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All Journal Jurnal Ilmu Ternak Veteriner Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal ANGON: Journal of Animal Science and Technology Jurnal Agripet Animal Production
Efka Aris Rimbawanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Veterinary

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ISOLASI DAN EKSTRAKSI DAUN KALIANDRA (Calliandra calothyrsus) SEBAGAI KONSENTRAT PROTEIN DAUN DITINJAU DARI TITIK ISOELEKTRIK, ASAM AMINO DAN ZAT ANTINUTRISI Wahyudi, Imam; Rimbawanto, Efka Aris
ANGON: Journal of Animal Science and Technology Vol 4 No 2 (2022): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2022.4.2.p204-212

Abstract

Background. Research "Isolation and Extraction of Calliandra (Calliandra calothyrsus) Leaf as Leaf Protein Concentrate Judging from Isoelectric Point, Amino Acids and Antinutrients". This study aims to determine the isoelectric point, total amino acid content and the content of antinutrient tannins in the protein concentrate of calliandra leaves extracted and isolated from the protein. Materials and Methods. The research material used was calliandra (Calliandra calothyrsus) leaves. Determination of the isoelectric point using the modified Yatno method (2009), the total amino acid content using the high-performance liquid chromatography (HPLC) method, crude protein analysis using the Kjedhal according to AOAC (2005), and the content of antinutrient tannins using the butanol-HCl method. Results. The results showed that the isoelectric point of the calliandra leaf protein concentrate was reached in the first extraction with 0.05 N NaOH and pH 8 phosphate buffer in the second extraction resulting in 7.2 mg/ml protein precipitate with a crude protein content of 48.94%. The amino acid composition of calliandra leaf protein concentrate was higher than that of soybean meal except for glutamine. Conclusion. The tannin content produced in this study was 0.01% so that the calliandra leaf protein concentrate was safe to use as a protein source in poultry rations.
PENGGUNAAN ASAM LAKTAT SEBAGAI FEED ADITIF TERHADAP AKTIVITAS SGPT & SGOT AYAM SENTUL Prihambodo, Tri Rachmanto; Hartoyo, Bambang; Rimbawanto, Efka Aris
ANGON: Journal of Animal Science and Technology Vol 4 No 3 (2022): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (912.754 KB) | DOI: 10.20884/1.angon.2022.4.3.p291-296

Abstract

Background. The large number of species spread across Indonesia cannot be separated from the domestication process which helps improve and maintain livestock species by humans to maintain food needs for animals such as the domestication of the Sentul chicken. Poor feed efficiency for Sentul chickens has caused Sentul chickens to depend on antibiotics and has been banned since 2018. Lactic acid is believed to be an alternative to antibiotics and has been studied for a long time. The liver is an organ that can represent the state of the chicken if lactic acid is identified as a hazardous substance through the content of SGPT and SGOT. This study aims to evaluate the use of lactic acid as an acidifier in sentul chickens found in the liver seen from the content of SGOT and SGPT. Materials and Methods. There were 60 Sentul chickens used in this study which measured the content of SGOT and SGPT. Results. The addition of lactic acid significantly (p>0.05) did not have a negative effect on the liver in both SGOT and SGPT at administration levels up to 2% (v/g). SGOT and SGPT in the negative control were 232.9 and 39.68 mmol/L while the addition of lactic acid was still in a range that was not too far away, namely for SGOT in the range of 234.6-281.2 mmol/L and SGPT 35.15-56.96 mmol/L. Conclusion. The use of lactic acid in Sentul chicken feed does not cause liver damage which can be seen in SGOT and SGPT levels.
ISOLASI DAN EKSTRAKSI DAUN Indigofera zollingeriana SEBAGAI KONSENTRAT TEPUNG DAUN BERDASARKAN TITIK ISOELEKTRIK, ASAM AMINO DAN ZAT ANTINUTRISI Prasetyo, Anugrah Eko; Rimbawanto, Efka Aris; Hartoyo, Bambang
ANGON: Journal of Animal Science and Technology Vol 5 No 1 (2023): ANGON: Journal of Animal Science and Technology
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.angon.2023.5.1.p68-77

Abstract

Salah satu bahan pakan dengan sumber protein tinggi adalah bungkil kedelai. Namun, saat ini bahan pakan tersebut masih di impor dari luar negeri, yang menyebabkan bungkil kedelai memiliki harga yang relatif tinggi dan jumlahnya terbatas. Pengurangan ketergantungan akan bahan pakan bungkil kedelai dilakukan dengan mengeksplorasi bahan pakan lokal dengan keteenrsediaan jumlah yang memadai dan memiliki kandungan nutrient yang tinggi. Tujuan dari penelitian ini adalah untuk mengetahui titik isoelektrik, kandungan asam amino total dan kandungan zat antinutrisi tanin yang tepat pada pembuatan konsentrat protein daun Indigofera zollingeriana hasil dari ekstrasi dan isolasi protein. Tahapan ekstrasi dilakukan 2 tahap, NaOH 0,05 N pH 8 pada ekstrasi tahap pertama dan buffer asetat pH 4 pada tahap kedua. Penentuan isoelektrik, menurut menggunakan metode Yatno (2009) modifikasi high-performance liquid chromatography (HPLC) untuk kandungan asam amino total. Analisis protein kasar, menurut AOAC (2005) digunakan metode kjedhal, dan metode butanol-HCl digunakan pada uji kandungan zat antinutrisi tanin. Penelitian ini menghasilkan titik isoelektrik konsentrat protein pada daun Indigofera zollingeriana tercapai pada NaOH 0,05 N pH 8 pada ekstraksi pertama dan buffer fosfat pH 8 pada ekstraksi kedua menghasilkan endapan 0,108 g/ gramBKdaun dengan kandungan protein kasar sebesar 65,98% (lebih tinggi dari bungkil kedelai) serta kandungan asam amino yang lebih lengkap dari bungkil kedelai dan tidak memiliki kandungan tanin.
Co-Authors Agung Prastyo Nugroho Agung Prastyo Nugroho Ajat Sudrajat Albasheer, Modawy Abdelgader Apit Mulyana Bambang Hartoyo Budi Rustomo Caribu Hadi Prayitno Dadang Mulyadi Saleh Endang Baliarti Fransisca Maria Suhartati Hakim, Aulia Rizaldi Hafiz Ifani, Merryafinola Imam Wahyudi Indrawan, Imam Wahyudi Ismoyowati Iswanto, Dimas Oqi Nur Kasihandari, Yut Afiffah Lies Mira Yusiati Muhamad Bata Ning Iriyanti Ning Iriyanti Ning Iriyanti Pajriyah, Wafiq Siti Nur Prasetyo, Anugrah Eko Prayitno Prayitno Raden Febrianto Christi Refriana, Rosita Ristianto Utomo S.N.O. Suwandyastuti Safitri, Afifah Dian Sahawaludin, Risqi Saputro, Alif Rizki Tixko Sri Rahayu SRI RAHAYU Sri Suhermiyati Suhartati, F M Suhartati, F. M. Tri Rachmanto Prihambodo Triana Yuni Astuti Wardhana Suryapratama