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All Journal Majalah Kedokteran Bandung ODONTO Dental Journal Padjadjaran Journal of Dentistry Padjadjaran Journal of Dental Researchers and Students Odonto dental journal
Ame Suciati Setiawan
Departemen Oral Biologi-Farmakologi Fakultas Kedokteran Gigi Universitas Padjadjaran
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Efektivitas antijamur ekstrak kulit buah naga merah (Hylocereus polyrhizus) terhadap Candida albicans ATCC 10231: Studi eksperimental Salsabila, Syifa Ainun Fatiha; Setiawan, Ame Suciati; Malinda, Yuti
Padjadjaran Journal of Dental Researchers and Students Vol 9, No 3 (2025): Oktober 2025
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v9i3.65556

Abstract

ABSTRAKPendahuluan: Kandidiasis oral akibat Candida albicans merupakan masalah umum di bidang kedokteran gigi. Kulit buah naga merah (Hylocereus polyrhizus) diketahui mengandung senyawa bioaktif seperti flavonoid dan fenolik yang berpotensi sebagai antijamur. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak kulit buah naga merah terhadap Candida albicans ATCC 10231 secara in vitro. Metode: Studi eksperimental ini menggunakan metode difusi sumur (well diffusion). Ekstrak diuji dalam dua volume (20 µL dan 100 µL) dan lima konsentrasi (50%, 25%, 12,5%, 6,25%, 3,125%). Nystatin 100.000 IU/mL digunakan sebagai kontrol positif, dan DMSO 2,5% sebagai kontrol negatif. Identifikasi mikroorganisme dilakukan melalui CHROMagar dan pewarnaan hematoksilin eosin. Hasil: Tidak menunjukan zona hambat pada seluruh perlakuan ekstrak. Sebaliknya, Nystatin menunjukkan zona hambat rerata di atas 17 mm, sementara DMSO tidak menunjukkan efek hambatan. Identifikasi morfologi mengkonfirmasi mikroorganisme uji sebagai Candida albicans. Simpulan: Ekstrak kulit buah naga merah tidak menunjukkan aktivitas antijamur terhadap Candida albicans dalam metode difusi sumur (well diffusion). Penelitian lanjutan diperlukan dengan pendekatan dan metode yang berbeda.KATA KUNCI: Candida albicans, buah naga merah, antijamur, flavonoid, difusi sumur.Antifungal effectiveness of red dragon fruit peel extract (Hylocereus polyrhizus) against candida albicans ATCC 10231: study experimentalABSTRACTIntroduction: Oral candidiasis caused by Candida albicans is a common issue in dental medicine. Red dragon fruit peel (Hylocereus polyrhizus) is known to contain bioactive compounds such as flavonoids and phenolics, which have potential antifungal properties. This study aimed to evaluate the antifungal effectiveness of red dragon fruit peel extract against Candida albicans ATCC 10231 in vitro. Methods: This experimental study employed the well diffusion method. The extract was tested in two volumes (20 µL and 100 µL) and five concentrations (50%, 25%, 12,5%, 6,25%, 3,125%). Nystatin 100,000 IU/mL was used as a positive control, and 2.5% DMSO as a negative control. Microorganism identification was conducted using CHROMagar and hematoxylin-eosin staining. Results: No inhibition zones were observed in any of the extract treatments.. In contrast, Nystatin showed consistent inhibition zones with an average diameter above 17 mm, while DMSO showed no inhibitory effect. Morphological identification confirmed the test microorganism as Candida albicans. Conclusions: Red dragon fruit peel extract did not exhibit antifungal activity against Candida albicans in the well diffusion method. Further research is needed using different approaches and methodologies.KEY WORDS: Candida albicans, red dragon fruit, antifungal, flavonoid, well diffusion
Efektivitas antijamur ekstrak aril buah delima terhadap jamur Candida albicans ATCC 10231 secara in vitro: studi eksperimental Syafiq, Ikram; Setiawan, Ame Suciati; Malinda, Yuti
Padjadjaran Journal of Dental Researchers and Students Vol 9, No 3 (2025): Oktober 2025
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v9i3.65436

Abstract

ABSTRAKPendahuluan:  Delima (Punica granatum L.) merupakan salah satu tanaman herbal yang banyak digunakan sebagai obat tradisional dan telah diketahui mengandung senyawa alkaloid, saponin, flavonoid, punicalagin, fenolik dan tanin yang berfungsi sebagai antijamur. Mengingat adanya kebutuhan akan alternatif antijamur dengan efek samping minimal dan potensi aril buah delima sebagai antimikroba dan antijamur yang signifikan. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak aril buah delima terhadap C. albicans yang merupakan jamur penyebab oral candidiasis. Metode: Ekstrak aril buah delima diperoleh melalui maserasi kemudian diencerkan hingga mendapatkan konsentrasi 50%, 25%, 12,5%, 6,25%, dan 3,12%. Uji aktivitas antijamur dilakukan menggunakan metode difusi agar dengan cara mengukur diameter zona hambat. Hasil pengukuran diambil nilai rata-ratanya untuk melihat pengaruh konsentrasi ekstrak aril buah delima terhadap pertumbuhan C. albicans. Hasil: Hasil penelitian menunjukkan bahwa ekstrak aril buah delima konsentrasi 50%, 25%, 12.5%, 6.25% dan 3.12% tidak menghasilkan zona hambat. Simpulan: Berdasarkan hasil penelitian dapat diperoleh simpulan bahwa ekstrak aril buah delima tidak memiliki daya hambat terhadap C. albicans.KATA KUNCI: Oral candidiasis, ekstrak aril buah delima, antijamur, C. albicans, diameter zona hambat.Antifungal efficacy of pomegranate aril extract against Candida albicans ATCC 10231 in vitro: an experimental study  ABSTRACT Introduction: Pomegranate (Punica granatum L.) is one of the herbal plants that is widely used as a traditional medicine and has been known to contain alkaloids, saponins, flavonoids, punicalagin, phenolics and tannins that function as antifungals. Given the need for alternative antifungals with minimal side effects and the potential of pomegranate aril as significant antimicrobials and antifungals. This study aims to determine the inhibitory power of pomegranate aril extract against C. albicans which is a fungus that causes oral candidiasis. Methods: The antifungal activity test was carried out using the agar diffusion method by measuring the diameter of the inhibition zone. The average value of the measurement results was taken to see the effect of the concentration of pomegranate aril extract on the growth of C. albicans. Results: The results showed that pomegranate aril extract concentrations of 50%, 25%, 12.5%, 6.25% and 3.12% did not produce an inhibition zone Conclusions: Based on the results of the study, it can be concluded that pomegranate aril extract has no inhibitory power against C. albicans.KEY WORDS: Oral candidiasis, pomegranate aril extract, antifungal, C. albicans, inhibitory zone diameter.
KNOWLEDGE ON INFECTION PREVENTION AND CONTROL AMONG STUDENTS IN UNIVERSITAS PADJADJARAN DENTAL HOSPITAL Paham, Aulia Narendra Mohamad; Malinda, Yuti; Setiawan, Ame Suciati
Odonto : Dental Journal Vol 9, No 1 (2022): July 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.1.100-109

Abstract

Background: Dentist is one of the professions that are vulnerable to the transmission of infectious diseases there is hence it requires proper infection control. The educational hospital is responsible for carrying out infection control training that includes appropriate standard precautions for dental students before carrying out educational activities in the hospital so that students are ready to treat patients. This study aims to determine the knowledge of interns and residents at Universitas Padjadjaran Dental Hospital on infection prevention and control.Method: This is a quantitative descriptive study with a cross-sectional approach using data from the training section of Universitas Padjadjaran Dental Hospital from August 2020 to February 2021. Determination of the number of samples taken using the Isaac and Michael formula and using a proportionate stratified random sampling technique.Result: The average value of interns and residents are the same, which is 71, seen also from the average percentage of those who answered correctly on the questionnaire which showed similar results between interns and residents. Conclusion: Knowledge on infection prevention and control among interns and residents are the same, with knowledge classified in the sufficient category.
AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Setiawan, Ame Suciati; Fatriadi, Fajar; Prisinda, Diani
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.111-116

Abstract

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.
Co-Authors Diani Prisinda Elin Yulinah Emma Rachmawati Fajar Fatriadi Hajanajumudin, Hanizul Hikmat Permana I Ketut Adnyana Kadek Seruni Kusumanegara, Kadek Seruni Lui Dwen Tjin, Lui Dwen Mieke Hemiawati Satari Paham, Aulia Narendra Mohamad Primal Sudjana Salsabila, Syifa Ainun Fatiha Susilo, Ivhatry Rizky octavia putri Syafiq, Ikram Widyawati, Hanna Yuliawati Zenab, Yuliawati Yuti Malinda