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All Journal Klinikal Sains (Jurnal Analis Kesehatan) JURNAL KESEHATAN PERINTIS INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Medika Cendikia (e-Journal) Jurnal Ilmiah Farmako Bahari Science Midwifery Jurnal Sains dan Teknologi Laboratorium Medik Jurnal Kesehatan Jurnal Abdi Masyarakat Kita Jurnal Kesehatan Amanah Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Mukhtabar: Journal of Medical Laboratory Technology Jurnal Riset Ilmu Kesehatan Umum dan Farmasi Jurnal Pengabdian Masyarakat dan Riset Pendidikan Journal of Innovative and Creativity Compromise Journal : Community Proffesional Jurnal Ilmiah Ilmu Kesehatan
Mamay
Department Of Medical Laboratory Technology, STIKes Karsa Husada, Garut, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : indonesian journal of medical laboratory science and technology

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UTILIZATION OF 1% OF METHYLENE BLUE IN STAINING HISTOPATHOLOGICAL PREPARATIONS AT ANATOMIC PATHOLOGY LABORATORY Tri Rahmawati; Yadi Apriyadi; Mamay
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1563

Abstract

Tissue staining using hematoxylin-eosin (HE) is a standard method of histopathological staining. The tissue staining is hampered when there is no hematoxylin reagent in laboratory. Therefore, other reagents are needed that can replace the use of hematoxylin. Methylene blue is a basic dyes that interact with cell nuclei which has a negative ionic charge of the tissue. It can be used as an alternative nuclei staining. This study aims to evaluate the use of 1% of methylene blue in cell nuclei staining in histopathological preparations. The research sample were 15 pathology preparations which were randomly selected including breast cancer, cervical cancer and ovarian cancer in the bank of sampel at anatomical pathology laboratory of RSUD Dr. Slamet Garut, Indonesia. The experiment showed that the methylene blue dyes yielded “worth” result (40%) and “poorly” result (60%). Further research can be carried out by modifying the pH of 1% of methylene blue reagent so that it can maximize the staining preparations results as good as those using hematoxylin.
Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining Mamay Mamay; Ernawati Ernawati; Astari Nurisani
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.3961

Abstract

Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology.
Co-Authors Aceng Ali Awaludin Acep Gunawan Aldi Renaldi Septian Ana Hidayati Mukaromah Arsylia Intan Zori Ichsani Astari Nurisani Dendi Leona Diah Wardani Diah Wardani Eli Laelawati Elsa Zalfa Agustin Fathul Hakim Gina Nafsa Mutmaina Gina Nafsa Mutmaina Gina Nafsa Mutmainah Gina Nafsa Mutmainna Gina Nafsa Mutmainna Iin Patimah Iwan Wahyudi Lia Mar’atiningsih M. Hadi Sulhan Meti Rizki Utari Muhammad Hadi Sulhan Muuhammad Hadi Sulhan N Ai Erlinawati N.Ai Erlinawati Nita Wahyuni Nurani Ai Erlinawati Nurul Nurul Purwanto AP Raihan Nur Aulia Ranti Nurwantika Riki Ananda Rina Herlina Sugiah Sugiah Sugiah Tantri Puspita Tantri Puspita Titi Purwitasari Tri Rahmawati Wisnu Arya Pratama Yadi Apriyadi Zahara Farhan