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Stabilitas Parameter Kualitas 35 Klon Teh Sinensis (Camellia Sinensis var. Sinensis) Yang Diolah Menjadi Teh Hijau Dengan Metode Panning dan Steaming Prayoga, M. Khais; Syahrian, Heri; Rahadi, Vitria Puspitasari; Maulana, Hilman; Shabri, Shabri; Akhdya, Alina; Martono, Budi; Santoso, Tri Joko; Utami, Dwinita Wikan
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 2 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i2.505

The Research Institute for Tea and Cinchona (RITC) has a collection of 35 tea clones of the Sinensis type whose performance parameters for green tea are unknown, so it is necessary to screen these clones as an effort to characterize the performance parameters for green tea quality. The purpose of this study was to determine the clones with stable quality parameter performance in two processing methods namely panning and steaming as well as the suitability of each clone with the processing method. The diversity of green tea quality parameter performance was analyzed using principal component analysis (PCA). The stability of the green tea quality parameters was tested using the Finaly-Wilkinson method, while the differences in the performance of the green tea quality parameters in the panning and steaming methods were analyzed using combined variance followed by an additional Honest Significant Difference (BNJ) test at the 5% level. The results of this study were clones I.1.101, I.2.34, I.2.85, II.1.3, II.1.60, R1, S2, GMBS 3, and GMBS 4 which had stable green tea quality in two processing methods, namely panning and steaming. . Meanwhile clones I.2.34, I.4.113, II.2.146, II.3.16, R3, S1, S3, and GMBS 3 will show good quality green tea when processed using the panning method, while clones I.1.93, II.2.108 , II.4.32, II.4.178, SGMBA, and Yabukita will show good quality green tea when processed using the steaming method.

Factors Affecting the Business Development of Women with Disabilities in Indonesia Akhmad, Khabib Alia; Santoso, Tri Joko
Indonesian Journal of Disability Studies Vol. 10 No. 1 (2023)
Publisher : The Center for Disability Studies and Services Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijds.2023.010.01.08

The existence of Micro, Small and Medium Enterprises (MSMEs) in Indonesia has had a significant impact on the nation's economy. Various types of MSMEs have been developed, including MSMEs with disabilities, especially those who are female. This study was conducted to determine the factors that influence the development of women's MSMEs, especially those with disabilities. The research method used was descriptive and qualitative. Data were collected through in-depth interviews, observations, structured interviews, and FGDs. The results show that there are several factors that influence the development of MSMEs for women with disabilities, namely physical accessibility and information, education and training, social support and networks, access to capital and funding, inclusion policies and legal protection, and awareness and attitude change. In addition, internal factors originating from female MSME actors with disabilities, and external factors originating from family, community, and government also influence the development of women with MSMEs with disabilities.

Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi Santoso, Tri Joko; Husni, Ali; Nugroho, Kristianto; Ya’la, Zakirah Raihani; Dewi, Triyani; Marhawati, Marhawati; Maemunah, Maemunah; Rosyida, Eka; Ndobe, Samliok
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1062

Seaweed is a fishery commodity that has high economic value because it contains carrageenan which can be used in the pharmaceutical, food, cosmetics, and industrial fields. Central Sulawesi Province is the second largest seaweed producer in Indonesia after South Sulawesi Province. The genetic diversity of seaweed in Central Sulawesi has not been studied much. Therefore, molecular-based characterization activities to identify the genetic variation of seaweed in the province are urgently needed. The purpose of the study was to optimize PCR techniques based on targeted start codon markers (SCoT markers) to identify the genetic diversity of seaweed accessions from Central Sulawesi Province. The results showed that the parameters for seaweed PCR amplification have been optimized. Of the fifteen SCoT primers, 10 of them can work to amplify seaweed DNA collected from several regions in Central Sulawesi. This is shown by the production of PCR result amplicons. PCR results also indicate polymorphism from the seaweed samples tested, although some seaweed samples have not been successfully amplified. The parameters of the PCR technique still need to be improved, especially the concentration of DNA prints, so that valid PCR results will be obtained for use in identifying seaweed genetic diversity.

Molecular diversity of citrus genotypes using callose synthase 7 gene markers linked to Huanglongbing resistance Nugroho, Kristianto; Purwito, Agus; Sukma, Dewi; Kosmiatin, Mia; Santoso, Tri Joko; Husni, Ali; Martasari, Chaireni; Lestari, Puji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.64952

Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus, is a notable disease affecting citrus plantations globally. Several studies showed that the callose synthase 7 gene is crucial for the citrus defense system against this pathogen. The study aimed to analyze the nucleotide variations and genetic diversity among several citrus genotypes using specific gene primers designed from the callose synthase 7 gene sequence. Genomic DNA from eleven citrus genotypes was amplified using the specific primers, and Sanger sequencing was employed to identify the nucleotide sequence of the PCR products. The results revealed a total of 66 single-nucleotide polymorphisms (SNPs), 10 insertions, and 11 deletions were detected in callose synthase 7 gene fragment sequences. Of these, one out of five noteworthy SNPs identified at a position of 200 bp downstream of the START codon showed distinguishing features between susceptible and resistant/tolerant genotypes. Phylogenetic analysis clearly discriminated the eleven citrus genotypes into two clusters at a dissimilarity coefficient of 0.05, with all genotypes grouped in the first cluster, except for the Chinese box orange and orange jasmine. The identification of notable SNPs in this study can aid in developing new markers for the rapid selection of genotypes with enhanced HLB resistance in citrus breeding programs. Keywords: Candidatus Liberibacter asiaticus; nucleotide variations; Sanger sequencing; phylogenetic analysis; SNP

Nucleotide variations of WRKY70 gene sequence related to Huanglongbing resistance in citrus Nugroho, Kristianto; Purwito, Agus; Sukma, Dewi; Kosmiatin, Mia; Santoso, Tri Joko; Reflinur, Reflinur; Mastur, Mastur
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10092

Huanglongbing, inflicted by Candidatus Liberibacter asiaticus in Asia region, is a destructive disease affecting citrus productions worlwide. Several studies have identified resistance genes that play essential roles in the citrus defense system against this pathogen. The goals of this study were to design the specific gene primers from the WRKY70 gene sequence and analyze the nucleotide variations and genetic diversity among several citrus genotypes. Genomic DNA from nine citrus genotypes were amplified using WRKY70-specific gene primers and the products of PCR were sent to Sanger sequencing, while the sequences of the other 12 genotypes were collected from Citrus Genome Database. The results revealed a total of 282 nucleotide variations which consisted of 157 SNPs, 28 insertions, and 97 deletions, were identified in the WRKY70 gene fragment sequence. There were three notable SNPs detected, with only one SNP [C/T] in first intron area at the position of 524 bp downstream from START codon that showed its ability to distinguish between susceptible and tolerant/resistant citrus genotypes. The phylogenetic analysis also revealed the clearly separation among citrus genotypes in two main clusters. The discovery of this SNP is useful for designing a functional marker as a screening tool in citrus breeding program in the future.

Stabilitas Parameter Kualitas 35 Klon Teh Sinensis (Camellia Sinensis var. Sinensis) Yang Diolah Menjadi Teh Hijau Dengan Metode Panning dan Steaming Prayoga, M. Khais; Syahrian, Heri; Rahadi, Vitria Puspitasari; Maulana, Hilman; Shabri, Shabri; Akhdya, Alina; Martono, Budi; Santoso, Tri Joko; Utami, Dwinita Wikan
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 2 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i2.505

The Research Institute for Tea and Cinchona (RITC) has a collection of 35 tea clones of the Sinensis type whose performance parameters for green tea are unknown, so it is necessary to screen these clones as an effort to characterize the performance parameters for green tea quality. The purpose of this study was to determine the clones with stable quality parameter performance in two processing methods namely panning and steaming as well as the suitability of each clone with the processing method. The diversity of green tea quality parameter performance was analyzed using principal component analysis (PCA). The stability of the green tea quality parameters was tested using the Finaly-Wilkinson method, while the differences in the performance of the green tea quality parameters in the panning and steaming methods were analyzed using combined variance followed by an additional Honest Significant Difference (BNJ) test at the 5% level. The results of this study were clones I.1.101, I.2.34, I.2.85, II.1.3, II.1.60, R1, S2, GMBS 3, and GMBS 4 which had stable green tea quality in two processing methods, namely panning and steaming. . Meanwhile clones I.2.34, I.4.113, II.2.146, II.3.16, R3, S1, S3, and GMBS 3 will show good quality green tea when processed using the panning method, while clones I.1.93, II.2.108 , II.4.32, II.4.178, SGMBA, and Yabukita will show good quality green tea when processed using the steaming method.

Pembentukan Buah dan Perkecambahan Tanaman Jarak Pagar pada Transformasi Langsung Melalui Jalur Tabung Polen Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The genetic transformation via pollen-tube pathway in jatropha is the first alternative method that was applied in this plant. The objective of the research was to study fruit set and germination of three genotypes of jatropha following direct transformations via pollen-tube pathways. The research was conducted during April 2014 until January 2015 at jatropa’s experimental field, Pasuruan, and at green house of ICABIOGRAD, Bogor. Three genotypes of jatropha i.e., IP3A, IP3P and JcUMM18 were used. In the first experiment, split plot design was used where 3 levels of DNA plasmid concentration as a main plot and 5 levels of stigma-drip time of DNA plasmid as a subplot. In the second experiment randomized block design was used with single factor consisting of 15 combinations of concentration and stigma-drip time of DNA plasmid as treatments and control. The results demonstrated that interaction between concentration and application time of DNA plasmid did not significantly affect fruit and seeds formation of three Jatropha genotypes. Combination of DNA plasmid concentration with time of stigma-drip had significant effect on seed germination rate of IP3A genotype, but not significant on the other variables. The concentration of 0.05-0.5 µg µL-1 and application time of DNA plasmid at 1-10 hours after pollination could be applied on jatropha genetic transformation via pollen-tube pathways.Keywords: DNA plasmid, Jatropha curcas, pCAMBIA1301, pollen-tube, stigma-drip

Efficiency of Genetic Transformation via Pollen-Tube Pathway of Jatropha (Jatropha curcas L.) Based on Histochemical and Molecular Analysis Zainudin, Agus; Purwoko, Bambang Sapta; Santoso, Tri Joko; Ardie, Sintho Wahyuning; Trikoesoemaningtyas, and
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 3 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The genetic transformation via pollen-tube pathway is an alternative method to overcome the constraints imposed by genotype specificity in transformation and regeneration in jatropha (Jatropha curcas L.) tissue culture. Therefore, it is necessary to establish important parameters for efficient genetic transformation of jatropha via pollen-tube pathway. The objective of the research was to study the efficiency of direct transformation of jatropha via pollen-tube pathway based on histochemical and molecular analysis. Solution of purified pCAMBIA1301 DNA plasmid carrying a hptII marker gene and a gus reporter gene with concentration level of 0.05, 0.25, 0.50 µg µl-1 were applied to stigma of flowers at 1, 2, 4, 7, 10 h after pollination. Seedling of IP3A, IP3P and JcUMM18 jatropha’s genotypes derived from 15 combination treatments of plasmid DNA concentration and application time, also wild type was subjected to histochemical and molecular analyses. Based on those analyses, the efficiency of transformation via pollen-tube pathway of three jatropha genotypes ranged from 1.5-16.7%. PCR analysis showed that a number of positive plants were identified by using specific primers hptII and gus, i.e. 1-3 and 3-7 plants of the 15 combined treatments, respectively. It indicated that the transformation efficiency via the pollen-tube pathway varied in each jatropha genotype.Keywords: Jatropha curcas L., pCAMBIA1301, plasmid DNA, stigma-drip

OPTIMIZATION OF KINETIN CONCENTRATIONS AND MEDIUM COMPOSITIONS FOR CITRUS SHOOT MULTIPLICATION FROM COTILEDONARY NODE Nugroho, Kristianto; Kosmiatin, Mia; Santoso, Tri Joko; Sukma, Dewi; Purwito, Agus; Husni, Ali; Martasari, Chaireni
BIOTROPIA Vol. 31 No. 1 (2024): BIOTROPIA Vol. 31 No. 1 April 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.1.2136

Plant regeneration post-genetic transformation play an important an role in genome editing activities that should be optimized via several factors such as the composition of the medium and the concentration of plant growth regulators. This study aimed to optimize the kinetin concentrations and medium compositions for shoot multiplication originating from cotyledon node explants of several local citrus cultivars. The cotyledonary nodes from three citrus cultivars (Batu 55, Siam Madu, and Proksi-1 Agrihorti) were incubated in MS medium with Morel and Wetmore vitamins (VMW) supplemented with several kinetin concentrations (0; 0.2; 0.4; 0.6; 0.8; and 1 mg/L). The best kinetin concentrations for number of shoots variable were then combined with MT medium. The results showed that kinetin concentration at 0.8 mg/L gave the best number of shoot in Batu 55 cultivar as well as 1 mg/L concentration in Siam Madu and Proksi-1 Agrihorti cultivars. The combination 1 mg/L kinetin with Murashige and Tucker (MT) medium showed the best number of shoots, percentage of shoot formation, number of leaves, number of nodes, and shoot length in this study. This medium composition could be further used for shoot multiplication in genetic transformation in those three citrus cultivars, including genome editing activities in development of new improved citrus varieties.

Phenotypic Screening of Ds Transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayanti, Dwi; Pantouw, Carla Frieda; Astuti, Dwi; Indrayani, Sri; Rachmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
Annales Bogorienses Vol. 15 No. 1 (2011): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice, combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller.

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