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Journal : Odonto dental journal

NUMBER OF BLACK-PIGMENTED ANAEROBIC BACTERIA CONTAINED IN THE GINGIVAL CREVICULAR FLUID (GCF) OF PATIENTS SUFFERING FROM THE CHRONIC PERIODONTITIS AND AGGRESSIVE PERIODONTITIS Depi Praharani; Peni Pujiastuti; Melok Aris Wahyukundari; Yuliana Mahdiyah Daat Arina; Desi Sandra Sari
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (574.238 KB) | DOI: 10.30659/odj.8.1.101-107

Abstract

THE EFFECTIVENESS OF USING TOOTHPASTE CONTAINING ROBUSTA COFFEE BEAN EXTRACT IN INHIBITING THE FORMATION OF DENTAL PLAQUE Rina Nanda Prasasti; Desi Sandra Sari; Peni Pujiastuti
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (490.412 KB) | DOI: 10.30659/odj.9.1.12-20

Abstract

Background: Periodontal disease is caused by the interaction between pathogenic bacteria and plaque, without the accumulation of plaque, periodontal disease will not appear. Plaque will be visible one to two days if no oral cleaning steps are taken. The combination of antimicrobial therapy such as antibiotics and mouthwash as well as mechanical therapy such as scaling and root plain gives more effective results. However, if done in the long term it will cause resistance. To overcome this, alternative materials are needed that are used to inhibit bacterial growth, one of which is robusta coffee beans. The content of robusta coffee bean extract contains phenolic and flavonoid compounds that function as antibacterials that work by disrupting the function of the cytoplasmic and cytoplasmic membranes of bacteria.Method: In this study, 6 groups were used, namely KN, K-, K+, KP 25%, KP 50%, and KP 75%. The gingivitis model was made with wire using ligation technique for 3 days, then toothpaste was applied, brushing process, and disclosing agent was given, and the plaque index calculation was PHP (Personal Hygiene Performance). The data obtained were then analyzed using the Kruskall-Wallis test followed by the Mann-Whitney test.Result: The Robusta coffee bean extract toothpaste group at a concentration of 75% experienced a significant reduction in plaque compared to the negative control group and the 25% treatment group (p<0.05), but there were no significant differences or had the same ability when compared to the experimental group. 50% treatment group, normal control group and positive control group.Conclusion: The 75% concentration of Robusta coffee bean extract found in toothpaste is proven to be effective in inhibiting the formation of dental plaque.
KADAR KALSIUM (Ca) DALAM CAIRAN KREVIKULAR GINGIVA PADA PENDERITA PERIODONTITIS KRONIS Ita Kurniawati; Peni Pujiastuti; Agustin Wulan Suci Dharmayanti
Odonto : Dental Journal Vol 2, No 2 (2015): December 2015
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.087 KB) | DOI: 10.30659/odj.2.2.11-16

Abstract

Background: Chronic periodontitis occurred mostly in productive ages. Chronic periodontitis was started by adherence and accumulation of plaque bacteria that caused vascular enhancement, PMN infiltration, and periodontal tissue destruction. This process caused bone calcium solubitily which went trough blood stream and excreted to gungiva sulci. Calcium in periodontal tissue could be found both in extracellular and intracellular matrix.Purpose: Purpose of this study was to know calcium level in gingival crevicular fluid of chronic periodontitis and compare calcium level in chronic periodontitis and gingivitis.Method: This study was analytic observational with cross sectional approach and got admission from ethic comission of Dental Faculty, Gadjah Mada University. The subjects were patients who came to dental hospital of Jember University. The subjects had to full fill informed consent. The subjects were selected according inclusion and exclusion criteria. Subjects  complying inclusion criteria were divided into gingivitis group (PI score 0 - 0.7) and chronic periodontitis (PI score 1 - 8.0). Gingival crevicular fluid samples were collected from teeth which got gingivitis and chronic periodontitis, GCF was absorbed using paperpoint and stored inside eppendorf tube. Paperpoint was added with 50 µL 0.02 M PBS pH 7.4 and 100 µL distilled water. Calcium level was measured by Atomic Absorption Spectrophotometer (AAS). Result: The result showed there was calcium in gingival crevicular fluid of chronic periodontitis. Mean of calcium level of chronic periodontitis was higher than  gingivitis.Conclusion: This study concluded there was calcium in gingival crevicular fluid of chronic periodontitis and calcium level of chronic periodontitis was higher than gingivitis.