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All Journal Majalah Ilmiah Sultan Agung Medali Jurnal : Media Dental Intelektual ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dentino: Jurnal Kedokteran Gigi Padjadjaran Journal of Dentistry Odonto dental journal Jurnal Pengabdian Masyarakat Dentmas

Rahmawati Sri Praptiningsih

Faculty Of Dentistry Universitas Islam Sultan Agung

Author-ID : 344840

Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The Effectiveness of α-Mangostin in Reducing the Streptococcus Mutans Biofilm Thickness Rahmawati Sri Praptiningsih; Arlina Nurhapsari; Rifqi Wahyu Febrian
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 11, No 2 (2022): November
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v11i2.15809

Dental caries occurs due to the demineralization of tooth structure caused by microorganisms in colonies called biofilms. One of the microorganisms involved in dental caries is Streptococcus mutans. Oral mouthwash, in addition to mechanical cleaning, is known to prevent the growth of oral microorganisms. Mangosteen is known as an anti-cancer ingredient with high anti-bacterial properties. This study aims to identify the effectiveness of mangosteen skin extract (Garcinia mangostana L.) in decreasing Streptococcus mutans biofilm. The study is experimental research with a post-test control group design. The research sample was divided into five groups; 3 treatment groups and 2 control groups. The biofilm thickness test was carried out with OD (Optical density) with a wavelength of about 620 nm using an ELISA reader. Kruskal Wallis analysis was employed as a non-parametric statistical test analysis. Statistical Kruskal Wallis indicated significant differences in the thickness of 5 test groups of Streptococcus mutans. The lowest average yield of biofilm thickness was in the α-mangosteen group at 12.5 g/ml. Conclusion: α-mangosteen in mangosteen skin extract effectively reduced the thickness of Streptococcus mutans biofilm.

EFFECT OF THE PROBIOTIC STREPTOCOCCUS SANGUINIS ON THE FORMATION OF STREPTOCOCCUS MUTANS BIOFILM IN ARTIFICIAL SALIVA Rahmawati Sri Praptiningsih; Sheilia Siwi Pranantri; Anggun Feranisa
Dentino : Jurnal Kedokteran Gigi Vol 7, No 2 (2022)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v7i2.14616

Background: Dental caries is a disease of the hard tissues of the teeth that most often occurs with a prevalence rate of about 45.3%. Dental caries is occuring because of the interaction between biofilms and carbohydrates from the food on the tooth surface (host). The caused by the biofilm formation is the presence of colonies of Streptococcus mutans bacteria. To reduce the accumulation of biofilms, mechanical and chemical methods can be used, namely using a toothbrush and mouthwash. Probiotics are widely used in dentistry because they have anti-plaque activity and form H2O2 which can reduce biofilm formation. Purpose: The purpose of this study was to determine the effect of the probiotic Streptococcus sanguinis on the formation of Streptococcus mutans biofilm in artificial saliva. Method: This research method was experimental analytic with post test only group design. The samples were 9 and divided into 3 groups, namely the probiotic group, Streptococcus sanguinis, the positive control group chlorhexidine and the negative placebo control group. Each group incubated in adhesion phase for 4 hours at 37°C. The formation of biofilms is measured by calculating the average results of Optical Density using an ELISA-reader. Data analysis was performed using the test One-way Anova. Results: The average values of optical density in the S. sanguinis, chlorhexidine and placebo groups, in sequence, were 0.374, 0.414, and 0.420. One-way Anova test obtained a significant value (p) of 0.014 (p<0.05) which indicates that there are significant differences in the probiotic group Streptococcus sanguinis, the chlorhexidine group, and the placebo group. Conclusion: This study showed that the probiotic Streptococcus sanguinis had a significant impact on the formation of Streptococcus mutans biofilm in artificial saliva. Keywords : Biofilm, Optical Density, Probiotic, Streptococcus mutans, Streptococcus sanguinis

COMPARISON OF THE EFFECTIVENESS OF FERACRYLUM 1% AND FERACRYLUM 4% IN STOPING BLOODING AFTER GINGIVA INCISION Adilah Tsamarah; Erwid Fatchur Rahman; Rahmawati Sri Praptiningsih
Jurnal Medali Vol 4, No 2 (2022): Media Dental Intelektual September 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.2.163-169

Background: Bleeding is often found in dental practice. Excessive bleeding can hinder wound healing. For the surgeon to achieve rapid and effective hemostasis is important. Therefore, a topical hemocoagulation solution is given to help control surface bleeding by applying it locally. One such topical agent is Feracrylum. Feracrylum is a polymer of polyacrylic acid containing 0.05 to 0.5% iron. Feracrylum iron element will react with albumin and then convert water-soluble fibrinogen into water-insoluble fibrin which then forms a coagulum. This study aims to compare the effectiveness of Feracrylum 1% and Feracrylum 4% in stopping bleeding after gingival incision.Method: This study is a true experimental study that includes 3 treatment groups, namely the group without being given hemostatic agents, the group being given Feracrylum 1%, and the group being given Feracrylum 4%, each group consisting of 9 rats. Data were collected by calculating the bleeding time from the start of bleeding until the bleeding stopped after the gingival incision using a stopwatch and then recorded. Data analysis using Shapiro-Wilk, homogeneity test using Levene Test, nonparametric test using Kruskal-Wallis and Mann Whitney.Result: The results showed that the average bleeding time in the group without hemostatic agents, the group receiving 1% Feracrylum, and the group receiving Feracrylum 4% was 221 seconds, 103 seconds, and 50 seconds. The results of the Kruskal-Wallis and Mann Whitney showed that there was a significant difference in bleeding time between groups.Conclusion: The conclusion of this study is that the application of Feracrylum 4% was faster in stopping bleeding compared to the application of Feracrylum 1% after gingival incision in wistar rats.

COMPARISON OF THE NUMBER OF MACROPHAGES IN THE RAT REVERSIBLE PULPITIS AFTER APPLICATION OF BIODENTIN AND SIWAK EXTRACT Andina Rizkia Putri Kusuma; Rahmawati Sri Praptiningsih; Arlina Nurhapsari
Jurnal Medali Vol 4: Special Issue 1. Desember 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.3.6-11

Background: Oral disease still need special attention, especially in dentistry. Inflammation of the pulp is caused by the presence of irritants. One of the mechanical irritants is iatrogenic factors caused by operator error during preparation so that roof of pulp chamber is exposed. This condition is called reversible pulpitis/inflammation of the pulp. Cells that play a role during inflammation are macrophages which are the second defense cell after neutrophil apoptosis. A direct pulp capping procedure is indicated for this condition. The material commonly used is biodentin, but because the price tends to be expensive, alternative materials are needed. Siwak contains flavonoids which are known to play an important anti-inflammatory role in the healing of exposed pulp. The purpose of this study was to compare the number of macrophages in the dental pulp of rats with reversible pulpitis after administration of biodentin and siwak extract.Method: This research is a true experimental with a post test only group design, consisting of 2 treatment groups, namely the 75% siwak extract group and the biodentin group. The material is applied after the tooth has been prepared. The research sample used male wistar rats and sacrificed on the 3rd day. Samples were stained with Hematoxylin Eosin to see macrophage cells.Result: The data obtained were analyzed using the independent T-test. The test results showed a significant difference, with a value of p = 0.008 (p <0.05).Conclusion: The conclusion of this study that the siwak extract group showed a lower mean number of macrophage cells than the biodentin group.

CHEWING XYLITOL CANDY AND PROBIOTIC CANDY ON THE GROWTH OF DENTAL PLAQUE AND NUMBER OF COLONY OF STERPTOCOCCUS MUTANT BACTERIA Rahmawati Sri Praptiningsih; Rozaan Wakhid Amien; Rosa Pratiwi
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Caries or cavities is a disease in the hard tissue of the teeth characterized by damage to enamel and dentin caused by the metabolic activity of bacteria in plaque which can cause demineralization due to the products of microorganisms. Xylitol gum and probiotics can be used to reduce the plaque index and the number of Streptococcus mutans colonies. The purpose of this study was to compare the effect of chewing gum containing xylitol and probiotics on plaque growth and to determine the number of Streptococcus mutans colonies in students of the Faculty of Dentistry, Sultan Agung Islamic University.Method: This study used an experimental research method with a randomized posttest only control group design used 30 respondents divided into 3 groups, namely the xylitol, probiotics, and sugar groups. Each group had a good oral hygiene index (OHIS) and then checked the plaque index and the number of Streptococcus mutans colonies before and after chewing gum for 1 minute. Data were analyzed in the Laboratory of Microbiology, Faculty of Medicine, Sultan Agung Islamic University. Data were analyzed using paired t-test and Wilcoxon test. Result: The p value <0.05 or there was no significant decrease in each group. Conclusion: There is an effect of chewing gum containing xylitol and probiotics on the number of Streptococcus mutans and plaque index, but there is no significant difference between the plaque index and the number of Streptococcus mutans colonies.

COMBINATION OF DECIDUAL DENTAL STEM CELL AND PLATELET RICH PLASMA IN FIBROBLAST FORMATION IN THE JAWBONE REGENERATION OF WISTAR RATS Ade Ismail; Rahmawati Sri Praptiningsih; Muhammad naufal
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Orthodontic tooth movement is caused by mechanichal forces that cause pseudo-inflammation with the result that FGF (Fibroblast Growth Factors) was activated. The combination of SHED (Stem Cell From Human Exfoliated Deciduous Theeth) and PRP (Platelet Rich Plasma) hydrogels can affect the formation of fibroblast in the alveolar bone regeneration. The purpose of this study is the effect determination of SHED and PRP hydrogels for fibroblast formation in alveolar bone regeneration in wistar rats.Method: An experimental study was held using post-test only control group design. There were four groups, namely the PRP hydrogel group, the SHED hydrogel group, the SHED and PRP hydrogel group, and the povidone iodine group (n=8) treated with orthodontic tooth movement. Their mandibular tissue was made as histologycal slide, and the FGF expression were observed microscopically with 100x magnification. The data analyzed by ANNOVA and LSD test.Result: There was a significant effect of the combination of SHED and PRP hydrogel in ordering fibroblast in alveolar bone regeneration by ANNOVA test 0.001 (<0.05). This is because SHED and PRP are able to stimulate FGF expression through external stimulation.Conclusion: It concluded that the combination of SHED and PRP hydrogel affected fibroblast formation on alveolar bone regeneration in wistar rats

KECEMASAN MAHASISWA MENGHADAPI OBJECTIVE STRUCTURAL CLINICAL EXAMINATION (OSCE) Rahmawati Sri Praptiningsih
Odonto : Dental Journal Vol 3, No 2 (2016): December 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: OSCE is a clinical examination to test students’ skill. Students have to demonstrate skill possessed by the different conditions and examined by a particular examiners. The condition potentially the students suffering of anxiety in which symptoniced by phsichologies as students’ scared, felt nervous insecure. Impacts of anxiety caused reduction of students’ skill in pursuing their tasks thereby get the bad result .Purpose: To find out the correlation between anxiety and OSCE gradeMethod: This research is a quantitative descriptive research with cross sectional method,. Samples were taken from all second and third year students as many of 135 students who participated in OSCE. To investigate, the researcher used two kinds of research instruments, OSCE checklist and anxiety rating scale which were filled by students, then it was conducted statistics test using Pearson Product Moment correlation test.Results: Anxiety score in this research was about 73-141, with an average 100,66 and deviation standard 12,55, whereas OSCE grade showed that the lowest score was 40 and the highest was 92 with the average 71,5; deviation standard 10,52 and the probability > 0,001 (0,396>0,01). Conclusion: Most of students suffered nervous when facing OSCE. After analyzing through Pearson correlation test was obtained that there was not any positive correlation between students’ anxiety and OSCE grade. And there was significancy different between the first year and the third year

PENGARUH MENDENGARKAN AYAT SUCI AL QURAN TERHADAP TINGKAT KECEMASAN PASIEN SEBELUM TINDAKAN EKSTRAKSI GIGI Syafrudin Aulia Azhar; Rahmawati Sri Praptiningsih; Erna Dwi Agustin
Odonto : Dental Journal Vol 3, No 1 (2016): July 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Anxiety is a normal reaction is for the individual against a very pressing situation. Individuals who experience anxiety when visiting the dentist called Dental Anxiety. Dental anxiety due to bad experiences in previous treatments. Physiological signs of anxiety is increased pulse rate. Listening to the Quran can provide a relaxing effect so it can reduce anxiety. The purpose of this study to determine the effect of listening to the Koran on the anxiety level of the patient before tooth extraction.Method: Quasi experiment study with pre-posttest with control group design was conducted on 38 extraction patient and divided into two groups: a control group that did not listen to the Quran and the treatment group who listened to the Quran before extraction. The pulse rate of control groups were measured at the time when patients came in and 5 minutes later. The pulse ate of treatment group were measured at the time when patients came in and after the patient were listened to Quran. Data were analyzed by Independent T-Test .Result: The result showed the average pulse rate in the treatment group before and after were 67.26 and 62.68, while the average pulse rate in the control group before and after were 65.89 and 65.84. Independent T-test results showed p=0.000, it's mean that there are significant differences of the pulse rate between the treatment group and the control group on the anxiety level of the patient before tooth extraction.Conclusion: It can be concluded that listening to the Quran could reduce patient anxiety before dental extraction.

UJI EFEKTIFITAS EKSTRAK SIWAK (Salvadora Persica) BERBAGAI KONSENTRASI TERHADAP PEMBENTUKAN PLAK GIGI - Studi terhadap Murid MTsN Sale Ervina Diah Ruslinawati; Rahmawati Sri Praptiningsih; Siti Chumaeroh
Odonto : Dental Journal Vol 1, No 1 (2014): July 2014
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Nowdays many people who care about the health switching from chemical drugs to herbal medicine. There are many herbs that used for health. One is miswak (Salvadora persica) are many benefits to general health and dental health. Siwak can help inhibit dental plaque. Research purposes to determine effectiveness miswak extract against plaque formation. Method: The research was conducted 35 students of Madrasah Tsanawiyah Sale. The samples were divided into five groups, each group of 7 people to extract Siwak 25%, 50%, 75%, 100% and chlorheksidin group. Measurement of plaque index measurement using PHP Plaque Index (Patient Hygiene Performance Index) according Podshadley and Haley. Result: The criteria of dental plaque before rinsing dominant medium, with an average index plaque before rinsing 1,833 chlorheksidin group; 2.119 miswak extract 25%, 2,833 miswak extract 50%; 1,595 miswak extract 75%, and 2,905 miswak extract 100%. The criteria dominant plaque index after rinsing well, with an average index plaque after rinsing 1,476 chlorheksidin group; 1,286 miswak extract 25%; 0,929 miswak extract 50%; 0.690 miswak extract 75%, and 1,310 miswak extract 100%. P value of the one way ANOVA test after rinsing plaque index = 0.018 (p <0.05), means that the average index of dental plaque after rinsing significantly different. Post hoc test results showed 50% miswak extract group and 100% higher than chlorheksidin group, and 25% miswak extract and 75%. Conclusion: Concluded that miswak extract effective against dental plaque formation inhibition. Because the content of trimethylamine (TMA) has miswak which prevents sediment (deposit) particles on the surface of teeth.

PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS Laila Fitrotuz Zahroh; Rahmawati Sri Praptiningsih; Moh. Baehaqi
Odonto : Dental Journal Vol 1, No 1 (2014): July 2014
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

Co-Authors Ade Ismail Adilah Tsamarah Alma Linggar Jonarta Andina Rizkia Putri Kusuma Anggun Feranisa Arlina Nurhapsari Ayatusyifa' Maghfyrany Sumarno Aziz, Brahmadhia Satrio Elhakamy, Nurul Azkia Endah Aryati Eko Ningtyas Erna Dwi Agustin Ervina Diah Ruslinawati Erwid Fatchur Rahman Feranisa, Anggun Feranisa Grahita Aditya Hanifah Hasna Huda Heribertus Dedy Kusuma Yulianto Ichwan, Solachuddin Jauhari Arief Ikhsandarujati, Renanda Rifki Laila Fitrotuz Zahroh Liftia Layyinatus Syifa Moh. Baehaqi Muchaeroni, Isa Anshori Muhammad naufal Noviyanti, Shella Indri Nur Istighfah, Jafifah Mega Raden Mohamad Herdian Bhakti Rama Putranto Rifqi Wahyu Febrian Rina Kartika Sari Rizki Amalina Rosa Pratiwi, Rosa Rozaan Wakhid Amien S. Abdurrohman, Muhamat Muhtar Sari, Rina Kartika Sheilia Siwi Pranantri Siti Chumaeroh Syafrudin Aulia Azhar Taufiah Resa Ariana tazkia Yustisiani Nurafifah Widowati Siswomihardjo Yayun Siti Rochmah Yulistinawati, Yulistinawati

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