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Pengaruh Konsentrasi Hidrolisat Kulit Pisang Raja Terhadap Produksi Biohidrogen Menggunakan Escherichia Coli Melalui Dark Fermentation Atqiyaa, Millatul; Fitriani, Ana; Keryanti; Ramadhani, Laily Isna
Prosiding Industrial Research Workshop and National Seminar Vol. 16 No. 1 (2025): Vol. 16 No. 1 (2025): Prosiding 16th Industrial Research Workshop and National
Publisher : Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/irwns.v16i1.6680

Abstract

Biohidrogen merupakan sumber energi terbarukan yang dihasilkan melalui proses biologis dan berpotensi menjadi alternatif pengganti bahan bakar fosil. Penelitian ini bertujuan untuk memproduksi biohidrogen melalui proses dark fermentation dengan menggunakan hidrolisat kulit pisang raja sebagai substrat dan bakteri Escherichia coli sebagai inokulum. Kulit pisang raja dihidrolisis secara enzimatis menggunakan enzim α-amilase dan glukoamilase, menghasilkan gula pereduksi dalam hidrolisat sebesar 7,60% (b/v). Variasi konsentrasi hidrolisat terhadap volume media fermentasi yang digunakan dalam proses fermentasi adalah 20%, 30%, 40%, dan 50% (v/v) pada suhu 37 °C selama 7 hari. Hasil penelitian menunjukkan bahwa pertumbuhan mikroorganisme E. coli meningkat seiring dengan peningkatan konsentrasi hidrolisat, dengan berat sel kering tertinggi pada konsentrasi 50% yaitu sebesar 2,59 g/L. Volume gas tertinggi dihasilkan pada konsentrasi hidrolisat 30%, yaitu sebesar 12,56 mL. Namun, analisis menggunakan GC-TCD menunjukkan bahwa gas biohidrogen tidak terdeteksi pada hasil fermentasi semua variasi konsentrasi. Hal ini mengindikasikan perlunya optimasi lebih lanjut terhadap kondisi fermentasi, termasuk pengendalian pH dan pemilihan mikroorganisme yang lebih sesuai, untuk meningkatkan produksi biohidrogen.
Strategi Pengelolaan Bisnis Budidaya Maggot Black Soldier Fly (BSF) dalam Akselerasi Program Kampus Zero Waste: Studi Kasus Politeknik Negeri Bandung Dewi Amalia; Aceng Gima Sugiama; Yackob Astor; Harita Nurwahyu Chamidy; Keryanti; Ira Puspitasari
Journal of Innovative and Creativity Vol. 6 No. 1 (2026)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v6i1.7478

Abstract

Institusi pendidikan tinggi menghadapi tantangan signifikan dalam mengelola limbah organik. Studi ini mengevaluasi proyek percontohan budidaya larva lalat Black Soldier Fly (BSF) sebagai solusi ekonomi sirkular di Politeknik Negeri Bandung (POLBAN). Menggunakan pendekatan metode campuran konvergen paralel, penelitian ini menggabungkan data operasional kuantitatif dengan analisis pemangku kepentingan kualitatif. Hasil menunjukkan bahwa sistem mencapai indeks pengurangan limbah sebesar 90,07%, dengan pemrosesan limbah organik hingga 1,57 ton per bulan secara konsisten. Efisiensi produksi meningkat secara signifikan, dengan Rasio Konversi Pakan (FCR) turun dari 19,9:1 menjadi 2,7:1 dalam tiga bulan. Secara finansial, proyek ini mencapai kemandirian dalam pembibitan, dan menghilangkan biaya benih. Penelitian ini menyediakan peta jalan strategis untuk mengintegrasikan teknologi biokonversi ke dalam kebijakan pengelolaan limbah institusional.
Optimization of Agitation Speed and Aeration Rate for Fungal Protein Production from Tofu Whey Using Aspergillus oryzae in a Stirred Tank Bioreactor Keryanti, Keryanti; Manfaati, Rintis; Fauzan, Rizky; Ramadhani, Fauziah; Krista, Gustin Mustika; Ferawati, Yohana Fransiska; Santoso, Budi
Jurnal Teknik Kimia dan Lingkungan Vol. 10 No. 1 (2026): April 2026
Publisher : Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33795/jtkl.v10i1.7813

Abstract

Fungal protein, derived from microbial biomass, offers a sustainable protein source and can be produced through fermentation. However, the utilization of tofu whey, an abundant agro-industrial by-product in Indonesia, as a substrate for fungal protein remains underexplored. This study optimizing both agitation speed and aeration rate for Aspergillus oryzae fermentation in a stirred-tank bioreactor. Fermentation was conducted in a 5 L stirred-tank bioreactor with a 3 L working volume for 48 hours at an initial pH of 5 and a temperature of 35℃. Agitation speeds of 150, 200, 250, and 300 rpm were tested at a constant aeration rate of 1.0 vvm to determine the optimum mixing condition. The agitation speed that yielded the highest dry cell weight was then used as the basis for further aeration experiments (0, 0.5, 1.0, and 1.5 vvm). The optimum conditions were obtained at 150 rpm and 1.0 vvm, resulting in a dry cell weight of 7.1 g/L and a protein content of 6.83% (w/w). These findings demonstrate the potential of valorizing tofu whey into fungal protein while highlighting the need for further multi-parameter optimization to enhance protein levels toward single-cell protein standards.
Sintesis Carbon Nanodots dari Molase Menggunakan Metode Pemanasan Terbantukan Gelombang Mikro Hulupi, Mentik; Nabilah, Nida; Nabilah, Tiana Hasna; Keryanti, Keryanti; Abdilah, Fauzi
Equilibrium Journal of Chemical Engineering Vol 6, No 1 (2022): Volume 6, No 1 July 2022
Publisher : Program studi Teknik Kimia UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/equilibrium.v6i1.61212

Abstract

ABSTRAK. Carbon nanodots (CNDs) merupakan nanomaterial yang berukuran dibawah 10 nm. CNDs memiliki keunggulan diantaranya yaitu fotostabilitas tinggi, biokompatibilitas baik, toksisitas yang rendah, mudah dibuat, dan ramah lingkungan. Sumber karbon yang digunakan berasal dari molase karena murah dan mudah didapat serta merupakan hasil samping dari pengolahan tebu. Pada penelitian ini digunakan metode pemanasan terbantukan gelombang mikro atau Microwave Assisted Extraction (MAE) yang memberikan hasil suspensi yang homogen, pemanasan efisien, dan waktu yang singkat. Waktu iradiasi mempengaruhi energi gap CNDs dari molase yang dihasilkan. Waktu yang optimum untuk sintesis CNDs dari molase menggunakan metode MAE yaitu selama 3 menit. Efek fluoresensi telah diamati untuk suspensi CNDs yang mengubah warna kuning menjadi warna biru dibawah sinar UV 366 nm. Karakterisasi menggunakan spektrofotometri UV-Vis menunjukan serapan maksimum CNDs dari molase pada panjang gelombang 255 nm dengan energi gap sebesar 4,55 eV.Kata kunci: Carbon Nanodots, Microwave, MolaseABSTRACT. Carbon nanodots (CNDs) are nanomaterials with a size below 10 nm. CNDs have advantages such as high photostability, good biocompatibility, low toxicity, easy to manufacture, and environmentally friendly. The carbon source used comes from molasses because it is cheap and easy to obtain and is a by-product of sugarcane processing. In this study, the Microwave Assisted Extraction (MAE) method was used which gave homogeneous suspension results, efficient heating, and short time. The irradiation time affects the energy gap of the CNDs of the molasses produced. The optimum time for the synthesis of CNDs from molasses using the MAE method is 3 minutes. Fluorescence effects have been observed for suspensions of CNDs which turn yellow to blue under 366 nm UV light. Characterization using UV-Vis spectrophotometry showed the maximum absorption of CNDs from molasses at a wavelength of 255 nm with an energy gap of 4.55 eV.Keywords: Carbon Nanodots, Microwave, Molasses 
Validation of Methylene Blue Analysis Method in Wastewater Samples by UV-Vis Spectrophotometry Hulupi, Mentik; Keryanti, Keryanti; Rahmawati, Karina Aulia; Dewi, Widya Tresna; Abdilah, Fauzi
Equilibrium Journal of Chemical Engineering Vol 7, No 2 (2023): Volume 7, No 2 December 2023
Publisher : Program studi Teknik Kimia UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/equilibrium.v7i2.75807

Abstract

Abstract. Methylene blue (MB) is an extensively utilized cationic dye in the textile industry. MB is, however, a highly noxious substance that can have detrimental effects on both the environment and human health. MB can pollute waterways and cause the mortality of aquatic organisms in the environment. Due to the hazards posed by MB, it is crucial to have an effective method for analyzing its concentration in wastewater. This will ensure that MB-contaminated water is appropriately treated and disposed of, thereby protecting the environment and human health. One of the analyses utilized the Evolution 360 UV-Vis Spectrophotometer to determine methylene blue concentrations. The Evolution 360 UV-Vis Spectrophotometer method was validated by measuring linearity tests, limit detection and quantization, precision, and accuracy. The R-value for linearity measurements is greater than 0.99, indicating that the method is proportionally validated. As required, the precision measurement yields acceptable results, with a %RSD value of less than 2%, and the accuracy measurement yields a recovery of 100% (between 80 and 110%). So that the method for measuring the concentration of methylene blue in water using the UV-Vis Evolution 360 Spectrophotometer satisfies the requirements for linearity, precision, and accuracy.Keywords:Analysis, Methylene Blue, Method Validation, Spectrophotometer