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All Journal HAYATI Journal of Biosciences CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Jurnal Veteriner Ecotrophic, Journal of Environmental Science Indonesian Journal of Cancer Jurnal Media Sains Journal of Health Sciences and Medicine INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH Indonesian Journal of Cancer
WIWIK SUSANAH RITA
Chemistry Department, Faculty Of Mathematic And Natural Sciences Universitas Udayana Badung, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Media Sains

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Aktivitas Minyak Atsiri Daun Cengkeh Sebagai Antijamur Terhadap Candida albicans Mbatu, Rosanti Suryani Tince; Kenanda, I Putu Bayu; Suharta, I Gede Yeyen; Rita, Wiwik Susanah
Jurnal Media Sains Vol 2, No 1 (2018): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.085 KB) | DOI: 10.36002/jms 3.v2i1.360

Abstract

ABSTRAKSalah satu jamur patogen yang dapat menyebabkan masalah kesehatan yaitu Candida albicans. Untuk menghambat pertumbuhan jamur dapat memanfaatkan bahan aktif dari tanaman yakni minyak atsiri daun cengkeh sebagai bahan antijamur. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur dan daya hambat minyak atsiri daun cengkeh terhadap jamur Candida albicans dan konsentrasi terendah minyak atsiri daun cengkeh yang mampu menghambat pertumbuhan jamur Candida albicans. Ekstraksi minyak atsiri daun cengkeh dilakukan dengan metode destilasi uap, menghasilkan rendemen sebesar 1,525%. Uji aktivitas antijamur dilakukan menggunakan metode sumur difusi dengan media Potato Dextrose Agar (PDA) menghasilkan diameter zona hambat sebesar 15 mm, menunjukkan minyak atsiri daun cengkeh memiliki daya hambat kuat terhadap pertumbuhan jamur Candida albicans. Uji daya hambat minyak atsiri daun cengkeh terhadap jamur Candida albicans menunjukkan perbedaan nyata pada konsentrasi 0,5% sampai 10%, dan konsentrasi terendah minyak atsiri daun cengkeh dapat menghambat pertumbuhan jamur Candida albicans adalah pada konsentrasi 0,5% (v/v) dengan diameter zona hambat sebesar 5,67 mm.Kata kunci : minyak atsiri, daun cengkeh, Candida albicans, antijamurABSTRACTOne of the pathogenic fungi that can cause various health problems is Candida albicans. To inhibit the growth of fungi by use the active compound of the plants which is clove essential oil that have antifungi activity. This research aims to determine the antifungal activity, inhibitory power of clove leaf essential oil and lowest concentration of clove leaf essential oil that can inhibit the growth of Candida albicans. The clove leaf essential oil extraction was done by steam distillation method, the rendement is 1,525%. The antifungal activity test was performed using diffusion well method with Potato Dextrose Agar (PDA) medium yielding 15 mm inhibition zone diameter, showing clove leaf essential oil having strong inhibition effect on Candida albicans fungus growth. Clove oil essential oil inhibition test to Candida albicans fungi showed significant difference at 0.5% to 10% concentration, and the lowest concentration of clove leaf essential oil could inhibit the growth of Candida albicans fungus at 0.5% (v / v) concentration with inhibition?s zone of 5.67 mm. Key words: Essential oil,Clove leaf, Candida albicans, antifungi
Potensi Ekstrak Etanol Daun Kelor (Moringa oleifera) Terhadap Penurunan Kadar Histamin Pada Ikan Lemuru (Sardinella longiceps) Valent, Febryna Aurora; Oka Adi Parwata, I Made; Susanah Rita, Wiwik
Jurnal Media Sains Vol 1, No 2 (2017): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.082 KB) | DOI: 10.36002/jms 3.v1i2.262

Abstract

ABSTRAK ?Tanaman kelor merupakan suku Moringaceae yang telah menjadi obyek pada banyakpenelitian karena mengandung senyawa aktif seperti flavonoid, saponin, tanin, dan senyawafenolik lain yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahuipotensi ekstrak etanol daun kelor (Moringa oleifera) terhadap penurunan kadar histamin padaikan lemuru (Sardinella longiceps). Penelitian ini juga bertujuan untuk mengetahui konsentrasi minimum ekstrak etanol daun kelor dan penentuan kadar total flavonoid yang terdapat di dalamekstak etanol daun kelor. Variasi konsentrasi ekstrak etanol yang digunakan adalah 0%, 7%,8%, 9%, 10%, dan 15% dengan lama perendaman selama 0, 30, 60, dan 90 menit. Kadarhistamin diuji dengan alat Biofish dan hasilnya dianalisis dengan menggunakan Two WayANOVA untuk mengetahui perbedaan yang berarti sebagai respon terhadap perlakuan. Hasilpenelitian menunjukkan bahwa variasi konsentrasi ekstrak dan lama perendaman memilikiperbedaan yang nyata (P<0,05) dan konsentrasi minimum ekstrak yang dapat menurunkan kadar histamin adalah 8%. Kadar total flavonoid pada ekstrak etanol daun kelor yang digunakanadalah 3177,11 mg/100 g QE.Kata kunci: Sardinella longiceps, Moringa oleifera, histamin, flavonoidABSTRACT Moringa plants are Moringaceae tribes that have been the object of many studiesbecause they contain active compounds such as flavonoids, saponins, tannins, and other potentially antibacterial phenolic compounds. The aim of this research is to know the potency ofmoringa leaf ethanol extract (Moringa oleifera) to decrease histamine levels in lemuru fish(Sardinella longiceps). This study also aims to determine the minimum concentration ofmoringa leaf ethanol extract and the determination of total flavonoid content c ontained inethanol extract of moringa leaf. Variations of ethanol extract concentration used were 0%, 7%,8%, 9%, 10%, and 15% with soaking time for 0, 30, 60, and 90 min. Histamine levels weretested with a Biofish tool and the results were analyzed using Two Way ANOVA to determinethe significant differences in response to the treatment. The results showed that v ariation of extract concentration and soaking time had significant difference (P<0,05) and the minimumconcentration of extract which could decrease histamine level was 8%. The total flavonoidcontent of moringa leaf? ethanol extract used was 3177.11 mg/100 g QE.Keywords: Sardinella longiceps, Moringa oleifera, histamine, flavonoids
Co-Authors Abdul Rahim Adi Djona Silaen Ali Sabikin Frida R. Koreh Gede Widihati, Ida Ayu HERNINDYA, ANISA I Gusti Agung Gede Bawa I Gusti Ayu Kunti Sri Panca Dewi I Gusti Bagus Teguh Ananta I Kadek Pater Suteja I M, Sukadana I Made Adi Suardhyana I Made Dira Swantara I Made Oka Adi Parwata I Made Siaka I Nyoman Suarsana I Nyoman Suartha I W BUDIARSA SUYASA I Wayan Gede Gunawan I Wayan Suirta I. A. R. Astiti Asih Irdhawati Irdhawati Irvansyah Habibi Resaputra Iryanti Eka Suprihatin James Sibarani Kenanda, I Putu Bayu Mbatu, Rosanti Suryani Tince Ni Kadek Dyan Mustika Sri Wahyuni Ni Ketut Sinarsih Ni Ketut Sinarsih Ni Komang Ariati Ni Luh Putu Lilis Wirastiningsih Ni Luh Rustini Ni Luh Yuli Damayanti Ni Made Dwita Prasetyawati Ni Made Puspawati Ni Made Puspawati Ni Made Suaniti Ni Made Sukma Sanjiwani Ni Made Yuliari Ni Putu Eka Vinapriliani Ni Putu Widayanti Putu Prisanti Putri Utami Putu Puspita Sari Putu Sariningsih Raditya Yoga Rahayu, Riana Suastari Retno Kawuri Rr Anisa Hernindy Sinarsih, Ni Ketut Suharta, I Gede Yeyen Valent, Febryna Aurora WAHYU DWIDJANI S Wirajana, Nengah Yenni Ciawi Yohanes Armawan Sandi