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Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.

Health Education For Early Awareness in Maintaining Oral Health For Students at PPTQ Hasan Munadi Islamic Boarding School, Ponorogo, East Java: Edukasi Peningkatan Kesadaran Sejak Dini Santri Santriwati dalam Menjaga Kesehatan Gigi dan Mulut, Pondok Pesantren PPTQ Hasan Munadi, Ponorogo, Jawa Timur Puspitasari, Yeni; Alam, Sabda; Desi, Febria; Artanty, Dian; Anindya, Cinitra; Cahyo, Adi; Zahratur, Anindita; Fitri, Bingah; Mustriana, Ratna; Kusuma, Febriyanti; Yulviyanti, Anita; Sutowijoyo, Aryo; Surya, Rangga; Rahmatari, Bandaru; Nerito, Prima; Dewita, Meirna; Gayatri, Khamila
Journal of Community Empowerment for Multidisciplinary (JCEMTY) Vol. 2 No. 2 (2024): November 2024
Publisher : KHD Production

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53713/jcemty.v2i2.269

PPTQ Hasan Munadi is an Islamic boarding school located in Badengan District, Ponorogo, East Java. The journey to the school requires a long travel distance, with winding roads and a considerable distance from the city. This situation leads to limited healthcare facilities, particularly dental clinics, and infrequent oral health education activities in the surrounding area. As a result, many students at the boarding school have a limited understanding of the importance of maintaining oral health, including the proper techniques for brushing teeth. The aim of the health education program at Pondok Pesantren PPTQ Hasan Munadi is to provide dental and oral health education and training to students from an early age (7-9 years old). This health education program used pre-post test to asses the program’s success. Before the health education intervention, students are given a pre-test. Following this, an educational session is conducted using a lecture method supported by media such as a phantom, video, and flipchart. Next session, a post-test is administered to assess any changes in the students' knowledge about maintaining oral health. Data analysis reveals that 49% of the students had a moderate level of knowledge regarding dental and oral health before the intervention. After the educational session, there was an increase of 7.3% in the number of students who achieved a good level of knowledge. This program indicates a significant improvement in the students' knowledge regarding oral health following the educational intervention.

Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.

Lactobacillus Sp. Di Rongga Mulut: Patogen Atau Probiotik? Rahmatari, Bandaru; Wahyuni, Ratna; Alfedo, James Kevin; Winarno, Indah Dwi; Sari, Meirna Dewita; Mustriana, Ratna
Jurnal Medika Malahayati Vol 9, No 3 (2025): Volume 9 Nomor 3
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jmm.v9i3.21440

Lactobacillus sp. menunjukkan peran ganda sebagai patogen dan probiotik dalam rongga mulut. Beberapa spesies tertentu, seperti L. casei, L. paracasei, dan L. rhamnosus, sering dikaitkan dengan perkembangan karies gigi karena kemampuannya sebagai asidogenik dan pembentuk biofilm, yang memungkinkan berkembang biak dengan baik di lingkungan dengan pH rendah dan berkontribusi pada demineralisasi gigi. Sebaliknya, spesies lain seperti L. crispatus, L. salivarius, dan L. gasseri menunjukkan sifat probiotik yang signifikan, termasuk penghambatan patogen oral utama (misalnya, Streptococcus mutans dan Porphyromonas gingivalis), pengurangan inflamasi, dan peningkatan imunitas mukosa mulut. Strain probiotik Lactobacillus dapat memulihkan keseimbangan mikroba, menghasilkan zat antimikroba seperti asam organik, hidrogen peroksida, dan bakteriosin, serta membentuk biofilm pelindung yang membatasi kolonisasi patogen. Dampak Lactobacillus pada rongga mulut sangat bergantung pada strain dan konteksnya, dengan beberapa strain meningkatkan kesehatan mulut sementara yang lain dapat memperburuk penyakit. Dengan demikian, aplikasi probiotik Lactobacillus yang ditargetkan memberikan harapan untuk mencegah dan mengelola penyakit mulut, meskipun penelitian lebih lanjut diperlukan untuk mengklarifikasi mekanisme dan memastikan keamanan secara klinis.

ANALISIS PENERAPAN PRINSIP HACCP DAN IMPLIKASINYA TERHADAP MUTU GIZI PRODUK OLAHAN LAUT UMKM DI KENJERAN SURABAYA Sari, Meirna Dewita; Saputro, Andre Ridho; Rahmatari, Bandaru
JURNAL SVASTA HARENA RAFFLESIA Vol 4 No 2 (2025): Volume 4 Nomor 2 Desember 2025
Publisher : Poltekkes Kemenkes Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33088/shr.v4i2.1145

Seafood-based micro, small, and medium enterprises (MSMEs) play a vital role in the local economy of Kenjeran, Surabaya, yet many still lack sufficient knowledge of food safety systems. This study aimed to assess the understanding of MSME actors regarding the seven principles of the Hazard Analysis and Critical Control Points (HACCP) system and its implications for the nutritional quality of processed seafood products. A quantitative descriptive method was used, involving 24 closed-ended questionnaire items distributed to seafood-processing MSMEs in Kenjeran through purposive sampling. The research was conducted from January to March 2025. The instrument was validated through expert review and tested using Pearson correlation and Cronbach’s Alpha for reliability (α = 0.93). Data analysis employed descriptive statistics to identify patterns of understanding across HACCP principles. Results showed that overall comprehension was moderate, with the highest mean score on Principle 6 (Verification: 3.36), followed by Principles 2, 3, 4, and 7. However, Principle 1 (Hazard Analysis: 2.97) and Principle 5 (Corrective Actions: 2.98) had the lowest average scores, indicating critical gaps in recognizing food hazards and responding to deviations. These weaknesses may negatively impact the nutritional quality of seafood products, including protein degradation and vitamin loss due to poor control measures. It is recommended that targeted technical training, expert mentoring, improved documentation systems, and government support be implemented to enhance HACCP adoption. Strengthening food safety practices in MSMEs not only ensures product quality but also increases competitiveness in both local and global markets.

Education on Improving Dental Health Maintenance for Students : Edukasi Peningkatan Pemeliharaan Kesehatan Gigi Santri Yulviyani, Anita; Sabda Alam; Febria Desi; Dian Artanty; Cinitra Anindya; Adi Cahyo; Anindita Zahratur; Bingah Fitri; Ratna Mustriana; Febriyanti Kusuma; Aryo Sutowijoyo; Rangga Surya; Bandaru Rahmatari; Prima Nerito; Meirna Dewita; Yeni Puspitasari; Khamila Gayatri; Angeline Musmarihan
Journal of Community Empowerment for Multidisciplinary (JCEMTY) Vol. 3 No. 1 (2025): May 2025
Publisher : KHD Production

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53713/jcemty.v3i1.299

Dental and oral health problems, especially tooth decay, are diseases experienced by almost half of the world's population. Indonesia's most significant dental problems are damaged/cavities/painful teeth. One of the promotive efforts to maintain and improve the degree of dental and oral health in the community is through counseling. International Islamic Boarding School (IIBS) Baitul Manshurin Malang which does not yet have health service facilities. Health education programs are rarely conducted by healthcare workers or the local health department. Therefore, a community service program was realized at IIBS Baitul Manshurin Malang in the form of counseling aimed at increasing knowledge about dental and oral health maintenance. 350 The male and female students took a pre-test first to assess their knowledge, attitudes, and practices regarding dental health maintenance. Conducted counseling and concluded with a post-test to determine whether there was an improvement in the knowledge, attitudes, and actions of the male and female students. The pre-test data shows that 45.1% of the students at IIBS Baitul Manshurin fall into the moderate category. The post-test results show an increase in the percentage of the good category, from 13.1% to 20.6%, and a decrease in the percentage of the bad category, from 41.7% to 26%. The outreach activities have a positive impact on the knowledge, attitudes, and actions of the students at IIBS Baitul Manshuri.

Health Education For Early Awareness in Maintaining Oral Health For Students at PPTQ Hasan Munadi Islamic Boarding School, Ponorogo, East Java: Edukasi Peningkatan Kesadaran Sejak Dini Santri Santriwati dalam Menjaga Kesehatan Gigi dan Mulut, Pondok Pesantren PPTQ Hasan Munadi, Ponorogo, Jawa Timur Yeni Puspitasari; Sabda Alam; Febria Desi; Dian Artanty; Cinitra Anindya; Adi Cahyo; Anindita Zahratur; Bingah Fitri; Ratna Mustriana; Febriyanti Kusuma; Anita Yulviyanti; Aryo Sutowijoyo; Rangga Surya; Bandaru Rahmatari; Prima Nerito; Meirna Dewita; Khamila Gayatri
Journal of Community Empowerment for Multidisciplinary (JCEMTY) Vol. 2 No. 2 (2024): November 2024
Publisher : KHD Production

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53713/jcemty.v2i2.269

PPTQ Hasan Munadi is an Islamic boarding school located in Badengan District, Ponorogo, East Java. The journey to the school requires a long travel distance, with winding roads and a considerable distance from the city. This situation leads to limited healthcare facilities, particularly dental clinics, and infrequent oral health education activities in the surrounding area. As a result, many students at the boarding school have a limited understanding of the importance of maintaining oral health, including the proper techniques for brushing teeth. The aim of the health education program at Pondok Pesantren PPTQ Hasan Munadi is to provide dental and oral health education and training to students from an early age (7-9 years old). This health education program used pre-post test to asses the program’s success. Before the health education intervention, students are given a pre-test. Following this, an educational session is conducted using a lecture method supported by media such as a phantom, video, and flipchart. Next session, a post-test is administered to assess any changes in the students' knowledge about maintaining oral health. Data analysis reveals that 49% of the students had a moderate level of knowledge regarding dental and oral health before the intervention. After the educational session, there was an increase of 7.3% in the number of students who achieved a good level of knowledge. This program indicates a significant improvement in the students' knowledge regarding oral health following the educational intervention.

Education on Improving Dental Health Maintenance for Students : Edukasi Peningkatan Pemeliharaan Kesehatan Gigi Santri Anita Yulviyani; Sabda Alam; Febria Desi; Dian Artanty; Cinitra Anindya; Adi Cahyo; Anindita Zahratur; Bingah Fitri; Ratna Mustriana; Febriyanti Kusuma; Aryo Sutowijoyo; Rangga Surya; Bandaru Rahmatari; Prima Nerito; Meirna Dewita; Yeni Puspitasari; Khamila Gayatri; Angeline Musmarihan
Journal of Community Empowerment for Multidisciplinary (JCEMTY) Vol. 3 No. 1 (2025): May 2025
Publisher : KHD Production

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53713/jcemty.v3i1.299

Dental and oral health problems, especially tooth decay, are diseases experienced by almost half of the world's population. Indonesia's most significant dental problems are damaged/cavities/painful teeth. One of the promotive efforts to maintain and improve the degree of dental and oral health in the community is through counseling. International Islamic Boarding School (IIBS) Baitul Manshurin Malang which does not yet have health service facilities. Health education programs are rarely conducted by healthcare workers or the local health department. Therefore, a community service program was realized at IIBS Baitul Manshurin Malang in the form of counseling aimed at increasing knowledge about dental and oral health maintenance. 350 The male and female students took a pre-test first to assess their knowledge, attitudes, and practices regarding dental health maintenance. Conducted counseling and concluded with a post-test to determine whether there was an improvement in the knowledge, attitudes, and actions of the male and female students. The pre-test data shows that 45.1% of the students at IIBS Baitul Manshurin fall into the moderate category. The post-test results show an increase in the percentage of the good category, from 13.1% to 20.6%, and a decrease in the percentage of the bad category, from 41.7% to 26%. The outreach activities have a positive impact on the knowledge, attitudes, and actions of the students at IIBS Baitul Manshuri.

Integrated Non-Communicable Disease Prevention Program at Wreda Mandiri Posyandu for Older Adults: Pencegahan Penyakit Tidak Menular Terintegrasi dengan Posyandu Lansia Wreda Mandiri Sartika Puspita; Dwi Aji Nugroho; Edwyn Saleh; Talitha Khairunnisa; Bandaru Rahmatari; Sinta Noer Hudawati
ABDIGI : JURNAL PENGABDIAN MASYARAKAT KESEHATAN GIGI Vol. 4 No. 1 (2026): April 2026
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Non-Communicable Diseases (NCDs) are chronic diseases that cannot be transmitted from one person to another. The aging population is at greater risk of developing NCDs, such as hypertension, diabetes mellitus, and cardiovascular disease. Several factors, including genetics, physiology, environment, and lifestyle, can cause these diseases. Poor lifestyle habits, not balanced with physical activity and a healthy diet, significantly increase the prevalence of NCDs in a region. In Indonesia, 73% of deaths are caused by NCDs, and the Special Region of Yogyakarta and Jakarta provinces have the highest prevalence of NCDs. Therefore, a community service program was implemented to educate the older adults and increase their knowledge about NCDs. This program was integrated with the Wreda Mandiri Posyandu for Older Adults, an Integrated Health Service Post in Yogyakarta using interactive educational methods. The pre-test and post-test results given to 12 participants showed a significant increase in knowledge scores. This outreach and mentoring program can be continued regularly to improve the quality of life for older adults. Keyword : Non-communicable diseases; Older Adults; Counseling.

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