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All Journal Majalah Obstetri dan Ginekologi Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Jurnal Kedokteran Mulawarman Indonesian Andrology and Biomedical Journal Folia Medica Indonesiana
RINA YUDIWATI BM, RINA
Department Of Medical Biology, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Human Sperm Motility, Viability, and Morphology Decreased after Cryopreservation Darsini, Ninik; Hamidah, Berliana; Suyono, Seso Sulijaya; Ashari, Faisal Yusuf; Aswin, R Haryanto; Yudiwati, Rina
Folia Medica Indonesiana Vol. 55, No. 3
Publisher : Folia Medica Indonesiana

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The aim of this study was to analyze human sperm motility, viability, and morphology before and after cryopreservation. This true laboratory experimental study had pre and post randomized one group design. The study was conducted at the Embryology, Andrology, and Genetics Laboratory, Department of Medical Biology, Faculty of Medicine, Universitas Airlangga from August to November 2017. The eighteen samples of fresh semen were collected from male volunteers who agreed and signed the informed consent of the study. Samples were analyzed their motility, viability, and morphology before and after cryopreservation. Results of this study indicated differentiation between motility before and after cryopreservation. Cryopreservation process decreased progressive motility (42.22 + 9.46%; 17.83 + 6.24%; p< 0.0001) and increased the number of immotile spermatozoa (35.44 + 10.15%; 60.11 + 12.53%; p< 0.0001). Cryopreservation also decreased human sperm viability (73.78 + 8.91%; 40.83 + 12.89%; p< 0.0001) and morphology (10.94 + 4.96%; 7.39 + 3.90%; p< 0.0001). Cryopreservation of human spermatozoa caused the decreased of motility, viability, and morphology.
In vitro fertility test of human spermatozoa membrane protein fertilin beta antibody in mice (mus musculus balb/c) as immunocontraceptive candidate I'tishom, Reny; Soebadi, Doddy M; Hinting, Aucky; Lunardhi, Hamdani; Yudiwati, Rina
Folia Medica Indonesiana Vol. 52, No. 3
Publisher : Folia Medica Indonesiana

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One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.
Co-Authors Agustinus Agustinus Ashon Sa'adi Ashon Sa’adi Ashon Sa’adi Aswin, R Haryanto Aswin, R. Haryanto Aucky Hinting Bambang Poernomo Bambang Purwanto Bella Amanda Bella Amanda Berliana Hamidah Berliana Hamidah Darsini, Ninik Doddy M Soebadi Faisal Yusuf Ashari Hamdani Lunardhi Hamidah, Berliana I'tishom, Reny Lilik Djuari made sukma putra Nadhif Rashesa Brahmana Ninik Darsini Nurbayatin, Anita Pakpahan, Cennikon Pradana, E Pramesti, MPBD Relly Yanuari Primariawan, Relly Yanuari Rezia Octarina Seso Sulijaya Suyono Sulistiawati Sulistiawati Suyono, Seso Sulijaya Tanojo, Tjahjo Djojo Tirthaningsih, Ni Wajan Widjiati Widjiati, Widjiati Wigati, Kristanti Wanito Yohanes Widyakusuma Eka Saputra