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Phenotypic Performance and The Characterization of Growth Hormone (GH|AluI) in Bangkok Chicken Breed M. Hari Setia Budi; Depison - Depison; Gushairiyanto Gushairiyanto
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 1 (2023): April 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.01.012

Abstract

This study aimed to determine Bangkok chicken’s phenotypic characteristics and growth hormone genes using the PCR-RFLP method. The samples used were 50 Bangkok chickens. Research in the field is taking phenotypic traits data. Research in the laboratory is carried out by DNA isolation, amplification, and restriction. This study showed that DOC weight, body weight, and bodyweight gain of DOC-1 month, 1-2 months, 2-3 months of Bangkok chickens were significantly different (P <0.05) higher than female Bangkok chickens. The results of the molecular analysis of DNA isolation were precise, then the amplification of the GH gene fragment with a product length of 997 bp and restriction obtained a ++ genotype. Allele frequencies and genotypes obtained in the Bangkok chicken GH|AluI genes are monomorphic. Conclusion: the phenotypic characteristics of the average body weight, the body weight gain of male Bangkok chicken was higher than that of female. Allele frequencies and genotypes of Bangkok chicken are monomorphic.
KARAKTERISTIK KUANTITATIF HASIL PERSILANGAN AYAM KAMPUNG SUPER DENGAN AYAM ARAB SAMPAI UMUR 2 BULAN M., SAMIUN; ., DEPISON; ., GUSHAIRIYANTO
Majalah Ilmiah Peternakan Vol 26 No 2 (2023): Vol. 26 No. 2 (2023)
Publisher : Fakultas Peternakan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MIP.2023.v26.i02.p06

Abstract

This study aims to determine the relationship of egg characteristics, quantitative characteristics of the crossing results of Super and Arabian chickens. The research material was 91 Supra chickens consisting of 43 males and 48 females. The method used was experimental observation method. Data collected on egg characteristics, BW, PBB, and body size were analyzed using t-test. The relationship between variables was analyzed by regression and corre- lation. T2-Hotelling analysis was used to compare the body size vectors of male and female Supra chickens. Prin- cipal Component Analysis was used to determine the determinants of the size and shape of male and female Supra chickens. The results of this study showed that the characteristics of eggs, body weight, body weight gain and body size of male Supra chickens were significantly different (P < 0.05) higher than female Supra chickens. Conclusion: The relationship between egg size and egg weight is positive, the closest correlation between sizes and egg weight is egg width. Body weight, body weight gain and body size of male Supra chickens were higher than females. The characteristics of body size in male and female Supra chickens are chest circumference, while the characteristics of body shape are wing length.
Perbandingan Daya Tunas, Daya Tetas, dan Karakteristik Kuantitatif Ayam KUB dengan Ayam Kampung (G1) Utama, Millisani; Depison, Depison; Gushairiyanto, Gushairiyanto; Ediyanto, Helmi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.16 KB) | DOI: 10.33772/jitro.v9i1.19736

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui perbandingan daya tunas, daya tetas dan karakteristik kuantitatif ayam KUB dengan ayam kampung (G1) sampai umur 3 bulan. Materi yang digunakan 315 butir telur dan 174 ekor ayam per galur dengan Metode penelitian eksperimen. Data yang dikumpulkan meliputi daya tunas, daya tetas, bobot badan, dan pertambahan bobot badan serta ukuran-ukuran tubuh. Data bobot badan, pertambahan bobot badan, dan ukuran-ukuran tubuh dianalisis menggunakan uji-t, sedangkan vektor rataan ukuran-ukuran tubuh dianalisis menggunakan uji T2-Hotelling. Penciri bentuk dan ukuran tubuh dianalisis menggunakan Analisis Komponen Utama. Hasil penelitian ini menunjukkan bahwa daya tunas ayam KUB dan ayam kampung yaitu 82,86% dan 81,59%, sedangkan daya tetas yaitu 71,26% dan 69,65%. Karakterisitik kuantitatif ayam KUB berbeda nyata p<0,05) lebih tinggi dibandingkan dengan ayam kampung. Penciri ukuran tubuh ayam KUB dan ayam kampung adalah lingkar dada, sedangkan penciri bentuk tubuh adalah panjang tubuh atas dan panjang sayap. Respon seleksi Ayam KUB dan ayam kampung yaitu 7 gr dan 9,3 gr. Kesimpulan: Ayam KUB dan ayam kampung memiliki daya tunas dan daya tetas yang relatif sama. Karakteristik kuantitatif ayam KUB lebih baik dibandingkan dengan ayam kampung. Ayam KUB dan ayam kampung memiliki respon seleksi yang positif.Keywords: Ayam KUB, ayam kampung, G1, respon seleksiComparison of Fertility, Hatchability and Quantitative Characteristics between KUB Chicken and Kampung Chicken (G1)ABSTRACTThis study aims to determine the comparison of fertility, hatchability, and quantitative characteristics of KUB chickens with kampung chickens (G1) 3 months of age. The material used is 315 eggs and 174 chickens per strain and using experimental methods. The data collected fertility, hatchability, body weight, body weight gain, and body measurements. Data on body weight, weight gain, and body measurements were analyzed using a t-test, while the mean vector of body measurements was analyzed using test T2-Hotelling. Body shape and size characteristics were analyzed using Principal Component Analysis. The results of this study showed the fertility of KUB and kampung chicken is 82.86% and 81.59%, while the hatchability is 71.26% and 69.65%. The quantitative characteristics of KUB chickens are significantly (p<0,05) higher than those of kampung chickens. The body size characteristics of KUB and kampung chickens are chest circumference, while the shape characteristics are back length and wing length. The selection response of KUB and kampung chicken is 7 g and 9.3 g. Conclusion: KUB chickens and kampung chickens have fertility and hatchability relative same. The quantitative characteristics of KUB chickens are better than of kampung chickens. KUB and kampung chickens have a positive selection response.Keywords: KUB chickens, kampung chickens, G1, selection responses
Determining the Matrilineal Origin of Indonesian Kerinci Duck Breed (Anas platyrhynchos) Based on MT-ND2 Gene Diversity Depison; Putra, Widya Pintaka Bayu; Gushairiyanto; Gunawan, Asep; Sumantri, Cece
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.581-588

Abstract

The Kerinci duck (Anas platyrhynchos), hailing from Jambi Province, represents one of the local duck breeds of Indonesia. This study aimed to ascertain the matrilineal origin of Kerinci ducks through analysis of the mitochondrial NADH Dehydrogenase subunit 2 (MT-ND2) gene. In this pursuit, forward sequences of the MT-ND2 gene (490 bp) were extracted from unsexed Kerinci ducks, totalling forty-eight (48) sequences, procured from blood samples. The findings demonstrated the identification of fifteen (15) distinct haplotypes within the MT-ND2 gene, with corresponding haplotype diversity (Hd) and nucleotide diversity (pi) values of 0.74 and 0.003, respectively. The resulting phylogenetic tree unveiled that Kerinci ducks exhibit two matrilineal origins: an Asian and an independent Kerinci lineage. Moreover, most Kerinci ducks were categorised within the H4 group (24 birds) of the Asian lineage. Nevertheless, this study also revealed the existence of an independent Kerinci lineage comprising eight (8) duck haplotypes. In conclusion, the analysis of the MT-ND2 gene underscored the genetic introgression of A. zonorhyncha and A. poecilorhyncha in Kerinci ducks.
Karakteristik Kuantitatif Sapi Bali Menggunakan Analisis Komponen Utama di Kabupaten Merangin dan Muaro Jambi, Provinsi Jambi Gushairiyanto, Gushairiyanto; Depison, Depison
Jurnal Sain Peternakan Indonesia Vol 16 No 1 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.1.74-79

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Tujuan penelitian ini, untuk mengetahui karakteristik kuantitatif sapi Bali menggunakan analisis komponen utama di Kabupaten Merangin dan Muaro Jambi Provinsi Jambi. Data yang dihimpun adalah karakteristik kuantitatif meliputi; bobot badan, pertambahan bobot badan, panjang badan, tinggi pundak, lingkar dada, dalam dada, lebar dada, tinggi pinggul, lingkar kanon dan lebar pinggul sapi Bali di kedua lokasi. Materi penelitian ini adalah sapi Bali Umur I1 (1-2) tahun. Metode penelitian survei, teknik pengambilan sampel secara purpossive sampling. Jumlah sampel yang dihimpun sebanyak 120 ekor masing masing kabupaten 60 ekor yang terdiri dari 30 ekor jantan dan 30 ekor betina. Uji t digunakan untuk menganalisis perbedaan karakteristik kuantitatif. Analsisi Komponen Utama digunakan untuk menentukan faktor penentu ukuran dan bentuk sapi Bali.  Hasil penelitian menunjukkan bahwa bobot badan, pertambahan bobot badan dan ukuran tubuh sapi Bali jantan dan betina antara kedua kabupaten berbeda tidak nyata (P>0,05), namun bobot badan, pertambahan bobot dan dan ukuran ukuran tubuh sapi Bali Jantan dan betina di kedua kabupaten berbeda nyata (P<0,05). Faktor penentu ukuran tubuh sapi Bali di Kabupaten Merangin dan Kabupaten Muaro Jambi adalah Lingkar Dada, sedangkan penentu bentuk tubuh adalah panjang badan. 
PEMANFAATAN MINERAL Zn-Cu ORGANIK UNTUK MEMACU PERTUMBUHAN SAPI BALI PENGGEMUKAN PADA KELOMPOK TANI DI DESA TANGKIT Musnandar, Endri; Syafwan, Syafwan; Muthalib, Muthalib; Gushairiyanto, Gushairiyanto
Jurnal Karya Abdi Masyarakat Vol. 1 No. 1 (2017): Jurnal Karya Abdi Masyarakat
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.132 KB) | DOI: 10.22437/jkam.v1i1.3728

Abstract

Pengabdian kepada masyarakat ini bertujuan untuk meningkatkan kinerja pertumbuhan sapi Bali yang dipelihara oleh peternak di desa Tangkit Kecamatan Sungai Gelam. Di desa ini terdapat kelompok tani yang memelihara sapi Bali untuk tujuan penggemukan akan tetapi kinerja pertumbuhannya masih sangat rendah yaitu berkisar 0,23 kg/ekor/hari. Kondisi ini disebabkan pemberian pakan yang hanya seadanya yaitu berupa hijauan saja tanpa diberikan pakan tambahan. Sementara itu, hasil penelitian Afzalani, dkk. (2012) mengemukakan bahwa pemberian pakan tambahan berupa suplementasi ampas tahu dan mineral Cu-organik dapat meningkatkan kinerja pertumbuhan sapi Bali yang diberikan pakan berbasis rumput rawa. Dikemukakan juga bahwa salah satu penyebab rendahnya produksi sapi di Indonesia bukan hanya kekurangan energi dan mineral tetapi yang lebih penting lagi adalah kekurangan mineral. Ampas tahu dan mineral Cu-organik akan menutupi kekurangan nutrisi pakan pada sapi yang diberi pakan berbasis rumput rawa. Ampas tahu adalah berupa sisa hasil pembuatan tahu, ketersediaannya cukup banyak disekitar desa Tangkit karena terdapat beberapa perusahaan tahu di sekitar desa, begitu pula Cu mudah diperoleh di toko kimia di sekitar perbatasan desa. Kondisi ini tentu kurang ideal dimana sapi kekurangan nutrisi tambahan sementara bahan baku pakan tambahan banyak tersedia. Oleh karena itu, teknologi suplementasi ampas tahu dan mineral Cu-organik untuk pakan sapi merupakan solusi yang tepat untuk mengatasi masalah ini. Sasaran kegiatan adalah petani yang mengusahakan penggemukan sapi Bali yaitu petani anggota kelompok tani “Sumber Makmur”. Metode yang digunakan pada kegiatan ini adalah gabungan atau perpaduan antara metode penyuluhan dan praktek pembuatan mineral Cu-organik. Diharapkan dari kegiatan ini akan dihasilkan mineral Cu-organik berbasis ampas tahu dan dicapai kinerja pertumbuhan sapi Bali yang lebih optimum di KelompokTani Rukun Makmur, Desa Tangkit.
Pelatihan Budidaya Ikan di dalam Ember Secara Aquaponik (BUDIKDAMBER) di Desa Muntialo Kecamatan Betara Kabupaten Tanjung Jabung Barat Lisna, Lisna; Arfiana, BSM; Ramadan, Fauzan; Gushairiyanto, Gushairiyanto; Sumadja, Wiwaha A
Journal of Rural and Urban Community Empowerment Vol. 6 No. 2 (2025): April
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jruce.6.2.156-161

Abstract

Kelompok Wanita Tani Desa Muntialo merupakan salah satu desa yang terletak di Kecamatan Betara Kabupaten Tanjung Jabung Barat. Kelompok Wanita Tani merupakan kelompok yang terdiri dari Ibu rumah tangga hanya mengandalkan penghasilan dari suami. Upaya yang dilakukan untuk mencukupi gizi yang dibutuhkan saat terjadinya kenaikan pangan satunya adalah melakukan budidaya ikan yang cukup simpel hanya menggunakan ember sebagai media dan tidak membutuhkan lahan yang luas yaitu Budikdamber ikan lele secara aquaponik. Tujuan dari pengabdian masyarakat ini adalah untuk memberikan pengetahuan kepada masyarakat yang ada di Desa Muntialo tentang bagaimana cara melakukan pembudidaya ikan dengan ember secara aquaponik dengan memanfaatkan perkarangan rumah sekitar. Kegiatan ini dilaksanakan pada 5 Oktober 2024 di Desa Muntialo Kecamatan Betara. Metode yang digunakan dalam kegiatan ini adalah Sosialisasi dan Demplot (Demonstrasi Plot) ke Kelompok Wani Tani. Hasil kegiatan kelompok wanita tani sangat antusias dalam mengikuti kegiatan karena mampu membuat budidaya yang sederhana tanpa mengunakan lahan yang luas. Adapun kesimpulan dari kegiatan ini adalah Kelompok Wanita Tani mendapatkan hal yang baru agar daapt di terapkan di rumah sendiri untuk membudidayakan ikan dan menanam sayur-sayuran untuk memenuhi kebutuhan sehari-hari serta saran yang diberikan kepada TIM agar dapat melakukan kegiatan pembuatan pakan ikan
Association of Gene Growth Hormone (GH) Diversity with Quantitative Characteristics of Crossing Merawang Chickens with Arabic Chickens Muhammad, Mus'ab; Depison; Gushairiyanto; Harahap, Ratna Sholatia
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.2

Abstract

The growth hormone (GH) gene plays a crucial role in regulating the growth and metabolism of Chickens. This study investigates the polymorphism of the GH gene and its association with growth traits in chickens resulting from the crossbreeding of Merawang and Arabs (MeA). The research began by raising 60 chickens—30 males and 30 females—from the Merawang and Arabic chicken cross for four months. Various growth traits, including weight, weight gain, and body size, were measured. Blood samples from the MeA chickens were collected for DNA analysis to assess GH gene polymorphism. The analysis involved DNA extraction, PCR amplification, and RFLP using the Taql enzyme. The data were analysis using T-test to evaluate the association of the GH gene polymorphism with growth traits. The results showed that the male of crosses between Merawang and Arab chicken (MeA) have higher significant (P<0.05) growth traits, including body weight, weight gain, and body size than female MeA chickens. The chest circumference is a defining characteristic of body size in male and female MeA chickens, while the upper body length is a crucial feature of body shape. The GH gene were found polimorpic with three genotypes, including GG, AG, and AA. The GH gene polymorphisms have a significant association (P<0.05) with growth traits, including body weight, body weight gain, and body size. Chicken with GG genotype have a higher growth trait than others. The GH gene can be used as a potential genetic marker for selection for improving chicken growth.
The Relationship Between the Diversity of Growth Hormone Genes and the Body Weight of Sentul Chickens Pratama, Shera Aditia; Depison; Gushairiyanto, Gushairiyanto
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 3 (2023): December 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.03.05

Abstract

This study aims to determine the diversity of growth hormone genes in Sentul chickens using PCR-RFLP. This study used 70 samples of Sentul chicken blood (35 males and 35 females). The research methods are Bodyweight and body weight gain data collection, bloodcollection, DNA extraction, and PCR-RFLP. Polymorphism analysis included genotype and allele frequencies, Hardy-Weinberg balance, and heterozygosity. The difference in the average body weight against the genotype of the GH|Msp1 gene was tested for the average difference (t-test). The average body weight of DOC - 3 months and body weight gain of DOC - 3 months in male Sentul chickens were significantly different (P <0.05) higher than female Sentul chickens. The GH|MspI gene in Sentul chickens is polymorphic. The Sentul chicken population was in Hardy-Weinberg equilibrium. Heterozygosity in the Sentul chicken population was Ho 0.228, greater than the He value of 0.213. Genotypes (++) and (+-) were significantly different (P<0.05) on body weight and body weight gain of Sentul chickens aged DOC - 3 months. The conclusion of this study, the allele frequency at the GH|MspI Sentul chicken is polymorphic with two genotypes, namely (++) and (+-), and there are two kinds of alleles (+) and (-). Chisquare test results on the GH|mspI Sentul chicken show balance Hardy-Weinberg. Heterozygosity in Sentul chickens showed diversity. The genotype of the GH|Msp1 Sentul chickens with the genotype (++) had higher body weight and body weight gain than those with the (+-) genotype
Growth Hormone Gene Diversity and Quantitative Characteristics of Thin-Tailed Sheep in Muara Tembesi District Batang Hari Regency Pratama, Bondan; Depison, Depison; Gushairiyanto, Gushairiyanto; Gunawan, Asep; Sumantri, Cece
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.13

Abstract

Research on thin-tailed sheep (TTS) via quantitative and molecular characterization is essential for obtaining primary data on genetic diversity. The quantitative description includes body weight, weight gain, and body measurements. Molecular characterization is performed directly on structural genes. One gene that affects livestock growth and metabolism is the growth hormone (GH) gene. The commonly used method is PCR-RFLP. The research was conducted in two stages. Field research was conducted to obtain quantitative characteristic data, including body weight, weight gain, and body measurements, as well as blood samples from 62 TTS (31 males and 31 females). Research in the laboratory includes DNA extraction, PCR amplification, and restriction with the Msp1 enzyme. Data analysis included a t test to calculate the differences in body weight, weight gain, and body size between male and female TTS. T2-hotelling was used to compare the morphometric body sizes of the male and female TTS simultaneously, and principal component analysis was used to determine the body shape and size characteristics of the male and female TTS. The results revealed that the male TTS mean body weight, weight gain, and body size were significantly (P<0.05) greater than those of the female TTS. The results of the molecular analysis of the GH gene with the MspI cutting enzyme revealed that it is monomorphic with the ++ genotype. Conclusion: Body weight, weight gain, and body size were greater in males with TTS than in females with TTS. The body size characteristics of male and female TTS patients were body length, shoulder height, and chest circumference. The body shape characteristic was chest depth. The restriction of the GH|Msp1 TTS gene DNA fragment in exon 5 in the Muara Tembesi district is monomorphic.