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Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.

PEMBERDAYAAN IBU-IBU PKK DAN ANGGOTA JEMAAT ADVENT WAMENA MELALUI PEMBUATAN TELA-TELA SINGKONG Tulak, Alber; Paling, Sepling; Inrianti; Meiky Tuwo
Jurnal Abadimas Adi Buana Vol 7 No 02 (2024): Jurnal Abadimas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/abadimas.v7.i02.a8507

Pengabdian Masyarakat melalui Program Kuliah Kerja Nyata Sekolah Tinggi Ilmu Pertanian Petra Baliem Wamena, dapat direspon baik oleh Ibu-Ibu PKK dan Jemaat Gereja Advent Wamena Kota, serta mahasiswa yang terlibat dalam kegiatan ini. Tujuan dalam kegiatan ini adalah salah satu usaha diversifikasi pangan, artinya sebagai usaha untuk mengatasi masalah ketergantungan pada satu bahan pangan pokok saja, misalnya dengan mengolah umbi - umbian menjadi berbagai bentuk makanan yang mempunyai rasa khas dan tahan lama untuk disimpan. Metode pelaksanaan kegiatan ini dimulai dari melakukan persiapan rencana kegiatan, sosialisasi pelaksanaan kegiatan, pelatihan pembuatan tela-tela dan pengemasannya, melakukan monitoring dan evaluasi hasil kegiatan. Hasil kegiatan ini dapat memberikan pengetahuan baru terhadap masyarakat dalam mengolah hasil pertanian berupa umbi-umbian seperti singkong menjadi tela-tela yang memiliki nilai jual yang lebih tinggi. Melalui kegiatan ini masyarakat ibu-ibu PKK dan Jemaat Gereja Advent Wamena Kota memiliki pengetahuan untuk dapat memanfaatkan hasil pertanian berupa singkong yang ada di wilayah pengunungan tengah Papua yang memiliki banyak manfaat, diolah untuk menciptakan produk-produk turunannya seperti tela-tela dari singkong. Prospek usaha tela-tela dapat memberikan pendapatan keluarga yang lebih baik, karena memiliki sasaran penjualan seperti anak sekolah yang suka dengan cemilan dengan harga yang terjangkau

UTILIZATION OF YOUTUBE-BASED DIGITAL MEDIA IN SCIENCE LEARNING IN ELEMENTARY SCHOOLS Sepling Paling
International Journal of Teaching and Learning Vol. 2 No. 5 (2024): MAY
Publisher : Adisam Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar

Digital media is a means of delivering information from the sender to the recipient. YouTube videos as a learning medium are very suitable for the learning characteristics of today's generation of students, so students prefer to watch learning videos via YouTube. Using YouTube media for learning, especially science learning, can help students develop critical thinking skills, solve problems, make decisions, and use social media to collaborate. YouTube can provide students and teachers with valuable experiences for freedom of expression, educational collaboration, and skill development. Innovating YouTube videos to teach science, they must be systematic, concrete and complex. The content of YouTube content contains four main elements in the nature of IPA. YouTube video innovations that suit the nature of science learning are carried out in the form of five types of videos, namely (1) concept explanation videos, (2) scientific facts videos, (3) model simulation videos, (4) demonstration videos and (5) experimental videos. Learning videos on YouTube can be used for interactive learning in the classroom, both for students and teachers themselves through online and offline presentations.

EFEKTIVITAS EKSTRAK DAUN DIWOKA (Piper miniatum Bl.) SEBAGAI PESTISIDA NABATI DALAM MENGENDALIKAN HAMA ULAT TRITIP (Plutella xylostella) PADA TANAMAN KUBIS Inrianti, Inrianti; Paling, Sepling; Mosip, Erinus; Berliana, Meri; Pian, Hendrik Yulius; Walila, Maktalina
AGROTEKSOS, Jurnal Ilmiah Ilmu Pertanian Vol 34 No 3 (2024): Jurnal Agroteksos Desember 2024
Publisher : Fakultas Pertanian Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/agroteksos.v34i3.1294

This study aims to examine the effect of various concentrations of Diwoka leaf extract (Piper miniatum Bl.) on the mortality of diamondback moth (Plutella xylostella) on cabbage (Brassica oleracea var. capitata L.) in a laboratory scale. Diwoka leaf extract was applied at concentrations of 40%, 50%, 60%, 70%, 80%, and 90%, with the mortality of the diamondback moth observed after five hours. The results showed that the higher the concentration of Diwoka leaf extract, the higher the mortality rate of the diamondback moth. At a concentration of 90%, mortality reached 97.50%, while at 40%, mortality was only 32.50%. These findings indicate that Diwoka leaf extract can function as an effective botanical pesticide in controlling diamondback moth on cabbage plants. This study contributes significantly to the development of alternative natural pesticides that are environmentally friendly and have the potential to be applied in sustainable agriculture.

PENGENDALIAN ULAT GRAYAK (Spodoptera litura F.) PADA TANAMAN UBI JALAR (HIPERE) MENGGUNAKAN EKSTRAK DIWOKA (Piper miniatum Bl.) SEBAGAI SALAH SATU TANAMAN ENDEMIK PEGUNUNGAN TENGAH PAPUA, INDONESIA Inrianti, Inrianti; Paling, Sepling; Murib, Les
Jurnal Inovasi Pendidikan dan Sains Vol 3 No 3 (2022): Desember
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Nahdlatul Wathan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51673/jips.v3i3.1193

One type of pest that has begun to invade several types of plants in Jayawijaya Regency including hypere plants is the Fall Armyworm pest (Spodoptera litura F.). Armyworms are not the primary (main) pest on hypere plants. However, it is a secondary pest because it can attack several types of plants. However, the occurrence of a population explosion will cause higher damage to the attacked plants. The aim of this study was to determine the effect of various concentrations of diwoka leaf extract (Piper miniatum Bl.) on the mortality of Grayak caterpillar (Spodoptera litura F.) on sweet potato (Hipere) and the extract concentration which was more effective in controlling Grayak caterpillar pests (Spodoptera litura F.) in sweet potato plant (Hipere). The treatments tried were: 1. Control (sterile water application), 2. Diwoka leaf application 40%, 50%, 60%, 70%, 80%, 90% through food (stomach poison). The experiment was arranged in a completely randomized design with four replications each using 250 instar larvae of Spodoptera litura.F. Observations showed that there was an effect of each concentration tested on Spodoptera litura.F through food (stomach poison). Of the six treatments of diwoka leaf extract which was applied during the preliminary test on the fifth instar with a concentration of 70% showed a significant effect on the mortality rate of Spodoptera litura.F. After conducting a preliminary test and then carrying out further tests on the second instar larvae where in that instar the attack rate was very high, so the concentration used was 40%-90%. All concentrations showed a significant effect on second instar larvae, at a concentration of 40% the mortality rate was 12.50%, and the highest concentration was 90% with a mortality rate of 80%.

Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.

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