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All Journal Global Medical and Health Communication Universa Medicina Jurnal Biomedika dan Kesehatan Jurnal Penelitian dan Karya Ilmiah Lembaga Penelitian Universitas Trisakti JUARA: JURNAL WAHANA ABDIMAS SEJAHTERA Jurnal Abdimas Kesehatan Terpadu Jurnal Indonesia Sosial Sains AMMA : Jurnal Pengabdian Masyarakat Jurnal Kedokteran Meditek
Effendi, Ida
Fakultas Kedokteran, Universitas Trisakti
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Multiplex nested polymerase chain reaction for Treponema pallidum using blood is more sensitive than using serum Effendi, Ida; Rosana, Yeva; Yasmon, Andi; Indriatmi, Wresti
Universa Medicina Vol 37, No 1 (2018)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2018.v37.75-84

Abstract

BackgroundSyphilis is a multistage disease transmitted primarily through sexual intercourse. Nowadays, the polymerase chain reaction (PCR) test for Treponema pallidum has been widely used and is expected to overcome problems in diagnostic tests for syphilis. The Treponema pallidum PCR is influenced by type of specimens, PCR methods and target genes. This study aimed to assess the use of blood and serum in multiplex nested PCR for Treponema pallidum, targeting the 23S rRNA.MethodsA cross-sectional study was conducted from April 2015 - April 2016. Sampling was carried out consecutively among patients with clinical features of secondary syphilis who came to Sexually Transmitted Disease (STD) clinics in Jakarta. All sera were also tested with Rapid Plasma Reagin (RPR) and Treponema pallidum Hemagglutination Assay (TPHA) assay, which was considered as the gold standard for this study. We determined the sensitivity and specificity of the multiplex nested PCR for Treponema pallidum using blood and serum.ResultsPCR test was performed on 122 clinical specimens (61 blood and 61 serum). The positive results of PCR test on blood was 22.95% and serum was 6.56%, while the positive results of serology was 68.85%. The sensitivity of Treponema pallidum multiplex nested PCR on blood was 30.95% compared to serum 9.52% (p=0.006). PCR test on blood is able to detect 3.25 times higher than serum. ConclusionThe use of blood has a higher proportion of positives compared to serum in Treponema pallidum multiplex nested PCR using 23S rRNA target gene.
Microscopic examination using negative staining for rapid diagnosis of syphilis Rosana, Yeva; Effendi, Ida; Indriatmi, Wresti; Yasmon, Andi
Universa Medicina Vol. 41 No. 1 (2022)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2022.v41.64-70

Abstract

BACKGROUNDSyphilis is a global health problem, especially in developing countries including Indonesia. Treponema pallidum, the etiologic agent of syphilis, cannot be cultured in vitro. Syphilis has several clinical manifestations, making laboratory testing a very important aspect of diagnosis. Microscopic examination may support the diagnosis but is rarely used in Indonesia. The aim of this study was to evaluate negative staining using the light microscope to detect T. pallidum in syphilitic lesions. METHODSA cross-sectional study was conducted involving 27 subjects who came to several dermato-venereology clinics in Jakarta. Exudates were collected from genital ulcers, condylomata lata, and dry mucocutaneous rash on palms and soles of syphilis patients. Negative staining using one drop of Indian ink was used to examine for treponemas under the light microscope at 10x100 magnification. RESULTSMicroscopic examination using negative staining showed a few clusters of small and spiral shaped bacteria. Of the 39 specimens from 27 subjects, microscopic examinations were successfully done on 10 specimens. Observations could only be conducted on 5 specimens, 3 (60.0%) of which showed the morphology of spirochetes. This examination is the easiest method for detecting the bacteria. Moreover, the bacteria that were isolated from painless genital ulcers could be observed more clearly than those from erythematous maculopapular lesions. CONCLUSIONTreponema pallidum was successfully detected by microscopic examination in all moist lesions, but was difficult to detect in dry lesions. Negative staining under the light microscope appears to be simple, affordable, and available in most microbiology laboratories in Indonesia.
Edukasi Hidup Sehat Membiasakan Cuci Tangan Sebagai Upaya Pencegahan Penyakit Infeksi Di Era Pandemi Covid Arleen Devita; Yuliana; Ida Effendi; Meiyanti
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 1 No. 2 (2022): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.771 KB) | DOI: 10.25105/jakt.v1i2.15476

Abstract

Rawa Buaya Village is one of the villages in the West Jakarta. This village is a densely populated village with a limited number of health facilities. Based on the 2018 DKI National Basic Health Research (RISKESDAS) data, the proportion of the population aged over 10 years in West Jakarta who behaved properly in washing their hands was only 49.11%. Hand washing is the most important basic technique in the prevention and control of infection transmission because it mechanically removes dirt and dust from the skin surface and reduces the number of pathogenic microorganisms. Because knowledge of clean and healthy living behavior (PHBS) by washing hands with soap is a determining factor in the transmission of infectious diseases such as diarrhea, respiratory infections including Covid-19, education is needed to increase this knowledge. Education by counseling and training activities are carried out online through the Zoom teleconference on April 23, 2022, followed by 45 village residents. Participants' knowledge was assessed by pre-test and post-test using Google Form. From the post-test’s results, there is an increase in the participants’s knowledge by 22.9% with an increase in the percentage of correct answers from the pre and post test ranging from 6.6% - 35.6%, which is also expected to change in habits or behavior.
PENYULUHAN PRAKTIK HIDUP BERSIH DAN SEHAT DI SEKOLAH SELAMA MASA PANDEMI COVID-19 Ida Effendi
JUARA: Jurnal Wahana Abdimas Sejahtera Volume 4, Nomor 1, Januari 2023
Publisher : Jurusan Teknik Lingkungan Fakultas Arsitektur Lanskap dan Teknologi Lingkungan, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/juara.v4i1.13630

Abstract

The prolonged state of the COVID-19 pandemic has a huge impact on all sectors, one of which is the education sector. Since pandemic stated, educational institutions have applied distance learning (PJJ) from pre-school to university level. In its implementation, PJJ is considered effective enough to be carried out in accordance with pandemic conditions. The negative impact of PPJ on student is difficulty in self development and hard to catch up so that limited face-to-face learning (PTM) begins. The purpose of community service activities (PkM) is to increase understanding and awareness of being obedient to health protocols during PTM consistently to prevent the transmission of COVID-19. The activity was given in the form of webinar counseling for students, parents and teachers of SMPIT Al Madinah, Bogor, West Java. Submission of material through presentations and interactively with questions and answers. The result of this PkM activity is an increase in knowledge and behavior in obeying health protocols during PTM. This service activity is needed and provides benefits to the community.
HUBUNGAN BEBAN PERAWATAN DENGAN KUALITAS HIDUP CAREGIVER ORANG DENGAN HIV-AIDS (ODHA) Alya Safira Azhar; Ida Effendi
JURNAL PENELITIAN DAN KARYA ILMIAH LEMBAGA PENELITIAN UNIVERSITAS TRISAKTI Vol. 8 No. 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pdk.v8i2.15226

Abstract

Penyakit infeksi HIV- AIDS telah menjadi masalah darurat global. Data yang diperoleh pada tahun 2021 menunjukkan sebanyak 38.4 juta penduduk di seluruh dunia terinfeksi HIV. Sebagian besar orang dengan HIV-AIDS tidak mampu melakukan aktivitas keseharian dengan baik sehingga membutuhkan orang yang dapat mendampingi serta memberi dukungan psikososial, yaitu caregiver. Ketergantungan orang dengan HIV-AIDS terhadap caregivernya dapat menimbulkan beban pada caregiver. Keadaaan ini bilamana berlangsung jangka panjang dapat berdampak pada kualitas hidup caregiver. Penelitian bertujuan untuk mengetahui hubungan antara beban perawatan ODHA dengan kualitas hidup caregiver/keluarga ODHA. Metode yang digunakan adalah studi potong lintang dilakukan pada caregiver ODHA. Data yang diperoleh dari 56 responden menunjukkan terdapat hubungan yang bermakna antara beban perawatan dengan kualitas hidup pada domain overall quality of life (p=0,025) dan domain psikologis (p=0,035). Beban perawatan ODHA mempengaruhi kualitas hidup caregiver ODHA
Peningkatan Pengetahuan mengenai Bahaya dan Risiko Gangguan Pernapasan pada Pengemudi Ojek Online Arleen Devita; Yuliana; Ida Effendi; Hans Utama Sutanto; Meiyanti
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 2 No. 1 (2023): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v2i1.16794

Abstract

Jakarta is listed as one of the cities in the world with the most polluted air quality, with an annual average PM 2.5 of 49.4 g/m³ in 2019. The sources of air pollution in Jakarta among others are the large number of motor vehicles that are not in accordance with the guidelines of safe vehicles for the environment. Not to mention air pollution from industries and factories fueled by coal or other fossils. Online motorcycle taxi drivers are a profession that is at risk of experiencing the effects of this air pollution. Respiratory disorders that can occur such as acute respiratory infections, chronic obstructive pulmonary disease (COPD), decreased lung function and lung malignancy. To reduce the risk of respiratory problems that can arise in online motorcycle taxi drivers, efforts are being made to prevent and stop exposure to air pollutants through counseling activities which carried out on November 12, 2023. This activity was attended by 42 online motorcycle taxi drivers. Participants' knowledge was assessed by pre-test and post-test using the Google form to assess participants' cognitive changes. From the results of the pre-test and post-test evaluation, it was found that there was an increase in knowledge about the risks and respiratory tract disorders that could arise, namely 25.5%, with an increasing percentage of correct answers of pre-test and post-test ranging from 4.8 % - 40.5%. It is hoped that this increased knowledge will change the behavior of online motorcycle taxi drivers to always pay attention of prevention and stop exposure to air pollutants.
Edukasi Pencegahan Infeksi Saluran Kemih pada Pengemudi Ojek Online Ida Effendi; Arleen Devita; Jihan Samira; Adrianus Kosasih
AMMA : Jurnal Pengabdian Masyarakat Vol. 1 No. 12 (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

 Pekerjaan sebagai pengemudi ojek online memiliki risiko kesehatan terkait jenis pekerjaan. Perilaku Kesehatan yang tidak baik pada pengemudi ojol seperti kurangnya konsumsi air minum dan menahan buang air kecil yang dapat mencetuskan timbulnya infeksi saluran kemih. Informasi perilaku pengemudi ojek online terkait berkemih menunjukkan kebiasaan yang dapat berdampak pada kesehatan.  Perubahan kebiasaan tidak baik dapat terjadi apabila terdapat pengetahuan dan pemahaman terhadap penyakit tersebut. Kegiatan pengabdian kepada masyarakat dilakukan terhadap 57 pengemudi ojek online dalam bentuk edukasi untuk meningkatan pengetahauan dan pemahaman terhadap penyakit infeksi saluran kemih sehingga para pengemudi ojek online dapat mempraktikkan perilaku sehat  dalam keseharian saat bekerja. Penyampaian materi melalui komunikasi langsung dua arah dan pemberian materi edukasi berupa leaflet.  Hasil dari kegiatan PkM ini adalah peningkatan pengetahuan untuk berperilaku sehat terkait kesehatan saluran kemih diantara pengemudi ojek online.
Penyuluhan, Pemeriksaan Mata, Gula Darah Kolesterol Dan Asam Urat Pada Masyarakat Kecamatan Sukmajaya, Depok, Jawa Barat Noviani Prasetyaningsih; Anggraeni Adiwardhani; Riani Witjaksana; Isa Bella; Jihan Samira; Ida Effendi; Monica Dwi Hartanti
AMMA : Jurnal Pengabdian Masyarakat Vol. 2 No. 6 : Juli (2023): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dry eye disease is a disease with a fairly high prevalence worldwide. Old age and women are dominant risk factors for this disease. Other risk factors are systemic diseases such as Diabetes Mellitus and Hypercholesterolemia. External factors can also trigger the onset of this disease, such as ultraviolet rays, low humidity, wind, dust, and pollution, such as cigarette smoke, vehicle exhaust, or pollution from industry. In addition, excessive use of electronic devices such as mobile phones, computers, and laptops can cause the eyes to become tired quickly. Indonesia, which is a country with a lot of sunlight, pollution in big cities, and smoking habits, as well as the high number of DM and cholesterol patients, causes the prevalence of Dry Eye Disease in Indonesia to be quite high. The Community Service Program carried out by the Faculty of Medicine, Trisakti University, with the theme "Education, Eye Examination, Blood Sugar, Cholesterol and Uric Acid in the Sukmajaya District Community, Depok, West Java" was held in March 2023. Residents invited to this activity were those aged 50 years and over, with 50 participants with a female to male ratio of 58% to 42%. Residents who experienced dry eyes were 14%, those with high blood sugar were 20%, those with high cholesterol were 46%, and those with high uric acid were 54%.
Pemeriksaan Molekular Treponema pallidum Ida Effendi
Jurnal Kedokteran Meditek VOL. 24 NO. 68 OKTOBER-DESEMBER 2018
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v24i68.1706

Abstract

Treponema pallidum adalah bakteri penyebab sifilis. Sifilis ditemukan pada abad ke XV di dataran Eropa, menyebar ke seluruh dunia dan menjadi isu global sampai saat ini. Sifilis dikenal dengan sebutan raja singa merupakan salah satu penyakit infeksi menular seksual yang penularannya tidak hanya melalui hubungan seksual. Infeksi lokal yang disebabkan oleh masuknya Treponema pallidum akan berkembang cepat menjadi sistemik dan bahkan dapat mengancam nyawa. Treponema pallidum tidak dapat dikultur secara in vitro. Pemeriksaan penunjang untuk menegakkan diagnosis mengandalkan uji serologi. Saat ini, pemeriksaan standar untuk sifilis menggunakan mikroskop lapangan gelap sulit dilakukan. Pemeriksaan baru untuk mendeteksi Treponema pallidum menggunakan uji molekuler sudah mulai dikembangkan sejak abad XX. Penulisan ini mengambil ulasan dari beberapa jurnal penelitian yang menggunakan PCR sebagai alat diagnostik Treponema pallidum untuk membandingkan metode-metode pemeriksaan molekuler PCR Treponema pallidum. Spesifisitas PCR Treponema pallidum mencapai 100%. Sensitivisitas PCR bervariasi untuk setiap gen target dan jenis spesimen yang digunakan. Metode multiplex PCR banyak digunakan karena dapat mendeteksi Treponema pallidum dan patogen lain secara bersamaan. Pemeriksaan molekuler PCR Treponema pallidum masih perlu dikembangkan lebih lanjut di Indonesia. Kata kunci : Treponema pallidum, sifilis, uji serologi, mikroskop, pemeriksaan molekular
Exploring the Relationship Between Sod1, 2 And 3 Gene Polymorphisms With Post-Covid19 Symptoms Jihan Samira Thabit; Sisca Sisca; Monica Dwi Hartanti; Noviani Prasetyaningsih; Alvionita Kogoya; Arleen Devita; Isa Bela; Ida Effendi
Jurnal Indonesia Sosial Sains Vol. 5 No. 04 (2024): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v5i04.1097

Abstract

The coronavirus disease 19 (COVID-19) has become a challenge for the media world. Even though they have been declared cured, some Covid-19 survivors still have health complaints. Abnormal symptoms, signs, or clinical parameters that persist two weeks or more after the onset of COVID-19 and do not return to their initial healthy state are potentially considered long-term effects of the disease. Although such changes are primarily reported in people with severe and critical illness, lasting effects also occur in individuals with mild infections that do not require hospitalization. This study aims to explore the role of antioxidants on the pathogenesis of Covid19 and its relationship with SOD1, 2 and 3 genomic variations. Getting alternative biomarkers for long covid19 detection. Research Method: The research sample is blood and questionnaires that will be taken from respondents affected by Covid19 a maximum of 6 months before data collection is held. Furthermore, DNA isolation, DNA amplification, cutting with restriction enzymes, and DNA band documentation with gel electrophoresis will be carried out. Symptoms related to systemic are the most common symptoms found in respondents with Long Covid19. A total of 8 respondents had systemic related symptoms, namely weakness, lethargy and sweating, while skin-related complaints were found in three respondents and one respondent had lung-related complaints. The average SOD activity value of respondents was 2.19 U/ml. In this study, more symptoms of long COVID-19 were found associated with systemic and cardiovascular symptoms. The complaints were not differentiated by the sex of the respondents. The output draft will be processed immediately after getting other results.
Co-Authors Adrianus Kosasih Alvionita Kogoya Alya Safira Azhar Andi Yasmon Anggraeni Adiwardhani Azhar, Alya Safira Bela, Isa Bella, Isa Devita, Arleen Florida Kalumpiu, Jane Hans Utama Sutanto Husnun Amalia Isa Bela Isa Bella Jihan Samira Jihan Samira Jihan Samira Thabit Kalumpiu, Joice Khariri Khariri Kogoya, Alvionita Kurniasari Meiyanti Meiyanti Monica Dwi Hartanti Monica Dwi Hartanti Monica Dwi Hartanti, Monica Dwi Noviani Prasetyaningsih Prasetyaningsih, Noviani Riani Witjaksana Robertus, Thomas Samira, Jihan Sisca Sisca Sisca Sisca, Sisca T. Robertus THABIT, JIHAN SAMIRA Widyasari Kumala Wresti Indriatmi B. Makes Yeva Rosana Yuliana Yuliana Yuliana, Yuliana