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Journal : Jurnal Agro Wiralodra

KEEFEKTIFAN BAKTERI Serratia ENDOSIMBION WBC TERHADAP ULAT GRAYAK (Spodoptera litura F.) DI LABORATORIUM ENTOMOLOGI BBPOPT JATISARI KARAWANG Dwimartina, Fina; Rostaman, Rostaman; Soesanto, Loekas
Jurnal Agro Wiralodra Vol 3 No 1 (2020): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v3i1.39

Abstract

Penelitian ini bertujuan untuk: 1) Mengetahui pengaruh perlakuan bakteri Serratia Endosimbion WBC terhadap mortalitas serangga S. litura; 2) Mengetahui pengaruh pemberian bakteri tersebut terhadap daya konsumsi larva S. litura; 3) Mengetahui pengaruh bakteri tersebut terhadap perkembangan serangga. Penelitian dilaksanakan di Laboratorium Entomologi BBPOPT Jatisari Karawang, pada bulan Maret sampai Juli 2014. Penelitian ini dilakukan dengan metode eksperimen, menggunakan rancangan acak terpisah yang terdiri dari 10 perlakuan dan 2 kali ulangan. Variabel yang diamati adalah mortalitas (%), tingkat konsumsi larva, dan perkembangan larva. Hasil penelitian menunjukkan bakteri Serratia tidak menimbulkan kematian pada serangga uji (mortalitas 0%), tidak berbeda nyata pada daya konsumsi larva, dan tidak berpengaruh nyata terhadap persentasi larva yang menjadi pupa. Bakteri Serratia berpengaruh nyata terhadap berat pupa dan persentasi pupa menjadi imago.
KARAKTERISTIK MORFOLOGI DAN FISIOLOGI BAKTERI ENDOFIT DAN RIZOBAKTERI DARI TANAMAN CENGKEH SEHAT dwimartina, fina; Joko, Tri; Arwiyanto, Triwidodo
Jurnal Agro Wiralodra Vol 4 No 1 (2021): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agro wiralodra.v4i1.58

Abstract

One of the determining factors in disease control is the use of superior seeds which have good quality and quantity. The use of biological control agents that form a symbiotic mutualism in their host plants can be used as an effort to obtain quality clove seeds. Endophytic bacteria and rhizobacteria have been reported as potential biological control agents for many plant diseases because of their safety in humans and non-target organisms. The purpose of this study was to identify the physiological and morphological characteristics of endophytic and rhizobacterial isolates obtained from clove plants. A total of 46 endophytic bacterial isolates were isolated from healthy clove roots, stems, leaves and flowers. Also, 66 isolates were isolated from the soil around the rhizosphere area. Based on the results of the identification of physiological and morphological characteristics, it is evident that the endophytic and rhizobacterial bacteria tested have the same character as Bacillus spp.
KEEFEKTIFAN BAKTERI Serratia ENDOSIMBION WBC TERHADAP ULAT GRAYAK (Spodoptera litura F.) DI LABORATORIUM ENTOMOLOGI BBPOPT JATISARI KARAWANG Fina Dwimartina; Rostaman Rostaman; Loekas Soesanto
Agro Wiralodra Vol. 3 No. 1 (2020): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v3i1.39

Abstract

Penelitian ini bertujuan untuk: 1) Mengetahui pengaruh perlakuan bakteri Serratia Endosimbion WBC terhadap mortalitas serangga S. litura; 2) Mengetahui pengaruh pemberian bakteri tersebut terhadap daya konsumsi larva S. litura; 3) Mengetahui pengaruh bakteri tersebut terhadap perkembangan serangga. Penelitian dilaksanakan di Laboratorium Entomologi BBPOPT Jatisari Karawang, pada bulan Maret sampai Juli 2014. Penelitian ini dilakukan dengan metode eksperimen, menggunakan rancangan acak terpisah yang terdiri dari 10 perlakuan dan 2 kali ulangan. Variabel yang diamati adalah mortalitas (%), tingkat konsumsi larva, dan perkembangan larva. Hasil penelitian menunjukkan bakteri Serratia tidak menimbulkan kematian pada serangga uji (mortalitas 0%), tidak berbeda nyata pada daya konsumsi larva, dan tidak berpengaruh nyata terhadap persentasi larva yang menjadi pupa. Bakteri Serratia berpengaruh nyata terhadap berat pupa dan persentasi pupa menjadi imago.
Karakteristik Morfologi Dan Fisiologi Bakteri Endofit Dan Rizobakteri Dari Tanaman Cengkeh Sehat Fina Dwimartina; Tri Joko; Triwidodo Arwiyanto
Jurnal Agro Wiralodra Vol. 4 No. 1 (2021): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v4i1.58

Abstract

One of the determining factors in disease control is the use of superior seeds which have good quality and quantity. The use of biological control agents that form a symbiotic mutualism in their host plants can be used as an effort to obtain quality clove seeds. Endophytic bacteria and rhizobacteria have been reported as potential biological control agents for many plant diseases because of their safety in humans and non-target organisms. The purpose of this study was to identify the physiological and morphological characteristics of endophytic and rhizobacterial isolates obtained from clove plants. A total of 46 endophytic bacterial isolates were isolated from healthy clove roots, stems, leaves and flowers. Also, 66 isolates were isolated from the soil around the rhizosphere area. Based on the results of the identification of physiological and morphological characteristics, it is evident that the endophytic and rhizobacterial bacteria tested have the same character as Bacillus spp.
Analisis Molekuler Burkholderia glumae Pada Varietas Padi Ciherang Di Sawah Tadah Hujan Lingkungan Universitas Wiralodra Indramayu Dwimartina, Fina; Laila, Fadhillah
Jurnal Agro Wiralodra Vol. 5 No. 1 (2022): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v5i1.65

Abstract

Deteksi patogen penyebab penyakit tumbuhan dapat dilakukan baik secara langsung maupun tidak langsung. Deteksi secara langsung umumnya dilakukan dengan menggunakan metode serologi dan molekuler. Deteksi dan identifikasi bakteri patogen dengan teknik molekuler dapat dilakukan dengan teknik polymerase chain reaction (PCR). Penyakit hawar malai yang disebabkan oleh Burkholderia glumae dilaporkan menginfeksi tanaman padi di Indonesia. B. glumae dapat terbawa benih sehingga berpotensi menyebar dengan cepat. Penelitian ini bertujuan untuk mengetahui wilayah sebar penyakit hawar malai di sawah tadah hujan lingkungan Universitas Wiralodra Indramayu. Metode yang dilakukan meliputi pengambilan sampel biji padi di sawah tadah hujan lingkungan Universitas Wiralodra Indramayu. Biji padi secara acak diambil langsung dari sawah tadah hujan serta dicatat varietas serta nama daerah pengambilan sampel, kemudian diuji secara molekuler. Hasil amplifikasi PCR terhadap 3 sampel biji padi menunjukkan ketiga sampel tersebut negatif terinfeksi B. glumae. Sampel biji padi yang negatif terdeteksi B. glumae dapat terjadi karena pada saat pengambilan sampel, wilayah Indramayu sedang memasuki musim penghujan, sehingga potensi berkembangnya penyakit hawar malai akan menurun.
Respon Pertumbuhan Dan Hasil Bawang Merah (Allium Ascalonicum L.) Terhadap Inokulasi Fungi Mikoriza Arbuskula (FMA) Dan Pupuk Limbah Baglog Asad, Faisal Al; Dwimartina, Fina; Laila, Fadhillah
Jurnal Agro Wiralodra Vol. 6 No. 1 (2023): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v6i1.87

Abstract

Shallots are one of Indonesia's leading horticultural commodities. Domestic demand for shallots for this commodity is very high in line with population growth. On the other hand, shallot cultivation is generally carried out with high production inputs such as the unwise use of inorganic fertilizers. This will have an impact on the decline in soil quality and will ultimately reduce production. One way that can be done to overcome this is the use of organic fertilizers such as arbuscular mycorrhizal fungi (FMA) and baglog waste fertilizer. The experimental design used in this study was a two-factor Randomized Block Design (RAK). The first factor is the dose of AMF, which consists of: M0 = 0 gr, M1 = 5 gr, and M2 = 10 gr. The second factor is the dose of baglog waste fertilizer, which consists of: B0 = 0 tons/ha, B1 = 5 tons/ha, and B2 = 10 tons/ha. The results showed: inoculation of AMF and baglog waste fertilizer had not been able to increase the growth of shallot plants, inoculation of 10 g of AMF could increase the number of tubers/clump by 21.53% compared to control, and the combination of treatment without AMF and without baglog waste fertilizer resulted in fresh weight of tubers/clump. the best clump, which is 106.68 gr.
PERFORMA AGRONOMI BEBERAPA GALUR HARAPAN TANAMAN PADI (Oryza sativa L.) PADA LAHAN SAWAH TADAH HUJAN DI DESA KENDAYAKAN KECAMATAN TERISI Ofdiansyah, Rizky; Sumarna, Pandu; Tohidin, Tohidin; Mahmud, Yudhi; Dwimartina, Fina
Jurnal Agro Wiralodra Vol. 6 No. 2 (2023): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v6i2.99

Abstract

Produktivitas padi pada lahan sawah tadah hujan umumnya masih rendah, karena terbatasnya air untuk kebutuhan pertumbuhan tanaman. Varietas Unggul Baru (VUB) merupakan salah satu inovasi teknologi yang dapat diandalkan untuk meningkatkan produktivitas padi. Tujuan penelitian ini adalah mengetahui perbedaan performa pertumbuhan dan hasil beberapa galur harapan tanaman padi (Oryza sativa L.) pada lahan sawah tadah hujan. Penelitian ini dilaksanakan di Desa Kendayakan Kecamatan Terisi Kabupaten Indramayu, pada Bulan Desember sampai dengan April 2021-2022. Metode penelitian yang digunakan adalah metode eksperimen dengan menggunakan Rancangan Acak Kelompok (RAK) dengan 6 perlakuan. Adapun enam perlakuan itu adalah V1 = TCIPB202101, V2 = TCIPB202103, V3 = TCIPB202105, V4 = TCIPB202105, V5 = Inpari30, V6 = Ciherang. Masing-masing perlakuan diulang sebanyak 4 kali. Dengan demikian terdapat 24 unit percobaan. Hasil penelitian menunjukan Galur TCIPB202105 menghasilkan gabah kering giling tertinggi, yaitu 12 kg/petak atau setara 11,92 ton/ha dan untuk varietas pembanding Inpari 30 menghasilkan 9 kg/petak atau setara 9,57 ton/ha dan untuk Ciherang menghasilkan 10 kg/petak atau setara 10,52 ton/ha.
Perbanyakan Fungi Mikoriza Arbuskula (FMA) Indigenous Pohon Mangga Lokal Indramayu Dari Berbagai Sumber Dan Dosis Inokulum Pada Tanaman Inang Pueraria javanica Rusminah, Putri Shinty Charisma Yuyun; Sumarna, Pandu; Asad, Faisal Al; Laila, Fadhillah; Dwimartina, Fina
Jurnal Agro Wiralodra Vol. 7 No. 2 (2024): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v7i2.111

Abstract

Mango fruit (Mangifera indica L.) is one of the fruits that is popular with Indonesian people because it contains quite complete vitamins and is affordable. Development of mango cultivation in Indramayu is carried out throughout the year, but mango production fluctuates at each harvest time. One of the reasons for this is alternate bearing, namely the uncertain condition of the mango harvest every year due to internal or climatic factors. One effort that can be made to control this phenomenon is by using biological fertilizers such as Arbuscular Mycorrhizal Fungi (FMA). The host plant used for AMF propagation is Pueraria javanica because it is more resistant to humidity and low temperatures compared to sorghum. This research aims to obtain a source of AMF inoculum that is suitable for vegetative growth of Pueraria javanica and the highest number of spores and to obtain the right dose for the development of AMF and growth of the Pueraria javanica host. This research used a factorial Randomized Block Design with 2 factors where the first factor was AMF Inoculum Source which consisted of three types ( Lohbener Village mango trees, Jatisawit Village mango trees, Krasak Village mango trees). The second factor is the dose of the AMF inoculum source which consists of three levels of spore number (60 g, 80 g, 100 g). The results of the study showed that there was no interaction between the inoculum source and the dose on the growth of the host plant Pueraria javanica. The inoculum source of mango trees in Krasak Village had the best influence on the number of leaves of the host plant Pueraria javanica.
Pengaruh Sumber Inokulum dan Dosis pada Perbanyakan Fungi Mikoriza Arbuskula (FMA) dengan Tanaman Inang Jagung Manis (Zea mays saccharate, sturt) Desvita, Desvita; Mahmud, Yudhi; Asad, Faisal Al; Dwimartina, Fina; Laila, Fadhillah
Jurnal Agro Wiralodra Vol. 7 No. 1 (2024): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v7i1.119

Abstract

Arbuscular Mycorrhizal Fungi (FMA) are a type of mycorrhiza that is obligative, symbiotic that requires a host plant. Sweet corn plants are suitable plants to be used as host plants because of their greedy nature for nutrients. This study aims to determine the effect between mycorrhiza and different doses of FMA on the vegetative growth of sweet corn host plants and the highest number of spores with inoculum sources derived from mango plants. The research was carried out in the greenhouse of the Faculty of Agriculture and Science Laboratory, Wiralodra University in March – August 2023. The experimental design used in this study was a two-factor Randomized Group Design. The first factor is the source of inoculum consisting of S 1 = Source of Inoculum from Biotroph, S 2 = Source of Inoculum from Lobener Village, S 3 = Source of Inoculum from Jatisawit Village and S 4 = Source of Inoculum from Krasak Village. The second factor is the dose of FMA, which consists of: D1 = 20 grams / Polybag, D2 = 40 grams / polybag. The results showed that there was no interaction between inoculum source and FMA dose on spore count, FMA colonization, plant height, root length, root volume, dry root weight, fresh header weight and dry header weight. But it has a noticeable effect on the number of leaves. The best source of inoculum and FMA dose is shown by the treatment of S1 source of inoculum from Biotrop with a combination dose of Biotrope 20 grams / polybag and Biotrope 40 grams / polybag.
Identifikasi Keanekaragaman dan Dominansi Gulma Pada Lahan Pertanaman Mangga Gedong Gincu di Desa Lohbener Indramayu Dwimartina, Fina; Asad, Faisal Al
Jurnal Agro Wiralodra Vol. 8 No. 1 (2025): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v8i1.138

Abstract

Mango plants can grow optimally if planted in a suitable environment, but will be hampered if there are factors inhibiting their growth. Weeds are plants that can limit or inhibit the growth of plants cultivated on a land (1). This is because weeds become competitors for the main plants in obtaining nutrients, water, light and growing space (2). Weeds can also host major plant pathogens and pests (3) and can produce allelochemical compounds, namely chemical compounds that can suppress the growth of cultivated plants through an allelopathic process (4). It is important to study weed diversity to determine the composition and structure of weeds in mango plantations and to determine appropriate control. Weed diversity is influenced, among other things, by environmental conditions (5). The aim of this research is to determine the diversity and dominance of weeds in mango plantations. This research uses a qualitative method by identifying sample weeds, as well as a quantitative method by analyzing weed vegetation. Sampling was carried out using the Quadratic method using plots measuring 0.5×0.5 m2 randomly by throwing the plots into the mango cultivation land 10 times so that there were 10 sample plots. The results of the research showed that in the Gedong Gincu mango plantations in Lohbener Indramayu Village, 2 groups of weeds were found, namely grass weeds consisting of 1 species, and broadleaf weeds consisting of 2 species. The dominant weeds on the land are Imperata cylindrica (SDR value 56.94%), and Ruellia tuberosa (SDR value 19.19%).