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All Journal Indonesian Journal of Biotechnology Science and Technology Indonesia Jurnal Abdimas Ilmiah Citra Bakti (JAICB)
Triharsiwi, Dwi Nur
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Physics Social Sciences

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EDUKASI DAN PEMERIKSAAN KULIT: UPAYA MENINGKATKAN KESADARAN MASYARAKAT TENTANG PERAWATAN KULIT Widowati, Wahyu; Wargasetia, Teresa Liliana; Elisabeth, Mariska; Haryanti, Mathelda Weni; Rahmat, Deni; Girsang, Ermi; Gondokesumo, Marisca Evalina; Nindya, Faradhina Salfa; Triharsiwi, Dwi Nur
Jurnal Abdimas Ilmiah Citra Bakti Vol. 6 No. 1 (2025)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v6i1.4471

Abstract

Penuaan kulit merupakan proses alami yang tidak dapat dihindari oleh setiap individu. Proses penuaan ini dapat dipengaruhi secara intrinsik maupun ekstrinsik. Faktor penuaan ekstrinsik yang berlebih dapat memberikan pengaruh signifikan pada kesehatan kulit. Di saat bersamaan, sejumlah masyarakat belum mengetahui pentingnya menjaga kualitas kulit. Maka dari itu, bersama dengan Universitas Kristen Maranatha, diselenggarakan penyuluhan untuk meningkatkan kesadaran penjagaan kualitas kulit hingga memperoleh kulit yang optimal. Kegiatan ini juga dilengkapi dengan pemeriksaan kulit menggunakan Skin Analyzer, serta pemberian skincare berbasis ekstrak bahan alam. Penyuluhan disampaikan dengan metode ceramah yang dilengkapi dengan presentasi visual. Kegiatan yang dilaksanakan dievaluasi melalui pengisian formulir evaluasi oleh seluruh peserta. Kegiatan penyuluhan ini dihadiri oleh 129 orang dengan mayoritas peserta berasal dari kelompok usia dewasa. Hasil formulir evaluasi penyuluhan menunjukkan bahwa mayoritas peserta (>50%) menilai bahwa sistematika penyuluhan, isi penyuluhan, organisasi, dan media yang digunakan selama kegiatan tergolong sangat baik. Hasil formulir evaluasi produk menunjukkan lebih dari 60% peserta yang mengikuti pemeriksaan kulit dapat menilai bahwa tekstur, kemasan, kandungan, kualitas, informasi produk, aroma, dan warna dari produk perawatan kulit berada dalam kategori sangat baik. Hasil formulir penilaian menunjukkan tujuan dari penyuluhan telah tercapai, yaitu kemampuan dalam pemahaman terkait karakteristik produk perawatan kulitdan memperoleh respons baik dari peserta. Setelah penelitian ini diharapkan masyarakat dapat lebih selektif dalam menggunakan produk perawatan kulit.
Potential of Secretome Hydrogel for Wound Healing in LPS- and Scratch-Induced BJ Cells as an Inflammation Model Widowati, Wahyu; Rahmat, Deni; Faried, Ahmad; Nainggolan, Ita Margaretha; Priyandoko, Didik; Wargasetia, Teresa Liliana; Sugiaman, Vinna Kurniawati; Triharsiwi, Dwi Nur; Qlintang, Sandy; Murti, Harry; Azis, Rizal; Jeffrey, Jeffrey
Science and Technology Indonesia Vol. 10 No. 4 (2025): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.4.1242-1254

Abstract

Wound healing often requires specialized interventions to accelerate recovery due to prolonged inflammation and limited regenerative factors. Umbilical Cord Mesenchymal Stem Cells secretome (UCMSCs) comprises various cytokines and growth factors that can promote wound healing. This study aims to analyze the potential of a secretome-based hydrogel as a wound-healing agent using BJ fibroblast cells induced by lipopolysaccharide (LPS) and scratch injury as an inflammation model. The secretome hydrogel was formulated using Carbopol, Hydroxypropyl Methylcellulose (HPMC), Hydroxyethyl Cellulose (HEC), and secretome. Cytotoxicity was conducted using the WST-8 assay, while cell migration was evaluated through a scratch assay. Tumor Necrosis Factor-???? (TNF-????), Nuclear Factor kappa-B (NF-????B), and Interleukin-8 (IL-8) gene expression were analyzed via qRT-PCR. Additionally, malondialdehyde (MDA) levels were measured for oxidative stress assessment, whereas Connective Tissue Growth Factor (CTGF) and Transforming Growth Factor-????1 (TGF-????1) levels were quantified using ELISA and colorimetric assays. The secretome hydrogel exhibited no cytotoxic effects on BJ fibroblast cells and significantly enhanced cell migration. Moreover, it reduced the TNF-????, IL-8, and NF-????B expression, indicating anti-inflammatory activity. The hydrogel also decreased MDA levels while increasing TGF-β1 and CTGF expression, suggesting antioxidant properties and enhanced tissue regeneration in the inflammatory model. The secretome-based hydrogel presents a promising therapeutic approach for promoting chronic wound healing by modulating inflammation, reducing oxidative stress, enhancing tissue regeneration, and stimulating fibroblast migration.
Anti‐inflammatory properties of conditioned medium from human Wharton’s jelly mesenchymal stem cells Azis, Rizal; Widowati, Wahyu; Gunanegara, Rimonta Febby; Kusuma, Hanna Sari Widya; Triharsiwi, Dwi Nur; Hadiprasetyo, Dhanar Septyawan; Gondokesumo, Marisca Evalina; Mars, Nicholas
Indonesian Journal of Biotechnology Vol 30, No 4 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.107035

Abstract

Acute respiratory distress syndrome (ARDS) is a critical respiratory dysfunction triggered by intense in‐ flammation, microvascular damage, and increased epithelial and pulmonary vascular permeability. Human Wharton’s jelly mesenchymal stem cells (hWJMSCs) possess regenerative and anti‐inflammatory activities through the cytokines, chemokines, and growth factor secretion. The development of anti‐inflammatory agents derived from hWJMSCs has become one of the therapeutic solutions. Instead of direct cell use of hWJMSCs, their conditioned medium (CM) provides a cell‐free approach that delivers bioactive factors while minimizing the risks associated with stem cell transplantation. This study aims to measure the levels of vascular endothelial growth factor‐α (VEGF‐α), epidermal growth factor‐β (EGF‐β), interleukin‐10 (IL‐10), and hepatocyte growth factor (HGF) in CM‐hWJMSCs under non‐starvation and starvation conditions (24, 48 and 72 hours) using ELISA. The anti‐inflammatory potential of these factors was then analyzed through molecular docking with pro‐inflammatory cytokines. VEGF‐α, EGF‐β, IL‐10 and HGF levels were measured across all conditions. VEGF‐α ranged from 2590.37 to 3613.92 ng/mg protein; EGF‐β 347.01–504.43 ng/mg; IL‐10 302.59–729.28 pg/mg; and HGF 1747.20–2903.52 ng/mg. The molecular docking revealed strong binding between VEGF‐α, EGF‐β, IL‐10 and HGF with pro‐inflammatory cytokines, namely IL‐1β, IL‐6 and TNF‐α. VEGF‐α had the strongest bond with TNF‐α (–1162.3 kJ/mol), while EGF‐β formed the most hydrophobic and hydrogen interactions. The findings suggest that CM‐hWJMSCs, enriched with anti‐inflammatory and regenerative cytokines, may serve as a promising candidate for modulating the inflammatory pathways involved in ARDS pathogenesis. Longer starvation increased the secretion of VEGF‐α, EGF‐β, IL‐10 and HGF. These factors are known to promote angiogenesis, regulate immune responses, and protect against epithelial injury, thereby supporting the anti‐inflammatory and regenerative potential of hWJMSCs‐CM for ARDS therapy.
Co-Authors Ahmad Faried Azis, Rizal Didik Priyandoko Elisabeth, Mariska Girsang, Ermi Gunanegara, Rimonta Febby Hadiprasetyo, Dhanar Septyawan Hanna Sari Widya Kusuma, Hanna Sari Widya Harry Murti Haryanti, Mathelda Weni Jeffrey ., Jeffrey Marisca Evalina Gondokesumo, Marisca Evalina Mars, Nicholas Nainggolan, Ita Margaretha Nindya, Faradhina Salfa Qlintang, Sandy RAHMAT, DENI Teresa Liliana Wargasetia Vinna Kurniawati Sugiaman, Vinna Kurniawati WAHYU WIDOWATI