Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Majalah Kedokteran Bandung Acta Biochimica Indonesiana YARSI Medical Journal Cermin Dunia Kedokteran
Prihandjojo Andri Putranto
Biochemistry Laboratory, Faculty Of Medicine, Sebelas Maret University, Surakarta
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
ENGARUH EKSTRAK PATIKAN KEBO (EUPHORBIA HIRTA L.) TERHADAP HITUNG EOSINOFIL DARAH TEPI PADA MENCIT MODEL ASMA ALERGI Heri Prasetyo, Diding; Putranto, R.P. Andri; Martini, Martini; Sarsono, Sarsono
Jurnal Kedokteran YARSI Vol 16, No 3 (2008): SEPTEMBER - DESEMBER 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.327 KB) | DOI: 10.33476/jky.v16i3.246

Abstract

Asthma is characterized by both chronic inflammation and remodeling of the airways. Eosinophils may contribute to inflammation associated with bronchial asthma by causing tissue damage and subsequently organ dysfunction, but also by the generation of cytokines and lipid mediators. It was reported in previous studies that pharmacological activities were identified in various compounds extracted fromEuphorbia hirta L. such as antiinflammatory,histamine inhibitor, lipoxygenase inhibitor, anti-allergic and cyclooxygenase inhibitor. This study was aimed to determine the effect of E. hirtaextract onperipheral blood eosinophils count in mice model of allergic asthma. MaleBalb/C mice were sensitized and challenged intraperitoneally (i.p) with ovalbumin(OVA). Mice were immunized i.p. on days 1 and 14 with 2.5 mgof OVA adsorbed to 7.75ml of Aluminium hydroxide gel. OVA challenges (10 mgOVA in l ml of PBS) were administered aerosolly on days 21, 23, 25 and 27, andperipheral blood eosinophilscount was determinedon day 28.A one-way analysis of variance (ANOVA) with LSDpost hocanalysis was used to determine significant differences. Results were expressed as mean+SEM, and values of p 0.05wereconsidered statistically significant. The results showed thatperipheral blood eosinophilscountin the OVA group was51.0+10.4cells/mm E. hirtaextract dose 500 mg/BW/day was30.2+6.3cells/mm3,E. hirtaextract dose 1,000 mg/BW/day was 30.4+6.0cells/mm and antihistamine group was32.2+8.6cells/mm respectively, whilecontrol mice showed46.6-8.8 cells/mm3. It was concluded that E. hirta extract could minimizeperipheralblood eosinophilscount.
Hepatoprotective and Antifibrotic Effects of Indonesian Propolis Diding Heri Prasetyo; Sarsono Sarsono; Ida Nurwati; Prihandjojo Andri Putranto; Martini Martini; Nabila Aushaf Prasetyo
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (901.973 KB) | DOI: 10.15395/mkb.v51n3.1768

Abstract

Liver cirrhosis is the irreversible stage in liver damage process which occurs after liver fibrosis due to necro-inflammatory activities and liver fibrosis. Therefore, inhibition of liver inflammation and fibrosis is very important to prevent liver cirrhosis. This study aimed to analyze the effect of ethanol extract of propolis (EEP) from mount Lawu, Indonesia to prevent liver damage and fibrosis progression in mice with hepatic cirrhosis. This study was performed during the period of June 2018 to May 2019 on a sample of 32 male Balb/C mice divided into control group (P1), induction of carbon tetrachloride (CCl4 ) group (P2), induction of 50 mg/BW CCl4 + EEP group (P3), and (induction of 100 mg/KgBW CCl4 + EEP (P4) with each group consisted of eight mice. The CCl4 in olive oil was administered intraperitoneally three times a week for six weeks. Mean differences between group was determined using ANOVA test with a significance level of 0.05. The induction of CCl4 increased liver cell damage and serum alanin aminotransferase (ALT) level. However, the addition of EEP significantly (p<0.001) reduced liver cell damage as seen in P3 (54.38±4.17 per 100 liver cells) and P4 (37.13±4.36 per 100 liver cells) groups and serum alanin aminotransferase (ALT) as seen in P3 (291.19±113.92 U/L) and P4 (229.38±73.45 U/L) groups. The APRI scores were also reduced after EEP as seen in P3 (0.738±0.292) and P4 (0.513±0.253) groups. Thus, EEP isolates from Gunung Lawu can reduce liver cell damage and fibrosis in mice model of hepatic cirrhosis. Efek Hepatoprotektif dan Antifibrotik Propolis IndonesiaSirosis hati (SH) merupakan tahap ireversibel dalam proses kerusakan hati yang terjadi setelah fibrosis hati sebagai hasil aktivitas nekroinflamasi dan fibrosis hati. Oleh karena itu, penghambatan inflamasi hati dan fibrosis sangat penting untuk mencegah terjadi SH. Penelitian ini bertujuan menganalisis efek ekstrak etanol isolat propolis (EEP) gunung Lawu dalam mencegah progresifitas kerusakan dan fibrosis hati pada mencit model SH. Penelitian dilakukan di Laboratorium Histolgis Fakultas Kedokteran Universitas Sebelas Maret Surakarta dan Lembaga Penelitian dan Pengujian Terpadu (LPPT) Universitas Gajah Mada Yogyakarta . Penilitian ini dilakukan periode Juni 2018 sampai Mei 2019, dengan sampel 32 ekor mencit Balb/C jantan dibagi menjadi kelompok (P1) kontrol negatif, (P2) induksi karbon tetraklorida (CCl4 ), (P3) induksi CCl4 +EEP dosis 50 mg.kgBB-1, dan (P4) induksi CCl4 +EEP dosis 100 mg.kgBB-1, dengan tiap-tiap sampel terdiri dari delapan ekor mencit Balb/C jantan. CCl4 dalam minyak zaitun diberikan pada tikus Balb/C tiga kali seminggu selama enam minggu secara intraperitoneal. Uji ANOVA digunakan untuk menentukan perbedaan rata-rata antar kelompok dengan tingkat kemaknaan sebesar 0,05. Namun, penambahan EEP secara signifikan (p<0,001) menurunkan tingkat kerusakan sel hati seperti yang terlihat pada P3 (54,38±4,17 per 100 sel hati) dan kelompok P4 (37,13±4,36 per 100 sel hati) dan serum alanin aminotransferase (ALT) seperti yang terlihat dalam kelompok P3 (291,19±113,92 U / L) dan P4 (229,38±73,45 U / L). Hasil skor APRI menunjukkan kelompok P1(0,213±0,113) dan P2 (0,863±0,534), EEP dapat menurunkan skor APRI pada kelompok P3 (0,738±0,292) dan P4 (0,513±0,253). Simpulan, EEP isolat Gunung Lawu menurunkan tingkat kerusakan sel hati dan fibrosis pada mencit model SH.
Pengaruh Ekstrak Buah Naga Merah (Hylocereus polyrhizus) terhadap Kadar HDL Tikus Wistar Model Sindrom Metabolik: Hasil Penelitian Valensia Putri Melliana; Jarot Subandono; Danus Hermawan; R. Prihandjojo Andri Putranto; Sarsono
Cermin Dunia Kedokteran Vol 51 No 10 (2024): Infeksi
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v51i10.1145

Abstract

Introduction: Metabolic syndrome is characterized by hypertension, central obesity, insulin resistance, and dyslipidemia. Hylocereus polyrhizus is known for its potential in treating metabolic syndrome. Method: Laboratory experimental study with pretest-posttest control group design. Thirty rats were divided into 5 groups: K1 and K2 as negative and positive control, K3, K4, and K5 as groups receiving red dragon fruit extract at doses of 60, 120, and 180 mg/200 gBW. Data were analyzed using one-way ANOVA, Tukey HSD test, paired T-test, and Pearson correlation. Results: The highest HDL levels were observed in K5, while the lowest was in K1. Pearson correlation revealed a strong relationship between the dose of Hylocereus polyrhizus and HDL levels. Conclusion: Hylocereus polyrhizus extract could significantly increase HDL level. In this study, the optimal dose was 180 mg/200 gBW.
Dose-dependent anticholesterol effects of Fuji apple simplicia in a rat model of metabolic syndrome Himawan Pramudana Amiruddin; R. Prihandjojo Andri Putranto; Amalina Shabrina; Veronika Ika Budiastuti
Acta Biochimica Indonesiana Vol. 9 No. 1 (2026): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.233

Abstract

Background: Metabolic syndrome-associated dyslipidemia increases cardiovascular disease risk. Fuji apples (Malus domestica) contain bioactive compounds that may modulate cholesterol metabolism. Objective: To evaluate dose-dependent anticholesterol effects of Fuji apple simplicia in metabolic syndrome rats. Methods: Thirty male Wistar rats were allocated into five groups (n=6): negative control, positive control (metabolic syndrome without treatment), and three treatment groups receiving simplicia at 150, 300, or 450 mg/200 g body weight/day for 28 days. Metabolic syndrome was induced using high-fat diet and streptozotocin-nicotinamide injection. Total cholesterol was measured using enzymatic colorimetric assay and analyzed using mixed ANOVA. Results: Simplicia produced significant dose-dependent cholesterol reductions compared to positive control (p<0.001). Reductions were 71.77 mg/dL (34.09%), 100.53 mg/dL (47.78%), and 112.02 mg/dL (52.75%) at doses of 150, 300, and 450 mg/200 g body weight/day, respectively. Conclusion: Fuji apple simplicia exhibits significant anticholesterol activity in metabolic syndrome rats through a dose-dependent mechanism, supporting its potential as a complementary intervention for hypercholesterolemia management in metabolic syndrome.
Co-Authors . Martini . Sarsono ., Muthmainah Aflifia Birruni Sabila Amalina Shabrina Bela Dirk Danus Hermawan Diding H P Diding Heri Prasetyo Dwiyono, Rizqi Ahmad Nur FK UNS, Murkati FK UNS, Murkati Hari Purnomo S Heri Prasetyo, Diding Herlina Kusuma Dewi Himawan Pramudana Amiruddin Huda, Khairunnisa Nurul Ida Nurwati Jarot Subandono Kharisman, Ibnu Kusumawati, Ratna Martini Martini Martini Martini Mulyono, Rosi Dwi Nabila Aushaf Prasetyo Putra, Michael Sophian Rohmaningtyas Hidayah Setyaningrum Rustam Sunaryo Sarsono Sarsono Sarsono Sarsono Sarsono Sri Hartati H Valensia Putri Melliana Veronika Ika Budiastuti