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All Journal The Indonesian Biomedical Journal Indonesian Journal of Obstetrics & Gynecology Science Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia)
Damar Prasmusinto
Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Indonesia, Ciptomangunkusumo Hospital, Jakarta
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Higher Trace Elements and Lower Fatty Acids Levels in Erythrocytes as Predictors of Preeclampsia Putri, Ruth Widhiati Raharjo; Prasmusinto, Damar; Wibowo, Noroyono; Irwinda, Rima; Purwosunu, Yuditiya; Saroyo, Yudianto Budi
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3256

Abstract

BACKGROUND: Preeclampsia is one of the common causes of maternal death in Indonesia. Many studies only focus on the diagnosis and pharmacological treatment of preeclampsia. However, predictors of preeclampsia need to be observed to add more focus on the etiology and prevention of preeclampsia. The imbalances of trace elements and fatty acids play an important role in preeclampsia. Therefore, this study was conducted to evaluate the status of trace elements and fatty acids in preeclampsia patients as predictors of preeclampsia.METHODS: A cross-sectional study was conducted in 3 hospitals, and involving 40 pregnant women classified into severe preeclampsia and normotensive groups. Trace elements and fatty acids were measured in serum and erythrocytes using Inductively Coupled Plasma and Gas Chromatography-Mass Spectrometry. Serum and erythrocytes fatty acid levels' cut-off value, sensitivity, and specificity were analyzed using Receiver Operating Characteristic (ROC) curve and Area Under the Curve (AUC) value.RESULTS: Serum selenium, manganese, and iron levels were significantly different in the preeclampsia group than in the controls (p<0.05). Of all the heavy metals, higher concentrations of cadmium, arsenic, lead, and mercury were found in preeclampsia groups compared to control. Linoleic acid showed the highest predictive value to increase severe preeclampsia with AUC of 0.8. The ratio of high omega-6/omega-3 increases the risk of preeclampsia.CONCLUSION: Selenium, manganese, iron, cadmium, arsenic, lead, and mercury levels are higher in the serum of preeclampsia patients. Almost all erythrocyte fatty acids were significantly higher in the control group compared to preeclampsia. Measurement of trace elements and fatty acids is needed as a predictor of preeclampsia. Erythrocyte fatty acids measurement is considered better than serum.KEYWORDS: trace elements, fatty acids, preeclampsia
Non-Severe Preeclampsia and Subclinical Inflammation: A Study of Cyclophilin A, NF-κB, PARP- 1, and Apoptosis in Human Placentas Resistantie, Novi; Wibowo, Noroyono; Prasmusinto, Damar; Jusman, Sri Widia Azraki; Yamin, Muhammad; Siregar, Nurjati Chairani; Prihartono, Joedo; Mose, Johannes Cornelius; Suhendro, Suhendro; Yunita, Ferdiana; Rosmanah, Lis; Margyaningsih, Nur Ita; Qotrunnada, Labibah; Roviqoh, Cindy Fawwaz; Rauf, Saidah
Indonesian Journal of Obstetrics & Gynecology Science Volume 8 Nomor 3 November 2025
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia.v8i3.947

Abstract

Objective: To compare the expression of CyP-A, NF-κB, PARP-1, and apoptotic index in Non-Severe Preeclampsia (NS-PE) and Normal Pregnancy (NP) and explore their roles in inflammation during preeclampsia.Methods: Conducted in Depok, Indonesia, the cross-sectional study involved 28 participants divided into NS-PE and NP groups based on ISSHP criteria. NP was defined as uncomplicated pregnancies at 38–40 weeks gestation. Placental weight was measured, and ELISA was used to assess biomolecule levels. Data were analyzed using T-tests or Mann-Whitney tests.Result: Maternal gestational age, body mass index, and leukocyte levels were significantly higher in NS-PE. The apoptotic index, measured by TUNEL assay, was also significantly elevated in NS-PE (41.56 ±24.87) compared to NP (23.96 ±18.79; p = 0.044). While CyP-A, PARP-1, and NF-κB levels were higher in NS-PE eventhough they were not statistically significant. Immunohistochemistry confirmed an overall increase in these molecules, supporting their clinical relevance.Conclusion: Despite the lack of statistical significance, increased inflammation and apoptosis in NS-PE may contribute to placental dysfunction and adverse pregnancy outcomes.Non-Severe Preeclampsia dan Inflamasi Subklinis: Studi CyP-A, NF-κB, PARP-1, dan Apoptosis pada Plasenta ManusiaAbstrak Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan ekspresi CyP-A, NF-κB, PARP-1, dan indeks apoptosis antara preeklamsia non-severe (NS-PE) dan kehamilan normal (NP), serta perannya dalam proses inflamasi pada preeklamsia.Metode: Metode penelitian yang digunakan dalam penelitian ini adalah cross-sectional. Penelitian ini dilakukan di Depok, Indonesia, dengan 28 partisipan yang dikelompokkan menjadi NS-PE dan NP berdasarkan kriteria ISSHP. Berat plasenta diukur dan kadar biomolekul dianalisis menggunakan ELISA. Uji T dan alternatif Mann-Whitney digunakan untuk analisis statistik.Hasil: Hasil penelitian menunjukkan bahwa usia kehamilan, indeks massa tubuh (IMT), dan kadar leukosit secara signifikan lebih tinggi pada NS-PE. Indeks apoptosis (TUNEL) juga lebih tinggi secara signifikan pada NS-PE (41,56 ±24,87) dibandingkan NP (23,96 ±18,79; p = 0,044). Kadar CyP-A, PARP-1, dan NF-κB lebih tinggi pada NS-PE meskipun tidak signifikan secara statistik, pemeriksaan IHK mengonfirmasi relevansi klinis peningkatan pada keseluruhan biomolekul tersebut. Kesimpulan: Meskipun signifikansi statistik rendah, peningkatan peradangan dan apoptosis pada NS-PE dapat menyebabkan disfungsi plasenta dan dampak buruk pada kehamilan.Kata kunci: Apoptosis; inflamasi; preeklamsia.
Placental Vitamin D, Oxidative Stress, and Senescence Markers in Spontaneous Preterm Birth: A Comparative Cross-Sectional Study Saroyo, Yudianto Budi; Wibowo, Noroyono; Prasmusinto, Damar; Purwosunu, Yuditiya; Irwinda, Rima; Dilmy, Mohammad Adya Firmansha; Putri, Atikah Sayogo; Mahendra, Arya Ivan; Putri, Lysandra Olivia Prasanti
Indonesian Journal of Obstetrics and Gynecology Volume 14. No. 1 January 2026
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v14i1.2928

Abstract

AbstractObjective: To evaluate the associations between vitamin D metabolism markers and biomarkers of oxidative stress and placental senescence among women with spontaneous preterm labor compared with those with term labor. Methods: We conducted a comparative cross-sectional study between 2017 and 2019 in two hospitals in Jakarta, Indonesia. Maternal serum and placental samples were collected from women with term labor and spontaneous preterm labor. Markers of the vitamin D pathway 25-hydroxyvitamin D (25[OH]D), 1,25-dihydroxyvitamin D? (1,25[OH]?D?), vitamin D receptor (VDR), and CYP27B1 along with oxidative stress (8-hydroxy-2--deoxyguanosine [8-OHdG]) and placental senescence markers (GLB1 and HMGB), were measured using ELISA, LC–MS/MS, and ICP–MS. Between-group comparisons were performed using parametric or nonparametric tests, as appropriate, and correlations were assessed using Pearson’s or Spearman’s correlation coefficients. Results: A total of 67 women were included (term labor, n = 34; spontaneous preterm labor, n = 33), and both groups were vitamin D deficient. Placental 1,25(OH)?D? levels were significantly lower in the preterm group than in the term group (4.58 ± 2.90 vs 5.57 ± 3.50 pg/ng, p = 0.037). Placental VDR levels also differed significantly between groups {21.70 (6.06–73.40) vs 16.48 (1.87–74.67), p = 0.041}. Across all participants, 8-OHdG and placental senescence markers were negatively correlated with placental 25(OH)D and 1,25(OH)?D? levels and positively correlated with placental CYP27B1 and VDR expression. Conclusion: In this comparative cross-sectional cohort, placental vitamin D metabolites were associated with lower levels of oxidative stress and placental senescence biomarkers, whereas VDR and CYP27B1 showed positive associations with these biomarkers. These patterns may reflect compensatory regulatory mechanisms in the context of maternal vitamin D deficiency. These findings are hypothesis-generating and warrant confirmation in prospective studies and mechanistic investigations. Keywords: oxidative stress, placental senescence, spontaneous preterm labor, vitamin D.
Co-Authors Bangun T. Purwaka, Bangun T. Dilmy, Mohammad Adya Firmansha Herman Kristanto Joedo Prihartono Johanes C. Mose Juneke J. Kaeng Jusman, Sri Widia Azraki Made K. Karkata, Made K. Mahendra, Arya Ivan Maisuri T. Chalid, Maisuri T. Makmur Sitepu, Makmur Margyaningsih, Nur Ita Mose, Johannes Cornelius Muhammad Yamin Noroyono Wibowo Nurjati Chairani Siregar Nuswil Bernolian Purwosunu, Yuditiya Putri, Atikah Sayogo Putri, Lysandra Olivia Prasanti Putri, Ruth Widhiati Raharjo Qotrunnada, Labibah Rauf, Saidah Resistantie, Novi Rima Irwinda, Rima Rosmanah, Lis Roviqoh, Cindy Fawwaz Saroyo, Yudianto Budi Suhendro Suhendro Yunita, Ferdiana Yusrawati Yusrawati