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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Tropical and Infectious Disease MES: Journal of Mathematics Education and Science Jurnal Layanan Masyarakat (Journal of Public Service) JURNAL CURERE Journal of Community Service Journal of Education Technology and Inovation
Ratna Wahyuni
eprosy Study Group, Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara Dinar Adriaty; Ratna Wahyuni; Iswahyudi Iswahyudi; Bimo Aksono; Toetik Koesbardiati; Indropo Agusni; Shinzo Izumi
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.2.3.%x

Abstract

The existence of leprosy or kusta or Morbus Hansen or Hansens disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms).
Co-Authors Abdullah, Salsabilla Putri Kinanti Amalia Ajrina Bimo Aksono Cita Rosita Sigit Prakoeswa Deby Julianda Reulina Sitepu DINAR ADRIATY Eti Muliani Indah Simamora INDROPO AGUSNI Iswahyudi Iswahyudi Iswahyudi Laura Navika Yamani M. Rudy Sumiharsono Medhi Denisa Alinda, Medhi Denisa Muljono Muljono Puput Ade Wahyuningtyas Shinzo Izumi Simbolon, Novi Tari Syamsiar, Syamsiar Taufiqurrahman Sidqi Toetik Koesbardiati Yessica Sinabariba