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All Journal HAYATI Journal of Biosciences Jurnal Biodjati Jurnal Ilmiah Tatengkorang
Barlian, Anggraini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Immunology & microbiology Public Health

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Characterization of Orexin Gene in Tilapia (Oreochromis niloticus):Regulator Feeding Appetite, and Correlation with Reproductive Factors Simanjuntak, Ricky Febrinaldy; Barlian, Anggraini; Wibowo, Indra; ., Rukisah
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.32063

Abstract

The mechanism of energy allocation related to increased appetite and feed intake in fish involves several neuropeptides encoded by specific genes. Orexin (OX) is a peptide secreted in the pars tuberalis of the hypophysis that acts as a stimulator in appetite increase (orexigenic factor). However, the expression of different orexigenic factors varies among vertebrate species, reflecting their unique types and lineages. Therefore, this study aimed to identify genes encoding appetite in Tilapia. RNA isolation, complementary Deoxyribose Nucleic Acid (cDNA) cloning, and DNA amplification were performed from brain samples of gonadally mature Tilapia. The PCR products were subsequently sent to Macrogen.Inc for sequencing. The amplification results of Orexin with β-Actin (positive control) observed using agarose gel electrophoresis showed that the size of the nucleotide base product of each gene was 196 bp and 197 bp. Confirmation of sequencing results carried out using the Basic Local Alignment Search Tool (BLAST) method - National Center for Biotechnology Information (NCBI) for Orexin and β-Actin were 97% and 100%. Based on these results, it can be concluded that each target gene isolated from the tilapia brain showed homology/similarity with the sequence available in the NCBI database
The Effect of Papaya Derived Exosome-like Nanoparticle as a Potential Photoprotective Agent in Nanocosmetics Farid, Faticha Nasharo Qisthia; Barlian, Anggraini; Wibowo, Indra
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.920-930

Abstract

Indonesia, as a tropical nation, experiences year-round ultraviolet radiation exposure. Excessive ultraviolet radiation, particularly UVB, accelerates premature skin aging, known as photoaging. UVB radiation induces photoaging by damaging DNA and increasing the production of reactive oxygen species (ROS), which leads to oxidative stress and results in increased apoptosis, melanogenesis, senescence, cell cycle arrest, and reduced cell migration capacity. Thus, identifying agents that can mitigate photoaging is crucial for developing skincare therapies. One of the alternative therapies currently being developed is plant-derived exosome-like nanoparticles (PDENs). Papaya (Carica papaya), renowned for its flavonoid and phenolic content, exhibits potent antioxidant properties and has been extensively utilized in skincare formulations. Therefore, this study investigates the effects of papaya-derived exosome-like nanoparticles (CP-PDEN) on inhibiting photoaging in 1BR3 fibroblast cell lines and zebrafish. Results indicated that CP-PDEN effectively internalized into 1BR3 cells and was categorized as nontoxic. The study then examined photoprotective effects after UVB irradiation (100 mJ/cm2) and found that CP-PDEN treatment resulted in better viability and nuclear morphology. Furthermore, CP-PDEN accelerates wound closure in 1BR3 cells and decreases melanocyte count in zebrafish compared with controls. In conclusion, CP-PDEN significantly enhanced cell viability and migration and suppressed melanogenesis, indicating its potential for anti-photoaging skincare applications.
RESTORASI MANGROVE BERBASIS KOMUNITAS PESISIR: PENYEMAIAN, MONITORING, DAN TANTANGAN EKOLOGI Rieuwpassa, Frets; Wibowo, Indra; Choesin, Devi Nandita; Gansalangi, Ferdinand; Tomasoa, Aprelia Martina; Sambeka, Yana; Nursatya, Safira Meidina; Wibowo, Arie; Balansa, Walter; Barlian, Anggraini
Jurnal Ilmiah Tatengkorang Vol 8 No 2 (2024): Jurnal Ilmiah Tatengkorang
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat, Politeknik Negeri Nusa Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54484/tkrg.v8i2.665

Abstract

As a collaborative effort between the School of Life Sciences (SITH) Institute Technology Bandung (ITB), Polnustar, and the people of Salurang, this community service aimed to restore the coastal area of Salurang village, Sangihe Islands, North Sulawesi, through various initiatives, including mangrove rehabilitation and sponge cultivation. This report specifically focuses on the propagation of 1,500 Rhizophora apiculata propagules to combat severe sedimentation from local mining and reclamation, which degraded the coastal area and reduced fish populations. Initial monitoring showed a 95.20% success rate for R. apiculata, later dropping to 78.87% due to plastic waste, animal disturbances, and tidal conditions. While the primary focus was on R. apiculata, the article also touched on the potential use of Calophyllum inophyllum, which successfully grew on different media for future restoration efforts. The monitoring process involved Polnustar and local residents tracking growth and survival every 30 days, with SITH ITB conducting more thorough checks 60 days after propagation. These activities, including guest lectures and hands-on training, significantly enhanced local conservation knowledge and environmental stewardship. The findings highlight the potential of mangrove restoration in Salurang village, demonstrating that coastal restoration is achievable with proper management, adaptive strategies, and active community engagement. Sebagai bagian dari kolaborasi antara Sekolah Ilmu dan Teknologi Hayati (SITH) Institut Teknologi Bandung (ITB), Polnustar, dan masyarakat Salurang, kegiatan pengabdian masyarakat ini bertujuan untuk memulihkan wilayah pesisir desa Salurang, Kepulauan Sangihe, Sulawesi Utara, melalui berbagai inisiatif, termasuk rehabilitasi mangrove dan budidaya spons. Artikel ini secara khusus berfokus pada propagasi 1.500 propagul Rhizophora apiculata untuk mengatasi sedimentasi berat akibat penambangan lokal dan reklamasi, yang telah merusak wilayah pesisir dan mengurangi populasi ikan. Pemantauan awal menunjukkan tingkat keberhasilan R. apiculata sebesar 95,20%, yang kemudian menurun menjadi 78,87% akibat sampah plastik, gangguan hewan, dan kondisi pasang surut. Meskipun fokus utama adalah pada R. apiculata, artikel ini juga menyinggung potensi penggunaan Calophyllum inophyllum yang berhasil tumbuh pada media yang berbeda untuk upaya restorasi di masa depan. Proses pemantauan melibatkan Polnustar dan warga setempat dalam memantau pertumbuhan dan kelangsungan hidup propagul setiap 30 hari, dengan SITH ITB melakukan pemeriksaan lebih mendalam 60 hari setelah propagasi. Kegiatan ini, termasuk kuliah tamu dan pelatihan langsung, secara signifikan meningkatkan pengetahuan konservasi lokal dan pengelolaan lingkungan. Hasil pengabdian masyarakat ini mengindikasikan potensi restorasi mangrove di desa Salurang, menunjukkan bahwa pemulihan pesisir berpotensi dicapai dengan manajemen yang tepat, strategi adaptif, dan keterlibatan aktif masyarakat
Quantitative Analysis of hsa-miR-21-5p Expression in Human Umbilical Cord Mesenchymal Stem Cell-derived Exosomes Under Hypoxia Muhammad, Daisy Ramadhani; Barlian, Anggraini; Murti, Harry; Prawira, Matheus Alvin; Faza, Naufalia
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1426-1436

Abstract

Exosomes have emerged as a cell-free alternative to mesenchymal stem cells (MSCs). Hypoxic preconditioning might result in the production of exosomes with ideal properties. As such, the effect of hypoxia on hsa-mir-21-5p expression, which is known to contribute to the immunomodulatory activity of exosomes, should be investigated. This research aimed to enrich and characterize exosomes from human umbilical cord MSC (hUC-MSC) secretome samples secreted under hypoxia and normoxia, as well as to analyze the expression of hsa-miR-21-5p in each sample. Secretomes were collected from cultured cells under both hypoxic and normoxic conditions, and exosomes were subsequently enriched from the secretomes through ultrafiltration. In this research, it was found that particles in N-Exo and H-Exo exhibited round morphology, expressed exosomal CD81 and CD63 markers with an average size of 153.7 ± 31.3 nm and 137.7 ± 25.0 nm, respectively. N-Exo and H-Exo can also be internalized by mesenchymal stem cells (MSCs). qRT-PCR results then indicated that hsa-miR-21-5p expression was significantly lower in H-Exo (p < 0.05). In conclusion, hypoxic preconditioning, as performed in this research, was also found to affect hsa-miR-21-5p and downregulate its expression in hypoxic samples relative to controls.
Isolation and Characterization of Plant-Derived Exosome-Like Nanoparticles (PDEN) from Sapodilla Fruit (Manilkara zapota) as an Antioxidant Agent in 1BR3 Cell Line Noeng, Anastasia; Barlian, Anggraini
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1654-1662

Abstract

Plant-derived exosome-like nanoparticles (PDENs) have emerged as promising natural nanocarriers with potential applications in biomedicine and cosmetics due to their biocompatibility, stability, and intrinsic bioactivity. The objective of this research is to isolate, characterize, and analyze the effects of MZ-PDENs on 1BR3 cells. MZ-PDENs were isolated using filtration, differential centrifugation, and polymer-based precipitation. MZ-PDENs were characterized for their physiochemical properties, size, shape, and antioxidant activity. In vitro, bioactivities were conducted by measured cytotoxicity, proliferation assays, UVB protection, and migration assays. Results showed that MZ-PDENs possess strong antioxidant properties, are efficiently internalized by cells, and promote skin cell regeneration after UVB-induced damage. These findings suggest that MZ-PDENs have the potential as antioxidant agents for cosmeceutical applications and warrant further investigation in vivo models.
Co-Authors ., Rukisah Aprelia Martina Tomasoa, Aprelia Martina Arie Wibowo Balansa, Walter Devi Nandita Choesin, Devi Nandita Farid, Faticha Nasharo Qisthia Faza, Naufalia Gansalangi, Ferdinand Harry Murti Muhammad, Daisy Ramadhani Noeng, Anastasia Nursatya, Safira Meidina Prawira, Matheus Alvin Rieuwpassa, Frets Simanjuntak, Ricky Febrinaldy Wibowo, Indra Yana Sambeka