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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Menara Perkebunan BIOVALENTIA: Biological Research Journal Jurnal Pusat Inovasi Masyarakat
Megayani Sri Rahayu
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences

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Identification of metabolites for biomarker of nitrogen and potassium use efficiency in oil palm Setiowati, Retno Diah; Sri Wening; Nuringtyas, Tri Rini; Rahayu, Megayani Sri; Sudarsono, Sudarsono
Menara Perkebunan Vol. 93 No. 1 (2025): 93(1), 2025
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i1.631

Abstract

Nutrient-use efficiency in oil palm is important for economic and environmental reasons. This research aimed to identify biomarkers to discriminate between tolerant and susceptible oil palms to potassium (K) and nitrogen (N) deficiency. A screening of oil palm materials for N or K use efficiency was conducted using an omission trial experiment, where only targeted nutrient was applied as treatment, while all other nutrients were applied as recommended. The treatment was performed in the main nursery for ten months to identify progenies with contrasting traits. Metabolite analysis was performed to identify specific metabolites as biomarkers for N-efficient and K-efficient palms. Samples taken from the roots of the contrasting progenies were treated with liquid nitrogen prior to grinding into a powder for liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis. The LC-HRMS analysis showed 277 metabolites from K and N treatments after data trimming, which were then analysed in MetaboAnalyst 6.0 for biomarker identification. The results showed that some metabolites were statistically significant. Metabolites identified in more than one analysis have a higher likelihood of being considered as biomarkers. In this experiment, we compared PLS-DA, sPLS-DA, and Random Forest. However, some identified metabolites were not to occur naturally in the treatment palms. Some amino acids and antioxidants were promising biomarkers to differentiate the N-deficiency-tolerant and K-deficiency-tolerant palms. Thus, the biomarkers facilitate the breeding scheme to create a nutrient-efficient palm planting material.
Studi Tahap Perkembangan Mikrospora dan Respon Pepaya Hermaprodit Kultivar Callina melalui Kultur Antera Halimah Widyaningrum; Darda Efendi; Diny Dinarti; Megayani Sri Rahayu
Jurnal Hortikultura Indonesia (JHI) Vol. 16 No. 2 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.2.77-83

Abstract

Kultur antera merupakan teknik kultur in vitro yang menjanjikan dalam mendapatkan tanaman haploid dalam satu generasi. Penelitian ini bertujuan mendapatkan informasi mengenai tahap perkembangan mikrospora dan respon antera pada tiga ukuran bunga hermaprodit pepaya Callina pada medium induksi kalus. Penelitian dilakukan di Laboratorium Mikroteknik dan Kultur Jaringan 3, IPB- Bogor, dari Juli hingga November 2022. Bahan tanam yang digunakan adalah antera dari tiga ukuran bunga hermaprodit pepaya kultivar Callina meliputi kecil (10-15 mm), sedang (16-20 mm) dan besar (21-25 mm). Medium kultur yang digunakan adalah medium MS dengan enam perlakuan ZPT yaitu empat perlakuan kombinasi (0.005, 0.01, 0,1 dan 0,5 mg L-1) CPPU dan (0.1 mg L-1) NAA; 0.1 mg L-1 BAP dan 0.1 mg L-1 NAA; serta 1.0 mg L-1 BAP dan 2.0 mg L-1 NAA. Rancangan percobaan menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) satu faktor (stadia perkembangan mikrospora) dan 2 faktor (induksi kalus). Hasil penelitian menunjukkan bahwa bunga berukuran 10-15 mm memiliki persentase mikrospora uninucleate tertinggi (77.95%), merupakan ukuran yang responsif untuk menginduksi terbentuknya kalus dari antera. Perlakuan 0.5 mg L-1 CPPU dan 0.1 mg L-1 NAA menunjukkan persentase antera membentuk kalus tertinggi (40.4%). Sementara itu, ukuran bunga tidak berpengaruh nyata terhadap parameter yang diamati, tetapi pada ukuran bunga 10-15 mm menunjukkan persentase antera membentuk kalus paling banyak (13%).
Co-Authors , Krisantini Adrian Ramdani Afifah Farida Jufri Agus Purwito Ananda Diah Oktaviani Asep Setiawan Asih, Ni Putu Sri Awang Maharijaya Bambang Suhartawan Daawia, Daawia dan Sudarsono Dede Kismantoro Dhiya Nabilla Ardhani Diny Dinarti Efendi, Darda Erna Siaga, Erna Erna Sinaga Fitriyah Nurul Flowrentyka Ferona Haloho Frederico Tunggal Gina Rahma Utami Halimah Widyaningrum Haris Aprianto Setiawan Hasrat Enggal Prayogi Iskandar Lubis Ismail Maskromo Juang Gema Kartika Jufri, Afifah Farida Matra, Deden D. Maudy Susanti Ni Made Armini Wiendi Novita Niken Putri Ulayyah Nurhalim Nyssa Azaria Dewani Ratna Andriani Setia Kurniawan Setiawan, Asep S Setiowati, Retno Diah Shafira Puti Nazihah Sri Wening Sudarsono Titiek S. Yuliani Tri Rini Nuringtyas Utami, Gina Rahma Visasti , Khurul