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All Journal SAINS DAN MATEMATIKA INDONESIAN JOURNAL OF PHARMACY Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Journal of Fisheries & Marine
Muhammad Nursid
Balai Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Kementrian Kelautan dan Perikanan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology

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BIOACTIVE TERPENOID FROM THE BALINESE NUDIBRANCH Hypselodoris infucata Mudianta, I Wayan; Martiningsih, Ni Wayan; Prasetia, I Nyoman Dodik; Nursid, Muhammad
Indonesian Journal of Pharmacy Vol 27 No 2, 2016
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (806.52 KB) | DOI: 10.14499/indonesianjpharm27iss2pp104

Abstract

Marine organisms, in particular nudibranchs (Mollusca: nudibranchia), are known as a rich source of chemically diverse secondary metabolites exhibiting potential as antimalarial, anti-inflammatory, antiviral and anticancer activity. We presented the chemical investigation of an extract of nudibranch Hypselodoris infucata collected from Bali, an unexplored water but rich in nudibranch diversity. The extract contained the known (–)-furodysinin (1), a furanosesquiterpene that for the first time isolated from this species. Metabolite 1 was purified by chromatography and the structure was characterised by comparison of 1H NMR data with that of the reported data. The absolute configuration was determined by comparing the optical rotation values with the known enantiomer. In vitro cytotoxic activity of compound 1 against HeLa cell line displayed an IC50 at 102.7µg/mL. We also report for the first time the development of a method to assay nudibranch extracts for their feeding deterrence activity against local shrimps Penaeus vannamei. The extract show food rejection with highly significant difference in respect to the control (P = 0.0061) at natural concentration of 3.0mg/mL.Keywords: nudibranch, natural product, feeding deterrence, Hypselodoris
Kadar Emestrin Emericella nidulans yang Dikultivasi pada Media dan Waktu yang Berbeda Nursid, Muhammad; Fajarningsih, Nurrahmi Dewi; Marraskuranto, Endar; Chasanah, Ekowati
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 2 (2012): Desember 2012
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v7i2.71

Abstract

Penelitian untuk mengetahui pengaruh jenis media dan waktu kultivasi terhadap kadar senyawa emestrin dari kapang Emericella nidulans telah dilakukan. Kapang dikultivasi dalam 2 jenis media yaitu malt extract broth (MEB) dan soluble starch-water-soytone (SWS) dalam waktu 1, 2, 3, 4, dan 5 minggu. Kadar emestrin dianalisis dengan menggunakan kromatografi cair kinerja tinggi (KCKT). Hasil penelitian menunjukkan bahwa jumlah biomassa dan kadar emestrin tertinggi dihasilkan E.nidulans yang dikultivasi dalam media SWS selama 5 minggu.
Bioprospeksi Spons, Karang Lunak dan Ascidian Asal Taman Nasional Laut Kepulauan Wakatobi: Antitumor dan Antioksidan Fajarningsih, Nurrahmi Dewi; Nursid, Muhammad; Januar, Hedi Indra; Wikanta, Thamrin
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): Desember 2013
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v8i2.60

Abstract

Penelitian ini bertujuan untuk mengetahui potensi manfaat (bioprospeksi) spons, karanglunak, dan ascidian asal Taman Nasional Laut (TNL) Kepulauan Wakatobi sebagai antitumor dan antioksidan. Sampel makroinvertebrata laut diambil dengan menggunakan scuba diving di empat stasiun pengambilan di perairan TNL Kep. Wakatobi. Sebanyak 74 sampel diuji aktivitas antitumornya terhadap 3 jenis sel lestari tumor (HeLa, MCF7, SKOV3) dengan metode Thiazolyl Blue Tetrazolium Blue (MTT) dan diuji pula aktivitas antioksidannya dengan menggunakan metode DPPH (2,2-diphenyl pikryl hidrazil). Sampel yang dapat menghambat >50% sel lestari tumor pada konsentrasi 20 µg/ml dikategorikan sebagai sampel aktif antitumor. Sampel yang memiliki aktivitas penghambatan radikal bebas >50% pada konsentrasi 100 µg/ml dalam uji DPPH dikategorikan sebagai sampel yang aktif antioksidan. Hasil penelitian menunjukkan bahwa terdapat 3 sampel (ascidian W0208; spons W2408 dan W2508) yang aktif terhadap tiga jenis sel lestari tumor, 11 sampel aktif terhadap dua jenis sel lestari tumor dan 22 sampel aktif terhadap satu jenis sel lestari tumor. Dari 74 sampel yang diuji aktivitas antioksidannya, terdapat 8 sampel yang tergolong aktif (7 sampel spons dan 1 sampel karang lunak).
Uji Aktivitas Antioksidan, Toksisitas, The Assays of The Antioxidative Activity, Toxicity, and Cytotoxicity of The Red Algae. Wikanta, Thamrin; Januar, Hedi Indra; Nursid, Muhammad
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 4 (2005): JPPI Ed Pascapanen
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v11i4.475

Abstract

Riset tentang aktivitas antioksidan, toksisitas dan sitotoksisitas ekstrak Rhodymenia palmata telah dilaksanakan. Uji aktivitas antioksidan dilakukan dengan metode DPPH (1,1‑ difenil‑2­pikrilhidrazil).Uji toksisitas terhadap Artemia salina dilakukan dengan metode BSLT (Brine Shrimp Lethality Test). Uji sitotoksisitas terhadap sel lestari tumor HeLa ditentukan dengan metode MTT (3‑(4,5‑dimetilthiazol‑2yi)‑2,5‑difeniI tetrazolium bromida). Hasil uji menunjukkan bahwa ekstralk kasar metanol memiliki aktivitas antioksidan rendah (IC .0~464,89 ppm). Fraksi etil asetat memiliki toksisitas paling tinggi (L C50 =5,36 ppm), dan fraksi n‑heksana memiliki toksisitas rendah (LC 0=55,20 ppm), sedangkan fraksi metanol termasuk kelompok tidak toksik (LC 50=39012,95 ppm). Uji sitotoksisitas terhadap sel lestari tumor HeLa menunjukkan bahwa fraksi etil asetat memiliki sitotolksisitas rendah (LC 5,=432,43 ppm), sedangkan fraksi n‑heksana memiliki sitotoksisitas tinggi (LC 5,=29,68 ppm).
ANALISIS KUANTITATIF B-KAROTEN DAN UJI AKTTVITAS KAROTENOID DALAM ALGA COKLAT TURBINARIA DECURRENS Biranti, Fransisca; Nursid, Muhammad; Cahyono, Bambang
JURNAL SAINS DAN MATEMATIKA Volume 17 Issue 2 Year 2009
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6725.173 KB)

Abstract

ABSTRACT---One of the Indonesian marine natural resources, brown alga of Turbinaria decurrens, used in pharmacy in order of carotenoid pigment as antioxidant. We interested in analysis B-caroten in Turbinaria decurrens and antioxidant and anti-tumor activity. The method that is use to divide carotenoid is the hierarchy maseration method using n-hexane, ethyl acetate  and methanol  and  then using chromatography column. To analyze the qualitative carotenoid uses HPLC  by Crc column and eluenmethanol-acetonitrile  (3:I).  Meanwhile,  the test of bioactivity carotene  uses  radical DPPH  (1I'difenil-2-pibilhidrozil)  to  test  of  antioxidant  qnd  sitotoksik asscy with  MTT  [3,(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrcaolium  bromideJ  for  HeLa tumor cells  to test of anti-tumor. The  result of this research  is  B -caroten  thqt is in qctract  has 0.00387%.  Moreover,  bioacttvity  test  shows  that B -caroten  fraction does  notactive  to neutralize  of DPPHfree radical than  ascorbic  aci{  but  it shows  the  acttvity  to kill HeLa  tumor  cells.Keywords  : Turblnaria decunens,  Carolenoid,  Antioksidan,  Antitumor
Effects of Sun and Oven Drying on The Physicochemical Composition of Indonesian Sandfish (Holothuria scabra) Fitri, Dian Arsita; Purwanti, Nanik; Nursid, Muhammad; Patantis, Gintung
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 1 (2024): May 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.886

Abstract

Sandfish (Holothuria scabra) is a type of sea cucumber with high economic value. Sandfish are potentially used as food, pharmaceutical, and cosmetic ingredients. This research aimed to determine the effects of sun and oven drying on the physicochemical composition of sandfish collected from Pesawaran Waters, Lampung, Indonesia. The physicochemical composition includes the proximate composition, saponin content, collagen content, and metabolite profiles. The sea cucumbers were divided into two groups; the first group was boiled for 30 minutes, whereas the second group was unboiled. The sea cucumbers were oven-dried (temperature 60 °C for 24 h) and sun-dried (3 days from 08:00 a.m. until 5:00 p.m.). Fresh sandfish (without drying) were used as control samples. The dried and fresh sandfish were analyzed for their proximate composition (moisture, ash, fat, protein, and carbohydrates) and collagen content. The dried and fresh sandfish were extracted by maceration using ethanol. The extract was analyzed for its saponin content and metabolite profiles. The results showed no significant differences (p0.05) between oven-dried and sun-dried sandfish regarding their proximate composition and collagen content. However, the saponin content in boiled sandfish extract was significantly different (p0.05) from the saponin content in sandfish extract that was unboiled. The highest saponin content of all treatments was in the boiling and oven-drying treatment (N1P2), i.e., 7.418 mg quillaja/10mg extract. The Fourier Transform Infrared (FTIR) profile indicated that the functional groups in the sandfish extract did not change during the drying process. The findings indicate that oven and sun drying do not negatively affect the physicochemical composition of the sandfish, especially its bioactive compounds, i.e., saponin and collagen. 
Screening of Antitumor Bioactivity of Fungi Associated with Macro Algae and Sponge from Indrayanti Beach, Jogjakarta Fajarningsih, Nurrahmi Dewi; Nursid, Muhammad; Chasanah, Ekowati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.25

Abstract

This research was aimed to isolate marine derived-fungi which associated with macro algae and sponge from Indrayanti Beach, Jogjakarta and to screen the antitumor (T47D and HeLa) bioactivity of the fungi extracts. Three solid media of MEA (malt extract agar), GPY (glucose peptone yeast) and MFM (minimal fungi medium) were used as isolation medium. Each of the pure fungi isolates was then cultivated in 100 ml of liquid medium for 4 weeks at room temperature (27-28°C) in static conditions. The antitumor activity of the fungi extracts were tested against breast tumor cells (T47D) and cervical cancer cells (HeLa) using Thiazolyl Blue Tetrazolium Blue (MTT) assay method. A number of 21 isolates of fungi were isolated from 4 macro algae and 1 sponge samples. The identification of fungi isolate was conducted using combination of molecular approach (ITS1-5.8S-ITS4 DNA regions) and macro-micro morphological characteristics. Among those 21 marine fungi species isolated, MFGK-21 extract showed the best anti-servical tumor (HeLa) with an IC50 value of 240.1 µg/ml and MFGK-27 extract showed the best anti-breast tumor (T47D) with an IC50 value of 59.6 µg/ml. The MFGK-21 fungi isolate was identified as Penicillium steckii, while the MFGK-27 fungi isolate was identified as Aspergillus sydowii.
Tyrosinase Inhibitory Activity of Methanolic Extract and Fractions from Green Seaweed Ulva lactuca Gazali, Mohamad; Maknun, Fazidatul Lu’luil; Husni, Amir; Nurjanah, Nurjanah; Nursid, Muhammad; Hasanah, Uswatun; Zuriat, Zuriat; Syafitri, Rina
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 20, No 1 (2025): May 2025
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.915

Abstract

Ulva lactuca, a widely available green seaweed, has demonstrated promising bioactivity as a tyrosinase inhibitor. This study aimed to evaluate the tyrosinase inhibitory activity of U. lactuca extracts and identify the active compounds responsible. The seaweed was extracted using methanol via maceration, followed by partitioning with hexane, chloroform, and water. Total phenolic and flavonoid contents were determined using UV-VIS spectrophotometry, and tyrosinase inhibition was assessed in vitro. Among the tested fractions, the hexane fraction exhibited the highest tyrosinase inhibitory activity with an IC50 value of 584.34 ± 35.07 µg/mL, classifying it as an active inhibitor. Although this activity was weaker than kojic acid (IC50 = 11.07 ± 0.86 µg/mL), the hexane fraction showed relatively high phenolic (11.66 ± 0.53 mg GAE/g) and flavonoid (9.75 ± 0.30 mg QE/g) contents. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified several bioactive compounds, notably 2,4-dimethoxycinnamic acid, chalcone, and derivatives such as 2,6-dihydroxybenzoic acid and phloretin. These findings highlight the potential of U. lactuca, particularly its hexane fraction, as a source of natural tyrosinase inhibitors. This opens avenues for its application in the pharmaceutical and cosmetic industries, especially in formulations targeting hyperpigmentation and skin brightening.
In silico and In vitro Antibacterial Activity of Centella asiatica Leaves Bioactive Compounds Against Aquaculture Pathogenic Bacteria Andriyanto, Septyan; Maftuch, Maftuch; Andayani, Sri; Nafiqoh, Nunak; Gardenia, Lila; Novita, Hessy; Nursid, Muhammad
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.72072

Abstract

Graphical Abstract     Highlight Research The GC-MS analysis of the asiatica leaves extracts identified 53 bioactive compounds. The crude extracts of asiatica showed antibacterial efficacy against fish pathogenic bacteria. 13-Hexyloxacyclotridec-10-en-2-one has the potential to be an inhibitor of DNA gyrase. Bioactive compounds derived from C. asiatica leaves extracts show potential as antibacterial agents.     Abstract Antimicrobial agents are crucial for managing bacterial infections in fish cultures. Centella asiatica is a medicinal plant recognised for its diverse bioactive compounds with important antibacterial properties. The present study aimed to investigate the antibacterial activity of C. asiatica leaves bioactive compounds on fish pathogenic bacteria using an in vitro and in silico approach. The maceration method was used to extract bioactive compounds from C. asiatica leaves and was identified using Gas Chromatography-Mass Spectrometry (GC-MS). In vitro analysis of antibacterial activity was evaluated using the minimum inhibitory concentration method. While in silico molecular docking is applied alongside assessing Lipinski's rules of five, as well as absorption, distribution, metabolism, excretion, and toxicity properties. The result of the GC-MS examination of the C. asiatica leaf extracts identified 53 bioactive compounds. In vitro studies showed antibacterial efficacy of leaf extracts against fish pathogenic bacteria (Streptococcus agalactiae, Bacillus subtilis, and Staphylococcus aureus) with minimum inhibitory concentration values of 12,5 mg/ml. In silico molecular docking analysis showed that several bioactive compounds have the potential to be DNA gyrase inhibitors. Compound 13-Hexyloxacyclotridec-10-en-2-one has the highest inhibition with binding energy of −7,4 Kcal/mol compared to ciprofloxacin as drug standard with a binding energy value −7,3 Kcal/mol. The following compound is gamma.-Muurolene (−6,7 Kcal/mol), Copaene (−6,6 Kcal/mol) and Humulene (−6,6 Kcal/mol). These results suggest that bioactive compounds of C. asiatica leaves extracts hold promise as potential antibacterial agents for treating fish pathogenic bacteria infections.
Comparative Study of Pre-Extraction Treatments and Buffer Modifications for Macroalgal Lectin Extraction Fajarningsih, Nurrahmi Dewi; Nursid, Muhammad; Nugroho, Hartanto; Nuringtyas, Tri Rini; Isnansetyo, Alim
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 3 (2023): December 2023
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.852

Abstract

Getting an efficient extraction approach is a crucial step in bioactive protein research, particularly lectin. This research aimed to examine the efficiency of cryogenic-grinding (CG) and freeze-dried-grinding (FG) pre-extraction treatments, and also the incorporation of phenylmethylsulphonyl fluoride (PMSF), Tween 80, polyvinylpolypyrrolidone (PVPP), 70% Ethanol (EtOH), or combination of the chemicals in the 20 mM phosphate buffered saline pH 7 (PBS) for extracting lectin from Ulva lactuca, Sargassum polycystum, and Hydropuntia edulis. The lectin content of the extracts was determined using the hemagglutination activity (HA) assay. The phenolic content was measured to determine its impact on the lectins’ HA. Lectin extraction efficiency was determined by analyzing the extracts’ minimum agglutination concentration (MAC) and total hemagglutination activity (THA). CG pre-extraction treatment produced slightly higher THA than FG, making it slightly more efficient. The EtOH treatment efficiently extracted lectin from U. lactuca and H. edulis by substantially reducing the polyphenol (PPs) content, lowering the MAC, and increasing the THA. The EtOH treatment significantly decreased the PPs and HA of the S. polycystum, suggesting that the HA is most likely produced by the PPs rather than the lectin content. Tween 80 raised the THA of U. lactuca by 17-fold with native rabbit erythrocyte compared to the control but did not affect the THA of H. edulis and S. polycystum. Several different effects of chemicals incorporated in the extraction buffers suggested that the optimum macroalgal lectin extraction strategy is species-dependent.
Co-Authors ALIM ISNANSETYO Amir Husni Andriyanto, Septyan Bambang Cahyono Ekowati Chasanah ENDAR MARRASKURANTO Fitri, Dian Arsita Fransisca Biranti Gintung Patantis HEDI INDRA JANUAR I Nyoman Dodik Prasetia I Wayan Mudianta Lila Gardenia Maftuch Maftuch Maknun, Fazidatul Lu’luil Mohamad Gazali N. Nurjanah Nafiqoh, Nunak NANIK PURWANTI Ni Wayan Martiningsih Novita, Hessy Nugroho, Hartanto Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Rina Syafitri, Rina Sri Andayani Thamrin Wikanta Tri Rini Nuringtyas Uswatun Hasanah Zuriat, Zuriat