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REGENERASI SEL LIGAMEN PERIODONTAL DENGAN KOLAGEN SISIK IKAN NILA (Oreochromis niloticus) Adhalin Fuku Handini; Rosa Pratiwi; Tahta Danifatis Sunnah
Jurnal Medali Vol 3, No 1 (2021): Media Dental Intelektual September 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.v3i1.16767

Background : Regeneration of periodontal ligament cells with tilapia scales collagen (Oreochromis niloticus) is one of the therapies that can be used in the field of periodontics. This review aims to explain the mechanism of periodontal ligament cell regeneration using tilapia scales (Oreochromis niloticus) collagen.Methods : Literature collection is done by selecting journals or articles through PubMed, NCBI, and Google Scholar based on keywords that match the author's criteria.Results : Regeneration of cells using collagen from tilapia scales can be used by using a bone graft technique because fish collagen contains calcium and phosphate that resembles human, namely hydroxyapatite (HAP). Fish scales are an alternative source of type I collagen and have the potential to be an alternative material for bone graft.Conclusions : Regeneration of periodontal ligament cells using tilapia scale collagen can be done using bone graft techniques. Fish scales have many benefits apart from having almost the same content as humans as well as materials that are still easy to obtain and a more economical price to use. The authors suggest that the researchers further conduct further research on the preparation of tilapia scales so that they can be applied in tissue engineering actions in the field of periodonsia.

CHEWING XYLITOL CANDY AND PROBIOTIC CANDY ON THE GROWTH OF DENTAL PLAQUE AND NUMBER OF COLONY OF STERPTOCOCCUS MUTANT BACTERIA Rahmawati Sri Praptiningsih; Rozaan Wakhid Amien; Rosa Pratiwi
ODONTO : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.0.147-154

Background: Caries or cavities is a disease in the hard tissue of the teeth characterized by damage to enamel and dentin caused by the metabolic activity of bacteria in plaque which can cause demineralization due to the products of microorganisms. Xylitol gum and probiotics can be used to reduce the plaque index and the number of Streptococcus mutans colonies. The purpose of this study was to compare the effect of chewing gum containing xylitol and probiotics on plaque growth and to determine the number of Streptococcus mutans colonies in students of the Faculty of Dentistry, Sultan Agung Islamic University.Method: This study used an experimental research method with a randomized posttest only control group design used 30 respondents divided into 3 groups, namely the xylitol, probiotics, and sugar groups. Each group had a good oral hygiene index (OHIS) and then checked the plaque index and the number of Streptococcus mutans colonies before and after chewing gum for 1 minute. Data were analyzed in the Laboratory of Microbiology, Faculty of Medicine, Sultan Agung Islamic University. Data were analyzed using paired t-test and Wilcoxon test. Result: The p value <0.05 or there was no significant decrease in each group. Conclusion: There is an effect of chewing gum containing xylitol and probiotics on the number of Streptococcus mutans and plaque index, but there is no significant difference between the plaque index and the number of Streptococcus mutans colonies.

THE EFFECTIVENESS OF PHALERIA MACROCARPA’S LEAF NANOEMULSION GEL ON STAPHYLOCOCCUS AUREUS BIOFILM THICKNESS (IN VITRO) Rosa Pratiwi; Feny Nursyaputri; Recita Indraswary; Irma Dewi Ratnawati
ODONTO : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.0.69-79

Background: Periodontal disease often occurs in the oral cavity with 75.6-78.3% at the age of 35-44 years. One of the causes of periodontal disease is the accumulation of Staphylococcus aureus biofilm in the early colonization of the formation of the dental pellicle. Phaleria macrocarpa, also known as Gods Crown, is a traditional plant with antibacterial properties that can be used in the health sector. Nanoemulsion gel technology has the advantage of increasing the stability of the material. Aim the study to determine the ratio of the effectiveness of Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30% to decrease Staphylococcus aureus biofilm thickness.Method: This research method was in vitro experimental laboratory research using a post-test control design. Thirty samples were divided into five groups: Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30%, positive control using chlorhexidine gluconate 0.2% and negative control using aqua dest. Samples were incubated for 4 and 8 hours. Optical density readings were carried out to see the biofilm thickness after being given a Gods Crown leaves nanoemulsion gel Results: The mean of Phaleria macrocarpa’s leaf nanoemulsion gel, 30%, produced the lowest average optical density value. The incubation time of 4 hours resulted in a lower optical density value than 8 hours. The Kruskal-Wallis was p>0.05 showed there was no difference in biofilm thickness in each group.Conclusion: the effectiveness of the nanoemulsion group of Dewa crown leaf gel with a concentration of 30% was better than the 0.2% chlorhexidine gluconate gel and sterile distilled water group.

MULTIPLE GINGIVAL RECESSION COVERAGE TREATED WITH VISTA TECHNIQUE USING ACELLULAR DERMAL MATRIX (ADM) COMBINED WITH PLATELET RICH FIBRIN (PRF): A CASE REPORT Rosa Pratiwi; Ernie Maduratna Setiawatie
ODONTO : Dental Journal Vol 6, No 1 (2019): July 2019
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.6.0.56-61

Background: Gingival recession is an exposure of the root surface due to migration of the marginal periodontal tissues apical to the cemento enamel junction. There are so many root coverage procedures, among these techniques, VISTA technique combined with ADM and PRF offers minimally invasive approach.Case Management: A-40-year-old male visited Periodontics Clinic of DentalHospital Airlangga University with the chief complaint of poor aesthetic resulting from exposed root surfaces in upper right front region of the jaw. Root coverage by VISTA technique using ADM combined with PRF was planned to this case.Discussion: The minimal invasive VISTA technique approach, combined with ADMand PRF has a number of advantages for successful treatment of multiple recession defects. VISTA technique was used because it can maintain better blood supply and maintain critical papillary integrity. ADM works like an autogenous graft by providing a bioactive matrix consisting of collagen, elastin, blood vessel channels, and bioactive protein that support natural revascularization, cell repopulation, and tissue remodeling. Growth factors present in PRF plays a crucial role in hard and soft tissue repair.Conclusion: VISTA technique together with ADM and PRF membrane can besuccessfully used as a treatment method for multiple gingival recessions of Miller’s class I defects

PENGARUH APLIKASI GEL EKSTRAK MEMBRAN KULIT TELUR BEBEK 10% TERHADAP KEPADATAN SERABUT KOLAGEN PADA PROSES PENYEMBUHAN LUKA GINGIVA Agung Ikaputri Mulatpeni Novitasari; Recita Indraswary; Rosa Pratiwi
ODONTO : Dental Journal Vol 4, No 1 (2017): July 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.4.1.13-20

Background: Several processes in a teeth treatment often inflict wound ingingiva. Collagen is the key component of the wound healing. The collagen roles in advancing wound healing particularly, triggering the synthesis to induces collagen and cellular migration, and also inducing clotting cascades. Wound treatment using natural product is potential in accelerating wound healing, in particular duck egg membrane which contains collagen, glucosamine, and hyaluronic acid. This study aims to find out the influence of gel extract application of duck egg shell membrane 10 % towards collagen solidity on gingiva wound recovery process (study of Rattus norvegicus).Method: The subjects of the study consist of 20 male Rattus norvegicus aged 2-3 months which were given wound on labial gingiva of the maxillary central incisors with punch biopsy. The samples were divided into 2 groups, they were positive control group (Aloclair Gel®) and experimental group (gel extract of duck egg shell membrane 10%), each of them was treated topically twice a day for one minute.The assessment of collagen fiber was done by giving score over the collagen fiber solidity of the histological tissue through 6 points of view. The observer done by two person (blind interpretation) score of collagen fiber solidity.Result: The analysis result showed that there was a significant difference on collagen fiber solidity (p>0,05) between positive control groups and experimental group on the 3rd, 7th, and 14th day.Conclusion: This study concludes that the application of topical gel extract of duck egg shell membrane 10% affects the improvement of collagen fiber solidity on the process of healing gingiva wound on Rattus norvegicus

THE DIFFERENCE IN COLOR CHANGE OF PREHEATED AND NON-PREHEATED NANOFILLER COMPOSITE RESIN AFTER IMMERSION IN NON-INSTANT AND INSTANT COFFEE Sri Ainur Astutik; Benni Benyamin; Rosa Pratiwi
Jurnal Medali Vol 4, No 2 (2022): Media Dental Intelektual September 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/medali.4.2.123-129

Background: One of the aesthetic issues in using composite resin is the color change. This study aimed to compare the color changes of preheated and non-preheated nanofiller composite resin soaked in non-instant coffee and instant coffee.Method: This study involved 24 round composite resins with a diameter of 12 mm and a thickness of 2 mm. The samples were divided into two groups (n=12); group I was prepared at 68°C and group II was prepared at room temperature. After curing, the samples were immersed in distilled water at 37°C for 24 hours before being measured with a UV-vis spectrophotometer. Each group was divided into two sub-groups (n=6), one soaked in non-instant coffee and the other in instant coffee for three days, and the final color was measured. The data was analyzed using two independent t-tests with a significance level of α =0.05.Result: Two independent t-tests revealed a significant difference between preheated and non-preheated nanofiller composite resins soaked in non-instant coffee (p<0.05), as well as a significant difference between preheated and non-preheated nanofiller composite resins soaked in instant coffee (p<0.05).Conclusion: The preheating was effective to improve the color stability of composite resin after immersion in non-instant coffee and instant coffee.

EFFECT OF MANGOSTEEN PEEL EXTRACT (Garcinia mangosteen) TO FIBROBLAST NUMBER AS PERIODONTAL DISEASE THERAPY Adisty Restu Poetri; Rosa Pratiwi; Adhiatarika Rismadanti
Denta Journal Kedokteran Gigi Vol 17 No 1 (2023): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v17i1.1

Background: In Indonesia, periodontitis is among the most prevalent periodontal disease. The dentist may administer extra adjunctive therapy as a gel to treat inflammation and the bacterium that causes periodontitis. The antibacterial and anti-inflammatory xanthones included in mangosteen peel extract gel speed up the inflammatory phase. Objective: This study aimed to determine the effectiveness of mangosteen peel extract gel in increasing the number of fibroblast cells in the healing process of periodontitis in Wistar rats (Rattus norvegicus). Material and Method: This research was an experimental laboratory design with a post-test-only design divided into four groups, 50% mangosteen peel extract gel group, 75% mangosteen peel extract gel group, metronidazole gel group, and metronidazole plus gel group. Result: The research data were analyzed using the One Way ANOVA test and showed a significant value difference with a p=0.000 (p<0.05). However, in the inter-group test with Bonferroni post hoc, it was found that there was no significant difference between the 75% mangosteen peel extract gel group and the metronidazole gel group with a value of p = 0.709 (p>0.05). Conclusion: This study concludes that mangosteen peel extract gel effectively increases fibroblast cells' number in the periodontitis healing process.

Evaluation of Physical Stability of Phaleria Macrocarpa’s Leaf Extract Formulated in Nanoemulgel Rosa Pratiwi; Anggun Feranisa; Elsa Yuliana Sukmawati
Denta Journal Kedokteran Gigi Vol 17 No 1 (2023): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v17i1.2

Background: Phaleria macrocarpa’s leaf contains alkaloids, saponins, phenols, tannins, and flavonoids which have antioxidant, anti-inflammatory, and antibacterial activities. The nanoemulgel preparation was chosen because it can penetrate well and quickly into the mucosa thereby increasing absorption. Physical stability tests need to be carried out to ensure the quality, safety and benefits of the gel meet the expected specifications and stable during storage. Objective: This study aims to determine which concentration has the most stable physical stability of pH and viscosity which were observed for 28 days. Materials and Methods: This was an experimental laboratory design, with 30 preparation divided into 3 groups, group of 30%, 40%, and 50% concentration preparation of nanoemulgel leaves of Phaleria macrocarpa. which were tested for pH tests and viscosity tests for 28 days which was observed every 7 days in climatic chamber. The pH and viscosity tests among groups were compared and analyzed by the Repeated Anova and Pos Hoc test. Results: Average pH test and viscosity test 30% concentration with the average baseline and h+28 pH values were 5,02 and 4,97 and the baseline and h+28 viscosity values were 1610 cPas and 1520 cPas. The results of the average concentration of 50% pH value at baseline = 4,65 and h+28 = 4,60. The mean baseline viscosity value=1290 cPas and h+28=1170 cPas. The results of the post-Wilcoxon test showed p>0.05 in the 30% concentration group for pH and viscosity tests showed that there was no significant difference in pH and viscosity values at a concentration of 30%, It means the pH value and viscosity of the 30% concentration preparation of nanoemulgel were relatively constant from baseline to h+28. Conclusion: It can be concluded that the pH and viscosity test of the preparation of nanoemulgel leaves of Phaleria macrocarpa at a concentration of 30% was the most stable pH and viscosity compared to concentrations of 40% and 50%.

CHEWING XYLITOL CANDY AND PROBIOTIC CANDY ON THE GROWTH OF DENTAL PLAQUE AND NUMBER OF COLONY OF STERPTOCOCCUS MUTANT BACTERIA Rahmawati Sri Praptiningsih; Rozaan Wakhid Amien; Rosa Pratiwi
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Caries or cavities is a disease in the hard tissue of the teeth characterized by damage to enamel and dentin caused by the metabolic activity of bacteria in plaque which can cause demineralization due to the products of microorganisms. Xylitol gum and probiotics can be used to reduce the plaque index and the number of Streptococcus mutans colonies. The purpose of this study was to compare the effect of chewing gum containing xylitol and probiotics on plaque growth and to determine the number of Streptococcus mutans colonies in students of the Faculty of Dentistry, Sultan Agung Islamic University.Method: This study used an experimental research method with a randomized posttest only control group design used 30 respondents divided into 3 groups, namely the xylitol, probiotics, and sugar groups. Each group had a good oral hygiene index (OHIS) and then checked the plaque index and the number of Streptococcus mutans colonies before and after chewing gum for 1 minute. Data were analyzed in the Laboratory of Microbiology, Faculty of Medicine, Sultan Agung Islamic University. Data were analyzed using paired t-test and Wilcoxon test. Result: The p value <0.05 or there was no significant decrease in each group. Conclusion: There is an effect of chewing gum containing xylitol and probiotics on the number of Streptococcus mutans and plaque index, but there is no significant difference between the plaque index and the number of Streptococcus mutans colonies.

THE EFFECTIVENESS OF PHALERIA MACROCARPA’S LEAF NANOEMULSION GEL ON STAPHYLOCOCCUS AUREUS BIOFILM THICKNESS (IN VITRO) Rosa Pratiwi; Feny Nursyaputri; Recita Indraswary; Irma Dewi Ratnawati
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Periodontal disease often occurs in the oral cavity with 75.6-78.3% at the age of 35-44 years. One of the causes of periodontal disease is the accumulation of Staphylococcus aureus biofilm in the early colonization of the formation of the dental pellicle. Phaleria macrocarpa, also known as Gods Crown, is a traditional plant with antibacterial properties that can be used in the health sector. Nanoemulsion gel technology has the advantage of increasing the stability of the material. Aim the study to determine the ratio of the effectiveness of Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30% to decrease Staphylococcus aureus biofilm thickness.Method: This research method was in vitro experimental laboratory research using a post-test control design. Thirty samples were divided into five groups: Phaleria macrocarpa’s leaf nanoemulsion gel 10%, 20%, 30%, positive control using chlorhexidine gluconate 0.2% and negative control using aqua dest. Samples were incubated for 4 and 8 hours. Optical density readings were carried out to see the biofilm thickness after being given a Gods Crown leaves nanoemulsion gel Results: The mean of Phaleria macrocarpa’s leaf nanoemulsion gel, 30%, produced the lowest average optical density value. The incubation time of 4 hours resulted in a lower optical density value than 8 hours. The Kruskal-Wallis was p>0.05 showed there was no difference in biofilm thickness in each group.Conclusion: the effectiveness of the nanoemulsion group of Dewa crown leaf gel with a concentration of 30% was better than the 0.2% chlorhexidine gluconate gel and sterile distilled water group.

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