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All Journal International Journal of Renewable Energy Development BIOTROPIA - The Southeast Asian Journal of Tropical Biology ANNALES BOGORIENSES Jurnal Penelitian Kelapa Sawit Teknologi Indonesia Makara Journal of Technology Annales Bogorienses
Bambang Prasetya
Research Center for Biotechnology, LIPI, Cibinong, Bogor 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Veterinary

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Journal : Makara Journal of Technology

 1 
Utilization of Bagasse Cellulose for Ethanol Production through Simultaneous Saccharification and Fermentation by Xylanase Samsuri, M.; Gozan, Misri; Mardias, R.; Baiquni, M.; Hermansyah, Heri; Wijanarko, Anondho; Prasetya, Bambang; Nasikin, M.
Makara Journal of Technology Vol. 11, No. 1
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Utilization of Bagasse Cellulose for Ethanol Production through Simultaneous Saccharification and Fermentation by Xylanase. Bagasse is a solid residue from sugar cane process, which is not many use it for some product which have more added value. Bagasse, which is a lignosellulosic material, be able to be use for alternative energy resources like bioethanol or biogas. With renewable energy resources a crisis of energy in Republic of Indonesia could be solved, especially in oil and gas. This research has done the conversion of bagasse to bioethanol with xylanase enzyme. The result show that bagasse contains of 52,7% cellulose, 20% hemicelluloses, and 24,2% lignin. Xylanase enzyme and Saccharomyces cerevisiae was used to hydrolyse and fermentation in SSF process. Variation in this research use pH (4, 4,5, and 5), for increasing ethanol quantity, SSF process was done by added chloride acid (HCl) with concentration 0.5% and 1% (v/v) and also pre-treatment with white rot fungi such as Lentinus edodes (L.edodes) as long 4 weeks. The SSF process was done with 24, 48, 72, and 96 hour’s incubation time for fermentation. Variation of pH 4, 4,5, and 5 can produce ethanol with concentrations 2,357 g/L, 2,451 g/L, 2,709 g/L. The added chloride acid (HCl) with concentration 0.5% and 1% (v/v) and L. edodes can increase ethanol yield, The highest ethanol concentration with added chloride acid (HCl) concentration 0.5% and 1% consecutively is 2,967 g/L, 3,249 g/L. The highest ethanol concentration with pre-treatment by L. edodes is 3,202 g/L.
Immobilization of Saccharomyces Cerevisiae in Rice Hulls for Ethanol Production Martini, Edita; Andriani, Dian; GobiKrishnan, Sriramulu; Kang, Kyeong Eop; Bark, Surn-Teh; Sunwoo, Changshin; Prasetya, Bambang; Park, Don-Hee
Makara Journal of Technology Vol. 14, No. 2
Publisher : UI Scholars Hub

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The whole cell immobilization in ethanol fermentation can be done by using natural carriers or through synthetic carriers. All of these methods have the same purpose of retaining high cell concentrations within a certain defined region of space which leads to higher ethanol productivity. Lignocellulosic plant substance represents one of highly potential sources in ethanol production. Some studies have found that cellulosic substances substances can also be used as a natural carrier in cell immobilization by re-circulating pre-culture medium into a reactor. In this experiment, rice hulls without any treatment were used to immobilize Saccharomyces cerevisiae through semi solid state incubation combined with re-circulating pre-culture medium. The scanning electron microscopy (SEM) pictures of the carrier show that the yeast cells are absorbed and embedded to the rice hull pore. In liquid batch fermentation system with an initial sugar concentration of 50 g/L, nearly 100% total sugar was consumed after 48 hours. This resulted in an ethanol yield of 0.32 g ethanol/g glucose, which is 62.7% of the theoretical value. Ethanol productivity of 0.59 g/(L.h) is 2.3 fold higher than that of free cells which is 0.26 g/(L.h). An effort to reuse the immobilized cells in liquid fermentation system showed poor results due to cell desorption in the first batch which led to high sugar concentration inhibitory effect in the second batch fermentation. This might be solved by using semi solid fermentation process in the future work.
Digestibility of Betung Bamboo Fiber Following Fungal Pretreatment Fatriasari, Widya; Syafii, Wasrin; Wistara, Nyoman J.; Syamsu, Khaswar; Prasetya, Bambang
Makara Journal of Technology Vol. 18, No. 2
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This research evaluated the effect of fungal pretreatment of betung bamboo fibers and enzymatic- and microwaveassisted hydrolysis on the reducing sugar yield. The enzymatic hydrolysis of the pretreated biomass was carried out with cellulase and 10 and 20 FPU/g of substrate in a shaking incubator at 50 °C and 150 rpm for 48 h. The sulfuric acid concentration used in the microwave-assisted acid hydrolysis was 1.0, 2.5, and 5%, either with or without the addition of activated carbon. Microwave irradiation (330 Watt) was applied for 5–12.5 min. The yield of reducing sugar was better with the microwave-assisted acid hydrolysis, and the yield tended to increase with an increase in the irradiation time. Based on the dry weight of the initial biomass (bamboo), pretreatment with 5% inoculum loading resulted in a higher reducing sugar yield (17.06%) than with 10% inoculum loading (14.54%). At a 1% acid concentration, the formation of brown compounds decreased, followed by a reduction in the reducing sugar yield. The addition of activated carbon at a 1% acid concentration seemed to be of no benefit with respect to the yield in the microwaveassisted acid hydrolysis. The pretreatment with the 5% inoculum loading for 12.5 min at 1% acid concentration resulted in the highest reducing sugar yield. Under these conditions, the yield was 6.3-fold that of the reducing sugar yield using 20 FPU/g of cellulase. The rate of bamboo hollocellulose hydrolysis reached 22.75% of the maximum theoretical reducing sugar reducing sugar of dry biomass.
Co-Authors Aliyah, Novin Anas Miftah Fauzi Anja Meryandini Anondho Wijanarko Budi Mulyono, Ary Changshin Sunwoo, Changshin Dian Andriani, Dian Djumali Mangunwidjaja Don-Hee Park, Don-Hee Dulbert Tampubolon, Biatna Dwi Susmiarni, Rika Edita Martini, Edita Ellia Kristiningrum Euis HERMIATI Febrian Isharyadi Heri Hermansyah I Nyoman Jaya Wistara Kang, Kyeong Eop Khaswar Syamsu Kusharyoto, Wien Kusharyoto, Wien Kyeong Kang, Kyeong Lanjar Sumarno Lucky Risanto M Baiquni M Samsuri, M M. Baiquni Maggy T Suhartono Maggy Thenawidjaja Suhartono Mardias, R. Misri Gozan Mohammad Nasikin Nasikin, M. Nastiti Siswi Indrasti Ono Suparno R Mardias, R Restu Wahono, Daryono S Heris Anita, S Heris Samsuri, M. SHANTI RATNAKOMALA Sriramulu GobiKrishnan, Sriramulu Sunarti, Ttiti C Surn-Teh Bark, Surn-Teh Titi C. Sunarti Tjahyo Eka Darmayanti, Nur Utari Ayuningtyas Vorlop, Klaus Dieter Vorlop, Klaus Dieter Wasrin Syafii Widya FATRIASARI Wilke, Thomas Willke, Thomas Wulansari, Nuri YOPI YOPI