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All Journal Journal of Biomedicine and Translational Research THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Medical Chemistry and Bioinformatics Indonesian Journal of Biomedicine and Clinical Sciences
Sri Suciati Ningsih
Jurusan Biologi, Universitas Negeri Padang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics

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Journal : Indonesian Journal of Medical Chemistry and Bioinformatics

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Immunocytochemistry Evaluation of Fibroblast Response to Garlic (Allium sativum) Extract Targeting MMP9: In Vitro and In Silico Approaches Ningsih, Sri Suciati; Zuraidah, Endah; Handayani, Supri Irianti; Ardiansyah, Ahmad Ali Irfan; Wahyuni, Ningrum
Indonesian Journal of Medical Chemistry and Bioinformatics
Publisher : UI Scholars Hub

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Abstract

Background: Fibrosis involves excessive extracellular matrix (ECM) deposition, with matrix metalloproteinase-9 (MMP-9) playing a critical role. Natural compounds targeting MMP-9 may offer therapeutic potential. Objective: This study evaluated the antifibrotic effect of Allium sativum extract on fibroblast cells through in vitro and in silico approaches targeting MMP-9. Methods: Human dermal fibroblasts were treated with A. sativum extract (25, 50, and 100 µg/mL) for 24 and 48 hours. Cell viability was assessed using MTT assay. MMP-9 expression was analyzed via quantitative real-time PCR (qRT-PCR) and immunocytochemistry (ICC). Major garlic compounds were docked to MMP-9 using AutoDock Vina. Results: The 50 µg/mL concentration maintained >85% cell viability and significantly reduced MMP-9 gene and protein expression. S-allyl cysteine exhibited the strongest binding affinity (−6.8 kcal/mol) and formed hydrogen bonds with key residues (Glu402, Ala189, His405) of MMP-9. Conclusion: Allium sativum extract demonstrates antifibrotic activity by downregulating MMP-9 expression and inhibiting its function, supporting its potential as a natural MMP-9 inhibitor for fibrosis-related disorders.
Co-Authors Ahmad Ali Irfan Ardiansyah, Ahmad Ali Irfan Ardiansah, Fery Avissa, Rizkyana Budiman, Muhamad Arif ERLIN LISTIYANINGSIH Lestari, Dwi Retna Permatasari, Shinta Dewi Sanjaya, Arief Indra Sari, Dewi Hambar Sari, Shinta Dewi Permata Septiasari, Alfina Stujanna, Endin Nokik Sukarya, Wawang S. Supri Irianti Handayani Suryadi Islami Ujianti, Irena Wahyuni, Ningrum Zuraidah, Endah