Enny Randriani
Balai Penelitian Tanaman Rempah dan Aneka Tanaman Industri, Jl. Raya Parungkuda Km. 2 Parungkuda, Sukabumi 43357 Telp. (0266) 531241

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Seleksi Genotipe Unggul Kopi Robusta Spesifik Lokasi Dani, Dani; Tresniawati, Cici; Randriani, Enny
Jurnal Tanaman Industri dan Penyegar Vol 4, No 2 (2013): Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

Seleksi genotipe unggul kopi Robusta yang memiliki karakteristik biji besar, kandungan kafein rendah, dan citarasa baik sangat penting dilakukan dalam rangka meningkatkan nilai ekonomi kopi Robusta di pasar global. Tujuan penelitian ini adalah untuk melakukan seleksi genotipe kopi Robusta terbaik berdasarkan kriteria-kriteria tersebut di atas. Waktu pelaksanaan penelitian pada bulan Januari-Desember 2012. Bahan seleksi berupa populasi lima genotipe kopi Robusta hasil seleksi petani (MCJ-1, SCJ-1, PKCJ-1, PHCJ-1, dan SuCJ-1) yang telah banyak dibudidayakan di wilayah Kabupaten Curup, Provinsi Bengkulu. Kriteria seleksi berdasarkan karakteristik mutu fisik dan morfometrik biji beras, kandungan kafein, dan mutu citarasa seduhan. Pengujian mutu fisik biji beras, kandungan kafein, dan mutu citarasa seduhan dilaksanakan di Pusat Penelitian Kopi dan Kakao Indonesia, Jember. Berdasarkan hasil pengujian diketahui bahwa genotipe PKCJ-1 memiliki karakteristik ukuran biji paling besar dan kandungan kafein paling rendah. Genotipe PHCJ-1 dan SCJ-1 paling baik dalam hal citarasa dan telah memenuhi kategori salah satu kriteria kopi spesialti. Dengan demikian, berdasarkan kriteria seleksi yang telah ditetapkan, ketiganya terpilih sebagai genotipe harapan.Kata Kunci: Kopi Robusta, seleksi genotipe, kafein rendah, mutu citarasaSelection of Robusta coffee genotypes which have superior characteristics, such as large beans size, low caffeine content, and good cup quality taste, is essential in order to increase its economic value in the world market. The objectives of the research was to select the superior genotype(s) of Robusta coffee based on characteristics as mentioned above. The research was carried out at Januari to December 2012. Material used was five genotypes of farmer-selected Robusta coffee (MCJ-1, SCJ-1, PKCJ-1, PHCJ-1, and SuCJ-1) recently grown in many areas across Curup Regency, Bengkulu Province. Selection criteria was physical quality and morphometric characteristics of green beans, caffeine content, and cup quality. The laboratory test was conducted at the Center for Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember. The results showed that the genotype of PKCJ-1 has the largest in size of bean and the lowest in caffeine content. On the other hand, PHCJ-1 and SCJ-1 genotypes are the best in terms of taste and meets of ones criteria for specialty coffee grade. Thus, these three genotypes were selected as a candidate of superior genotypes.
KEKERABATAN PLASMA NUTFAH JAMBU METE BERDASARKAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA Randriani, Enny; Listyati, Dewi; ., Syafaruddin
Jurnal Tanaman Industri dan Penyegar Vol 2, No 2 (2011): Buletin Riset Tanaman Rempah Dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Genetic Relationship of Cashew Germplasm Based on Random Amplified Polymorphic DNA. Cashew production in Indonesia stilllow (300-463 kg/ha/year) caused by genetic materials used for the cultivation were not superior variety, therefore, some effort to find superiorvariety with high production must be improved. To support those efforts needs wide variation of plant genetic resources. Nowd ays, characteristic dataof cashew germplasm in BALITTRI based on morphological characters, so needed molecular approach to get genetic characteristic s, geneticrelationship and specific characters such as high production marker, one of some techniques that we can use is RAPD marker. Additionally, RAPDmarker is simple, efficient and accurate. The purpose of this study was to know the genetic variation and relationship among cashew germplasm basedon band pattern of DNA by using RAPD technique. The experiment was conducted at Molecular Biology Laboratory of BB-Biogen, Bogor since Maytill November 2009. Genetic material used were MR 851, PK 36, GG1, Laode Kase, Laode Kapala, JT 27, Arsyad Labone, Wonogiri Merah, A x S,F x M, SM 9, C x M, F x A and BO2 by using 25 primers. The activity consisting germplasm collecting of cashew (14 accessions), followed laboratoryactivities such as: DNA extraction and purification, loading and running of PCR product, RAPD and data analysis. Results shows that 25 primersused are 16 primers shown DNA band pattern, one of them was monomorphism and one specific primer which shown DNA band pattern of cashew,i.e: BO2, SM9 and JT27. Germplasm collection of cashew has wide variation. At 70% coefficient, 14 accessions of cashew were divided to threegroups where first group were content 11 individual (MR 851, PK 36, Laode Kase, GG1, Laode Kapala, A x S, F x A, C x M, Arsyad Labone,Wonogiri Merah, and F x M), while second group were content two individuals (BO2 and SM9). Moreover, in first group itself sh own wide variationamong 11 accessions.
Identifikasi Karakter yang Berpengaruh terhadap Hasil Biji Beras Kopi Arabika di Kabupaten Garut Menggunakan Analisis Lintasan Bertahap Randriani, Enny; Dani, Dani; Sulistiyorini, Indah; Wardiana, Edi
Jurnal Tanaman Industri dan Penyegar Vol 1, No 3 (2014): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Keterkaitan antara karakter vegetatif, komponen buah, komponen biji, dan hasil biji beras pada tanaman kopi merupakan hal penting dalam program pemuliaan dan seleksi. Model saling keterkaitan antar karakter tersebut adalah model sebab-akibat dan dapat dianalisis melalui analisis lintasan bertahap (ALB). Penelitian bertujuan mengidentifikasi karakter-karakter yang berpengaruh terhadap hasil biji beras kopi Arabika di daerah Garut, Jawa Barat melalui penggunaan analisis lintasan bertahap (ALB) dan model persamaan struktural (MPS). Penelitian dilaksanakan di Desa Marga Mulya, Kecamatan Cikajang, Kabupaten Garut, Jawa Barat, bulan Januari sampai Desember 2013 pada ketinggian tempat 1.300 m di atas permukaan laut dengan jenis tanah Andosol. Penelitian menggunakan metode survei dengan pengambilan contoh secara acak terhadap lima genotipe kopi Arabika, yaitu ABP-1, ABP-2, ABP-3, AGK-1, dan S 795. Hasil penelitian menunjukkan terdapat tiga karakter yang berpengaruh positif secara langsung, yaitu jumlah ruas pada batang, tebal buah, dan bobot 100 biji gabah. Karakter panjang cabang primer berpengaruh positif secara tidak langsung, sedangkan karakter jumlah cabang sekunder dan jumlah ruas cabang primer berpengaruh negatif secara tidak langsung. Karakter-karakter tersebut dapat digunakan sebagai kriteria seleksi pada populasi kopi Arabika di daerah Garut, Jawa Barat.Kata kunci: Kopi Arabika, seleksi karakter, analisis lintasan bertahap, model persamaan strukturalThe linkage between the vegetative characters, fruit components, production seeds component and rice seeds of the coffee plant is important in breeding and selection programs. Models of interrelations between these characters are causal models and can be analyzed through sequential path analysis (SPA). The objective of this study was to identify of several characters affecting on green bean yield of Arabica coffee in Garut, West Java by using sequential path analysis (SPA) and structural equation modeling (SEM). This research was conducted in the Marga Mulya Village, Cikajang District, Garut Regency, West Java, with altitude about 1300 m above sea level and Andosol type of soil, starting from January to December 2013. The research was conducted in survey method with random sampling method on the Arabica coffee genotypes such as ABP-1, ABP-2, ABP-3, AGK-1, and S795. The results showed that there are three characters having directly positive effect on green bean yield: number of internodes on stem, fruits thickness, and weight of 100 beans. On the other hand, length of primary branches has indirectly positive influence, while number of secondary branches and number of internodes on primary branches has indirectly negative influence. These characters can be used as selection criteria on the population of Arabica coffee in Garut, West Java. 
Variasi Genetik dalam Populasi Kultivar Kopi Arabika Berbuah Kuning di Lahan Petani Berdasarkan Penanda SSRs Dani, Dani; Izzah, Nur Kholilatul; Randriani, Enny
Jurnal Tanaman Industri dan Penyegar Vol 3, No 2 (2016): Jurnal Tanaman Industri dan Penyegar
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v3n2.2016.p83-94

Abstract

Identification of the genetic diversity within populations of yellow-berried Arabica coffee cultivar based on morphological characters faced an obstacle in finding identical environmental conditions at farmers field. Therefore, an approach which is not influenced by differences in environmental conditions is required, for instance based on DNA polymorphism. The research aimed to analyze genetic variation within populations of yellow-berried Arabica coffee cultivar based on SSRs markers. The research was conducted in the Integrated Laboratory, Indonesian Industrial and Beverage Crops Research Institute, Sukabumi, from April until June 2015. The leaf samples for DNA extraction were obtained from yellow-berried Arabica coffee cultivar (AGK-1) and two red-berried cultivars  as controls, namely  ABP-1 (dwarf type) and Typica (tall type). AGK-1 and ABP-1 cultivars consisted of 17 and 5 individual numbers, respectively, whereas Typica cultivar comprised three individuals. PCR amplification was carried out using 12 SSR primers. Four primers (M24, SSRCa052, M32, and M42) produced polymorphic band. The binary data obtained in this research was subsequently processed using NTSYS-PC program version 2.1. The genotypes were grouped  based on a genetic similarity matrix using the unweighted pair group method arithmetic mean (UPGMA). The result showed the existence of genetic variation among individual of AGK-1 cultivars, which forming three clusters at the genetic similarity value of 67%. One cluster exhibited close genetic relationships between some individuals within the population of AGK-1 cultivar and Typica cultivar. Meanwhile, the other two clusters showed high genetic similarity between AGK-1 cultivar and ABP-1 cultivar. The result demonstrated the possibility of gene flow between genotypes or residual heterozygosity within the population of  AGK-1 cultivar at farmers field, which required a further study.