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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Penelitian dan Pengembangan Pertanian Jurnal Tanaman Industri dan Penyegar Indonesian Journal of Disability Studies BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Biologi Tropis ANNALES BOGORIENSES AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Journal La Lifesci Makara Journal of Science
TRI JOKO SANTOSO
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertania
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Stabilitas Parameter Kualitas 35 Klon Teh Sinensis (Camellia Sinensis var. Sinensis) Yang Diolah Menjadi Teh Hijau Dengan Metode Panning dan Steaming Prayoga, M. Khais; Syahrian, Heri; Rahadi, Vitria Puspitasari; Maulana, Hilman; Shabri, Shabri; Akhdya, Alina; Martono, Budi; Santoso, Tri Joko; Utami, Dwinita Wikan
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 2 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i2.505

Abstract

The Research Institute for Tea and Cinchona (RITC) has a collection of 35 tea clones of the Sinensis type whose performance parameters for green tea are unknown, so it is necessary to screen these clones as an effort to characterize the performance parameters for green tea quality. The purpose of this study was to determine the clones with stable quality parameter performance in two processing methods namely panning and steaming as well as the suitability of each clone with the processing method. The diversity of green tea quality parameter performance was analyzed using principal component analysis (PCA). The stability of the green tea quality parameters was tested using the Finaly-Wilkinson method, while the differences in the performance of the green tea quality parameters in the panning and steaming methods were analyzed using combined variance followed by an additional Honest Significant Difference (BNJ) test at the 5% level. The results of this study were clones I.1.101, I.2.34, I.2.85, II.1.3, II.1.60, R1, S2, GMBS 3, and GMBS 4 which had stable green tea quality in two processing methods, namely panning and steaming. . Meanwhile clones I.2.34, I.4.113, II.2.146, II.3.16, R3, S1, S3, and GMBS 3 will show good quality green tea when processed using the panning method, while clones I.1.93, II.2.108 , II.4.32, II.4.178, SGMBA, and Yabukita will show good quality green tea when processed using the steaming method.
Factors Affecting the Business Development of Women with Disabilities in Indonesia Akhmad, Khabib Alia; Santoso, Tri Joko
Indonesian Journal of Disability Studies Vol. 10 No. 1 (2023)
Publisher : The Center for Disability Studies and Services Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijds.2023.010.01.08

Abstract

The existence of Micro, Small and Medium Enterprises (MSMEs) in Indonesia has had a significant impact on the nation's economy. Various types of MSMEs have been developed, including MSMEs with disabilities, especially those who are female. This study was conducted to determine the factors that influence the development of women's MSMEs, especially those with disabilities. The research method used was descriptive and qualitative. Data were collected through in-depth interviews, observations, structured interviews, and FGDs. The results show that there are several factors that influence the development of MSMEs for women with disabilities, namely physical accessibility and information, education and training, social support and networks, access to capital and funding, inclusion policies and legal protection, and awareness and attitude change. In addition, internal factors originating from female MSME actors with disabilities, and external factors originating from family, community, and government also influence the development of women with MSMEs with disabilities.
Optimization of PCR Analysis Based on Start Codon Targeted Markers (SCoT Markers) for Identification of Genetic Variation of Seaweed from Central Sulawesi Santoso, Tri Joko; Husni, Ali; Nugroho, Kristianto; Ya’la, Zakirah Raihani; Dewi, Triyani; Marhawati, Marhawati; Maemunah, Maemunah; Rosyida, Eka; Ndobe, Samliok
Journal La Lifesci Vol. 5 No. 1 (2024): Journal La Lifesci
Publisher : Newinera Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37899/journallalifesci.v5i1.1062

Abstract

Seaweed is a fishery commodity that has high economic value because it contains carrageenan which can be used in the pharmaceutical, food, cosmetics, and industrial fields. Central Sulawesi Province is the second largest seaweed producer in Indonesia after South Sulawesi Province. The genetic diversity of seaweed in Central Sulawesi has not been studied much. Therefore, molecular-based characterization activities to identify the genetic variation of seaweed in the province are urgently needed. The purpose of the study was to optimize PCR techniques based on targeted start codon markers (SCoT markers) to identify the genetic diversity of seaweed accessions from Central Sulawesi Province. The results showed that the parameters for seaweed PCR amplification have been optimized. Of the fifteen SCoT primers, 10 of them can work to amplify seaweed DNA collected from several regions in Central Sulawesi. This is shown by the production of PCR result amplicons. PCR results also indicate polymorphism from the seaweed samples tested, although some seaweed samples have not been successfully amplified. The parameters of the PCR technique still need to be improved, especially the concentration of DNA prints, so that valid PCR results will be obtained for use in identifying seaweed genetic diversity.
Molecular diversity of citrus genotypes using callose synthase 7 gene markers linked to Huanglongbing resistance Nugroho, Kristianto; Purwito, Agus; Sukma, Dewi; Kosmiatin, Mia; Santoso, Tri Joko; Husni, Ali; Martasari, Chaireni; Lestari, Puji
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.64952

Abstract

Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus, is a notable disease affecting citrus plantations globally. Several studies showed that the callose synthase 7 gene is crucial for the citrus defense system against this pathogen. The study aimed to analyze the nucleotide variations and genetic diversity among several citrus genotypes using specific gene primers designed from the callose synthase 7 gene sequence. Genomic DNA from eleven citrus genotypes was amplified using the specific primers, and Sanger sequencing was employed to identify the nucleotide sequence of the PCR products. The results revealed a total of 66 single-nucleotide polymorphisms (SNPs), 10 insertions, and 11 deletions were detected in callose synthase 7 gene fragment sequences. Of these, one out of five noteworthy SNPs identified at a position of 200 bp downstream of the START codon showed distinguishing features between susceptible and resistant/tolerant genotypes. Phylogenetic analysis clearly discriminated the eleven citrus genotypes into two clusters at a dissimilarity coefficient of 0.05, with all genotypes grouped in the first cluster, except for the Chinese box orange and orange jasmine. The identification of notable SNPs in this study can aid in developing new markers for the rapid selection of genotypes with enhanced HLB resistance in citrus breeding programs.   Keywords: Candidatus Liberibacter asiaticus; nucleotide variations; Sanger sequencing; phylogenetic analysis; SNP
Nucleotide variations of WRKY70 gene sequence related to Huanglongbing resistance in citrus Nugroho, Kristianto; Purwito, Agus; Sukma, Dewi; Kosmiatin, Mia; Santoso, Tri Joko; Reflinur, Reflinur; Mastur, Mastur
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10092

Abstract

Huanglongbing, inflicted by Candidatus Liberibacter asiaticus in Asia region, is a destructive disease affecting citrus productions worlwide. Several studies have identified resistance genes that play essential roles in the citrus defense system against this pathogen. The goals of this study were to design the specific gene primers from the WRKY70 gene sequence and analyze the nucleotide variations and genetic diversity among several citrus genotypes. Genomic DNA from nine citrus genotypes were amplified using WRKY70-specific gene primers and the products of PCR were sent to Sanger sequencing, while the sequences of the other 12 genotypes were collected from Citrus Genome Database. The results revealed a total of 282 nucleotide variations which consisted of 157 SNPs, 28 insertions, and 97 deletions, were identified in the WRKY70 gene fragment sequence. There were three notable SNPs detected, with only one SNP [C/T] in first intron area at the position of 524 bp downstream from START codon that showed its ability to distinguish between susceptible and tolerant/resistant citrus genotypes. The phylogenetic analysis also revealed the clearly separation among citrus genotypes in two main clusters. The discovery of this SNP is useful for designing a functional marker as a screening tool in citrus breeding program in the future.
Co-Authors A.A. Ketut Agung Cahyawan W Agus Purwito Akhdya, Alina Akhmad, Khabib Alia Ali Husni Andari Risliawati, Andari Andi Salamah Aniversari Apriana Anky Zannati Apriadi Situmorang Atmitri Sisharmini Budi Martono Chaerani Chaerani Chaireni Martasari Dewi Sukma Dwi Astuti Dwi Widyajayantie DWINITA WIKAN UTAMI Eka Rosyida Enny Randriani Enung Sri Mulyaningsih Heri Syahrian, Heri Hilman Maulana Irshanty, Firdha Meidi KRISTIANTO NUGROHO, KRISTIANTO Kurniawan Rudi Trijatmiko, Kurniawan Rudi M. Khais Prayoga, M. Khais Maemunah Maemunah Marhawati, Marhawati Mastur Mastur Mia Kosmiatin Nurul Hidayatun Pantouw, Carla F. Priyono, Dika Migi PUJI LESTARI REFLINUR REFLINUR Samliok Ndobe Satya Nugroho Shabri Shabri, Shabri Solly Aryza Sri Indrayani, Sri Syafaruddin . Syamsidah Rahmawati, Syamsidah Triyani Dewi Vincentia Esti Windiastri, Vincentia Esti Vitria Puspitasari Rahadi Zakirah Raihani Ya’la