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All Journal Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medical Journal of Indonesia Dental Journal (Majalah Kedokteran Gigi) Universa Medicina Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Biomedika dan Kesehatan

Yeva Rosana

Department Of Microbiology, Faculty Of Medicine, Universitas Indonesia, Jakarta 10430

Author-ID : 364169

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Public Health

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Implementation of 25-well culture plates for M. tuberculosis drug susceptibility testing in Indonesia Rosana, Yeva; Sudiro, Tjahjani M.; Alisjahbana, Bachti; Parwati, Ida; van Crevel, Reinout; van Soolingen, Dick
Medical Journal of Indonesia Vol 14, No 3 (2005): July-September
Publisher : Faculty of Medicine Universitas Indonesia

At present, there is no standardized method for Mycobacterium tuberculosis drug susceptibility testing (DST) among laboratories in Indonesia. Since January 2001 to January 2004 we have tried to establish the method of 25-well culture plates with middlebrookâs media (Drug Susceptibility Culture Plate (DSCP) method) used by the Dutch Supranational Reference Laboratory at the Institute of Public Health and the Environment (RIVM), Bilthoven, Netherlands. Our experience showed that this method potentially gives better result as it can be very well standardized, faster and provides detailed MIC (Minimal Inhibitory Concentration) values. Data from 364 isolates that have been tested by DSCP method showed that resistance to INH, rifampicin, ethambutol, and streptomycin were 21.4%, 19.8%, 15.7%, and 16.5% respectively. Multidrug resistance were found in 13.2% isolates. (Med J Indones 2005; 14: 142-6)Keywords: M. tuberculosis, DST, DSCP method

Patterns of bacterial resistance against Ceftriaxone from 2002 to 2005 in the Clinical Microbiology Laboratory of the Faculty of Medicine, University of Indonesia Rosana, Yeva; Kiranasari, Ariyani; Ningsih, Ika; Tjampakasari, Conny; Kadarsih, Retno; Wahid, Mardiastuti H.
Medical Journal of Indonesia Vol 16, No 1 (2007): January-March
Publisher : Faculty of Medicine Universitas Indonesia

The spread of drug resistant microbes is a global public health challenge which impairs the efficacy of antimicrobial agents and causes substantial increase in morbidity and mortality rates, including healthcare-associated costs. Monitoring of antimicrobial drug resistance from documented microbial epidemiology & resistance rate is useful in preventing the emergence of resistance. This study reports on the pattern of bacterial resistance against ceftriaxone in the past 4 years. The data were obtained from specimens examined in the Clinical Microbiology Laboratory, Department of Microbiology Faculty of Medicine, University of Indonesia from 2002 to 2005. Microbial species were determined from culture and identification tests. Disc diffusion method was used for sensitivity testing of ceftriaxone to 14 Gram-negative and 7 Gram-positive bacteria. Although resistance rates were increased from 2002 to 2005, resistance rates of ceftriaxone were found to be less than 50%. Low resistance rates (< 3%) were observed for Salmonella typhi, Salmonella paratyphi A, Shigella flexneri, Serratia marcescens, and Streptococcus pneumoniae. These results could be useful in developing guidelines on the use of ceftriaxone in Indonesia. (Med J Indones 2007; 16:3-6) Keywords: Microbial drug resistance, disc diffusion method, Gram-positive, Gram-negative

Microbiology aspect of wound infection: in-vitro test for efficacy of hydrophobic dressing in microorganism binding Rosana, Yeva; Dewi, Beti E.; Tjampakasari, Conny R.
Medical Journal of Indonesia Vol 18, No 3 (2009): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Aim To do in vitro test to assess the efficacy of hydrophobic dressing CutimedÂ® SorbactÂ® to bind multiresistant bacteriaÂ that caused wound infection, the methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa.Method This was a cross sectional study that was conducted in the Department of Microbiology, Faculty of Medicine,Â University of Indonesia, on January 2009. In-vitro testing of sterile hydrophobic dressing to bind microorganisms wasÂ conducted by counting MRSA and Pseudomonas aeruginosa that were bound to 1 square centimetre of single layersterile hydrophobic dressing (CutimedÂ® SorbactÂ®). Every test was done in triplicate at 0.5, 1, 5, 10, 30 minutes, 1,Â 2, 3, and 4 hours. To compare the hydrophobic dressing capability to bind microorganisms, in vitro testing of sterileÂ conventional dressing to bind microorganisms on 0.5 minutes and 2 hours was done.Result The binding capacity of sterile hydrophobic dressing began at 0.5 minutes and teached a maximum at 2Â hours. Compared with conventional dressing, sterile hydrophobic dressing had more binding capability to MRSA andÂ Pseudomonas aeruginosa.Conclusion Hydrophobic dressing (CutimedÂ® SorbactÂ®) had a higher capability to bind MRSA and PseudomonasÂ aeruginosa compared to conventional dressing. (Med J Indones 2009;18:155-60)Key words: hydrophobicity, MRSA, Pseudomonas aeruginosa

Risk factors for bacterial vaginosis among Indonesian women Ocviyanti, Dwiana; Rosana, Yeva; Olivia, Shanty; Darmawan, Ferry
Medical Journal of Indonesia Vol 19, No 2 (2010): May
Publisher : Faculty of Medicine Universitas Indonesia

Aim To identify risk factors for bacterial vaginosis (BV) among Indonesian women.Methods This is a cross sectional study involving 492 women with age ranged 15-50 years. Vaginal discharge was collected.Â Whiff test and Nugent scoring were utilized to identify BV. Settings are Puskesmas Karawang, Pedes, Cikampek,Tempuran,Â Batalyon 201 Clinic Cijantung, Faculty of Medicine University of Indonesia and Cipto Mangunkusumo Hospital.Results Age of the subjects were 15-25 years old (26.8%), 26 â 40 years old (59.1%), and > 40 years old (14%).Â The mean age was 30.9 years. Marital status of the subjects were not-married (16.9%), married (76.4%), marriedÂ more than once (6.7%). Prevalence of bacterial vaginosis in this study was 30.7% according to Nugentâs score. AgeÂ > 40 years old (OR=3.15 IK 95% = 1.15-1.48) and uncircumcised couple (OR=6.25, IK 95% = 2.54 - 15.38) wereÂ independently and significantly associated with incidence of BV (p<0.05).Conclusions Prevalence of BV in this study was 30.7%. Determinant risk factors of BV were age and uncircumcisedÂ sexual partner. (Med J Indones 2010; 19:130-5)Keywords: bacterial vaginosis, risk factors, vaginal flora

Detection and identification of azithromycin resistance mutations on Treponema pallidum 23S rRNA gene by nested multiplex polymerase chain reaction Gultom, Desy A.; Rosana, Yeva; Efendi, Ida; Indriatmi, Wresti; Yasmon, Andi
Medical Journal of Indonesia Vol 26, No 2 (2017): June
Publisher : Faculty of Medicine Universitas Indonesia

Background: Azithromycin-resistant strains of Treponema pallidum is associated with the mutation of 23S rRNA gene of T. pallidum. Although these strains are now prevalent in many countries, there is no laboratory test kit to detect and identify these mutations. Thus, in this study we developed a nested multiplex polymerase chain reaction (PCR) to detect and identify A2058G and A2059G mutations in 23S rRNA gene.Methods: Three primer sets were designed for nested PCR reactions. To obtain maximum PCR reaction, all parameters were optimized. The specificity of the primer sets was evaluated towards some microorganisms. A sensitivity test was conducted to get detection limit of deoxyribonucleic acid (DNA). Forty-five whole blood specimens were tested by PCR, and positive results were confirmed by the DNA sequencing.Results: The assay could detect at least 4,400 DNA copy number and showed no cross reaction with other microorganisms used in the specificity test. A total 13 of 45 whole blood specimens were PCR positive for T. pallidum, and no single mutations (either A2058G or A2059G) were detected. Two positive specimens were confirmed by the DNA sequencing and showed no mutation.Conclusion: Nested multiplex PCR developed in this study showed a specific and sensitive test for the detection and identification of A2058G and/or A2059G mutations of 23S rRNA T. pallidum gene.

Co-Authors Abas, Ghina Mutiara Andi Yasmon Anggraini Suwarsono, Erike Anis Karuniawati Ariyani Kiranasari Bachti Alisjahbana Bachti Alisyahbana Basri A. Gani Beti E. Dewi Conny R. Tjampakasari Conny Tjampakasari Dick van Soolingen Dwiana Ocviyanti E. Risdiyani Efendi, Ida Effendi, Ida Fera Ibrahim Ferry Darmawan Fithriyah Fithriyah Gultom, Desy A. Gus Permana Subita Herliyana, Lina Ibnu Agus Ariyanto Ida Parwati Ika Ningsih Imaniar, Clara Krisandi, Grady Louisa Ivana Utami Mardiastuti H Wahid Pratiwi Pujilestari Sudarmono Prawoto Prawoto Rahmah Amran Reinout van Crevel Rela Febriani Retno Kadarsih S. Nilawati S. Sunnati Shanty Olivia Siti Aliyah Pradono SJATHA, FITHRIYAH Sri Rezeki SYADZA RHIZKY PUTRI AKHMAD T. M. Sudiro TETSUHIRO MATSUZAWA Tjahjani M. Sudiro TOHRU GONOI Wia Melia Wresti Indriatmi B. Makes

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