Article Per Year (5 Year)
p-Index From 2021 - 2026
2.959
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Science and Technology Indonesia Jurnal Ilmu Kefarmasian Indonesia Gema Wiralodra Jurnal Kefarmasian AKFARINDO Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Indonesian Journal of Pharmaceutical Education Journal of Social Research Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Ruang Cendekia : Jurnal Pengabdian Kepada Masyarakat Sciences of Pharmacy Jurnal Ilmiah Farmasi Simplisia Journal of Natural Product for Degenerative Diseases
RAHMAT, DENI
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 19 Documents
Search

 1   2 
SKRINING FITOKIMIA DAN PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK BUAH OKRA (Abelmoschus esculentus L.) Panca, Pra Panca Bayu Chandra; Ratih Laksmitawati, Dian; Rahmat, Deni
Jurnal Kefarmasian Akfarindo Vol 7 No 2 (2022)
Publisher : Akademi Farmasi Indonesia Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37089/jofar.vi0.149

Abstract

Okra (Abelmoschus esculentus L.) merupakan salah satu tanaman yang digunakan sebagai sayuran. Bagian tanaman yang sering digunakan adalah buah. Buah okra mengandung flavonoid yang berpotensi sebagai bahan alam yang berkhasiat sebagai obat. Penelitian tentang skrining fitokimia dan penetapan kadar flavonoid total dari ekstrak buah okra (Abelmoschus esculentus L.) telah dilakukan. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan kadar flavonoid total ekstrak buah okra. Ekstraksi ini menggunakan metode maserasi pelarut etanol 70 %. Hasil ekstraksi maserasi kemudian dilakukan proses penguapan hingga diperoleh ekstrak kental. Ekstrak kental lalu di identifikasi secara kualitatif menggunakan pereaksi kimia. Hasil identifikasi kualitatif diperoleh bahwa ekstrak buah okra mengandung flavonoid, alkaloid, saponin, tannin, triterpenoid, kumarin, fenolik dan glikosida. Kandungan flavonoid total ditetapkan menggunakan metode kolorimetri-Aluminium Klorida dengan instrumen spektrofotometri UV-Vis diperoleh kadar flavonoid total ekstrak buah okra sebesar 319.18 mg/100 gram dengan nilai SD 0.18.
AKTIVITAS GEL EKSTRAK BUAH OKRA (Abelmoschus esculentus L.) TERHADAP LUKA MENCIT HIPERGLIKEMIK BERDASARKAN JUMLAH INFILTRASI NEUTROFIL Pra Panca Bayu Chandra; Ratih Laksmitawati, Dian; Rahmat, Deni
Jurnal Kefarmasian Akfarindo Vol 8 No 1 (2023)
Publisher : Akademi Farmasi Indonesia Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37089/jofar.v8i1.169

Abstract

Luka diabetes akan mengalami fase inflamasi yang lebih lama dibandingkan luka normal, terjadinya gangguan peredaran darah besar dan kecil mengakibatkan sirkulasi darah menjadi kurang optimal, pemberian nutrisi dan oksigenasi berkurang sehingga proses penyembuhan luka diabetes menjadi lebih lama. Penelitian ini bertujuan untuk melihat aktivitas gel ekstrak buah okra (Abelmoschus esculentus L.) pada luka mencit dengan kondisi hiperglikemik berdasarkan indikator histopatologi yaitu jumlah infiltrasi neutrofil. Mencit jantan galur Mus musculus dikondisikan hiperglikemik dengan STZ dosis 0,06 mg/gBB. Mencit dibagi menjadi 8 kelompok (n=6). Kelompok 1 (Kontrol Non-DM), kelompok 2 (Kontrol DM), kelompok 3 sampai 8 (Uji). Dosis glibenclamide 5 mg/KgBB, dosis ekstrak buah okra 400 mg/KgBB serta dosis CMC Na 5 mL/KgBB. Terapi diberikan 1 kali sehari (oral dan topikal) selama 15 hari terapi yang dilihat pada hari ke-0, 5, 11 dan 15. Hasil penelitian jumlah infiltrasi neutrofil mengalami penurunan pada kelompok uji dibandingkan kelompok kontrol. Pemberian kombinasi gel ekstrak buah okra secara topikal dengan ekstrak buah okra secara oral selama 15 hari terapi mampu menurunan radang neutrofil dibandingkan dengan pemberian tanpa kombinasi atau hanya secara oral. Pemberian kombinasi antara topikal gel dan oral ekstrak buah okra mempercepat proses penyembuhan luka pada mencit hiperglikemik dibandingkan pemberian non kombinasi. Manajemen kadar glukosa darah puasa berpengaruh dalam keberhasilan proses penyembuhan luka diabetes.
Evaluation of Total Flavonoid Content, Xanthine Oxidase Inhibition, and Uric Acid Inhibitory Activity In Vitro and In Vivo of Ethanol Extract of Sida rhombifolia Aulena, Desi Nadya; Kumala, Shirly; Abdillah, Syamsudin; Rahmat, Deni; Sugiastuti, Fenty; Indarwati, Indarwati; Fitriyani, Dwi
Sciences of Pharmacy Volume 3 Issue 3
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0303255

Abstract

Secondary metabolites content in Sida rhombifolia L. (SR) are believed to inhibit the xanthine oxidase (XO) enzyme, which is responsible for converting hypoxanthine and xanthine into uric acid. This study aimed to compare flavonoid levels, XO inhibitory activity, and the reduction of uric acid levels of sidaguri herb, stem, leaves, and root extract both in vitro and in vivo. The research results showed that the highest total flavonoid content in SR was found in the leaves, at 21.29±0.08 mg/QE/g extract. The IC50 values were as follows: roots (EESRR 1096.07±1.07 ppm), stems (EESRS 561.62±7.01 ppm), leaves (EESRL 101.84±0.63 ppm), and herbs (EESRH 104.70±3.50 ppm). EESR can inhibit the XO enzyme and has potential as an anti-hyperuricemia agent. The best total flavonoid content and IC50 values were observed in EESRL, which are 21.29±0.08 mg/QE/g and 101.84±0.63 ppm, respectively. EESRL at a dose of 400 mg/kg BW has the equivalent ability to reduce blood uric acid levels in mice when compared to the positive control group. Based on these findings, it can be concluded that EESRL has significant potential as a natural XO inhibitor and can be considered a promising candidate for the development of anti-hyperuricemia treatments.
Physical-chemical stability test on spray gel with active compound ethyl p-methoxycinnamate (Kaempferia galanga Linn.) and menthol, using combination gelling agent na cmc and copovidone Suryani, Nelly; Komala, Ismiarni; Akhsani, Luthfia Wikhdatul; Dahlizar, Sabrina; Betha, Ofa Suzanti; Rahmat, Deni
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 2 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i2.1626

Abstract

The major component of galangal rhizome (Kaempferia galanga Linn) is ethyl p-methoxycinnamate (EPMS), which amounts to 80.05%. Significant anti-inflammatory activity is exhibited by EPMS from galangal rhizome through the inhibition of carrageenan-induced mouse edema and the inhibition of IL-6 production. EPMC is recognized for various pharmacological effects, particularly its potential as a wound healing agent due to its anti-inflammatory properties. The choice of spray was made because it meets the characteristics of anti-inflammatory preparations in the wound healing process. The preparation must be moist, easily adjusted to the shape of the wound (flexible), sterile, and evenly distributed, covering the tissue, and can be easily removed from the tissue, including the wound. A spray gel dosage form was formulated with varying concentrations of the gelling agent Na CMC, F1 (0.5%), F2 (1.0%), and F3 (1.5%). Physical stability was evaluated at room temperature storage on days 0, 7, 14, and 21. Organoleptics, homogeneity, viscosity, pH, spray pattern, adhesive spread ability, centrifugation test, and cycling test were evaluated. Chemical stability evaluation was carried out using UV-Vis spectrophotometry to assess the EPMS levels in the preparation. The results of the physical evaluation showed that all formulas were stable in terms of organoleptics, homogeneity, and pH, which was in the range of 6.6–7.1; the weight per spray was uniform and relatively stable in centrifugation and cycling tests. The use of a combination of sodium carboxymethylcellulose and copovidone can produce a spray gel preparation of a good aromatic ginger (Kaempferia galanga Linn) crystal with a 1%.
Quality Parameter Analysis, Antioxidant Activity, and FTIR Profile of Turmeric (Curcuma longa L.) from Medan, Sumba, and Papua Noordam, Errol Rakhmad; Rahmat, Deni; Sandhiutami, Ni Made Dwi; Yuliana, Nancy Dewi
Indonesian Journal of Pharmaceutical Education Vol 5, No 1 (2025): January–April 2025
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v5i1.30494

Abstract

Turmeric (Curcuma longa L.) has long been used as a medicinal plant because it contains bioactive compounds with various health benefits. This research aims to analyze the quality parameters, antioxidant activity and Fourier Transform Infrared (FTIR) spectrum of turmeric originating from Medan, Sumba and Papua. The water content test shows that turmeric extract from Sumba has a water content of 8.55%, better than extracts from Medan and Papua. The highest yield was found in turmeric from Sumba (18.55%). Antioxidant activity testing shows that turmeric extract from Sumba has the strongest antioxidant activity with an IC50 value of 23.74 ppm. FTIR analysis shows the presence of hydroxyl groups (O-H) in the wave number range of 3500-3200 cm⁻¹ and C-H bonds at 3000-2800 cm⁻¹ in all samples. The results of this research show that turmeric from Sumba has better quality than turmeric from Medan and Papua.
The Antiaging Potential of Serum Formulations from Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA on Injured Human Fibroblast Cells Girsang, Ermi; Wargasetia, Teresa Liliana; Rahmat, Deni; Gondokesumo, Marisca Evalina; Harjanti, Mathelda Weni; Widowati, Wahyu; Zahiroh, Fadhilah Haifa; Saufa, Zahra Qisthi; Takasenserang, Oktaviana; Hadiprasetyo, Dhanar Septyawan
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.623-631

Abstract

External aging factors such as UV exposure, pollution, and lifestyle choices contribute to skin aging, resulting in deep wrinkles, fine lines, and rough skin, which can lower self-confidence. Plant extracts have been widely studied for their antiaging potential, while Salmon DNA has shown promise in stimulating collagen production. This study explores the formulation of a serum combining Centella asiatica, Curcuma longa, Aloe vera, Rosa centifolia, and Salmon DNA for its antiaging effects on injured human fibroblast cells. The serum was formulated using extracts from C. asiatica, C. longa, A. vera, R. centifolia, and Salmon DNA. Antioxidant activity was evaluated with the DPPH method, cytotoxicity using the WST-8 assay, and gene expression through qRT-PCR for COL1A1, TGF-β1, HYAL-1, and FGF-2. The serum exhibited weak antioxidant activity (IC50 = 373.33 µg/ml) and reduced cell viability at high concentrations. Gene expression analysis revealed increased expression of COL1A1, TGF-β1, and FGF-2, along with reduced HYAL-1 expression in injured BJ cells. The formulated serum shows potential as an antiaging agent, promoting collagen production and reducing hyaluronidase activity.
Toxicity of Sida rhombifolia L. 96% ethanol extract based on LD50 and macropathological examination of mice's organs Aulena, Desi Nadya; Kumala, Shirly; Abdillah, Syamsudin; Rahmat, Deni; Zaidan, Sarah; Fitriyani, Dwi; Raihan, Dany
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 1 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i1.1463

Abstract

The Indonesian people empirically use Sidaguri (Sida rhombifolia L.) in medicine, such as anti-hyperuricemia. Herbal-based treatment is currently much preferred. Drug metabolism, in general, mainly occurs in the liver, so the possibility of damage to this organ is high. This study aims to determine the acute toxicity category of 96% ethanol extract of sidaguri based on the LD50 value, changes in body weight, changes in organ weight, and macro pathology in the organs of male DDY mice in vivo. The method used in this research was experimental with a fixed dose design following the Indonesian Food and Drug Authority regulations regarding guidelines for in vivo non-clinical toxicity tests. The test group was divided into six dose groups (standard, 50, 300, 2000, 5000, and 15000 mg/kg Body of weight). Observations were made for 24 hours. Observations were continued for 14 days on death parameters, toxicity symptoms, body weight, and relative organ weights using five main organs (heart, lungs, liver, spleen, and kidneys, and pathological examination). The acute toxicity test results showed no death in all dose treatment groups. Macropathological analysis did not show abnormalities in organs in all groups of mice. LD50 value is more than 15000 mg/kg. Sidaguri 96% ethanol extract is safe and Practically non-toxic.
EDUKASI DAN PEMERIKSAAN KULIT: UPAYA MENINGKATKAN KESADARAN MASYARAKAT TENTANG PERAWATAN KULIT Widowati, Wahyu; Wargasetia, Teresa Liliana; Elisabeth, Mariska; Haryanti, Mathelda Weni; Rahmat, Deni; Girsang, Ermi; Gondokesumo, Marisca Evalina; Nindya, Faradhina Salfa; Triharsiwi, Dwi Nur
Jurnal Abdimas Ilmiah Citra Bakti Vol. 6 No. 1 (2025)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v6i1.4471

Abstract

Penuaan kulit merupakan proses alami yang tidak dapat dihindari oleh setiap individu. Proses penuaan ini dapat dipengaruhi secara intrinsik maupun ekstrinsik. Faktor penuaan ekstrinsik yang berlebih dapat memberikan pengaruh signifikan pada kesehatan kulit. Di saat bersamaan, sejumlah masyarakat belum mengetahui pentingnya menjaga kualitas kulit. Maka dari itu, bersama dengan Universitas Kristen Maranatha, diselenggarakan penyuluhan untuk meningkatkan kesadaran penjagaan kualitas kulit hingga memperoleh kulit yang optimal. Kegiatan ini juga dilengkapi dengan pemeriksaan kulit menggunakan Skin Analyzer, serta pemberian skincare berbasis ekstrak bahan alam. Penyuluhan disampaikan dengan metode ceramah yang dilengkapi dengan presentasi visual. Kegiatan yang dilaksanakan dievaluasi melalui pengisian formulir evaluasi oleh seluruh peserta. Kegiatan penyuluhan ini dihadiri oleh 129 orang dengan mayoritas peserta berasal dari kelompok usia dewasa. Hasil formulir evaluasi penyuluhan menunjukkan bahwa mayoritas peserta (>50%) menilai bahwa sistematika penyuluhan, isi penyuluhan, organisasi, dan media yang digunakan selama kegiatan tergolong sangat baik. Hasil formulir evaluasi produk menunjukkan lebih dari 60% peserta yang mengikuti pemeriksaan kulit dapat menilai bahwa tekstur, kemasan, kandungan, kualitas, informasi produk, aroma, dan warna dari produk perawatan kulit berada dalam kategori sangat baik. Hasil formulir penilaian menunjukkan tujuan dari penyuluhan telah tercapai, yaitu kemampuan dalam pemahaman terkait karakteristik produk perawatan kulitdan memperoleh respons baik dari peserta. Setelah penelitian ini diharapkan masyarakat dapat lebih selektif dalam menggunakan produk perawatan kulit.
ANALISIS PREDIKSI EFEK ANTIINFLAMASI PADA PIPER PORPHYROPHYLLUM SECARA IN SILICO Prayoga, Tria; Rahmat, Deni; Sandhiutami, Ni Made Dwi; Desmiaty, Yesi
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 10 No 1 (2025): Jurnal Ilmiah Ibnu Sina
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jiis.v9i2.2352

Abstract

This study aims to predict the anti-inflammatory effects of compounds in Piper porphyrophyllum using in silico methods. The compounds were analyzed using AutoDock software to evaluate their binding potential with the target protein and Lipinski's rule of five to assess their drug-likeness based on pharmacokinetic properties. Qualitative results show that compounds such as 4'-hydroxy-3',5,7-trimethoxyflavone, 4',5-dihydroxy-3',7-dimethoxyflavone, and 5-hydroxy-3',4',7-trimethoxyflavone form significant interactions with key residues on the target protein. Quantitatively, these compounds exhibit more negative binding free energy compared to the natural ligand (-7.34 kcal/mol), with values of -8.35 kcal/mol, -8.13 kcal/mol, and -8.20 kcal/mol, respectively, indicating stronger binding affinity. Evaluation using Lipinski's rule shows that these compounds meet the criteria as potential drug candidates. In conclusion, this in silico method can predict the potential of Piper porphyrophyllum compounds as anti-inflammatory agents, supporting further research for experimental validation.
In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum Aji, Nur; Kumala, Shirly; Mumpuni, Esti; Rahmat, Deni
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Propionibacterium acnes (P. acnes), a symbiotic bacterium involved in the development of acne, is recognized for its capacity to develop resistance due to its ability to generate biofilms. According to a previous investigation, the ethyl acetate fraction derived from Zingiber montanum exhibited anti-bacterial properties against P. acnes. Nevertheless, the causative role of biofilm formation in its resistance was not previously recognized. This study aims to ascertain the antibiofilm efficacy using in silico and in vivo methodologies. In this study, in silico examination utilized molecular docking to inhibit the quorum sensing mechanism by blocking the autoinducer 2 (AI-2) signal. The target protein was AI-2 binding periplasmic protein LuxP (PDB code: 4YRZ). Following that, an in vitro test was conducted using the crystal violet staining method, which was read using a microplate reader at a wavelength of 600 nm. The readings were expressed as percent inhibition and IC50. The in silico results showed that there were six compounds in the ethyl acetate fraction that have the potential to inhibit AI-2 signaling. Curcumin showed the highest inhibitory activity. Meanwhile, the in vitro results revealed that the ethyl acetate fraction of Z. montanum obtained the minimum biofilm inhibitory concentration (MBIC) of 2.5 % (w/v) and IC50 = 0.28 % (w/v). It can be concluded that the ethyl acetate fraction of Zingiber montanum could inhibit the biofilm formation of P. acnes.  
Co-Authors . Syamsudin Ahmad Faried Aji, Nur Akhsani, Luthfia Wikhdatul Aulena, Desi Nadya Azis, Rizal Chaidir Chaidir Datubara, Martaulina Elisabet Dian Ratih Laksmitawati Didik Priyandoko Djami, Ratna Djamil, Ratna Dwi Fitriyani, Dwi Effionora Anwar Elisabeth, Mariska Errol Rakhmad Noordam Esti Mumpuni, Esti Girsang, Ermi Hadiprasetyo, Dhanar Septyawan Harjanti, Mathelda Weni Harry Murti Haryanti, Mathelda Weni Hermawati, Ema Indarwati Indarwati Jeffrey ., Jeffrey Komala, Ismiarni Marisca Evalina Gondokesumo, Marisca Evalina Nainggolan, Ita Margaretha Nancy Dewi Yuliana Ni Made Dwi Sandhiutami Nindya, Faradhina Salfa Ofa Suzanti Betha Panca, Pra Panca Bayu Chandra Pra Panca Bayu Chandra Prayoga, Tria Puspitasari, Ranny Putra, Riza Dharma Qlintang, Sandy Raihan, Dany Sabrina Dahlizar Saufa, Zahra Qisthi Shirly Kumala Sinuhaji, Indra Sofa Fajriah Sugiastuti, Fenty suryani, nelly Swandiny, Greesty F Swandiny, Greesty Finotory Syamsudin Syamsudin Takasenserang, Oktaviana Taurhesia, Shelly Teresa Liliana Wargasetia Triharsiwi, Dwi Nur Utami, Usi Hikmah Vinna Kurniawati Sugiaman, Vinna Kurniawati WAHYU WIDOWATI Yesi Desmiaty, Yesi Zahara, Indah Zahiroh, Fadhilah Haifa Zaidan, Sarah