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All Journal Jurnal Akuakultur Indonesia Torani Journal of Fisheries and Marine Science Jurnal Ecosolum Jurnal Dinamika Pengabdian (JDP) Proceeding Simposium Nasional Kelautan dan Perikanan Aquacultura Indonesiana Journal of Aquaculture and Fish Health Asian Journal of Aquatic Sciences Tomini Journal of Aquatic Science Bambu Laut: Jurnal Pengabdian Masyarakat

Edison Saade

Faculty Of Marine Science And Fisheries, Hasanuddin University, Makassar, Indonesia

Author-ID : 367380

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Social Sciences

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Effect of Predigested Artificial Diet Using Papain Enzyme on the Degree of Protein Hydrolysis and Protease Enzyme Activity of Mud Crab (Scylla olivacea) Larvae at Zoea 2 and 3 Stages Haryati, Haryati; Fujaya, Yushinta; Saade, Edison
Aquacultura Indonesiana Vol 19, No 2 (2018)
Publisher : Indonesian Aquaculture Society (MAI)

The ability of mud crab (Scylla olivacea) larvae to digest artificial diet depends on the availability of digestive enzyme. To enhance the larvaes capability to utilize artificial diet can be conducted by adding exogenous enzyme to the diet.The aim of this research was to determine the dose of papain enzyme and stadia of predigest artificial diet that produced the best degree of protein hydrolysis and protease enzyme activity. Completely randomized designwas used to evaluate the effect of papain enzyme dose on the degree of protein, with four treatment and three replications, namely the doses of 0.0%, 1.5%, 3.0%, and 4.5%. Factorial pattern with the completely randomized design was used to evaluate the effect of papain enzyme dose and the proper predigest artificial feeding stage on the activity of protease enzyme. The first factor was the papain enzyme dose (0.0%, 1.5%, 3.0%, and 4.5%), while second factor was the stadia of larvae when it is fed with predigested artificial diet (zoea 2 and zoea 3 stadia). The research showed that the difference of papain enzyme dose provides real effect on degree of protein hydrolysis either on JP 0 or JP 1 diet brands (P<0.05). Degree of protein hydrolysis of the diet predigested with 0.0% papain enzyme was the lowest and has real difference (P<0.05) with the 1.5%, 3.0% and 4.5% doses. Degree of protein hydrolysis on 3% dose of papain enzyme does not provide real difference with the 4.5%, namely 19.753% and 22.890% respectively in JP 0, 18.707 % and 20.430 % in JP 1, but has real difference (P<0.05) with 1.5% doses. The difference papain enzyme dose and stadia of predigest artificial diet as well as the interaction between both of them had significant effect (p<0.05) on the activity of protease enzyme. On the larvae of zoea 2 stadia, the activity of protease enzyme on the larvae fed with predigest artificial diet starting from zoea 2 stadia with 3.0% and 4.5% papain enzyme dose or those still fed with natural diet does not provide real difference, but is higher and provides real difference than 0% and 1.5% doses. On the larvae of zoea 3 stadia, the activity of protease enzyme to the larvae fed with artificial diet starting from zoea 2 with 0% and 1.5% papain enzyme doses, and those fed with artificial diet starting from zoea 3 stadia with 0% papain enzyme dose does not provide real difference (p>0.05) but is lower and provides real difference than other treatments. Protease enzyme activity on the larvae fed with predigest artificial diet using 4.5% papain enzyme starting at zoea 2 stadia , was not significantly different (P <0.05) compared to larvae fed artificial diet which was predigest with 0.0%, 1.5%, 3.0 and 4.5 papain enzyme started at Zoea 3. Based on the degree of protein hydrolysis, papain enzymes can be used to hydrolyze artificial diet in doses ranging from 3.0% to 4.5%. Based on the activity of protease enzymes, mud crab larvae (Scylla olivacea) predigested artificial diet using 4.5% papain enzyme can be provided to the larvae starting from zoea 2 stadia.

The Effect of Corn Starch Substitution with Sargassum sp. Starch in Diet on Grow-Out of Cultivated Rabbitfish, (Siganus guttatus) in Floating Net Cages Edison Saade; Usman Usman; Haura Ainun Sulaeman; Nursun Marhumatul Jannah
Journal of Aquaculture and Fish Health Vol. 11 No. 1 (2022): JAFH Vol. 11 No. 1 February 2022
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v11i1.22082

Corn starch is a source of carbohydrates for most cultivated fish, includes rabbitfish as one of the herbivores with high economic value. Therefore, rabbitfish requires cheaper carbohydrate sources to have a profitable business in its cultivation. This study was aimed to determine the substitution of corn starch with Sargassum sp. starch in the diet on the growth, relative feed intake, FCR and nutrient retention of rabbitfish. The average weight of rabbitfish used was 51.76±0.12 g/fish. Rabbitfish were cultivated in small net cages (1x1x1.5m) with a stocking density of 20 fish/net cage for 90 days reared. This study used an experimental method with a Completely Randomized Design (CRD). Corn starch (CM) substitution with Sargassum sp. starch (SM) by 0% (A), 21% (B), 42% (C), 63% (D) and 83% (E) used as the treatments with three replicates each. The measured parameters were weight gain (WG), relative growth (RG), relative feed intake (RFI), feed conversion ratio (FCR), protein retention (PR), energy retention (ER), and condition factor (CF). Data were analyzed using the analysis of variance (ANOVA) and Tuckey’s test. The results showed that the lowest FCR in treatment A had no significant effect with treatment B but was significantly different from treatments C, D and E. The WG, RG, RP and CF parameters had the same values at all levels of CM substitution with SM, while RFI and FCR increased with the increasing CM substitution by SM. It also indicated that CM in the rabbitfish diet could be substituted with SM by 83%.

Pengaruh Tingkat Subsitusi Pakan Alami dengan Pakan Buatan terhadap Tingkat Konsumsi Pakan dan Komposisi Asam Amino Ikan Gabus (Channa striata) Haryati; Dody Dh. Trijuno; Edison Saade
Prosiding Simposium Nasional Kelautan dan Perikanan Vol. 7 (2020): PROSIDING SIMPOSIUM NASIONAL VII KELAUTAN DAN PERIKANAN UNHAS
Publisher : Fakultas Ilmu Kelautan dan Perikanan (FIKP), Universitas Hasanuddin

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Ikan gabus (Channa striata) saat ini tidak hanya sebagai sumber protein hewani, tetapi komoditas tersebut juga dimanfaatkan untuk industri farmasi yaitu sebagi sumber albumin. Selama ini pakan yang digunakan untuk pemeliharaan ikan gabus adalah pakan alami, namun pakan alami mempunyai beberapa kelemahan, oleh karena itu perannya perlu digantikan oleh pakan buatan. Tujuan penelitian ini adalah untuk mengetahui pengaruh tingkat subsitusi pakan alami dengan pakan buatan terhadap tingkat konsumsi pakan, komposisi asam amino pakan dan komposisi asam amino pada ikan gabus. Perlakuan dalam penelitian ini yaitu: A) 100% pakan pakan alami, B) 75% pakan alami dan 25% pakan buatan, C) 50% pakan alami dan 50% pakan buatan, D) 25% pakan alami dan 75% pakan buatan dan E) 100% pakan buatan. Parameter yang di evaluasi yaitu (1) tingkat konsumsi pakan, (2) komposisi asam amino pakan dan (3) komposisi asam amino ikan gabus. Hasil penelitian menunjukkan bahwa perbedaan tingkat substitusi pakan alami dengan pakan buatan tidak berpengaruh nyata (p>0.05) terhadap tingkat konsumsi pakan. Kandungan asam amino pada pakan alami lebih tinggi dibandingkan pada pakan buatan. Kandungan total asam amino ikan gabus paling tinggi pada perlakuan A (75.08%), kemudian diikuti perlakuan B (69,56%), sedangkan pada perlakuan C, D dan E berturut-turut 49,29%, 45,65% dan 41,96%.Kata kunci: asam amino, ikan gabus (Channa striata), pakan alami, pakan buatan, tingkat konsumsi pakan, substitusi

PELATIHAN DAN PENDAMPINGAN BUDIDAYA IKAN NILA DI KOLAM TERPAL RESIRKULASI DAN TEKNOLOGI PEMBUATAN PAKANNYA DI PONDOK PESANTREN MODERN ISLAM SHOHWATUL IS’AD KABUPATEN PANGKEP SULAWESI SELATAN Saade, Edison; Andriani, Irma; Hidayani, Andi Aliah
Jurnal Dinamika Pengabdian Vol. 9 No. 2 (2024): JURNAL DINAMIKA PENGABDIAN VOL. 9 NO. 2 MEI 2024
Publisher : Departemen Budidaya Pertanian Fakultas Pertanian UNHAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jdp.v9i2.32174

Ikan nila, Oreochromis niloticus salah satu ikan air tawar yang sangat digemari masyarakat saat ini. Selain kandungan nutrisinya yang cukup tinggi juga rasa dagingnya sangat lezat. Hal lain yang menjadikan ikan nila ini memuncak trendnya adalah mudah penangananannya dalam budidaya dan teknologi pakannya sudah dikuasai. Namun demikian, teknologi budidaya baik pada usaha pembesaran dan produksi benihnya serta teknologi pembuatan pakannya belum memasyarakat oleh para praktisi. Pondok Pesantren Modern Islam (PPMI) Shohwatul Is’ad (Shohid) berlokasi di Kabupaten Pangkajene Kepulauan berperan sebagai mitra pada kegiatan ini dengan harapan PPMI Shohid sebagai institusi swasta mampu meningkatkan finansialnya secara mandiri melalui budidaya ikan nila di kolam terpal dengan Recirculating Aquaculture System (RAS). Permasalahannya adalah pengetahuan dan keterampilan SDM meliputi guru, tenaga kependidikan, dan santri tentang hal-hal tersebut di atas masih terbatas. Solusinya adalah upaya peningkatan pengetahuan dan keterampilan SDM melalui pelatihan, demonstrasi dan pendampingan. Luaran kegiatan pengabdian masyarakat ini adalah publikasi di jurnal nasional, publikasi media cetak, video dan teknologi tepat guna berupa pakan buatan yang telah dikemas. Tahapan kegiatan yang dilakukan untuk mewujudkannya adalah audiensi dengan Yayasan PPMI Shohid, survey lokasi, persiapan bahan dan alat, pelatihan meliputi budidaya ikan di kolam terpal resirkulasi, pembuatan pakan dan teknik produksi benih ikan nila jantan, pemasangan kolam terpal dengan RAS, penebaran ikan, monitoring, pemeliharaan ikan nila, dan panen. Kegiatan pengabdian dinyatakan sukses dengan indikator PPMI Shohid mampu memeliharan dan memproduksi ikan nila di kolam terpal resirkulasi dengan upaya mandiri. Kata kunci: Ikan nila, kolam terpal, pakan buatan, PPMI Shohid. ABSTRACT Tilapia, Oreochromis niloticus, is one of the freshwater fish that is very popular with people today. Apart from its high nutritional content, the meat also tastes very delicious. Another thing that makes this tilapia fish peak in popularity is that it is easy to handle in cultivation and the feed technology has been mastered. However, cultivation technology, both in growing and producing larvae, as well as technology for making feed, has not yet become popular among practitioners. Islamic Modern Islamic Boarding School (PPMI) of Shohwatul Is'ad (Shohid) as a partner is located in Pangkajene Islands Regency is expected to be able to manage and meet its financial needs independently. The problem is that the knowledge and skills of human resources including teachers, education staff and students regarding fish cultivation in tarpaulin ponds are still limited. The solution is an effort to increase the knowledge and skills of human resources through training, demonstrations and mentoring. The stages of activities carried out to make this happen are an audience with the PPMI Shohid Foundation, site survey, preparation of materials and tools, training including cultivating fish in recirculating tarpaulin ponds, making feed and production techniques for male tilapia fish seeds, installing tarpaulin ponds with RAS, stocking fish, monitoring, raising tilapia, and harvesting. The service activity was declared successful with the PPMI Shohid indicator being able to raise and produce tilapia in recirculating tarpaulin ponds with independent efforts. Keywords: Tilapia fish, tarpaulin pond, artificial feed, and PPMI Shohid.

Produksi Padi (Oriza sativa L) dan Ikan Mas (Cyprinus carpio) pada Berbagai Pengelolaan Air, Jenis Varietas dan Dosis Pakan pada Sistem Minapadi Makmur, Makmur; Yassi, Amir; Saade, Edison
Prosiding Simposium Nasional Kelautan dan Perikanan Vol. 10 (2023): PROSIDING SIMPOSIUM NASIONAL X KELAUTAN DAN PERIKANAN UNHAS
Publisher : Fakultas Ilmu Kelautan dan Perikanan (FIKP), Universitas Hasanuddin

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Penelitian bertujuan mengetahui metode pengelolaan air, jenis varietas dan dosis pakan ikan serta interaksi ketiganya yang memberikan produksi tinggi tanaman padi dan ikan mas pada sistem minapadi. Penelitian ini dilaksanakan di Desa Salumokanan, Kabupaten Mamasa, Sulawesi Barat pada bulan Januari – Juni 2022. Rancangan yang digunakan adalah Rancangan Petak-Petak Terpisah dengan metode pengelolaan air sebagai petak utama, jenis varietas sebagai anak petak dan dosis pakan ikan mas sebagai anak-anak petak. Metode pengelolaan air (P) sebagai petak utama yang terdiri atas 2 taraf yaitu sistem pengelolaan basah kering / AWD (P1), sistem pengairan tergenang (P2). Anak petak adalah varietas padi yang terdiri atas 2 taraf yaitu varietas lokal mamasa (V1) dan Inpari 30 (V2). Anak-anak petak adalah dosis pakan ikan (D) yang terdiri atas 3 taraf yaitu 1 % bobot ikan (D1), 3 % bobot ikan (D2) dan 5 % bobot ikan(D3). Hasil penelitian pengelolaan air tergenang memberikan hasil tertinggi untuk jumlah anakan produktif (12,78 batang), berat kering tanaman (40,81 g), pertumbuhan bobot mutlak (73,06 g), laju pertumbuhan spesifik (1,52 %) dan tingkat kelangsungan hidup (94,44 %). Pengelolaan air basah kering hasil tertinggi pada produksi gabah per petak (8,77 Kg). Varietas inpari 30 hasil terbaik pada berat kering tanaman (41,25 g), produksi gabah per petak (8,69 g), pertumbuhan bobot mutlak (72,61 g) dan laju pertumbuhan spesifik (1,51 g). Dosis pakan ikan 5 % hasil tertinggi pada jumlah anakan produktif (12,42 g), pertumbuhan bobot mutlak (75,76 g) dan laju pertumbuhan spesifik (1,54 %). Intraksi pengelolaan air tergenang dan dosis pakan ikan 5 % hasil terbaik pada pertumbuhan bobot mutlak (86,00 g) dan laju pertumbuhan spesifik (1,63 %). Interaksi varietas inpari 30 dan dosis pakan ikan 5 % memberikan hasil terbaik pada jumlah anakan produktif (13,33 batang), pertumbuhan bobot mutlak (84,33 g) dan laju pertumbuhan spesifik (1,61 %). Interaksi pengelolaan air tergenang, varietas inpari 30 dan dosis pakan ikan 5 % hasil terbaik pertumbuhan bobot mutlak (103,33 g) dan laju pertumbuhan spesifik (1,78 %).

EFFECT OF FEED RAW MATERIALS TYPES ON DISSOLVED PROTEIN CONTENT, PROTEIN HYDROLYSIS LEVEL AND FEED NUTRITION CONTENT OF ORANGE MANGROVE CRAB (Scylla olivacea) LARVAE FEED Haryati, Haryati; Fujaya, Yushinta; Saade, Edison
Asian Journal of Aquatic Sciences Vol. 1 No. 1 (2018): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.039 KB)

Feed is one of the factors that affect the success of hatchery culture of mud crab (Scylla olivacea). The use of artificial feed in the form of microdiet can ensure availability, lower production costs and higher flexibility than natural feed. During this time the artificial feed used is a commercial feed, which is relatively expensive, therefore the need for research to produce artificial feed of good quality, with a cheap price.. The objective of this research is to determine the combination of raw material of artificial feed protein which produce dissolved protein content, protein hydrolysis degree and best protein content of feed. The experimental design used was completely randomized with eight treatments and three replications. The treatments were the use of various combinations of feed protein ingredients : A) 100% Artemia flour, B) 100% fish meal, C) 100% squid meal, D) 50% Artemia and 50% fish meals, E) 50% Artemia and 50% squid meals, F) 50% fish and 50% squid meals, G) 35% fish, 35% squid and 30% Artemia meals, H) commercial feed. The parameters used were dissolved protein content, protein hydrolysis degree and protein feed content. The results showed that the difference of feed protein raw materials had significant effect (p <0,05) on soluble protein content and feed protein hydrolysis degree. The highest content of soluble protein in in the diet with a protein raw material was squid meal, but not significantly different (P> 0,05) than Artemia meal and combination between 50% Artemia and 50% squid meals, but significantly different (P < 0.05) than other treatments. The highest degree of protein hydrolysis in the diet with a protein raw materials was Artemia meal, but not significantly different (P> 0.05) than in commercial feed as well as combination between Artemia and squid meal (P> 0.05), but significantly different (P <0, 05) compared to other treatments. Feed protein content according to the needs of mangrove crab larvae. Fat content with a protein raw materials was 100% artemia meal was higher than the need. Carbohydrate content with a protein raw materials were 100% artemia meal, a combination of 50% artemia meal and 50% squid meal and commercial feed according to the needs of mud crab larvae

Quality of tiger shrimp artificial feed using various dosages of seaweed (Gracilaria gigas) meal as binder Saade, Edison; Aslamyah, Siti; Salam, Nur Insana
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The quality of an artificial feed particularly the stability of the feed in the water is highly determined by binding agents. The objective of this study was to determine the optimum dosage of seaweed, Gracilaria gigas meal as binder. Completely randomized design was used in this study, with three dosages of sea weed meal as treatment feeds, i.e. 3, 6, and 9%, with three replications. The control feed was a commercial feed. The physical parameters measured were water stability, hardness level, homogeneity level, sinking rate, attractiveness, and palatability of the feed, and the chemical parameters were protein and lipid dispersion. Based on parameters of hardness level, homogeneity level, protein and lipid dispersion, feed used G. gigas meal of 9% was better than the commercial feed; parameters of sinking rate, attractiveness, and palatability with G. gigas of 9% was equal to the commercial feed; and based on parameters of water stability, the commercial feed was better than treatment feeds. However, when the three treatment feeds were compared, the 9% dosage of G. gigas meal was better than the other two dosages. Based on the results, the feed using seaweed, G. gigas meal of 9% was the best feed. Key words: Artificial feed, binder, feed quality, G. gigas meal ABSTRAK Bahan perekat sangat menentukan kualitas pakan buatan, terutama stabilitas dalam air. Tujuan penelitian ini adalah untuk menentukan dosis tepung rumput laut, Gracilaria gigas yang terbaik sebagai bahan perekat. Penelitian didesain menggunakan rancangan acak lengkap, dengan tiga dosis tepung G.gigas, yaitu 3, 6, dan 9% yang diulang tiga kali. Pakan komersial digunakan sebagai pakan kontrol. Parameter fisik yang diukur adalah water stability (kecepatan pecah dan dispersi padatan), tingkat kekerasan, tingkat homogenitas, kecepatan tenggelam, daya pikat dan daya lezat pelet, sedangkan parameter kimiawi adalah dispersi protein dan lemak. Berdasarkan parameter tingkat kekerasan, tingkat homogenitas, dispersi potein dan dispersi lemak pakan yang menggunakan binder tepung G. gigas 9% lebih baik dibanding dengan pakan komersial; parameter kecepatan tenggelam, daya pikat dan daya lezat pakan yang mengandung binder G. gigas 9% sama dengan pakan komersial; dan berdasarkan water stability, pakan komersial lebih baik dibanding dengan pakan yang menggunakan binder tepung rumput laut G. gigas. Namun bila ketiga pakan perlakuan dibandingkan, pakan dengan binder G. gigas 9% masih lebih baik dibanding dengan kedua pakan perlakuan lainnya. Sesuai hasil penelitian ini, pakan yang menggunakan binder tepung rumput laut, G. gigas dengan dosis 9% adalah pakan terbaik. Kata kunci: kualitas pakan, binder, tepung G. gigas, pakan buatan

Growth response of koi fish fed on the diet containing Euchema cottoni Saade, Edison; Trijuno, Dody D; Haryati, ,; Zainuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Seaweed meal has been used as ingredient, binding and thickening agent in the diet. The objective of this study was to examine the biological response of Cyprinus carpio haematopterus consumed several types of gel feed using Euchema cottoni as thickening agent. Four types of diets containing different level of moisture were used i.e. 60−90% (treatment A), 30−60% (treatment B), 10−30% (treatment C), and <10% (treatment D). C. carpio haematopterus with average body weight of 13.58±1.31 g were used in this study. Fish were cultured for six weeks and stocked at the density of 15 fish/aquarium. The completely randomized design with four treatments and two replications were used in this study. Survival, growth rate, and feed efficiency were used as evaluating parameters. According to ANOVA results, the effect of treatments on all tested parameters were similar or not significant (P>0.05). This findings have indicated that the different types of gel diets (wet, semi-wet, semi-dry, and dry) had similar effects on survival, growth, and feed efficiency in C. carpio haematopterus. Keywords: gel feed, Cyprinus carpio haematopterus, diet type, growth, feed efficiency ABSTRAK Tepung rumput laut telah dapat digunakan sebagai bahan baku binder (bahan perekat), dan thickening agent (bahan pengental). Tujuan penelitian ini adalah untuk menguji respons biologis ikan koi, Cyprinus carpio haematopterus yang mengkonsumsi beberapa tipe pakan gel yang menggunakan tepung rumput laut, Euchema cottoni sebagai bahan pengental pakan. Bahan baku utama pakan uji adalah tepung ikan, tepung kepala udang, dedak halus, minyak ikan, vitamin dan mineral mix, carboxymethil cellulose (CMC), serta tepung E. cottoni. Empat macam pakan digunakan dalam penelitian ini, yaitu pakan gel basah dengan kandungan air 60−90% (perlakuan A), pakan gel semibasah dengan kandungan air 30−60% (perlakuan B), pakan gel semikering dengan kandungan air 10−30% (perlakuan C), dan pakan gel kering dengan kandungan air <10% (perlakuan D). Ikan uji yang digunakan adalah ikan koi dengan bobot rata-rata 13,58±1,31 g dengan kepadatan 15 ekor/unit pelakuan. Rancangan acak lengkap dengan empat perlakuan dan dua ulangan digunakan dalam penelitian ini. Parameter yang diukur adalah kelangsungan hidup, pertumbuhan dan efisiensi pakan. Berdasarkan hasil ANOVA, pengaruh perlakuan terhadap ke semua parameter uji adalah sama (P>0,05). Hal ini berarti bahwa tipe pakan gel yang berbeda (basah, semibasah, semikering, dan kering) memiliki efek yang sama terhadap kelangsungan hidup, pertumbuhan dan efisiensi pakan pada ikan koi. Kata kunci: pakan gel, Cyprinus carpio haematopterus, tipe pakan, pertumbuhan, efisiensi pakan

Growth performance and efficiency of protein utilization in giant tiger prawn, Penaeus monodon reared in tarpaulin pond with and without faecal chamber Saade, Edison; Fachruddin, Liestiaty; Anshary, Hilal; Lante, Samuel; Haryati, Haryati; Said, Rosmala D.; Wanda, Amalia; Arlini, Fitriwi
Tomini Journal of Aquatic Science VOLUME 2 ISSUE 2, NOVEMBER 2021
Publisher : Gorontalo State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/tjas.v2i2.13347

The accumulation of organic matter at the bottom of the pond greatly affects nutrient metabolism, feed efficiency, growth and productivity in giant tiger prawn cultivation. One of the causes is improper pool bottom construction The purpose of this study was to compare the growth performance and efficiency of protein utilization in giant tiger prawns consuming artificial feed supplemented with multi-enzymes and reared in tarpaulin ponds constructed between not and using the faecal chamber (FAMBER) or the space of metabolic waste and unconsumptioned feed (SOMECOF) at the bottom of the pond with a recirculating aquaculture system (RAS). A tarpaulin pond with a diameter of 3 m and a height of 100 cm filled with 80 cm of seawater. The tarpaulin pond is equipped with a transparent plastic roof, seawater and aeration installation, and a filter in the water pump tank. The average body weight of the experimental prawn used were 8-9 g. The feed given is pellet type artificial feed supplemented with multi-enzymes at a dose of 30 cc/kg of feed. The frequency of feeding were four times a day, namely at 07:00; 12:00; 17:00 and 22:00 with a dose of 5%. The parameters measured were growth performance including growth, condition factors and feed efficiency, while the efficiency of protein utilization included protein content, protein efficiency ratio, amino acid profile, index and score of essential amino acids of feed and experimental prawn. Data analysis was done descriptively. The results showed that all parameters of growth performance and efficiency of protein utilization in tiger prawns reared in tarpaulin ponds using FAMBER were better than those without FAMBER. Based on the results of the study, it was concluded that keeping giant tiger prawns in a tarpaulin pond using a FAMBER is better than not using a FAMBER.

Effectiveness of Addition Papain Enzyme in Artificial Diet on the Metamorphosis Rate and Glycogen Content of Mangrove Crab Larvae (Scylla olivacea) Haryati, Haryati; Fujaya, Yushinta; Saade, Edison; Fajrianti, Dwi
Torani Journal of Fisheries and Marine Science VOLUME 1 NOMOR 2, JUNI 2018
Publisher : Hasanuddin University

In the rearing of fish larvae, artificial feed can be given when digestive enzymes have been produced. To improve the ability of fish larvae to utilize artificial feeds can be done with the addition of exogenous enzyme into the artificial feeds. The aim of this study was to determine the dose of papain enzyme and the correct predigest artificial feeding stadium on metamorphosis rate and glycogen content of mangrove larvae (Scylla olivacea). The experimental design used was a factorial pattern with a complete randomized baseline design, the first factor being the dose of papain enzyme (0%, 1.5%, 3.0% and 4.5%), while the second factor was the predigest artificial feeding stage (zoea 2 and 3). The results showed that the difference of dose of papain enzyme and predigest artificial feeding stages had significant effect (p <0.05) on the metamorphosis rate of 7, 9, 10, 12, 13, 16 and 17 day larvae, but the interaction between them was not significant (p>0,05). The rate of metamorphosis at doses of enzyme papain was 0% and 1.5% longer and significantly different (p <0.05) compared to 3% and 4.5% doses. The rate of metamorphosis at doses of 3.0% and 4.5% was not significantly different (p> 0.05). The content of glycogen larvae in dose of enzyme papain 4,5% not different than dose 1,5% and 3,0% but higher and significantly different than dose 0%. Glycogen content at dose 0%, 1,5% and 3,0%. was not significantly different (p> 0.05). Based on the results of these studies to hydrolyze feed proteins can be used papain enzyme with a dose of 4.5%.Keywords: papain enzyme, metamorphosis rate, mangrove crab larvae (Scylla olivacea), glycogen content

Co-Authors , Haryati, , Abbas, Muh. AMIR YASSI Andi Aliah Hidayani Arlini, Fitriwi Azis, Hasni Y. Badraeni, Badraeni Burhanuddin Rasyid, Burhanuddin Dody D Trijuno, Dody D Dody Dh. Trijuno Fachruddin, Liestiaty Fajrianti, Dwi Gunarto Latama, Gunarto Haryati Haryati Haryati Haura Ainun Sulaeman Hilal Anshary Irfan Ambas Irma Andriani M. Iqbal Djawad, M. Iqbal Makmur Makmur Marlina Achmad Marselianti Muh. Yusri Karim Nur Insana Salam Nurkamilah, Besse Tenri Nursun Marhumatul Jannah Rustam Rustam Said, Rosmala D. Samuel Lante, Samuel Siti Aslamyah Trijuno, Dody Dh. Usman Usman Wanda, Amalia Yushinta Fujaya Zainuddin, . Zainuddin, Elmi N. Zainuddin, Zainuddin

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