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All Journal HAYATI Journal of Biosciences Buletin Peternakan Jurnal Peternakan Sriwijaya Majalah Ilmiah Peternakan Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) JURNAL PETERNAKAN JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Tropical Animal Science Journal JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Journal of Rural and Urban Community Empowerment Livestock and Animal Research Jurnal Karya Abdi Masyarakat Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Agripet
Gushairiyanto Gushairiyanto
Animal Husbandry Study Program, Faculty Of Animal Husbandry, Jambi University Jl. Jambi-Ma. Bulian KM 15 Mendalo Darat Jambi 36361 Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Public Health Veterinary Other

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Association of Gene Growth Hormone (GH) Diversity with Quantitative Characteristics of Crossing Merawang Chickens with Arabic Chickens Muhammad, Mus'ab; Depison; Gushairiyanto; Harahap, Ratna Sholatia
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 1 (2025): April 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.01.2

Abstract

The growth hormone (GH) gene plays a crucial role in regulating the growth and metabolism of Chickens. This study investigates the polymorphism of the GH gene and its association with growth traits in chickens resulting from the crossbreeding of Merawang and Arabs (MeA). The research began by raising 60 chickens—30 males and 30 females—from the Merawang and Arabic chicken cross for four months. Various growth traits, including weight, weight gain, and body size, were measured. Blood samples from the MeA chickens were collected for DNA analysis to assess GH gene polymorphism. The analysis involved DNA extraction, PCR amplification, and RFLP using the Taql enzyme. The data were analysis using T-test to evaluate the association of the GH gene polymorphism with growth traits. The results showed that the male of crosses between Merawang and Arab chicken (MeA) have higher significant (P<0.05) growth traits, including body weight, weight gain, and body size than female MeA chickens. The chest circumference is a defining characteristic of body size in male and female MeA chickens, while the upper body length is a crucial feature of body shape. The GH gene were found polimorpic with three genotypes, including GG, AG, and AA. The GH gene polymorphisms have a significant association (P<0.05) with growth traits, including body weight, body weight gain, and body size. Chicken with GG genotype have a higher growth trait than others. The GH gene can be used as a potential genetic marker for selection for improving chicken growth.
The Relationship Between the Diversity of Growth Hormone Genes and the Body Weight of Sentul Chickens Pratama, Shera Aditia; Depison; Gushairiyanto, Gushairiyanto
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 3 (2023): December 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.03.05

Abstract

This study aims to determine the diversity of growth hormone genes in Sentul chickens using PCR-RFLP. This study used 70 samples of Sentul chicken blood (35 males and 35 females). The research methods are Bodyweight and body weight gain data collection, bloodcollection, DNA extraction, and PCR-RFLP. Polymorphism analysis included genotype and allele frequencies, Hardy-Weinberg balance, and heterozygosity. The difference in the average body weight against the genotype of the GH|Msp1 gene was tested for the average difference (t-test). The average body weight of DOC - 3 months and body weight gain of DOC - 3 months in male Sentul chickens were significantly different (P <0.05) higher than female Sentul chickens. The GH|MspI gene in Sentul chickens is polymorphic. The Sentul chicken population was in Hardy-Weinberg equilibrium. Heterozygosity in the Sentul chicken population was Ho 0.228, greater than the He value of 0.213. Genotypes (++) and (+-) were significantly different (P<0.05) on body weight and body weight gain of Sentul chickens aged DOC - 3 months. The conclusion of this study, the allele frequency at the GH|MspI Sentul chicken is polymorphic with two genotypes, namely (++) and (+-), and there are two kinds of alleles (+) and (-). Chisquare test results on the GH|mspI Sentul chicken show balance Hardy-Weinberg. Heterozygosity in Sentul chickens showed diversity. The genotype of the GH|Msp1 Sentul chickens with the genotype (++) had higher body weight and body weight gain than those with the (+-) genotype
Growth Hormone Gene Diversity and Quantitative Characteristics of Thin-Tailed Sheep in Muara Tembesi District Batang Hari Regency Pratama, Bondan; Depison, Depison; Gushairiyanto, Gushairiyanto; Gunawan, Asep; Sumantri, Cece
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.13

Abstract

Research on thin-tailed sheep (TTS) via quantitative and molecular characterization is essential for obtaining primary data on genetic diversity. The quantitative description includes body weight, weight gain, and body measurements. Molecular characterization is performed directly on structural genes. One gene that affects livestock growth and metabolism is the growth hormone (GH) gene. The commonly used method is PCR-RFLP. The research was conducted in two stages. Field research was conducted to obtain quantitative characteristic data, including body weight, weight gain, and body measurements, as well as blood samples from 62 TTS (31 males and 31 females). Research in the laboratory includes DNA extraction, PCR amplification, and restriction with the Msp1 enzyme. Data analysis included a t test to calculate the differences in body weight, weight gain, and body size between male and female TTS. T2-hotelling was used to compare the morphometric body sizes of the male and female TTS simultaneously, and principal component analysis was used to determine the body shape and size characteristics of the male and female TTS. The results revealed that the male TTS mean body weight, weight gain, and body size were significantly (P<0.05) greater than those of the female TTS. The results of the molecular analysis of the GH gene with the MspI cutting enzyme revealed that it is monomorphic with the ++ genotype. Conclusion: Body weight, weight gain, and body size were greater in males with TTS than in females with TTS. The body size characteristics of male and female TTS patients were body length, shoulder height, and chest circumference. The body shape characteristic was chest depth. The restriction of the GH|Msp1 TTS gene DNA fragment in exon 5 in the Muara Tembesi district is monomorphic.
Association of Growth Hormone Genes with Performance of Crossbreeds of Sentul Chicken and Arab Chicken Using PCR-RFLP Sinaga, Josua; Depison, Depison; Gushairiyanto, Gushairiyanto; Harahap, Ratna Sholatia
Jurnal Agripet Vol 25, No 2 (2025): Volume 25, No. 2 October 2025
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v25i2.42764

Abstract

This study evaluated the body weight, weight gain, body measurements, and genetic diversity of the Growth Hormone (GH) gene in male and female Sentul-Arab (SeA) crossbred chickens. It also explored the association between the GH gene and these traits. The research involved 30 male and 30 female SeA chickens reared from day-old chicks (DOC) to four months of age. The experimental method used direct observation to collect data, including body weight, weight gain, body measurements, and blood samples. Data were analyzed using t-tests, the Hotellings T test, and Principal Component Analysis (PCA). Molecular analyses assessed genotype and allele frequencies, Hardy-Weinberg equilibrium, heterozygosity levels, and Polymorphic Information Content (PIC). The results indicated that male SeA chickens had significantly higher (P0.05) body weight, weight gain, and body measurements compared to females. GH gene analysis revealed polymorphism, with genotype frequencies of +/+ (0.43), +/- (0.35), and -/- (0.22), consistent with Hardy-Weinberg equilibrium. The heterozygosity levels indicated moderate genetic diversity, while the PIC value fell within the low category. In conclusion, male SeA chickens exhibited superior body weight, weight gain, and body measurements compared to females, with chest circumference and shank length being critical indicators of body size. The polymorphic GH|TaqI gene was associated with body weight, weight gain, and body measurements, with the +/+ genotype showing the most favorable effects.
Co-Authors A. S. Wilastra Arfiana, BSM Asa Bela Sri Reformasi Nala Putri Asa Bela Sri Reformasi Nala Putri Asep Gunawan Cece Sumantri Depison - Depison Depison Depison Dika Irmaya Endri Musnandar Fauzan Ramadan Fetty Febriana Rahayu H. Ediyanto H. Suryani Helmi Ediyanto L. H. M. Gultom Lisna Lisna, Lisna M. Hari Setia Budi M., SAMIUN Muhammad Abdu Muhammad, Mus'ab Muthalib, Muthalib Nabilah Ika Puteri Pratama, Bondan Pratama, Shera Aditia Putri, Asa Bela Sri Reformasi Nala R. A. Muthalib Ratna Sholatia Harahap S. Erina Silvia Erina Sinaga, Josua Sofi Crisdayanti Sumadja, Wiwaha A Syafwan Syafwan Syafwan Syafwan Utama, Millisani W. P. B. Putra Widya Pintaka Bayu Putra Y. Alwi Zafitra A.