, Krisantini
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor

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Evaluation of Commercial Sunflower (Helianthus annuus L.) Cultivars in Bogor, Indonesia for Ornamental and Nursery Production Aisyah, Syarifah Iis; Khotimah, Khotimah; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 1 No 1 (2014): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.436 KB) | DOI: 10.29244/jtcs.1.1.13-16

Abstract

A wide range of imported ornamental sunflower seeds have been marketed in Indonesia. A field evaluation was conducted on seven sunflower cultivars to investigate their germination, growth and development in humid tropical environment in Bogor, West Java, Indonesia and to determine their potential uses. Most of the tested cultivars, except for ‘Sungold Double’ and ‘Velvet Queen’, demonstrated good germination and growth. ‘Eclipse’ was highly susceptible to stem rot, which resulted in only 30% of the plants survived. ‘Little Leo’, ‘Teddy Bear’ and ‘Sungold Double’ were naturally compact, dwarf, and produced attractive blooms so they might be suitable as pot plants. ‘Hallo’, ‘Velvet Queen’, ‘Sunburst’, and ‘Eclipse’s plant height were more than 100 cm, had long stem and large blooms. These cultivars might be suitable as landscape plants.Keywords: morphological characteristics, West Java, pot plants
Proliferation of Protocorm-Like Bodies of Dendrobium mannii and Dendrobium mirbelianum in Chitosan-containing Media In Vitro Rahmah, Syifaur; Wiendi, Ni Made Armini; Suwarno, Willy B.; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 2 No 3 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.289 KB) | DOI: 10.29244/jtcs.2.3.22-28

Abstract

Dendrobium mannii and Dendrobium mirbelianum were rare and endangered orchid species. In vitro micro propagation could be used to conserve and proliferate these species. The aims of this study were to determine the effects of chitosan on proliferation of the protocorm-like-bodies (PLBs) of in vitro grown Dendrobium mannii and D. mirbelianum. Chitosan has been reported as an important component to promote orchid growth in vitro. In this study chitosan was included in the in-vitro media at the concentrations of 0, 1, 2, 3, and 4 mg.L-1. The highest PLBs formation, clump diameter, and fresh weight on D. mannii were obtained from explants cultured on medium supplemented with 1 mg.L-1 chitosan. After 14 weeks of culture, fresh weight, clump diameter and average number of PLBs of D.mannii were 29.52 %, 33.00 % and 35.88% greater at chitosan (1 mg.L-1) compared to control media.  D. mirbelianum treated with low chitosan concentrations (1 and 2 mg.L-1) had 65.71% more leaves than those without chitosan treatment. Chitosan, however, had an adverse effects on growth of both species at  4 mg.L-1. Medium contain 4 mg.L-1 chitosan induced PLBs proliferation of D.mannii, but inhibited PLBs proliferation of D.mirbelianum.  These results suggested that chitosan had important roles in PLB proliferation and could be used for the propagation of D. mannii PLBs in vitro. Keywords: rare orchid species, in-vitro
Characterization of 13 Accessions of Purslane (Portulaca sp.) from Bogor, West Java, Indonesia Setiawan, Farensa Ikman Dedi; Aisyah, Syarifah Iis; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 3 No 3 (2016): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (750.326 KB) | DOI: 10.29244/jtcs.3.3.67-74

Abstract

Characterization is part of crop the breeding process aims to protect biodiversity and preserve local germplasm. Indonesia has two known species of Portulaca, Portulaca oleracea and P. grandiflora. Thirteen Portulaca accessions have been collected from Bogor area, comprising five accessions of Portulaca oleracea and eight accessions of Portulaca grandiflora. This study was aimed at the characterization of the 13 accessions and was undertaken at Indoflower BLST, Bogor, between October 2015 to February 2016. The results showed a significant difference in the color of flowers between the two species of Portulaca. The most significant difference is the overall appearance of P. oleracea species. Character color and shape of the flowers between the P. grandiflora white (GW) and P. oleracea are similar.  Significant differences between the species exist in relation to the root systems, but the root systems of accessions within each species are generally similar.  The branching characteristics between the two species and among accessions are generally similar, with branches emerging through the base of leaves or nodes.   However, there are differences in the leaf characteristics between the two species. This study has provided information on flower characteristics of P. grandiflora and P. oleraceae which will be valuable for future breeding efforts in Portulaca. 
Radiosensitivity Levels of In Vitro Cultured Celosia cristata Planlets by γ - Ray Irradiation Hayati, Dhieni; Aisyah, Syarifah Iis; -, Krisantini
Journal of Tropical Crop Science Vol 3 No 2 (2016): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.893 KB) | DOI: 10.29244/jtcs.3.2.61-65

Abstract

Plantlets of the ornamental plant Celosia cristata were irradiated with gamma rays to increase their genetic diversity. This study was aimed to establish  the lethal levels of gamma radiation  (LD20, LD30 and LD50) for  C. cristata plantlets.   The irradiation doses used were 0, 25, 50 and 75 Gy. The growth of irradiated plantlets was evaluated to the third generation. Irradiated C. cristata MV1 plantlets showed a decrease in growth, with plantlets irradiated at 75 Gy showing only 30% survival.   Abnormal growth characteristics observed  in the  third generation plantlets included  the shortening of internodes, and curling of leaves.  LD50, LD30 and LD20 of C. cristata were 68.73 Gy, 46.68 Gy and 35.65 Gy, respectively.
Evaluation of Growth, Flowering and Seed Morphology of Batflower, Tacca chantrieri Andre Ajisyahputra, Nikko Rizky; Palupi, Endah Retno; Krisantini, Krisantini; Wiendi, Ni Made Armini; Mulyono, Joko
Journal of Tropical Crop Science Vol 4 No 2 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.221 KB) | DOI: 10.29244/jtcs.4.2.64-69

Abstract

Tacca chantrieri is a perennial plant belongs to Taccaceae family that has the potential to be marketed as an ornamental plant due to its unique shape and dark color inflorescence. To date, very limited publication on T. chantrieri are available. The aim of this research was to examine plant growth pattern and flower phenology of T. chantrieri from West Borneo, Indonesia, and from Queensland, Australia in relation to their potential development as ornamental pot plants. The study was extended to examine T. chantrieri seed morphology with an inclusion of an accession from Thailand.  The field experiment was conducted at the Leuwikopo experimental station in Darmaga, Bogor, Indonesia. Seed morphology examination was conducted at the Histology Laboratory of Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. T. chantrieri from West Borneo blooms six times a year whereas the Queensland accession twice a year. West Borneo accession had smaller bractea, fewer numbers of flowers per inflorescence and shorter flower stalk than Queensland accession. In addition, T. chantrieri Queensland grew faster than Borneo with the one leaf formed every two to three weeks, whereas it was at least four weeks in the Borneo accession. Both accessions were susceptible to leaf brown spot disease caused by Cercospora taccae. T. chantrieri Queensland seeds are oval dark brown, whereas West Borneo and Thailand accessions were shaped like kidneys (reniform) with a lighter brown color. This study demonstrated that there is great diversity in T. chantrieri including plant morphology, seed shape and color, which are important for species identification and for development of T. chantrieri as ornamental pot plant.
In Vitro Mutagenesis of Etlingera elatior by Gamma Ray Intermittent Irradiation Azzahra, Elmi Irmayanti; Aisyah, Syarifah Iis; Dinarti, Diny; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.45 KB) | DOI: 10.29244/jtcs.5.3.111-118

Abstract

Etlingera elatior is tropical ornamental plant commonly called “torch ginger” from Zingiberaceae family. Conventional breeding of E. elatior is limited by cross incompatibility, poor fruit set and low seed production.  In this study irradiation of E. elatior with Gamma ray performed to induce mutation. This study was aimed to increase morphological diversity and to obtain unique morphological characters to increase the aesthetic value of E. elatior as ornamental plants and cut flower. Two genotypes of E. elatior, red and white flowers, were tested. The LD20, LD35, and LD50 were determined following intermittent (split dose) Gamma irradiation with a two-hour gap between each gamma ray shot. Red genotype E. elatior explants were irradiated with dose of 3 + 3 Gy (LD20); 4 + 4 Gy (LD35) and 5 + 5 Gy (LD50); white genotype were irradiated with a dose of 2 + 2 Gy (LD20); 2.8 + 2.8 Gy (LD35) and 3.7 + 3.7 Gy (LD50). Non-irradiated explants were set as control.  The results of this study indicated that the increase in dose of gamma ray irradiation changed shoot length and number of leaves in the red genotype per explant as compared to control. Morphological changes occurred in leaf shape at 5 + 5 Gy and 3.7 + 3.7 Gy and formation of variegated leaves at 2.8 + 2.8 Gy and 5 + 5 Gy. Keywords: mutation breeding, ornamental plant, split irradiation dose, torch ginger, Zingiberaceae
Aplikasi Berbagai Komposisi dan Konsentrasi Pupuk Majemuk untuk Pembentukan Kantong pada Nepenthesx ventrata Romy Agus Saputro; Sintho Wahyuning Ardie; . Krisantini
Buletin Agrohorti Vol. 1 No. 1 (2013): Januari 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.923 KB) | DOI: 10.29244/agrob.1.1.113-118

Abstract

Nepenthes is a genus of carnivorous plants in the monotypic family Nepenthaceae. The genus comprises around 130 species, numerous natural and many cultivated hybrids. Plants generally need fertilizer to promote their growth and development. However, in their original habitat Nepenthes forms pitchers under nutrition starvation condition. This study aimed at determining the effect of fertilization on the growth and pitcher formation of Nepenthesx ventrata. Experiment was conducted at Suska Nursery, Caringin, West Java, Indonesia from February until June 2011. Plants were treated with the N: P: K, 60-30-30, 32-10-10 and 10-55-10 and at different concentrations ie, 0.5, 1 and 2 g L-1. Fertilizer was applied weekly to the growing media. Weekly observations were conducted on number of leaves, plant height, time of pitchers initiation, the number of pitchers and pitcher diameter and length. Daily temperature, relative humidity and EC (electrical conductivity) of the growing media were also recorded during the experiment. The results showed that combination of fertilizer with different composition and concentration did not affect Nepenthes growth and development. Application of fertilizer resulted in smaller size pitchers compared to control plants. Future research on Nepenthes should be conducted in a longer period since Nepenthes is a slow growing plants.Keywords: Nepenthesx ventrata, fertilizer, pitcher plant
Proliferasi In Vitro Plb Anggrek Dendrobium lasianthera Hasil Induksi Mutasi Genetik dengan Kolkisin Melalui Penambahan Benzyl Adenine Gilar Bawonoadi; Ni Made Armini Wiendi; . Krisantini
Buletin Agrohorti Vol. 5 No. 2 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (705.327 KB) | DOI: 10.29244/agrob.v5i2.16791

Abstract

Dendrobium lasianthera merupakan spesies anggrek yang endemik di Papua. Proliferasi atau perbanyakan tanaman melalui kultur jaringan merupakan salah satu alternatif dalam memperbanyak spesies tanaman dengan jumlah individu yang terbatas. Keragaman karakteristik pada tanaman hias, baik yang bersifat alami atau hasil dari induksi mutasi, dianggap penting dalam menentukan nilai ekonomisnya. Pengembangan protokol produksi membutuhkan penelitian terkait media yang sesuai untuk memperbanyak tanaman. Penelitian dilakukan dengan mengkulturkan eksplan anggrek Dendrobium lasianthera berupa plb (protocorm-like body) dari anggrek D. lasianthera yang telah diinduksi mutasi melalui perendaman dalam larutan mutagen kolkisin pada penelitian sebelumnya. Pengamatan dilakukan terhadap kemampuan pertumbuhan 960 eksplan dari 16 kombinasi perlakuan (termasuk kontrol) perendaman kolkisin ditumbuhkan pada media MS0 yang ditambahkan sitokinin BA (6-Benzyladenine) sebanyak 1 mgL-1 dan 2 mgL-1. Hasil penelitian menunjukkan bahwa eksplan dengan kombinasi perlakuan perendaman yang berbeda memiliki kemampuan pertumbuhan yang berbeda secara nyata, dilihat dari jumlah daun, akar, plb dan tunas baru yang terbentuk selama periode pengamatan.Perbedaan dalam konsentrasi BA dalam media tidak menunjukkan respon yang berbeda nyata dalam jumlah daun dan akar baru yang terbentuk, namun nyata meningkatkan jumlah tunas baru yang terbentuk dan mempercepat pembentukan tunas. Eksplan yang ditumbuhkan pada media BA 1 mgL-1 memiliki rata-rata waktu awal pembentukan tunas yang lebih singkat dibandingkan dengan media BA 2 mgL-1. Beberapa planlet hasil induksi mutasi menunjukkan perbedaan fenotipe dari planlet kontrol berupa bentuk daun yang berbeda.
Budidaya, Panen, dan Pascapanen Philodendron giganteum, Philodendron selloum, dan Philodendron xanadu di Jawa Barat Assyifa Fauzia; Dr. Dewi Sukma; Krisantini
Buletin Agrohorti Vol. 10 No. 3 (2022): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v10i3.46455

Abstract

Kegiatan dilaksanakan dari 18 Januari hingga 17 Mei 2021. Penelitian bertujuan mengevaluasi teknik budidaya, panen, dan pascapanen daun potong Philodendron giganteum, Philodendron selloum, dan Philodendon xanadu. Metode yang digunakan adalah metode langsung dan tidak langsung. Pertumbuhan Philodendron mengalami kenaikan setiap minggunya dilihat dari kenaikan rata-rata tinggi tanaman tiap minggu sebesar sebesar 1.4 cm pada Philodendron selloum serta 1,8 cm pada Philodendron giganteum dan Philodendron xanadu. Jumlah daun pada ketiga jenis Philodendron yang diamati bertambah satu helai setiap minggunya. Pemanenan daun potong dilakukan menggunakan sistem manual dan penanganan pascapanen daun potong menggunakan metode penyimpanan basah. Kata kunci: Daun potong, Philodendron, produksi, tanaman hias
Photoautotrophic System: A Review and Potential Application for Plant Propagation In Vitro Krisantini Krisantini; Ni Made Armini Wiendi
Journal of Tropical Crop Science Vol. 5 No. 2 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.007 KB) | DOI: 10.29244/jtcs.5.2.73-78

Abstract

AbstractThe standard method of in vitro plant micro propagation uses of tightly closed culture bottles using agar media containing macro and micro nutrients and sucrose as a source of carbon for the explants. The closed bottle culture is usually kept in a temperature and light controlled environment which is lower and of different quality from the natural sunlight, resulting in high relative humidity and no air exchange inside the bottles.  Explants produced in vitro have malfunctioned stomata, undeveloped cuticles and lower leaf chlorophyll levels, and hyper hydration of the plantlets. Photoautotrophic tissue culture is micro propagation without or with a reduced sugar level in the culture media, so the growth or accumulation of carbohydrates of the explants is dependent fully upon photosynthesis and inorganic nutrient uptake. This method is usually combined with ventilation or CO2 enrichment, and recently, with incorporating porous materials such as vermiculite, gum or paper pulp to the agar media to promote better root system of the explants. This article discuss the advantages and disadvantages of the photoautotrophic micro propagation compared to the standard micro propagation methods, and provided the results of the photo autotrophic micro propagation studies conducted at Laboratory of Tissue Culture II of the Department of Agronomy and Horticulture, Bogor Agricultural University, Indonesia.