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All Journal Biospecies Jurnal Biologi Universitas Andalas Journal of Information Systems Engineering and Business Intelligence Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL AGRONIDA Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Pengabdian kepada Masyarakat Nusantara Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Bioscientist : Jurnal Ilmiah Biologi Agroradix : Jurnal Ilmu Pertanian Dharma: Jurnal Pengabdian Masyarakat Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat International Journal of Renewable Energy Development Bioeksperimen Biodiversitas Journal of Biological Diversity
Feskaharny Alamsjah
Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Social Sciences Other

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Isolation and Characterization of Protease-Producing Thermophilic Bacteria from Padang Dama Hot Spring, Solok, Indonesia Mellanie Alia Putri; Anthoni Agustien; Yetti Marlida; Feskaharny Alamsjah
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20118

Abstract

This study aimed to isolate, screen, and partially characterize protease-producing thermophilic bacteria from the Padang Dama hot spring, Solok, Indonesia. The main issue addressed in this study is the limited information regarding thermophilic bacteria capable of producing protease enzymes from this geothermal environment, despite their potential use in industrial processes requiring stable enzymes. Isolation was carried out using Nutrient Agar followed by incubation at 50 °C for 24 hours. Screening of protease activity was performed using Skim Milk Agar based on clear zone formation, and the proteolytic index (PI) was calculated to evaluate enzyme activity. Selected isolates were further characterized through macroscopic, microscopic, and biochemical analyses. The results showed that three isolates exhibited proteolytic activity, with isolate TUA-109 showing the highest proteolytic index, indicating superior enzymatic potential compared to other isolates. Partial characterization revealed that isolate TUA-109 is a Gram-negative, rod-shaped bacterium with positive catalase activity and motility, which are important traits supporting its adaptability and potential for enzyme production under extreme conditions. These findings highlight the potential of thermophilic bacteria from the Padang Dama hot spring as sources of protease enzymes, with isolate TUA-109 identified as a promising candidate for further investigation, particularly for industrial enzyme production. Future studies are recommended to optimize enzyme production and evaluate its application in industrial processes.
Smart System for Mushroom Identification and Classification: A Systematic Literature Review Indra Laksmana; Muhammad Ilhamdi Rusydi; Feskaharny Alamsjah
Journal of Information Systems Engineering and Business Intelligence Vol. 12 No. 2 (2026): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jisebi.12.2.489-506

Abstract

Background: Mushrooms play an essential role in ecological balance and significantly contribute to economic activities. However, the close visual resemblance between edible and toxic species continues to cause fatal poisoning incidents worldwide. Conventional identification approaches rely heavily on expert judgment, making them subjective, time-consuming, and unsuitable for large-scale or real-time use. Recent developments in artificial intelligence (AI), particularly deep learning techniques, have created new opportunities for developing automated and reliable identification systems. Objective: This study systematically reviews recent research on intelligent technologies for mushroom identification and classification. This study maps the main research objectives, data types, morphological features, technologies, algorithms, evaluation practices, and remaining challenges, with particular attention to toxicity classification and safety-critical decision support. Methods: A systematic literature review was conducted following the PRISMA 2020 protocol and the PICOC framework. We screened publications indexed in Scopus between 2021 and 2025. From 1,308 initial records, 90 high-quality studies were selected for detailed analysis. Results: The analysis of the selected studies reveals that the majority of research focuses on discriminating between edible and poisonous mushrooms (52 studies), predominantly using visual characteristics related to shape (64 studies) and color (59 studies). Deep learning techniques, especially convolutional neural networks (CNNs) (40 studies) and vision transformers (11 studies), dominate the field and frequently report classification accuracies exceeding 95%. Despite these achievements, several challenges persist, including the difficulty of fine-grained classification among visually similar species, limited dataset availability, and performance degradation in complex natural environments. Conclusion: Although AI-based approaches have considerable potential to support mushroom identification, their reliability is limited by reliance on visual data alone when species are morphologically similar. Future studies should place greater emphasis on lightweight models for field deployment, multimodal sensing, risk-aware evaluation, and Explainable AI (XAI) so that intelligent systems can be used more safely and transparently in real-world contexts.   Keywords: Mushroom Identification, Artificial Intelligence, Deep Learning, Food Safety, Systematic Literature Review
Short Communication: Evaluation of antibacterial activities and phytochemical composition of ethanolic extract of Diplazium esculentum FESKAHARNY ALAMSJAH; SASQIA FANDINI; MILDAWATI MILDAWATI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250304

Abstract

Abstract. Alamsjah F, Fandini S, Mildawati M. 2024. Short Communication: Evaluation of antibacterial activities and phytochemical composition of ethanolic extract of Diplazium esculentum. Biodiversitas 25: 937-941. Antibacterial agents are crucial in fighting infections by stopping or inhibiting pathogenic bacteria growth. Diplazium esculentum (Retz.) Sw., is a natural resource with great potential in traditional medicine. This study aimed to investigate the antibacterial effectiveness of ethanol extract of D. esculentum against two common bacteria, Pseudomonas aeruginosa and Staphylococcus epidermidis. This research was done using disc diffusion technique with comprehensive phytochemical screening. The results revealed antibacterial properties of the ethanolic extract of D. esculentum against P. aeruginosa and S. epidermidis. The most potent antibacterial activity was observed at 80 and 60% concentrations against P. aeruginosa, resulting in inhibition zones with 5.90 mm and 5.47 mm diameters. A similar pattern was also observed against S. epidermidis, where 80% concentration showed significant efficacy, with an inhibition zone of 5.69 mm diameter. Phytochemical analysis result revealed the presence of various secondary metabolites, including alkaloids, steroids, polyphenols, tannins, flavonoids, and saponins. These phytochemical compounds contribute antibacterial properties and have promising potential for further research to develop natural antibacterial agents.
Antibacterial activity and compound identification of Eurya acuminata leaf fractions against bacteria-causing skin infections FESKAHARNY ALAMSJAH; ANTHONI AGUSTIEN; ARTHAULY YOPITA SINURAT; MUFIDHATUL MUQARRAMAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 8 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250815

Abstract

Abstract. Alamsjah F, Agustien A, Sinurat AY, Muqarramah M. 2024. Antibacterial activity and compound identification of Eurya acuminata leaf fractions against bacteria-causing skin infections. Biodiversitas 25: 3441-3448. Antibiotics previously used to treat skin infections have recently been ineffective due to pathogenic bacteria resistance, and the discovery of antibacterial agents derived from medicinal plants is a breakthrough. Eurya acuminata DC. is a member of the Pentaphylacaceae family and has efficacy values as a medicinal plant used in traditional medicines. This study aims to analyze the antibacterial activity of fractions (hexane, ethyl acetate, and water) of E. acuminata against bacteria causing skin infections and identify the chemical components of active fractions. A diffusion test was conducted for the antibacterial test. TLC (Thin Layer Chromatography) was used to determine the chemical components of the fractions. The fraction with the highest level of antibacterial activity was subjected to additional analysis using diffusion and LC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry). The antibacterial test showed that hexane, ethyl acetate, and water fractions were active against bacteria, with the ethyl acetate fraction having the highest activity. The TLC profile and the diffusion assay of the ethyl acetate fraction showed that spot number 4 with an Rf 1,00 had antibacterial activity. Identification of the compounds of the ethyl acetate fraction by LC-MS/MS resulted in identified compounds such as acacetin, kaempferol, peonidin, chrysoeriol, nobiletin, luteolin, and antroquinonol. This study demonstrates a new hope for further research into natural antibacterial agents, and it is a breakthrough with these phytochemicals.
Optimization and molecular identification of PUA-14 bacterial isolate from protease-producing mangrove waters Yorasakhi Ananta; Feskaharny Alamsjah; Anthoni Agustien
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260120

Abstract

Abstract. Ananta Y, Alamsjah F, Agustien A. 2025. Optimization and molecular identification of PUA-14 bacterial isolate from protease-producing mangrove waters. Biodiversitas 26: 190-198. Protease enzymes, the hydrolase enzymes that break down proteins into simpler molecules, are a vital component of the food, pharmaceutical, and other chemical industries. By selecting microorganisms as enzyme producers, we can meet the high demand for enzymes and promote sustainable production. This study aimed to determine the optimum conditions for protease production by protease-producing PUA-14 bacterial isolates from mangrove waters and to identify the isolates through biomolecular analysis. We conducted laboratory experiments to optimize protease production by varying carbon sources, nitrogen sources, trace elements, and inoculum concentrations. The optimization process employed the One Factor at A Time (OFAT) approach and the Central Composite Design (CCD) within the framework of Response Surface Methodology (RSM) using Design Expert 13 software. Furthermore, biomolecular analyses, including DNA isolation, amplification, and sequencing, were performed to identify the bacterial isolates. The study identified the optimum conditions for protease production by the PUA-14 bacterial isolate are 0.5% lactose, 2.5% NaNO3, Zn as the trace element, and 2.5% inoculum concentration. Molecular analysis confirms the similarity of the PUA-14 bacterial isolate to Bacillus pseudomycoides strain LB-AsDX1-3. These research findings not only provide a foundation for future research but also have immediate practical implications, highlight the potential for sustainable enzyme production under optimized conditions, and support a wide range of industrial applications.
Structure and composition of understory vegetation in urban forest of Pekanbaru, Riau Province, Indonesia DARMAWATI DARMAWATI; LIDIA BELINDA SILITONGA; NURSAL NURSAL; IRDA SAYUTI; IMAM MAHADI; FESKAHARNY ALAMSJAH
Biodiversitas Journal of Biological Diversity Vol. 26 No. 9 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260933

Abstract

Abstract. Darmawati, Silitonga LB, Nursal, Sayuti I, Mahadi I, Alamsjah F. 2025. Structure and composition of understory vegetation in urban forest of Pekanbaru, Riau Province, Indonesia. Biodiversitas 26: 4628-4639. Urban forests play a crucial role in maintaining biodiversity; however, human disturbances and the spread of invasive species pose significant challenges to the resilience of understory vegetation. This study examined the structure and composition of understory vegetation in the Pekanbaru Urban Forest in Riau Province, Indonesia and formulated strategies for sustainable management. Research was carried out from June to December 2024 across two canopy conditions Closed Canopy Forest (CCF) and Partial Canopy Forest (PCF) using ten 2×2 m plots in each site (80 m² total). The analyses encompassed species composition, Shannon-Wiener Diversity Index (H?), Evenness Index (E), Dominance Index (D), invasive species proportion, correlation between H? and light intensity, as well as a T-test to compare H? between canopy types. Results revealed that the Poaceae family was the most dominant, with Brachiaria decumbens as the representative species. The T-test showed no significant difference in H? between CCF (2.67) and PCF (2.98), both classified as moderate. Evenness Index values were high (0.89 and 0.87), while dominance values were very low (0.08 and 0.07), indicating a relatively even distribution of species. In total, 38 species were identified, half of which (19 species) were invasive, with a higher abundance recorded in PCF. These findings suggest that canopy mosaics comprising both closed and partially open areas are essential for maintaining understory vegetation in urban forests, as they help support species diversity while limiting invasive dominance. Recommended management strategies include preserving canopy mosaics, controlling invasive species, promoting native species planting, and strengthening conservation policies through active community participation.
PENAPISAN DAN KARAKTERISASI BAKTERI TERMOFILIK DARI SUMBER AIR PANAS SAPAN, KABUPATEN SOLOK SELATAN SEBAGAI PENGHASIL ENZIM TERMOSTABIL PROTEASE Muqarramah, Mufidhatul; Agustien, Anthoni; Alamsjah, Feskaharny
Biospecies Vol. 19 No. 2 (2026): Juli 2026
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v19i2.48949

Abstract

 Thermostable protease enzymes can be produced by thermophilic bacteria that act as biocatalysts in protein hydrolysis and have extensive industrial applications. The supply of stable and high-performance protease enzymes is still insufficient, encouraging exploration of thermophilic bacteria from extreme environments as potential producers. The aim of this study was to identify and characterize the macroscopic, microscopic, physiological, and biochemical features of thermophilic bacterial isolates that indicated protease production. This study was conducted using descriptive methods through rejuvenation, screening, and characterization of thermophilic bacteria isolated from the Sapan Hot Spring, South Solok Regency, as potential thermostable protease producers. Based on the results, six protease-producing thermophilic bacterial isolates, namely TUA-01, TUA-02, TUA-11, TUA-21, TUA-26, and TUA-31, with the highest Proteolytic Index (PI) value being isolate TUA-26. Characterization of the six protease-producing thermophilic bacterial isolates revealed differences in colony morphology characteristics, Gram-positive, rod-shaped cells, subterminal spores, motility, and catalase-positive. The thermophilic bacterial isolate TUA-26 has the potential to produce thermostable protease enzymes at high temperatures, making it highly promising for use in various biotechnology and industrial applications.
PEMBERDAYAAN IBU RUMAH TANGGA DALAM PEMANFAATAN MICROGREEN DAN HEALTHY JUICE UNTUK GIZI KELUARGA Mairawita; Zozy Aneloi Noli; Feskaharny Alamsjah; Solfiyeni; Mildawati
Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Vol. 3 No. 2 (2024): Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jai.v3i2.742

Abstract

Microgreens and healthy juice are closely related to family nutrition and a healthy lifestyle. This community service activity aims to empower homemakers to use microgreen vegetables in the form of kale and kale and make healthy juice from these vegetables to fulfill family nutrition needs as part of efforts to live a healthy lifestyle. Community service activities regarding the introduction of microgreens and healthy juices were held on June 13, 2024, in Limau Manis Selatan Village, Unand Block B lecturer housing complex, Ulugadut, Pauh District, Padang, which was attended by 50 members of Majlis Taklim Darul Ulum. Implementation methods include socialization, discussion, and direct practice. As many as 90% of participants showed increased knowledge about microgreens, and 85% better understood the importance of healthy juice and how to make it. Awareness regarding participants' nutritional knowledge increased, with greater interest in including microgreens and healthy juices in their daily diet. Activities achieved satisfactory results as demonstrated by high participation and enthusiasm during socialization, discussion, and practice sessions. At the end of the activity, interviews were conducted with participants. Live demonstrations and independent practice are proven effective in teaching new knowledge and skills. Challenges such as time and material limitations were overcome with a follow-up plan, including forming small working groups and follow-up sessions to evaluate progress. This activity increased the knowledge, skills, and enthusiasm of Majlis Taklim Darul Ulum members to live a healthy lifestyle. Post-training, the results show the potential for sustainability in encouraging healthy lifestyles of training participants and strengthening communities.
Co-Authors Alivia Zulkarnain Alivia Zulkarnain Anisa Rahman Rusiati Anisa Rahman Rusiati Anisa Rahman Rusiati Anthoni Agustien Apriliana Wahyuni Arthauly Yopita Sinurat Ayumi Rizci Puspita Ayumi Rizci Puspita Ayumi Rizci Puspita DARMAWATI DARMAWATI Dean Bilalwa Agusto Djong Hon Tjong Fadhila Cantika Fajar Irianto Fajar Irianto Firman Ridwan Hesti Dwi Marcellinna Ikel Fitri Yanis Ilham, Kurniadi IMAM MAHADI Indra Laksmana IRDA SAYUTI Kawther Ameen Muhammed Saeed Aledresi Kurniadi Ilham LIDIA BELINDA SILITONGA Mairawita Mairawita, Mairawita Mellanie Alia Putri Mellanie Alia Putri Mildawati Mildawati Mildawati Mohsin Rafique Mufidhatul Muqarramah Muhammad Restu Raimon NURSAL NURSAL Puspita, Ayumi Rizci Rafia Rafia Rafia Rafia Rahmayati, Riesca Salsabilah Riesca Salsabilah Rahmayati Riska Riska Riska Riski Budi Yani Rusydi, Muhammad Ilhamdi SASQIA FANDINI Solfiyeni Solfiyeni Suwirmen, Suwirmen Tesri Maideliza Tri Widia Ningsih Alam Wismalqi Wismalqi Yetti Marlida Yorasakhi Ananta Zil Fadhillah Rahma Zozy Aneloi Noli Zozy Aneloi Noli