeffrey
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1242-1254

Abstract

Wound healing often requires specialized interventions to accelerate recovery due to prolonged inflammation and limited regenerative factors. Umbilical Cord Mesenchymal Stem Cells secretome (UCMSCs) comprises various cytokines and growth factors that can promote wound healing. This study aims to analyze the potential of a secretome-based hydrogel as a wound-healing agent using BJ fibroblast cells induced by lipopolysaccharide (LPS) and scratch injury as an inflammation model. The secretome hydrogel was formulated using Carbopol, Hydroxypropyl Methylcellulose (HPMC), Hydroxyethyl Cellulose (HEC), and secretome. Cytotoxicity was conducted using the WST-8 assay, while cell migration was evaluated through a scratch assay. Tumor Necrosis Factor-???? (TNF-????), Nuclear Factor kappa-B (NF-????B), and Interleukin-8 (IL-8) gene expression were analyzed via qRT-PCR. Additionally, malondialdehyde (MDA) levels were measured for oxidative stress assessment, whereas Connective Tissue Growth Factor (CTGF) and Transforming Growth Factor-????1 (TGF-????1) levels were quantified using ELISA and colorimetric assays. The secretome hydrogel exhibited no cytotoxic effects on BJ fibroblast cells and significantly enhanced cell migration. Moreover, it reduced the TNF-????, IL-8, and NF-????B expression, indicating anti-inflammatory activity. The hydrogel also decreased MDA levels while increasing TGF-β1 and CTGF expression, suggesting antioxidant properties and enhanced tissue regeneration in the inflammatory model. The secretome-based hydrogel presents a promising therapeutic approach for promoting chronic wound healing by modulating inflammation, reducing oxidative stress, enhancing tissue regeneration, and stimulating fibroblast migration.
Co-Authors Ahmad Faried Azis, Rizal Barlian, Anggraini Didik Priyandoko Faza, Naufalia Jeffrey ., Jeffrey Muhammad, Daisy Ramadhani Nainggolan, Ita Margaretha Prawira, Matheus Alvin Qlintang, Sandy RAHMAT, DENI Teresa Liliana Wargasetia Triharsiwi, Dwi Nur Vinna Kurniawati Sugiaman, Vinna Kurniawati WAHYU WIDOWATI