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RESPON PERTUMBUHAN MISELIA G3 FP005 FAPERTA UNSIKA PADA MEDIA BIAKAN MURNI YANG BERBEDA Astri Dwi Widianingsih; Bastaman Syah; Vera Oktavia Subardja; Ani Lestari
Jurnal Agrotech Vol. 14 No. 2 (2024)
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/agrotech.v14i2.181

Abstract

The Indonesian region has quite high air humidity and is ideal for the growth of straw mushrooms. To obtain pure cultures of straw mushrooms, you can go through the pure culture or G0 culture stage, then from G0 sub-cultures are carried out into G1, G2, G3 and G4. This research aims to obtain the best pure culture media for the growth of G3 FP005 Faperta Unsika mycelia. The research was carried out at the Biotechnology and Plant Breeding Laboratory, Faculty of Agriculture, Singaperbangsa University, Karawang, from April to May 2023. The research method used was a single factor Completely Randomized Design (CRD) with 7 treatments and 5 replications: PDA 100% (A), Charcoal Husk 100 % (B), 100% Husk (C), 75% PDA + 25% Husk Charcoal (D), 80% PDA + 20% Husk Charcoal (E), 50% Husk Charcoal + 50% Husk Charcoal (F), and PDA 50% + Charcoal Husk 25% + Husk 25% (G). Treatments were analyzed using variance and F test at 5% level, to find out the best treatment followed by further DMRT (Duncan Multiple Range Test) at 5% level. The results of the research were that there was a real effect of using several pure cultures on the growth of G3 FP005 mycelia. PDA 75% + Charcoal Husk 25% media gave the highest diameter at 2 dai (2.24 cm), 3 dai (4.78) and 4 dai (7.19 cm), the highest growth rate was (2.52 cm), diameter regression with R2 0.898 then P value 0.052047, and growth rate regression with R2 0.68 then P value 0.410604.
Performance Test of Vegetative Characteristics of Crossed Rice (Oryza sativa L.) Lines of Ciherang Variety X B11143D Line in Telagasari, Karawang Regency, Indonesia Damayanti, Utari; Lestari, Ani; Subardja, Vera Oktavia; Hadiarto, Toto; Enggarini, Wening
Agro Bali : Agricultural Journal Vol 7, No 2 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i2.1721

Abstract

One way to increase the genetic diversity of Ciherang as the superior variety is to cross Ciherang with the donor B11143D line as a New Plant Type (NPT) rice. This study aimed to obtain Ciherang X B11143D lines with the best vegetative characteristics in the field. The research was conducted in rice fields in Talagasari Village, Telagasari District, Karawang Regency, from May to September 2023. This experiment used a single-factor Randomized Block Design (RBD) with three replications of 22 treatments, consisting of 19 Ciherang X B11143D lines and three comparison varieties. The effect of treatment was studied using analysis of variance. The results showed that the rice lines significantly influenced the vegetative characters of Ciherang X B11143D lines in Telagasari, Karawang Regency. Based on the observed characters, several lines were selected i.e. 124.2.3, 94.3.3, and 20.4.4 lines. Those three lines chosen as backcrossing lines performed similarly compared to Ciherang as recurrent parent and inherited several important traits for rising productivity from the B11143D line as donor parent, namely the length and area of flag leaf, the total number of tillers, and the number of productive tillers, which were significantly higher than Ciherang.
Kontaminasi Kadmium (Cd) Terhadap Kerapatan Azotobacter dan Pseudomonas Pada Media Tanam Eceng Gondok (Eichhornia Crassipes) Vera Oktavia Subardja; Elsi Diannitta Sinaga; Bastaman Syah
Jurnal Ilmiah dan Kajian Akademik Vol 3 No 02 (2025): Desember 2025
Publisher : Badan Perencanaan Pembangunan Daerah

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Abstract

Kadmium (Cd) merupakan salah satu logam berat yang berada di lingkungan serta memiliki efek toksik yang tinggi dalam konsentrasi yang rendah. Lingkungan yang terkontaminasi Cd dapat diperbaiki kembali dengan sistem pengolahan limbah logam berat dengan pendekatan bioremediasi. Bakteri penghasil eksopolisakarida (EPS) yang berperan dalam mengkelat logam dapat digunakan sebagai agen bioremediasi seperti Azotobacter dan Pseudomonas. Penelitian bertujuan untuk menunjukkan efektivitas pupuk hayati terhadap populasi Azotobacter dan Pseudomonas pada media yang terkontaminasi logam berat Cd. Metode yang digunakan adalah metode eksperimental dengan Rancangan Acak Kelompok (RAK) faktor tunggal yang terdiri atas 9 perlakuan dengan 3 kali ulangan. Perhitungan data dianalisis menggunakan analisis uji F pada taraf 5%. Apabila hasil uji F perlakuan menunjukkan hasil perbedaan yang berbeda nyata, maka dilakukan analisis uji lanjut Duncan Multiple Range Test (DMRT) pada taraf 5%. Hasil penelitian menunjukkan bahwa perlakuan berpengaruh nyata terhadap populasi Azotobacter dan Pseudomonas, serta akumulasi Cd pada akar. Perlakuan D (8 ppm Cd (25 ml Azotobacter)) memberikan hasil tertinggi terhadap populasi total Azotobacter (407,547 CFU/ml) dan akumulasi logam Cd pada akar paling rendah (0,649 mg/kg). Sedangkan perlakuan B (8 ppm Cd (50 ml/l Pseudomonas + 25 ml/l Azotobacter) memberikan hasil tertinggi terhadap populasi total Pseudomonas (353,703 CFU/ml). Perlakuan Inokulasi pupuk hayati Azotobacter dan Pseudomonas mampu resisten terhadap media yang terkontaninasi logam Cd hingga 16 ppm, mampu menurunkan akumulasi logam Cd pada akar tanaman, dan dapat digunakan dalam bioremediasi logam Cd pada media yang terkontaminasi Cd.
Pengaruh Pupuk Hayati Terhadap Pertumbuhan Tanaman Apu-Apu (Pistia stratiotes L.) pada Media yang Diberi Paparan Logam Timbal (Pb) Vera Oktavia Subardja; Fanny Maharani Putri; Bastaman Syah
Jurnal Ilmiah dan Kajian Akademik Vol 3 No 02 (2025): Desember 2025
Publisher : Badan Perencanaan Pembangunan Daerah

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Abstract

Pencemaran air oleh logam berat menyebabkan kualitas air menurun dan mengancam kelangsungan hidup organisme karena dapat menimbulkan efek toksik. Memanfaatkan agen hayati seperti Azotobacter sp. dan Pseudomonas sp. dapat digunakan sebagai cara alternatif untuk menanggulangi pencemaran air dan mendukung pertumbuhan tanaman. Penelitian ini bertujuan untuk mendapatkan kombinasi pupuk hayati yang tepat terhadap pertumbuhan tanaman apu-apu (Pistia stratiotes L.) pada media tanam yang terkontaminasi logam berat. Penelitian dilaksanakan di rumah kaca Universitas Singaperbangsa Karawang, Jalan HS. Ronggo Waluyo, Telukjambe Timur, Karawang. Metode yang digunakan adalah Rancangan Acak Kelompok (RAK) faktor tunggal dengan 9 perlakuan dan 3 ulangan sehingga terdapat 27 unit percobaan terdiri dari: A (kontrol tanpa Pb dan tanpa pupuk hayati), B (10 ppm Pb (50 ml Pseudomonas sp. + 25 ml Azotobacter sp.)), C (10 ppm Pb (50 ml Pseudomonas sp.)), D (10 ppm Pb (25 ml Azotobacter sp.)), E (10 ppm Pb tanpa pupuk hayati), F (20 ppm Pb (75 ml Pseudomonas sp. + 50 ml Azotobacter sp.)), G (20 ppm Pb (75 ml Pseudomonas sp. + 50 ml Azotobacter sp.)), H (20 ppm Pb (50 ml Azotobacter sp.)), I (20 ppm Pb tanpa pupuk hayati). Hasil percobaan dianalisis dengan uji F, apabila berpengaruh nyata maka diuji lanjut Duncan Multiple Range Test (DMRT) taraf 5%. Hasil penelitian menunjukkan bahwa perlakuan B (10 ppm Pb (50 ml Pseudomonas sp. + 25 ml Azotobacter sp.)) berpengaruh nyata terhadap semua parameter pengamatan. Perlakuan ini memberikan hasil terbaik pada tinggi tanaman (17,523 cm), jumlah daun (38,443 helai), panjang akar (32,256 cm).
Pengkayaan Unsur P Pada Pupuk Organik Limbah Budidaya Jangkrik Untuk Tanaman Kacang Tanah (Arachis hypogaea L.) Putri, Nani Fauziyyah; Subardja, Vera Oktavia; Syah, Bastaman
Jurnal Agroekoteknologi Tropika Lembab Vol 8, No 2 (2026): Agroekoteknologi Tropika Lembab Volume 8 Nomor 2 Februari 2026
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jatl.8.2.2026.22050.29-37

Abstract

The utilization of cricket farming waste as a base material for organic fertilizer enriched with various phosphorus (P)sources aims to fulfill the phosphorus requirements of peanut plants (Arachis hypogaea L.) and thereby enhance crop production.This study aimed to determine the best combination of phosphorus-enriched organic fertilizer based on cricket farming waste forpeanut cultivation. The treatments consisted of: K1 = SP36 fertilizer; K2 = 20 tons/ha of cricket waste organic fertilizer; K3 = 20tons/ha of cricket waste organic fertilizer enriched with 5% SP36; K4 = 20 tons/ha of cricket waste organic fertilizer enrichedwith 5% rock phosphate; and K5 = 20 tons/ha of cricket waste organic fertilizer enriched with 50 ml of Pseudomonas-basedbiofertilizer. The observed data were analyzed using ANOVA at the 5% significance level, and if significant differences werefound, Duncan’s Multiple Range Test (DMRT) at the 5% level was applied. The K5 treatment produced the highest results inplant height across all observation times, number of leaves at 14 days after planting (dap), flowering duration, number of filledpods, weight of filled pods, and seed weight. The K2 treatment showed the highest number of leaves and branches at 21 and 28days after planting (dap).Keywords: enrichment, cricket farming waste organic fertilizer, rock phosphate, Pseudomonas
PENERAPAN FUN FARMING DALAM MENDUKUNG CONTRACT FARMING DI KABUPATEN PURWAKARTA Mahatmayana, I Ketut Manu; Supriadi, Devie Rienzani; Subardja, Vera Oktavia; Syabena, Muhammad Farrel; Umadi, Sarah Sakinah
JMM (Jurnal Masyarakat Mandiri) Vol 10, No 1 (2026): Februari
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i6.34936

Abstract

Abstrak: Kegiatan pengabdian masyarakat ini bertujuan untuk menerapkan konsep fun farming sebagai upaya mendukung keberhasilan contract farming di Kabupaten Purwakarta. Fun farming dipilih sebagai pendekatan karena mampu menggabungkan aspek edukasi, rekreasi, dan praktik langsung yang dapat meningkatkan pengetahuan, keterampilan, serta motivasi petani dalam mengelola usaha tani secara berkelanjutan. Metode pelaksanaan kegiatan meliputi sosialisasi mengenai pentingnya contract farming, pelatihan teknis budidaya tanaman hortikultura berbasis teknologi sederhana, serta pendampingan intensif kepada kelompok tani yang menjadi mitra dalam pola kemitraan tersebut. Kegiatan ini dihadiri sebanyak 40 orang mitra. Hasil kegiatan menunjukkan menunjukkan adanya peningkatan signifikan pada ketiga aspek tersebut, masing-masing pembuatan media tanam sebesar 26%, penanaman benih 23%, dan pemindahan bibit ke polybag sebesar 15%. Selain itu, kapasitas produksi bibit meningkat dari 2.000 menjadi 10.000 bibit setelah pelatihan. Kegiatan ini terbukti efektif dalam meningkatkan kompetensi dan produktivitas mitra. Dengan demikian, fun farming berperan penting sebagai strategi inovatif dalam memperkuat sistem contract farming yang berorientasi pada peningkatan produktivitas, keberlanjutan usaha, dan penguatan ekonomi lokal masyarakat. Abstract: This community service activity aims to implement the concept of fun farming as an effort to support the success of agricultural contracts in Purwakarta Regency. Fun farming was chosen as an approach because it can combine educational, recreational, and hands-on aspects that can enhance farmers' knowledge, skills, and motivation in managing their farms sustainably. The implementation methods for the activities include socialization about the importance of agricultural contracts, technical training on horticultural crop cultivation based on simple technology, and intensive mentoring for farmer groups who are partners in the partnership model. This activity was attended by 40 partners. The results of the activity show a significant increase in all three aspects: soil media preparation by 26%, seed planting by 23%, and seedling transfer to polybags by 15%. Additionally, seedling production capacity increased from 2,000 to 10,000 seedlings after the training. This activity has proven effective in improving the competence and productivity of partners. Thus, enjoyable agriculture plays an important role as an innovative strategy in strengthening contract farming systems aimed at increasing productivity, business sustainability, and strengthening the local economy of the community.
Properties of Azotobacter as biofertilizer and biocontrol of Fusarium Reginawanti Hindersah; Vera Oktavia Subardja; Pujawati Suryatmana; Rija Sudirja
AGROLOGIA: Jurnal Ilmu Budidaya Tanaman Vol 13 No 1 (2024): Agrologia : Jurnal Ilmu Budidaya Tanaman
Publisher : Fakultas Pertanian, Universitas Pattimura Ambon, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/agrologia.v13i1.12761

Abstract

The Nitrogen-fixer bacteria, Azotobacters, are the potential Plant Growth Promotion Rhizobacteria for increasing plant growth and productivity due to their prominent role as biofertilizer dan bioprotectant. The objective of the experiment was to identify the plant-growth related characteristic of various Azotobacter isolates and to test their antagonism to soil-borne pathogen Fusarium. The five Azotobacter isolates were grown in Nitrogen-free slant for determining their ability to fix the Nitrogen, producing phytohormones and exopolysaccharides, and to inhibit the growth of Fusarium oxysforum f.sp. batatas. The results verified that all isolates synthesize various concentration of Indole acetic acid, Gibberellin, Zeatin and Kinetin as well exopolysaccharide in the N-free media. However, this study found that Azotobacter AzV1 did not detected to fix the N. This study suggests that Azotobacter has a dual function as biofertilizer and bioprotectant.